Pnoc (NM_001205075) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	N/O; N/OFQ; N23; Np; Npnc1; OFQ; OFQ/N; p
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226334 representing NM_001205075 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATCCTCTTTTGTGACGTTCTGCTGCTCAGCCTGCTCTCCAGCGTGTTCAGCAGCTGTCCCAGGG ACTGCCTCACCTGCCAGGAGAAGCTCCACCCAGCTCCAGACAGCTTCAACTTAAAGACGTGCATCCTCCA GTGTGAAGAGAAGGTCTTCCCCCGCCCTCTCTGGACTGTATGCACCAAAGTCATGGCCAGTGGCTCCGGG CAGCTCAGCCCTGCTGACCCAGAGCTTGTGTCAGCTGCTCTTTACCAGCCAAAGGCCTCGGAGATGCAGC ACCTGAAGAGAATGCCGCGTGTCCGGAGCTTGGTGCAAGTGCGAGATGCAGAGCCTGGCGCAGATGCTGA GCCTGGCGCAGATGCTGAGCCTGGCGCAGATGACGCTGAGGAGGTGGAGCAGAAGCAGCTGCAGAAAAGG TTTGGGGGCTTCACCGGGGCCCGGAAATCAGCCCGGAAGTTGGCCAACCAGAAGCGGTTCAGTGAGTTTA TGAGGCAGTACCTGGTCCTGAGCATGCAGTCAAGTCAACGCCGGCGCACCCTGCACCAGAATGGCATCCA GGTGATCCCCCGTACAGCATGTGTCCACTCCAAGACCTGCAGGCCGGGAGTCAGGATTCCTCCCTCCCCG AGGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205075
Insert Size	639 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205075.1, NP_001192004.1
RefSeq Size	2101 bp
RefSeq ORF	639 bp
Locus ID	18155
UniProt ID	Q64387
Gene Summary	This gene encodes the precursor for neuropeptides that have been implicated in a wide range of physiological roles such as transmission and sensitivity to pain, learning, memory, anxiety and depression, in the central nervous system. The encoded protein is a precursor that is proteolytically processed to generate multiple biologically active peptides including nociceptin and nocistatin which have opposite functions in pain transmission. Mice lacking the encoded protein display increased anxiety, elevated basal pain threshold and impaired adaptation to repeated stress. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate exon compared to variant 1. This results in a frameshift, and a longer isoform (2, also known as N27K) with a distinct C-terminus, compared to isoform 1.

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