Pmm1 (NM_001282041) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C77612
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226280 representing NM_001282041 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGCTCCTCCCCGTGCTGCGCAGCTGGCCCCGCCCCGGCCCCGCCCGGCCTCTGTCGCACGCG ACCCTGGCGGTGGCTGCAGCGACAGCGCTTGGCGCGCGGAGCTACAGCCATGGCAGTCGCCGTCGAGGGC GCCCGCAGGAAAGAGCGCATCCTCTGCCTGTTTGACGTGGACGGGACCCTCACGCCAGCTCGCCAGAAAA TTGACCCTGAGGTATCGGCCTTCCTGCAGAAGCTACGAAGCAGGCTGCACCCTGGAGGAGAGGATCGAGT TCTCGGAACTGGACAAGAAGGAGAAGATCCGGGAGAAGTTTGTGGAAGCCTTGAAGACAGAGTTTGCTGG CAAGGGGCTGCGGTTCTCCCGAGGAGGCATGATAAGCTTCGATGTCTTCCCCGAGGGCTGGGATAAGCGC TACTGCCTGGACAGCCTGGATGAAGACAGCTTTGACATCATCCACTTCTTTGGAAATGAGACCAGTCCTG GCGGGAATGACTTTGAGATCTATGCGGACCCCCGGACTGTCGGCCATAGCGTGGTCTCCCCTCAGGACAC TGTACAGCGATGCCGTGAGCTCTTCTTCCCAGAGACAGCCCACGAGGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282041
Insert Size	612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282041.1, NP_001268970.1
RefSeq Size	1020 bp
RefSeq ORF	612 bp
Locus ID	29858
Gene Summary	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences in the 5' coding region and initiates translation at an alternate start codon, which results in a translational frameshift, compared to variant 1. The encoded isoform (3) has a shorter and distinct N-terminus, compared to isoform 1.

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