Galt (NM_001302511) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW553376
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226275 representing NM_001302511 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGCTGTTCTAACCCCCATCCCCACTGCCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCC AGCGTGAAGAGCGATCCCAGCAGACCTATCACAGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCA CCAAGAGCTCCTCAGGAAGGAACGTCTGGTCCTAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGG GCAGTGTGGCCTTTCCAGACACTTCTGCTGCCCCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCG CTGAGCGTGATGATCTCGCCTCCATCATGAAGAAGCTCTTGACCAAGTACGACAATCTATTTGAGACATC CTTTCCCTACTCCATGGGCTGGCATGGGGCTCCCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGG CAGCTCCACGCCCACTACTACCCCCCACTTCTGCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATG AAATGCTTGCCCAGGCCCAGCGTGACCTCACTCCCGAACAGGCCGCAGAAAGATTAAGGGCGCTTCCCGA GGTACACTATTGCCTGGCGCAGAAAGACAAGGAAACGGCAGCCATTGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302511
Insert Size	612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302511.1, NP_001289440.1
RefSeq Size	1544 bp
RefSeq ORF	612 bp
Locus ID	14430
Gene Summary	The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) uses an alternate exon structure at the 5' end and utilizes a downstream start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2, 7, 8, and 9 all encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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