Rbpms (NM_001286168) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010300K22Rik; 2700019M19Rik; AU017537; hermes; RBP-MS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226184 representing NM_001286168 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGCGGCGGCAAAGCCGAGAAGGAGAACACCCCGAGCGAGGCCAACCTTCAGGAGGAGGAGGTCC GGACCCTATTTGTCAGCGGTCTGCCTCTGGACATCAAGCCCCGAGAGCTGTACCTGCTCTTCAGACCATT CAAGGGCTATGAAGGTTCTCTCATAAAGCTCACATCTAAACAGCCCGTAGGCTTTGTCAGTTTTGACAGT CGCTCAGAAGCAGAGGCCGCAAAGAACGCTTTGAACGGCATCCGCTTCGATCCTGAAATCCCGCAAACGC TACGACTAGAGTTTGCTAAGGCAAACACGAAGATGGCCAAGAACAAACTCGTAGGGACTCCAAACCCCAG TACTCCTCTGCCCAACACTGTACCTCAGTTCATTGCCAGGGAGCCATATGCGCTGGATCCCTCCCTCCGA GGCCACTTCCCAGGGCTGGAAGTCCCGGCAGTTCTGCTGAGGCTGTGTCTCGGGTGTGTGATGGCGGCTG CAATCTGTCTTGTGGGTATTAATGCGGTCTTCACTGGTGGCGACTGTCATCCAGCTGTTCTGCAAAACTG GAGCTTGCTGGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286168
Insert Size	576 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286168.1, NP_001273097.1
RefSeq Size	2435 bp
RefSeq ORF	576 bp
Locus ID	19663
Gene Summary	Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. This results in a shorter protein (isoform 4) with a distinct C-terminus, compared to isoform 1.

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