Uros (NM_001302086) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI415298; Ur; UROIIIS; Uros3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226160 representing NM_001302086 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTTCTCTTACTAAAAGACGCCAAGGAGGATGACAGCGGCCTGGATCCATATATCCAGGAGCTGC GATTGTGTGGCCTAGAAGCCACACTGATTCCTGTGCTGTCATTTGAGTTTATGTCTCTCCCCAGTTTGTC AGAAAAGCTGTCTCATCCTGAAGGCTTTGGAGGACTCATTTTCACCAGCCCCAGGGCAGTGGAAGCAGTG AAGCTGTGTTTGGAGAAGGACAATAAAACTGAAGCCTGGGAGAAGTCTCTGAAAGACAGATGGAATGCCA AGTCTGTGTACGTGGTTGGAAGTGCCACCGCTTCTCTAGTGAATAAAATTGGTCTGGATGCAGAAGGAGC GGGCAGTGGAAATGCAGAAAAGCTTGCTGAATATATTTGCTCAAATGGCCTCCTGGGTGAACAGCGATCC CGCTCCCATGCTCCAGGTAGCCTTCAGTTGAGGCAGAAGTGCGCATCTCAGTTCTTACCTACATCCGGGG CTGCTGCTTTGTCTGTGGAGACTTCCTCAGGTCCCTCCTCTTCCAACTCCTGCACTCAGTCTTCCCTGGC AGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302086
Insert Size	567 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302086.1, NP_001289015.1
RefSeq Size	1298 bp
RefSeq ORF	567 bp
Locus ID	22276
Gene Summary	The protein encoded by this gene is the fourth enzyme in the heme biosynthesis pathway. It converts hydroxymethylbilane to uroporphyrinogen III, a cyclic tetrapyrrole. This enzyme is defective in the autosomal recessive disorder congenital erythropoietic porphyria. Alternate promoter usage controls cell type-specific expression, including erythroid cell-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1, lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus compared to isoform 1.

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