Wnt11 (NM_001285795) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226147 representing NM_001285795 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGTCGGCCGCCACCATCAGTCACACCATCGCCCGGGCCTGCACCTCTGGCGACCTGCCCGGCTG CTCCTGCGGCCCCGTCCCAGGCTCTACGTGCCTCCCTGGAAACGAAGTGTAAATGCCATGGGGTGTCTGG CTCCTGCTCCATCCGCACCTGTTGGAAGGGGCTGCAAGAGCTCCAGGACGTGGCTGCTGACCTCAAGACC CGCTACCTGTCAGCCACGAAGGTGGTACACCGGCCTATGGGCACCCGCAAACACTTGGTGCCCAAGGACC TGGATATCCGGCCTGTGAAGGACTCAGAACTTGTGTATCTACAGAGCTCCCCTGACTTCTGCATGAAGAA TGAGAAGGTGGGATCCCATGGGACCCAAGACAGGCAGTGCAACAAGACTTCCAACGGCAGTGACAGCTGC GACCTCATGTGCTGTGGGCGCGGCTACAACCCCTACACGGACAGAGTGGTGGAGCGATGTCACTGCAAGT ACCACTGGTGCTGCTACGTCACCTGCCGCAGGTGTGAGCGCACGGTGGAGCGCTACGTCTGCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285795
Insert Size	558 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285795.1, NP_001272724.1
RefSeq Size	2624 bp
RefSeq ORF	558 bp
Locus ID	22411
Gene Summary	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate splice junction compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus compared to isoform 1.

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