Vps25 (NM_001284414) Mouse Untagged Clone
CAT#: MC225924
Vps25 (untagged) - Mouse vacuolar protein sorting 25 (yeast) (Vps25), transcript variant 4
CNY 2,000.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1110020N13Rik; AW107467; D11Wsu68e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC225924 representing NM_001284414
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTACCGCTTCCCGCCCTTCTTTACGTTACAGCCGAACGTGG ACACCCGGCAGAAGCAGCTGGCCGCCTGGTGCTCTCTGGTTCTGTCCTTCTGCCGCCTGCACAAACAGTC CAGCATGACGGTGATGGAAGCCCAGGAGAGCCCGCTTTTCAACAACGTCAAGCTACAGCGGAAACTTCCT GTGGAGTCAATTCAGATTGTATTAGAAGAACTGAGAAAGAAAGGGAACCTCGAGTGGTTGGATAAGAATA AGTCTAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATTTACCAATGGGTTTCCAG GAGTGGCCAGAATAACTCTGTGTTTACTCTGTATGAACTGACGAGTGGGGAAGACACAGAGGATGAAGCG AGCTCAAGATTTGTGGGTGTCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001284414 |
Insert Size | 447 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001284414.1, NP_001271343.1 |
RefSeq Size | 613 bp |
RefSeq ORF | 447 bp |
Locus ID | 28084 |
UniProt ID | Q9CQ80 |
Gene Summary | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 5' end of an exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR228135 | Vps25 (myc-DDK-tagged) - Mouse vacuolar protein sorting 25 (yeast) (Vps25), transcript variant 4 |
CNY 1,900.00 |
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