Cd83 (NM_001289915) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225898 representing NM_001289915 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCAAGGCCTCCAGCTCCTGTTTCTAGGCTGCGCCTGCAGCCTGGCACCCGCGATGGCGATGCGGG AGGTGACGGTGGCTTGCTCCGAGACCGCCGACTTGCCTTGCACAGCGCCCTGGGACCCGCAGCTCTCCTA TGCAGTGTCCTGGGCCAAGGTCTCCGAGAGTGGCACTGAGAGTGTGGAGCTCCCGGAGAGCAAGCAAAAC AGCTCCTTCGAGGCCCCCAGGAGAAGGGCCTATTCCCTGACGATCCAAAACACTACCATCTGCAGCTCGG GCACCTACAGGTGTGCCCTGCAGGAGCTCGGAGGGCAGCGCAACTTGAGCGGCACCGTGGTTCTGAAGGA TGCCCCAAGGAAGCTACAGAGTCAACTTTCAGGAAGTACAGGGCAGAAGCTGTGTTGCTCTTCTCTCTGG TTGTTTTCTACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289915
Insert Size	435 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289915.1, NP_001276844.1
RefSeq Size	2134 bp
RefSeq ORF	435 bp
Locus ID	12522
Gene Summary	May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, resulting in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.

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