Arhgdib (NM_001301309) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D4; Gdid; Gdid4; Ly-G; Ly-GDI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225720 representing NM_001301309 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTTACTGGCGATCTCGAGGCCCTCAAAAAGGATACATTTGTGCTAAAGGAAGGCATTGAATACA GGGTGAAAATTAACTTCAAAGTGAATAAGGATATTGTGTCTGGCCTGAAGTATGTTCAACACACATACCG GACTGGCATGAGAGTGGATAAAGCCACATTCATGGTTGGCAGCTATGGGCCCCGACCAGAGGAGTACGAA TTCCTCACTCCAGTAGAGGAAGCTCCCAAGGGCATGCTGGCCCGAGGCACTTACCACAACAAGTCCTTCT TCACGGATGACGACAAACAGGACCACCTCACCTGGGAATGGAACCTGGCCATTAAGAAGGATTGGACAGA ATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301309
Insert Size	354 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301309.1, NP_001288238.1
RefSeq Size	993 bp
RefSeq ORF	354 bp
Locus ID	11857
UniProt ID	Q61599
Gene Summary	The protein encoded by this gene is a member of the Rho guanine nucleotide dissociation inhibitor (GDI) family. This gene is expressed at high levels in hematopoietic cells. This protein is cytosolic, and dissociation of Rho from this protein is required for membrane association and activation of Rho by Guanine Nucleotide Exchange Factors (GEFs). C-terminal truncations of this gene product have been reported to promote metastasis. Multiple transcript variants and protein isoforms exist. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (6) lacks an in-frame exon in the coding region and uses a downstream start codon compared to variant 1. Variants 5 and 6 encode isoform 2 which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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