Unc80 (NM_175510) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C030018G13Rik; C230061B10Rik; UNC-80
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225476 representing NM_175510 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGAGGAAGAGCTCCGAGGGCCAGGAGCAGGACGGCGGCCGCGGCATCCCTCTGCCCATCCAGA CCTTCCTGTGGCGGCAAACCAGTGCATTTTTGAGGCCTAAACTGGGGAAGCAATATGAAGCCTCTTGTGT GTCCTTTGAACGAGTGTTGGTGGAAAACAAGCTGCATGGCCTGTCCCCGGCCCTCTCTGAAGCAATCCAG AGCATTTCCAGATGGGAGCTAGTGCAGGCTGCCCTCCCTCATGTTCTCCACTGCACTGCAACTCTGCTAT CAAACAGGAACAAACTAGGCCATCAAGATAAGTTGGGTGTTGCTGAGACAAAACTCCTTCATACCCTGCA CTGGATGCTTCTTGAGGCCCCACAGGACTGCAACAATGATCAGTTTGGAGGTACAGACCGAGGTTCCAGC TGGGGTGGCAGCAGCAGTGCTTTCATCCACCAGATTGAAAACCAGGGTTCTCCCGGGCAGCCTTGCCGAA GCAGCTCCCATGATGAGGAAGAGAACAACCGAAGGAAGACCTTCCAGAACTCCATGGCTACCGTGGAGCT CTTTGTGTTTCTGTTTGCTCCTTTGGTGCACAGGATAAAGGAATCTGATCTCACCTTCCGACTGGCCAGT GGGTTGGTTATATGGCAGCCTATGTGGGAGCACAGACAGCCTGAGGTCTCCGGCTTTACTGCGCTGGTAA AGCCCATAAGGAACATCATTACAGCCAAAAGAAGCTCTCCAATCAACAGTCAGAGTCAGACCTGTGAATC ACCCAATCAAGACACCAGGCAGCAAGGAGAGGGACTTCAGGTGGTCAGTGAAGCACTGCAGTCGGATTCC ATCTCACCTAAGGCCACCATCTCAGGCTGCCATCAGGGAAACTCCTTCGATGGAAGCCTGTCCTCCCAAA CGTCCCAGGAAAGAGGCCCGTCACATTCCAGGGCCTCACTTGTGATACCACCATGCCAGAGGTCACGCTA TGCCACCTACTTCGATGTTGCCGTTCTCCGCTGCCTTCTCCAGCCCCACTGGTCTGAGGAAGGCACTCAG TGGTCTCTGATGTACTATCTACAAAGGCTGCGACACATGCTGGAAGAGAAACCAGAAAAGACCCCAGATC CAGATATTCCTCTGCTGCCCAGGCCCAGAAGTAGCTCGATGGTGGCAGCAGCTCCCTCACTGGTGAACAC CCACAAGACTCAAGATCTCACCATGAAGTGTAATGAAGAAGAAAAATCTCTGAGCCCTGAGGCTTTTTCC AAAGTCTCATTGACCAATCTCCGAAGATCTGCCGTCCCAGATCTATCTTCAGATCTGGGCATGAACATTT TTAAAAAGTTCAAAAGTCGCAAAGAAGACCGAGAGAGGAAGGGCTCCATTCCATTCCACCACACGGGGAA GAGGAGACCTAGACGAATGGGTGTACCATTCCTGCTGCATGAGGACCACCTAGACGTCTCACCTACCCGC AGCACATTCTCTTTTGGCAGTTTCTCTGGGCTTGGAGAAGATAGGAGAGGCATTGAAAAGGGAGGCTGGC AAACCACCATCTTAGGGAAGCTGACCCGGCGGGGCAGCTCAGATGCAGCCACTGAGATGGAAAGTCTGAG CGCCAGGCACTCGCACTCCCACCACACCCTGGTGAGCGACCTGCCAGACCACTCCAACAGCCACGGAGAG AACACTGTCAAGGAAGTGCGATCTCAGATCTCCACCATCACAGTTGCGACCTTCAACACTACGCTGGCAT CATTCAATGTAGGCTACGCGGACTTCTTCAGTGAACACATGAGGAAGCTCTGCAGCCAGGTGCCTATCCC AGAGATGCCACATGAGCCTCTGGCATGTGCCAACCTGCCACGAAGCCTCACAGACTCCTGCATAAACTAC AGCTACCTGGAGGACACAGAACATATTGATGGCACGAACAACTTTGTCCATAAGAACGGCATGCTGGATC TCTCTGTGGTTCTGAAGGCTGTTTATCTCGTTCTCAACCATGACATCAGCTCTCGCATCTGTGATGTGGC ACTTAACATTGTGGAATGTTTGCTTCAACTTGGGGTAGTTCCCTGTGTAGAAAAAAACAGGAAGAAAAGT GAAAACAAGGAAAATGAGTCTGTGGAAAAGAGACCAAGTGAGGGAGCCTTCCAGTTCAAAGGCGTGTCTT CAAGTTCCACCAGTGGGTTTGGGGCCCCTTCCGCTAGTGGAGCTGGAGATGGTGGAGGAGAAGAAGGAGG AGGTGGAGATGGAGGAGGAGGAGGTGGAGGAGGAGATGGAGGAGGAGGTGGAGGAGGTGGAGGTGGCCCT TATGAGAAGAATGAGAAGAACCAAGAGAAGGATGACAACATCCCTGTAAGCAACCATAGGCTTGCTCTTA CTATGCTCATCAAAATAGTCAAGTCACTGGGATGTGCCTATGGTTGCGGAGAAGGACATCGAGGGCTCTC TGGAGATCGCCTGAGACACCAGGTATTCCGAGAGAATGCCCAGAACTGCCTTACTAAGCTGTACAAGCTA GATAAAATACAGTTCCGACAAACCATGAGGGACTATGTGAACAAGGACTCTCTCAATAATGTAGTGGACT TCTTGCATGCTTTGCTAGGATTTTGTATGGAGCCGGTCACTGACAACAAAGCTGGGTTTGGAAATAACTT CACCACAGTGGACAACAAGTCCACAGCCCAAAATGTGGAAGGCATTATTGTCGGTGCCATGTTCAAGTCT CTCATCACACGCTGTGCCTCAACCACACATGAACTCCACAGCCCTGAGAATCTGGGACTGTACTGTGACA TCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCT GCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTG GGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTC GAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACATGATGAACCAATGCAAGGGGGCAACCTGGG ACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAG CAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCC GCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGG CCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGC TCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGAC GAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCA AGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTC CTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAG CTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAA TTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCC ACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATG CAATTGGCATCCGATTGAATGAGCTGTGCCATGGAGAGAGCGAGAGTCCTGCCAACCTGCTCGGCCTTAT CTATGATGAGGAAACCAAGAGAAGACTGCGGAAGGAGGATGAGGAGGAAGACTTTTTAGATGACAGTACT GTGAACCCCTCTAAATGTGGCTGTCCCTTTGCCTTGAAGATGGCAGCATGCCAACTTCTCCTGGAGATAA CCACCTTCCTGAGGGAGACTTTTTCATGCCTGCCTAGACCTCGCACTGAGCCCCTCGTGGACTTGGAAAG CTGCAGGCTTCGTCTGGACCCTGAATTGGACCGGCACAGATATGAGAGGAAGATCAGCTTTGCAGGAGTT CTGGATGAAAATGAAGACTCAAAAGATTCTCTGCACAGCAGCAGCCACACCATCAAATCAGACGCAGGTG CTGAAGAGAAGAAAGTTCCCAGCAGGAAGATCAGGATAGGAGGTTCTCGACTGCTCCAGATTAAAGGAAC CCGCAGTTTCCAGGTGAAGAAGGGGGGTTCCTTGTCCAGCATTCGCCGAGTCGGCAGCTTAAAGAGCAGC AAGTTATCACGGCAGGACTCAGAGTCTGAGGCTGAGGAGCTGCAGCTGTCCCAGAGCAGGGACACTGTCA CTGACCTAGAAGGAAGTCCTTGGAGTGCAAGTGAACCCAGCATTGAGCCTGAGGGACTGAGCAATGCCGG CACAGAGGAAAACTACCACAGAAACATGTCGTGGCTTCACGTCATGATCTTACTCTGCAATCAACAAAGT TTCATTTGTACACATGTGGACTACTGCCATCCACACTGCTACCTGCACCACAGCCGCTCCTGTGCCCGGC TGGTCAGGGCCATTAAATTGCTGTATGGGGACAGTGTGGACTCCCTGCGTGAGAGCAACCACATCAGCAA CGTGGCTCTGAGGGGCAAGAAACAGAAAGAGTGCTCAGATAAGTCATGCCTGAGGACACCTTCCCTGAAG AAGAGAGTTTCGGATGTCAACCTGGAAGGAAAAAAGGACTCGGGAATGCTGAAGTACATCAGGTTTCAGG TGATGAGCTTGTCACCTGCTCCCTTGTCTCTGCTCATCAAGGCTGCTCCGATTCTGACAGAGGAGATGTA TGGAGACATCCAGCCAGCTGCCTGGGAACTGCTACTCAGTATGGATGAGCACATGGCTGGGGCAGCAGCC GCCATGTTCCTGTTGTGTGCTGTGAAGGTCCCCGATGCTGTGTCAGACATGCTGATGTCCGAGTTCCATC ATGCAGAGACTGTACAGAGGCTGAATGCTGTCCTCAAGTTTCACACTCTCTGGAGGTTTCGCTATCAGGT CTGGCCTAGGATGGAGGAGGGAGCACAGCAGATTTTTAAGATCCCGCCTCCCAGCATAAACTTTACCCTG CCATCACCAGTACTTGGAATGCCATCTGTCCCAATGTTCGACCCTCCGTGGGTCCCTCAGTGCAGCGGGA GTGTCCAGGACCCCATTAACGAAGACCAGTCTAAATCCTTCTCTGCCCGGGCAGTGTCCCGCTCCCATCA GAGGGCAGAGCACATCTTAAAGAACTTGCAGCAGGAGGAAGAGAAGAAACGCCTTGGTCGTGAGGCGAGC CTCATCACCGCCATCCCCATCACCCAAGAGGCTTGCTATGAGCCCACCTGCACACCCAACTCAGAGCCTG AGGAAGAAGAAGAAGTCGCCAATCTGACATCCCGACGGCTGTCTGTGAGTCCATCCTGCACCTCCAGTAC GTCGCACAGGAATTATTCCTTCCGCAGAGGGTCAGTCTGGTCCGTGCGTTCAGCAGTCAGTGCTGAAGAT GAGGAGCACGCCACTGAGCACACCCCGAACCACCATGTGCCTCAGCCTCCGCAAGCAGTGTTCCCAGCAT GCATCTGTGCCGCAGTTCTTCCCATTGTTCATTTAATGGAGGATGGCGAGGTGCGGGAAGATGGCGTGGC AGTCAGTGCTGTGGCCCAGCAAGTCTTGTGGAACTGTCTAATTGAAGATCCATCAACAGTTCTTCGACAT TTTCTGGAAAAACTGACCATCAGCAATAGACAAGATGAGCTAATGTACATGCTCCGAAAACTTCTCTTAA ATATTGGAGACTTTCCAGCTCAAACATCACACATCCTGTTCAACTACCTGGTGGGGTTAATCATGTACTT TGTGAGGACCCCGTGTGAGTGGGGTATGGATGCCATCTCTGCCACGCTGACCTTCCTGTGGGAGGTGGTC GGATATGTGGAGGGCCTCTTCTTCAAGGATCTCAAGCAGACGATGAAGAAGGAGCAATGCGAAGTGAAGC TGCTGGTCACTGCTTCCATGCCAGGTACCAAAACCTTGGTAGTCCATGGGCAGAATGAGTGTGACATCCC AACACAGCTGCCAGTACATGAAGACACTCAGTTTGAAGCCCTGCTGAAGGAGTGTCTGGAGTTTTTCAAC ATCCCAGAATCCCAGTCAACGCATTATTTTCTCATGGATAAACGATGGAACCTCATCCACTATAACAAGA CCTATGTTCGAGACATTTATCCCTTCCGGAGGTCCGTCTCGCCACAGCTGAATCTCGTCCACATGCATCC GGAGAAGGGGCAGGAGCTCATTCAGAAGCAGGTGTTCACCCGGAAGCTGGAGGAAGTAGGGCGGGTTTTG TTCCTCATCTCCCTTACCCAGAAGATCCCCACGGCCCACAAGCAGTCACATGTCTCCATGCTCCAGGAGG ACCTTCTGCGGCTGCCCTCCTTCCCGCGGAGTGCAATAGACGCTGAGTTTTCTCTCTTCAGTGACCCGCA AGCTGGGAAGGAACTGTTTGGCCTCGACACCCTGCAGAAAAGCTTGTGGATCCAACTCCTGGAGGAGATG TTCCTGGGCATGCCGAGCGAGTTTCCCTGGGGAGACGAAATCATGCTTTTTCTCAATGTGTTCAATGGGG CTCTGATTCTCCACCCAGAAGACAGTGCCCTGCTCAGGCAGTACGCAGCCACTGTCATCAACACAGCTGT CCACTTCAATCACCTGTTCTCTCTTAGTGGCTACCAGTGGATTCTCCCCACCATGCTGCAGGTATACTCA GACTATGAAAGCAACCCCCAGCTGCGTCGCGCCATCGAATTTGCCTGCCACCAGTTCTACATCCTACACA GAAAGCCCTTTGTGCTCCAACTGTTTGCTAGTGTGGCCCCTCTTCTGGAGTTCCCGGATGCTGCCAATAC AGGGTCCAGCAAAGGTGTGTCTGCTCAGTGCCTGTTTGACTTGCTGCAGTCTCTGGAGGGAGAAACCACT GACATATTGGACATTCTAGAGCTGGTTAAAGCTGAGAAGCCCCTTAAGTCACTAGATTTCTGCTATGGGA ATGAAGACCTGACATTCTCCATCAGTGAAGCCATCAAGCTCTGCGTCACCGTGGTGGCTTACGCTCCTGA GTCATTCCGAAGCCTTCAGATGCTAATGGTCTTGGAGGCTTTAGTTCCATGCTACCTACAGAAGATGAAG AGGCAGACGTCCCAGGTAGAGACAGTGCCTGCTGCCCGGGAGGAGATTGCTGCCACAGCTGCTCTGGCCA CATCACTACAAGCCCTTTTGTACAGTGTGGAGGTCCTCACCAGGCCCATGACAGCCCCACAGATGAGCAG GAGTGACCAAGGTCACAAGGGAACCACCACAGCCAACCATACCATGTCGTCTGGAGTGAACACCAGGTAC CCGGAGCAAGGAGCCAAGCTGCACTTTATCAGGGAAAACCTCCATTTACTAGAGGAAGGGCAAGGCCTTC CCAGAGAAGAACTAGATGAACGAATTTCTCGAGAAGAATTCAGAAGACCCCGGGAATCTCTGCTGAACAT TTGCACGGAATTCTATAAGCACTGCGGGCCGAGGCTGAAGATCCTGCAAAACCTAGCTGGGGAGCCACGG GTCACTGCCCTGGAGCTGCTGGATGTGAAGTCTCACATGAGGCTGGCAGAAATTGCACACTCCCTCCTGA AGCTGGCACCATATGACACCCAGACAATGGAGAGCCGAGGCCTTCGGCGCTACATCATGGAGATGCTCCC CATTACTGATTGGTCAGCAGAGGCTGTGAGGCCAGCTCTTATCCTCATTCTAAAGAGACTGGATAGAATG TTCAACAAAATCCATAAGATGCCCACCTTGAGGCGACAGGTTGAATGGGAGCCTGCCAGCAGTTTGATTG AAGGGGTTTGTTTGACACTTCAGAGGCAGCCAATCATATCCTTCCTGCCTCACCTTAGGTCACTGATCAA TGTCTGTGTCAATCTGGTGATGGGAGTGGTAGGACCTTCCAGTGTTGCGGATGGATTACCCCTTCTTCAT CTCAGCCCTTATCTCTCACCACCTCTGCCCTTCAGCACAGCTGTTGTCCGGCTTGTAGCATTGCAGATAC AGGCTTTAAAAGAAGATTTTCCTTTAAGCCATGTGATCTCCCCATTCACCAATCAAGAGCGAAGGGAGGG GATGCTTTTAAATCTGCTCATCCCATTTGTGCTCACAGTAGGATCTGGAAGCAAAGATAGCCCGTGGCTG GAGCAGCCCGAGGTACAGCTACTGCTGCAGACTGTCATTAATGTGCTCCTGCCCCCTCGGATCATCAGCA CATCCAGGAGCAAGAACTTCATGCTGGAGAGCTCCCCAGCACACTGCTCTACCCCCGGGGATGCAGGCAA AGACTTGCGCAAGGAAGGGCTGGCTGAGTCCACCAGCCAGGCAGCATATTTAGCTCTCAAGGTTATTCTC GTCTGCTTCGAGAGGCAGCTGGGCAGCCAGTGGTACTGGCTGAGCCTGCAGGTGAAGGAGATGGCTCTGC GAAAGGTGGGCGGCCTGGCCCTGTGGGACTTCCTCGACTTCATCGTGCGCACCCGGATACCCATCTTCGT TCTCTTGCGCCCTTTCATCCAATGCAAGCTATTGGCCCAACCAGCAGAAAATCACGAGGAGCTTTCTGCC CGGCAACACATCTCCGACCAGCTGGAGCGGCGCTTCATCCCCCGCCCTTTATGCAAGAGCTCTCTCATTG CAGAGTTCAATAGCGAGCTGAAAATTCTGAAAGAGGCAGTCCACAGTGGATCGGCCTACCAAGGAAAGAC ATCTATCAGCACTGTAGGCACATCCACTTCTGCCTACCGCCTGAGCCTGGCCACCATGTCTCGCTCCAAC ACAGGCACAGGCACTGTCTGGGAGCAGGACAGCGAACCATCCCAACAGGCTTCCCAAGACACCCTCAGTC GGACCGATGAGGAGGACGAGGAAAATGACTCTGTAAGCATGCCCAGTGTGGTAAGTGAACAGGAAGCTTG TCTCCTCAGCACCATTGGAAGGAGGCGGTTCTCCAGCCACGTCTCCAGCATGTCTGCACCTCAGGCTGAG GTGGGCATGTTACCCAGTCAAAGTGAACCTAATGTCCTCGATGACTCCCAGGGCCTGGCCGCCGAGGGCA GCCTCTCTAGGGTGGCAAGTATACAGAGTGAACCTGGACAGCAGAACGTCCTCCTACAGCAGCCACTGGG GAGGAAGAGGGGCCTGAGGCAGCTAAGACGCCCTCTCCTGTCAAGGCAGAAGACTCAAACGGAACCCAGA AACCGCCATGGAGCTCGACTGTCCACCACTCGCAGGAGCATTCAACCTAAAACAAAACCATCTGTTGATC AGAAGCGATCTGTGACATTCATTGAGGCTCAGCCAGAGCCAACAGCTGCCCCAACAGACATATTTCCTGC CACGGGCCAACCACAGAGCTGCAGCCCAGGCCGAGCTAGGAAGCCAGAAGGAACAGAGAAGCCAGTGCTC ACATCCTCCCCCGCCATTATCATTGCAGATCTTCATAGCCTGTCTCCCAAGCAGAGTGAGCCCCTTCTTG CTGAAGAAGGAGAGAAAAAGGAGGACGAAGAAATACAAGGTGCCACAGCTCACTGCCCCCTCTCCACTCA ACTGTCAGACCCGGATGACTTCACTGGCCTTGAAACATCCAGCCTCCTACAGCATGGAGACACTGTCCTT CATATCAGTGAGGAGAACGGCACGGAGAATCCCCTGCTGTCCAGCCAGTTCACTTTCACTCCTCCCGAGC TGGGAGACACAGACTCAGCACTAGATGAGTCCCACGTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175510
Insert Size	9981 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175510.3, NP_780719.2
RefSeq Size	13533 bp
RefSeq ORF	9981 bp
Locus ID	329178
UniProt ID	Q8BLN6
Gene Summary	Component of the NALCN sodium channel complex, required for channel regulation. This complex is a cation channel activated by neuropeptides substance P, neurotensin, and extracellular calcium that regulates neuronal excitability by controlling the sizes of NALCN-dependent sodium-leak current. UNC80 is essential for NALCN sensitivity to extracellular calcium.[UniProtKB/Swiss-Prot Function]

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