Frem2 (NM_172862) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6030440P17Rik; 8430406N05Rik; b2b1562Clo; Gm409; my; ne; nv1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225465 representing NM_172862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCGTGCGCGCAGGACAGCCAAGTTCTCCAGCTTCCAGCCGATACTCGCACAGTCGCCTAGGC TGCTGCTGCTGCTGCTGCTCCTGTCACTGGTAAGCTACGTCTCCACACAGGCTGCTGGCCCCGGTGCAGC ACTTCAGTCCCTGGGTCTCTCCGGGACTTCGGGAGTCCCTACAGAGGAAGCTATAGTGGTGGCAAACCGC GGGCTCCGTGTGCCCTTCGGTCGCGAAGTCTGGCTGGATCCGTTGCGTGACTTGGTGCTGCAGGTGCAGC CAGGAGACCGCTGCACAGTGACCGTGTTAGACAACGACGCGCTGGCCCAGCGGCCGGGCCACTTGAGTCC CAAGCGTTTTGCGTGCGACTATGGCCCGGGTGAGGTGCGCTACTCGCACCTGGGTGCTCGCAGCCCTTCC CGCGACCGCGTCCGCCTGCAGCTGCGCTACGATGCTCCAGGAGGGGCGATAGTCCTGCCACTGGCACTAG AGGTGGAAGTGGTCTTCACCCAGCTGGAGATTGTGACTCGGAATTTACCTCTGGTCGTGGAAGAACTGCT AGGGACCAGTAATGCCTTGGACGATCGGAGCTTAGAGTTCGCCTACCAGCCTGAGACCGAGGAGTGCCGC GTGGGCATCTTGTCAGGTCTGAGCGCTTTGCCTCGTTACGGAGAACTCCTTCACTACCCACAGGTCCAGG GAGGGGCCGGAGATAGAGGGACCTCTAAGACCCTTTTGATGGACTGCAAAGCATTCCAGGAGCTGGGAGT CCGCTACCGCCACACAGCCCCCAGCCGCTCGCCTAACAGGGACTGGCTGCCTATGGTGGTCGAGCTGCAT TCGCGAGGGGCTCCAGAGGGCAGCCCGGCTCTAAAACGCGAACACTTCCAGGTGCTTGTGAGGATTCGAG GAGGAGCGGAGAACACTGCACCCAAGCCCAGTTTCGTGGCCATGATGATGATGGAGGTGGACCAGTTTGT ACTGACTGCTCTCACTCCAGACATGCTAGCAGCCGAGGATGCTGAATCCGACCCAGACCTTTTGATTTTC AACCTCACCTCTGCCTTCCAGCCTGGGCAGGGCTACCTGGTGAGCACGGATGACAGAAGCCTGCCTCTCT CCTCCTTTACACAGCGGGATTTGCGCCTGCTGAAGATTGCCTACCAGCCTCCCTCTGAAGACTCGGATCA GGAGCGTCTGTTTGAACTAGAGCTGGAGATAGTGGACCCTGAAGGAGCAGCCTCAGACCCTTTCGCCTTC ATGGTGGTGGTGAAACCCATGAACACACTGGCTCCCGTGGTCACACGAAACACTGGCCTTATTCTCTATG AGGGTCAATATCGGCCGCTCACGGGTCCCATAGGCAGTGGACCCCAGAACTTGGTCATCAGTGATGAAGA TGACCTAGAAGCTGTGAGGCTGGAGGTGGTGGCTGGGCTCCGGCATGGCCACCTTGTTATTCTGGGTTCT CCCAGTAGCGACTCTGCTCCCAAGACCTTCACAGTGGCGGAGCTGGCGGCTGGCCAGGTTGTGTATCAGC ATGATGACAAAGACGGTTCACTGAGTGACAACTTGGTGCTGCGCATGTCCGATGGAGGAGGCAGGCACCA GGTCCAGTTCCTGTTCCCTATCACTTTAGTGCCTGTGGATGACCAGCCGCCGGTCCTGAACGCCAACACT GGGCTGACAGTGGCTGAGGGGGAAACTGTGCCTATCCCACCCTTGACTCTCAGTGCAACCGATATAGATT CAGATGACTCTCAGCTGGTTTTTGTCCTGTTGCCACCCTTCTCAAGCTTGGGGCACCTGCTTCTCCGCCA AAGGCATGTCCCCCAGGAAGAGCAGGGTCTGTGGCAGAAGCAAGGGTCATTTTACGAGCGAACGGTGACA GAGTGGCGGCAACAGGATATAACAGAGGGGAAGCTCTTCTATAGGCACTCGGGGCCCCACAGCCCTGGGC CGGTGATGGACCAGTTCATGTTTAGAGTCCAAGACAATCATGATCCCCCTAACCAGTCTGGGATCCAGAG GTTTGTAATACGCATTCATCCCGTGGACCGTCTCCCTCCAGAACTGGGCAGTGGCTGTCCCCTTCGGATG GTGGTACAGGAATCCCAGCTCACACCACTGAGGAAAAGGTGGCTGCACTACACTGACCTGGACACAGATG ACCGAGAGTTACAGTACACAGTGACCCAGCCTCCCACGGACACAGATGAGAACCACTCGCCGGCTCCACT GGGCACGTTGGTCTTCACTGATAATCCCTCAGTGGTGGTGAGCCATTTTACCCAAGCCCAGGTCAATCAT CATAAAATTGCTTACAGGCCCCCAGGGCAGGAGCTAGGAGTCGCAGCCCGAGTGGCTCAGTTCCAGTTCC AAGTAGAGGATCGAGCTGGGAATGTGGCTCCTGGCACCTTCACCCTTTACCTGCAGCCTGTGGATAACCA GCCCCCCGAGATTGTCAACACTGGCTTCACCGTTGAGGAGAAGGGCCACCACATCCTGCGTGAGACCGAG CTGCATGTGAGTGATGTTGACACGGATGTGACCCACATCTCGTTCACTCTCACACAGGCCCCCAAACACG GCCACATGCAAATATCTGGACGGCCACTGCATGTAGGCGGACAATTCCACTTGGAGGACATAAAGCATGG CAGAATTTCCTACTGGAATAGCGGGGATGAGTCTTTGACTGACAGCTGCTCCCTGGAGGTCAGCGACAGA CACCATGTGGTGCCAATCACTCTCAGAGTGAACGTTCGGCCAGGGGACCGTGAAGGTCCTATGTCAGTCC TTCCTGCTGGTACTCTGGAGTCCTATCTAGACGTCTTAGAGAATGGGGCTACTGAAGTCACTGCCAATAT CATTAAGGGGGCCTATCAGGGTACCGATGATTTGATGCTGACCTTCCTGTTAGAAGGCCCACCCTCCTAT GGGGAAATCTTAGTCAACGGAGCTCCAGCTGAGCAGTTCACCCAGAGGGACATCCTGGAGGGCTCTGTTG TCTACGCTCACACCAGTGGTGAGATTGGCCTGTTGCCCAAAGCTGACTCTTTTAACCTGAGTCTATCAGC TATGTCTCAAGAATGGAGAATCGGTAGCAGCATCGTTCAAGGCGTCACTGTGTGGGTGACCATCCTCCCC GTGGACAGCCAAGCCCCTGAGATTTCTCTTGGTGAACAGTTTGTAGTGCTGGAAGGTGACAAGAGTGTGA TAAGCCTGACCCACCTAAGTGCTGAGGACATGGACTCCCTGAAGGATGACCTCTTGTGCACAATAGTCAT TCAGCCAACCTCAGGCTATGTAGAGAACATTTCTCCAGCACCAGGCTCAGAGAAATCAAGAGCCGGGGTT GCCATAAGTGCCTTCACGCTGAAGGATCTCAGGCAAGGACACATTAACTATGTCCAGAGTGTCCACAGAG GAGTGGAGCCTGTCGAAGACCGCTTTATATTCCGTTGTTCTGACGGTATTAACTTCTCTGAGAGACAGAT ATTCCCCATTGTCATCATTCCTACCAATGACGAGCAGCCAGAAATGTTCATGAGGGAATTCATGGTGATG GAAGGCATGAGTCTGGTGGTCAACAGACTCATCCTCAACGCTGCTGATGCCGACATTCCCAGGGATGATC TAACGTTTACTATTACCCGGTTTCCAACTCACGGTCATGTCATGAACCAGTTAATCAATGGTACAGTGTT GGTTGAAAGCTTCACCCTGGATCAGATTATAGAGAGTTCCAGCATTATTTATGAGCACGATGACTCTGAG ACACAAGAGGACAGTTTTGTGATTAAACTGACAGATGGCAAGCACTCCGTGGAGAAGATGGTCCTCATTG TGGTCATCCCTGTTGATGACGAGACACCCAGAATGACCATCAATAATGGGCTAGAAATAGAAATTGGGGA AACCAAAGTTATCAACAACAAAGTCCTAATGGCAACCGATCTAGACTCTGATGACAAATCACTGGTTTAT ATTATCCGTTATGGACCAGGGCATGGCTTGTTACAGAGACAAAAACCTCTAGGTGCCTTTGAAAATATCA CTCTAGGCATGAATTTTACCCAGGACGAAGTGGACAGGAACTTGATTCAATATGTCCACTTTGGGCAAGA AGGTATTCGAGACCTGATTAAATTCGATGTGACAGATGGAACAAATGCTCTTATAGATCGCTACTTTTAC GTGACTATTGGAAGTGTTGACATTGTCTTCCCTGATGTGGTAAGTAAGGGCGTGTCCTTGAAAGAAGGTG GCAAAGTCACTCTGACAACGGATCTGCTGAGCACAAGTGACTTGAACAGCCCTGATGAGAACTTAGTTTT CACCATTACCAGGGCTCCTATGAGAGGTCACTTGGAGTGCACGGATCGAAGGGGACTGTCCATCACTTCT TTCACTCAGCTCCAGCTGGCTGGTAACAAAATCTACTACATACACACGGCTGAGGATGAGGTCAAAATGG ACAGCTTCGAGTTTCAAGTCACCGATGGGCGGAACCCTGTCTTCCGAACTTTCCGGATCTCAATCAGTGA CGTAGACAACAAAAAGCCAGTGGTCACCATCCACAACCTGGTGGTCAGTGAAAGTGAAAGTAAGCTGATC ACCCCCTTTGAACTCACTGTGGAGGACAGAGATACCCCTGACAGGCTCCTAAAATTCATTGTCACCCAGG TCCCTGTTCACGGTCACCTCCTCTTCAACAATACCAGATCTGTCATGGTTTTCACCAAGCAAGACTTGAA TGAAAACTTAATCAGCTACAAACACGATGGCACAGAATCCACGGAAGACAGCTTCTCCTTCACCGTGACT GACGGCACCCACTCGGACTTCTATGTTTTTCCTGATACAGTGTTTGAAACCAGGAGACCTCAAGTGATGA AAATCCAGGTGTTACCTGTTGACAACAGTGTCCCCCAAATTGTGGTGAACAAAGGAGCTTCTACCCTTCG TACCCTGGCCACTGGCCATTTAGGGTTCATGATCACAAGCAAAATACTCAAAGTGGAGGACAGAGACAGC CTGCATTTTTCTCTCAGGTTCATTGTGACAGAAGCCCCCCAACATGGCTACCTTCTCAACCTGGGCCAAG GAAACCACAGTGTCACACAGTTCACCCAAGCTGACATTGATGACATGAAAATATGCTATGTCTTGAGAGA GAGAGCAAATGCCACAAGTGATATGTTCCATTTCATAGTGGAAGATGACGGTGGAAACAGGTTAACCAAC CAGCATTTTCGACTGAATTGGGCGTGGATCTCCTTTGAAAAAGAGTATTACTTGATCAACGAAGACTCCA AATTTCTAGATATTGTTCTTACACGCAGAGGATACTTGGGAGAAACTTCATTTATAAGTATCGGCACCAG GGATGGGACTGCGGAAAAAGACAGAGACTTCAAGGGTAAAGCTCAGAAACAGGTACAGTTCAACCCCGGC CAGACCAGGGCCTCGTGGAGAGTGCGCATCCTGAGCGACGGGGAACACGAGCACTCGGAGACCTTCCAAG TGGTTCTCTCGGAGCCTGTGCTGGCAATCCTGGAGTTCCCCACAGTGACTACAGTGGAGATCATCGACCC AGGAGACGAATCAACCGTGTTCATTCCCCAGTCTGAGTACTCTGTTGAAGAAGATGTGGGTGAGCTGTTC ATTCCCATCAGGAGAAGTGGAGACATCAGCCGGGAGCTGATGGTGATCTGCTACACACAGCAAGGGACAG CCACCAGCACTGTGCGGACGTCCGTGCTCTCTTACTCTGACTACATCTCCAGGCCCGAGGACCACAGCAG CGTGATCCGCTTTGACAAAGACGAACGAGAGAAAATGTGTCGTATTTTGGTAATTGATGACTCTTTGTAC GAAGAGGAGGAGACTTTCCAGGTCCTTTTGAGCATGCCCATGGGCGGGAGGATCGGTGACAAGTTTCCAG GAGCCAATGTGACCATCCTCACAGACAGGGATGATGAGCCTGCCTTCTACTTTGGGGACACCCAGTACTC CGTGGATGAGAGTGCCGGCTACGTGGAGCTGCAGGTGTGGAGGACGGGGACCGACCTCTCTAAGCCTTCC AGTGTCACAGTGAGGTCCCGGAAAACAGAATCCCTCTCTGCAGATGCTGGAACAGACTATGTGGGAATCA GTCGCAATTTAGATTTTGCGCCTGGCGTCAACATGCAGACTGTTCGAGTGGTCATTCTGGATGACCTTGG ACGACCAATACTGGAGGGAATTGAGAAGTTTGAACTGGTACTTCGAATGCCTATGAACGCTGCCCTTGGG GAGCCCAGCAAGGCCACCGTGTCCATAAATGACTCTGCCTCAGACTTGCCTAAAATGCAATTCAAAGAGA GAGTGTACACTTGCAACGAGAATGACGGGCGTGTAGTGGCCATGATCTACAGGAGCGGTGACATCCAGCA CAGGTCTTCTGTGAGATGTTACACGAGGCAGGGGTCTGCGCAGGTGATGATGGACTTCGAGGAACGCCCA AATACCGATGTTTCCACTGTCACATTCCTCCCTGGTGAGATGGAGAAGCCGTGTGTCCTCGAGCTGATGG ATGATGCTGTCTATGAGGATGTAGAGGAGCTGCGCCTGGTACTTGGCACGCCTCAGGGCAGCTCTGCCTT TGGAGCTGCAGTGGGCGAGCAGAACGAAACCCTGATAAAGATCCAGGATGAGGCTGACAAAGCTGTTATC AAATTTGGAGAAACCAAATTTAGTGTCACTGAGCCCAGCAGACCAGGAGAGTCGGTGGTTGTCAAAATCC CGGTCATCCGTCAAGGAGACACTTCAAAGGTCTCCATCGTGAGAGTCCACACCAAGGATGGCTCGGCTAC CTCCGGAGAGGATTATCACCCTGTGTCAGAAGAAATCGAGTTTAAGGAGGGAGAAACCCAACACACTGTT GAAATTGAAGTAATCTTCGACGGGGTGAGAGAGATGAGGGAGGCCTTCACTGTTCACCTAAAACCAGATG AAAATATGGTGGCAGAGACACAGGCAACCAAGGCCATTGTATATATAGAAGAAATCCACAGCATGGCAGA TGTCACCTTTCCTTCTGTCCCTCACATTGTGTCCTTGCTGATATACGATGATCCCTCCAAAGGCAGAGAG GACACTGGACCTGTGTCTGGCTACCCGGTTGTCTGCATTACGGCCTGCAACCCCAAATATCCAGACTATG AGAAAACAGGCTCTATTTGTGCCAGTGAGAACATCAACGACACTTTGACCCGATACAGATGGCTGATCAG CGCTCCCGCTGGCCCTGACGGTGTGACCAGCCCCATGAGAGAGGTCGACTTCGACACCTTCTTCACATCA TCCAAAATGATTACATTAGACTCCATATACTTTCAGCCTGGCTCCCGGGTCCAATGTGCGGCCCGAGCAG TGAACACCAATGGGAATGAAGGCCTGGAACTAATGAGCCCCATCGTTACCATCGGCAGAGAGGAAGGTCT CTGTCAGCCCCGTGTGCCTGGGGTAGTGGGGGCAGAGCCATTCTCCGCTAAGTTGCGCTACACTGGCCCA GAGGACCCAGACTTTGCCAACCTCATCAAACTCACTGTCACTATGCCGCACATAGACGGCATGCTCCCTG CCATCTCCACAAGAGAGCTGTCCAACTTTGAGCTGACCCTCAGCCCTGACGGCACGAGAGTTGGGAATCA CAAATGCTCCAACCTGCTGGACTACAATGAAGTAAAGACCCACCATGGCTTCCTGACCAACGCCACAAAG AACCCAGAGGTCATTGGAGAGACCTATCCTTACCAGTACAGTGTGCCGGTCAGGGGTTCCAGCACCTTAC GCTTCTACCGAAACCTGAACCTGGAAGCATGTCTGTGGGAGTTTGTCAGCTACTATGACATGACGGAGCT CCTGGCTGACTGTGGAGGAACCATCGGGACCGATGGCCAGGTCCTCAACCTCGTGCAGTCCTACGTGACC CTTCGAGTTCCTCTGTACGTTTCCTACGTGTTCCACTCCCCCGTGGGCGTGGGCGGCTGGCAGCATTTCG ACTTGAAGTCAGAGCTCCGACTCACATTCGTGTACGACACTGCCATCTTGTGGAACCATGGGATCGGCAG CCCCCCGGAAGCTGAGCTGCAAGGCTCTCTTTACCCAACCAGCATGCGCATTGGTGAGGAGGGACGCCTG GCTGTGAACTTCAAAACAGAGGCTCAGTTCCATGGCTTGTTTGTGCTGTCCCATCCTGCTTCCTTTACCA GCTCACTGATCGTGTCAGCTGACCATCCGGGCCTCACATTTTCCCTCCGCCTTATACGGAGTGAACCAAC CTACAATCAGCCAGTCCAGCAGTGGAGTTTCGTGTCAGACTTTGCGGTCCGTGACTACTCGGGCACCTAC ACAGTAAAGTTGGTACCCTGCACCACTCCATCCAACCAGGAGTACCGTCTGCCAGTCACCTGCAACCCCA GAGAGCCTGTCACCTTTGACCTTGACATTCGATTCCAACAGGTCAGCGATCCCGTGGCGACTGAGTTCAG CCTGAACACCCACATGTATCTGCTGTCTAAGAAGAATCTCTGGTTGTCTGATGGGTCCATGGGATTTGGG CAGGAAAGCGACGTGGCTTTTGCAGAAGGGGATGTGATTTATGGCCGTGTCATGGTCGATCCTGTCCAGA ACCTCGGTGACTCCTTTTACTGCAGCATTGAGAAGGTGTTCTTATGCACGGGGGATGACGGCTACGTGCC CAAGTACAGCCCAGCGAATGCGGAATATGGCTGCCTGGCTGATTCTCCTTCACTCTTACATAGATTCAAA ATCGTGGATAAGGCTCAGCCAGAGACACAAGCCACCAGCTTTGGAGATGTCCTGTTTAACGCCAAACTGG CAGTGGATGACCCTGAAGCTGTTCTCTTAGTGAATCAGCCTGGATCCGATGGTTTTAAAGTAGACTCAAC ACCACTCTTTCAGGTTGCCTTGGGCCGAGAGTGGTACATACACACAATCTATACGGTAAAGTCTAAGGAC AACACCCATCGAGGCATTGGCAAGAGGAGCCTGGAGTACCAGTATCACTCAGTGGTGCATCCCGGACCAC CGCAGGCAACCACCAAGAGCTGGAAGAAGAGAGCAGTCAGGAGCACGCCCTCCCTCGCAGGGGAGATCGG TGCTGAGAACAACCGGGGAACAAACATCCAACATATCTCCCTGAACCGCCGTGGCAAGAGGCAGGTCCCC CACGGAAGAATTCCTCCTGACGGCATCCTGCCCTGGGAGCTCAATAGTCCCAGCTCTGAAGTGAGCCTTG TCACTGTCCTGGGAGGCCTCACGGTGGGCTTGCTCACAGTCTGCCTGGCTGTGGCTGCGGCCGTCATGTG CAGGAACAGGAGCACTAAGGGCAAGGATACTCCAAAGGGCTCTGGAAGCACTGAGCCCATGATGTCCCCG CAGAGCCACTACAACGACAGCTCGGAGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172862
Insert Size	9483 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172862.3, NP_766450.2
RefSeq Size	12368 bp
RefSeq ORF	9483 bp
Locus ID	242022
UniProt ID	Q6NVD0
Gene Summary	Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. May be required for epidermal adhesion.[UniProtKB/Swiss-Prot Function]

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