Syne1 (NM_001079686) Mouse Untagged Clone

CAT#: MC225445

Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 3, (10ug)



  "NM_001079686" in other vectors (2)

CNY 48,360.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225445 representing NM_001079686
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCCTCAGGAGCTGGCGCAGAAAATCAAGGGCTACCAGGAGCAGATCGACTCTTTGAATTCCAAAT
GCAAGATGCTGACGATGAAAGCCAAGCACGCAACTATGCTGCTGACTGTGACCGAGGTCGAGGGACTGGC
TGAAGGGACCGAGGATCTGGACCGCGAGCTCCACCCTACGCCTTCAGCCCACCCCTCTGTGGTCATGATG
ACTGCAGGTCGCTGTCATACTCTGCTGTCACCTGTCACTGAAGAGTCTGGGGAAGAGGGAACCAACAGTG
AGATTTCCTCTCCACCTGCCTGTCGCTCCCCATCACCTGTGGCTAACACGGAGGCGGCTGTTAACCAGGA
CATTGCGTACTACCAAGCTCTGTCAGCCGAGGGGTTGCAGACAGATGCTGCAAGAATTCCCCCCAGTGCT
GCTGTATCCCAAGAGTTGTATGAGCCAGGACTGGAGCCTTCAGCGACTGCCAAGCTGGGTGACTTACAAC
GTTCCTGGGAAACCTTAAAAAACGTGATCAGTGAGAAACAGCGGACTCTCTACGAAGTTTTGGAACGCCA
GCAGAAGTACCAAGACTCTCTCCAATCCATCTCGACTAAAATGGAGGCCATGGAGATGAAGCTAGGTGAG
AGCCTCGAGCCCAGCCGGAGTCCAGAGAGCCAGATGGCTGAGCACCAGGCCCTGATGGACGAGGTGCAGA
TGCTACAAGACGAGATCAATGGGCTCCAGGTGTCTCTTGCTGAGGAGCTGGTGGCCGAGTCTCAGGAGTC
GGACCCCGCGGAGCAGCTAGCTCTGCAGTCCACGCTCACTGTCCTGGCTGAGAGAATGTCCACCATCAGG
ATGAAAGCAGCTGGCAAGCGGCAGCTTCTGGAGGAGAAGCTAAGTGATCAGCTGGAGGAGCAGAGACAGG
AGCAGGCTCTGCAGAGGTACCGCTGCGAGGCAGATGAGCTGGACCACTGGCTGCTGAACACTAAGGCTAC
CCTGGATGTTGCACTGGGTACATCCCAGGAGCCCATGGACATGGATGCCCAGCTGGTGGACTGCCAGAAC
ATGCTGGTGGAAATCGAACAGAAAGTGGTGGCCCTGTCGCAGCTGTCCGTGCACAATGAGAACCTGCTGT
TGGAGGGCAAGGCTCACACTAAAGAGGAGGCCGAGCAGCTAGCGGTGAAGCTGAGGCTACTCAAAGGGAG
CCTTGGAGAGCTGCAGAGGGCCCTGCATGACAGACAGCTCGACATGCAGGGAGTCACACAAGAGAAAGAG
GAGAACGATGTTGACTTCACAGACACCCAGAGCCCCGGCGTGCAGGAATGGCTGGCACAAGCCCGCACCA
CAAGGACCCACCAGCGGCAGAGCAGCCTCCAGCAGCAAAAAGAGTTCGAACAGGAGCTGGCCGAGCAGAA
GAGCCTCCTCCGCTCAGTAGCTAGCCGTGGAGAGGAGATTCTAACCCAACATTCCACCGCAGAAGGTTCA
GGTGGCCTTGGTGAAAAACCTGATGTGTTATCCCAGGAGTTGGGGATTGCTGAAGACCAGATGAGAGTGA
AGTGGGAAAGCCTACACCAAGAGTTTAGTGCCAAGCAGAAGCTACTACAGAATATTCTGGAACAGGAGCA
AGAACAAGTGCTTTACAGTAGTCCGAACAGACTCCTGTCAGGAGTACTTCCATTCAGAGGGGAAGCACAG
ACTCAGGATAAGACTTCAGTGACGTCCTTGCTGGATGGGCTGAGCCAGGCCTTCGGGGAGGCGTCATCAC
AGAGTGGAGGGACAGACAGACAGAGCATTCACTTGGAGCAGAAGCTATATGACGGAGTCTCAGCCACTTC
CACCTGGTTGAATGACGTCGAGGAGCGCTTATTTGTAGCCACGGCACCTTTGCCAGAAGAAACAGAAGCT
TGTCTCTTCAACCAGGAGGCTCTTGCCAAGGACATTAAGGAAATGTCTGAAGAAATGGATAAGAACAAGA
ACTTGTTTTCCCAAGCATTCCCAGAGGACAGTGACAACCGGGATGTCATTGAAGATACTCTGGGTTGTCT
TTTGGGCAGATTGTCCCTGCTGGATTCAGTAGTGGATCAGCGGTGTCATCAGATGAAGGAAAGACTCCAG
CAAATCCTCCGTTTCCAGAATGACCTGAAGGTGTTGTTTACATCGTTAGCTGACAGCAAATATATCATTC
TGCAGAAGCTGGCAAATGTGTTTGAACAGCCCATTGTGGAACAGATGCAGGCCATCCAACAGGCTGAAGA
GGGACTCAGGGACCTCGAGGGTGGAATCTCTGAGTTGAAACGGTGGGCTGACAAGTTGCAAGTGGAACAG
TCTGCTGTGCAGGAACTCTCCAAGCTCCAGGACATGTATGACGAGCTATTGATGACTGTCAGCTCCCGGA
GGAGCAGCCTCCACCAGAACCTTGCTCTCAAGAGTCAGTATGATAAGGCTCTGCAAGATCTGGTGGACCT
GCTGGACACTGGACAGGAGAAGATGACAGGGGACCAAAAGATCATTGTATGCTCCAAAGAAGAAATCCAG
CAGCTGCTTGGGAAGCACAAGGAATACTTCCAGGGCCTGGAATCGCACATGATTTTGACTGAAATACTCT
TCAGGAAGATCGTTGGCTTTGCAGCTGTGAAGGAAACTCAGTTCCATACGGACTGCATGGCTCAGGCCTC
TGCTGTGTTGAAGCAGGCTCACAAGAGAGGGGTGGAGCTTGAGTACATCCTAGAGATGTGGTCCCATCTG
GATGAGAATCGCCAGGAGCTCAGCAGACAGCTGGAGGTGATAGAAAACAGCATCCCAAGTGTGGGCCTGG
TAGAAGAGAGTGAGGACAGACTTGTGGAAAGGACAAATCTCTACCAGCATTTAAAATCCAGCCTCAATGA
ATACCAGCCCAAACTGTACCAAGCACTGGATGATGGGAAGCGACTTCTGATGTCTGTCAGCTGTTCAGAG
CTTGAAAGTCAGCTAAATCAGCTGGGAGAGCACTGGCTCAGTAATACGAACAAAGTATCCAAGGAGCTGC
ACAGACTGGAGACCATACTGAAACACTGGACCAGATATCAAAGTGAAGCTGCAGCTCTCAACCACTGGCT
ACAGTGCGCTAAGGACAGGCTGGCATTTTGGACTCAGCAGTCTGTGACGGTTCCACAAGAACTGGAAATG
GTCCGGGATCATCTCAGTGCTTTCCTGGAGTTCTCTAAAGAAGTAGATGCCAAGTCTGCCCTCAAGTCGT
CAGTCACAAGCACAGGAAATCAGCTGCTTCGGTTAAAAAAAGTGGACACAGCGGCCCTGCGTGCTGAGCT
GTCCCGCATGGACAGCCAGTGGACTGACCTACTGACCGGCATCCCTGTTGTACAGGAGAAGCTCCATCAG
CTCCAGATGGACAAGCTGCCTTCCCGCCATGCCATTTCTGAGGTAATGAGTTGGATCTCACTCATGGAAA
GTGTTATCCTGAAGGATGAAGAGGACATTAGAAATGCCATCGGTTACAAGGCGATCCACGAATACCTTCA
GAAATACAAGGGTTTCAAGATAGACCTTAACTGCAAGCAGCTAACGGCGGACTTTGTGAACCAGTCAGTG
CTCCAAATCAGCAGTCAGGACGTGGAGAGCAAGCGCAGTGACAAGACGGATTTTGCTGAGCAGCTTGGGG
CAATGAATAAAAGTTGGCAGCTCTTGCAAGGTCGAGTTGGAGAGAAGATCCAGATGTTGGAAGGCTTGTT
GGAGTCATGGTCAGAGTATGAAAACAGTGTCCAGTCTCTGAAAGCCTGGTTTGCAAACCAGGAGAGGAAG
CTGAAAGAGCAGCATCTACTTGGCGATCGGAATTCTGTTGAGAATGCTCTGAAGGACTGTCAGGAACTGG
AAGATTTGATTAAAGCAAAAGAAAAGGAGGTAGAGAAAATTGAGCAGAATGGCCTCGCATTGATTCAGAA
CAAGAGGGAGGAGGTCTCTGGCAGTGTCATGAGCACACTGCAAGAACTCAGGCAGACCTGGATCAGTTTA
GACCGCACGGTTGAACAATTAAAGATCCAGCTGACCTCAGCCCTTGGCCAGTGGAGCAATCACAAAGCAG
CCTGTGACGAGATCAACGGTCACCTCATGGAGGCCAGATACTCCCTGTCCCGATTCCGTCTGTTGACAGG
TTCCTCCGAAGCTGTACAAGTTCAGGTCGACAATCTTCAGAATCTTCATGATGAACTAGAAAAACAGGAA
GGAGGCTTACAGAAATTTGGCTCTATCACCAACCAACTATTGAAGGAGTGTCACCCACCTGTGGCAGAAA
CCCTCTCCAGTACTCTGCAAGAAGTCAATATGAGATGGAATAACTTACTGGAAGAGATCGCTGAACAGCT
GCACTCCAGCAAGGCCCTCCTTCAGCTCTGGCAGAGATACAAGGACTACTCAAAGCAGTGTGCCTCTGCC
ATCCAGAGACAAGAGGAACAGACCAGTGTACTGTTGAAGGCAGCCACTAACAAGGATATCGCTGATGATG
AAGTCACCAAGTGGATTCAGGATTGCAACGACCTTCTCAAGGGACTGGAGACAGTCAAGGACTCGCTTTT
CATCCTCCGTGAACTAGGAGAGCAGCTTGGACAGCAGGTGGATGTTTCCGCAGCAGCAGCCATTCAGTGT
GAGCAGCTCTGTTTCAGCCAGCGCCTGGGGGCCCTGGAGCAGGCTCTTTGCAAGCAGCAAGCAGTATTGC
AGGCTGGAGTTGTTGACTATGAAACATTTGCCAAGAGCTTAGAAGCCTTAGAGGTCTGGATGGTGGAAGC
CGAGGGCATCCTCCAAGGACAGGATCCCACCCATTCCTCTGACCTCTCTACTATCCAGGAAAGGATGGAG
GAACTGAAGGGACAGATGCTAAAGTTCAGCAGCTTGGCTCCTGATTTAGACCGTCTCAATGAGTTGGGGT
ACCGGTTACCCTTAAATGACAAGGAGATCAAGAGGATGCAGAACCTGAACCGCCATTGGTCTCTGACCTC
TTCTCAGACCACAGAAAGATTCAGCAAGTTGCAGTCATTCTTGCTTCAGCACCAGACTTTCTTGGAAAAG
TGTGAGACATGGATGGAATTTCTGGTTCAGACAGAGCACAAGTTGGCAGTGGAGATCTCAGGCAATTACC
AGCATCTTTTGGAGCAGCAAAGAGCACATGAGCTTTTTCAAGCCGAGATGTTCAGCCGCCAGCAGATCCT
CCACTCCATCATTGTTGATGGGCAGAATCTTCTAGAACAAGGTCAAGTTGATGATAGGGAAGAGTTCAGC
CTGAAGTTGACGCTCCTCAGTAACCAGTGGCAGGGAGTGATTCGAAGGGCCCAACAGAGAAGGGGGATCA
TTGACAGCCAGATTCGCCAGTGGCAACGCTATAGGGAAATGGCAGAGAAGCTCCGTAAATGGTTGGCTGA
GGTGTCTCATCTGCCCCTGAGTGGTCTCGGGAACATCCCAGTACCACTGCAACAAGTGAGGATGCTCTTT
GATGAAGTTCAGTTCAAAGAGAAGGTGTTCCTGCGCCAGCAAGGGAGCTACATCCTGACAGTGGAGGCTG
GCAAGCAGCTCCTGCTCTCTGCCGACAGTGGCGCCGAGGCCGCCCTGCAGGCTGAGCTCACAGACATCCA
AGAGAAGTGGAAGGCAGCCAGCATGCATCTGGAGGAGCAGAAGAAAAAGCTGGCTTTTCTGTTGAAAGAC
TGGGAAAAATGTGAGAGAGGGATAGCCAATTCTCTGGAGAAGCTCCGAATGTTCAAAAAGAGGTTATCTC
AGCCTCTACCTGACCACCATGAGGAACTTCATGCAGAGCAGATGCGGTGTAAGGAACTGGAAAACGCAGT
TGGGAGGTGGACAGATGACCTGACAGAGTTGATGCTGGTGAGGGATGCCCTGGCTGTCTACCTCAGTGCT
GAGGACATCTCCATGCTGAAGGAACGCGTGGAGCTTCTGCAAAGACAATGGGAAGAGCTTTGTCACCAGG
TCTCCCTACGACGACAGCAAGTAAGCGAGCGCCTGAATGAGTGGGCTGTCTTCAGTGAAAAGAACAAGGA
GCTGTGTGAGTGGCTGACCCAGATGGAGAGCAAAGTCTCTCAGAACGGAGACATTCTCATCGAAGAGATG
ATAGAAAAACTCAAGAAGGATTATCAAGAGGAAATTGCTGTTGCCCAAGAGAACAAAATCCAGCTTCAGG
AAATGGGAGAACGGCTTGCTAAAGCCAGCCACGAGAGCAAAGCCTCTGAGATTCAGTACAAGCTCAGCAG
AGTGAAGGACAGGTGGCAGCATCTCCTGGACCTCATGGCAGCTAGGGTGAAGAAGCTAAAGGAGACGCTG
GTTGCTGTGCAGCAGCTGGACAAGAACATGGGCAGCCTGAGGACTTGGCTGGCCCACATGGAGTCAGAGT
TGGCCAAGCCCATAGTCTATGATTCTTGTAACTCTGAAGAAATACAGAGGAAACTGAATGAGCAGCAGGA
GCTCCAGAGAGACATCGAGAAACACAGCACAGGTGTCGCCTCCGTCCTCAACCTGTGTGAGGTTCTGCTG
CATGACTGCGACGCCTGTGCCACCGACGCAGAGTGTGACTCCATCCAGCAGGCCACTCGCAACCTGGACC
GGCGGTGGAGGAACATCTGCGCCATGTCCATGGAGAGGAGACTCAAAATTGAAGAGACGTGGCGACTGTG
GCAGAAGTTTCTGGATGACTATTCTCGCTTTGAAGACTGGCTGGAGGTTTCAGAAAGGACGGCTGCTTTC
CCCAGTTCTTCCGGGGTGCTCTATACAGTTGCCAAGGAGGAGCTGAAGAAGTTTGAGGCTTTCCAGCGGC
AGGTCCATGAGAGCCTGACCCAACTAGAGCTTATCAACAAACAGTACCGGCGCCTGGCCAGAGAGAATCG
CACAGACTCCGCCTGCAGCCTCAGGCAGATGGTCCATGGGGGCAACCAGAGATGGGACGACCTACAGAAG
CGGGTCACCTCCATCTTGCGCAGGCTCAAGCACTTCATTAGCCAGCGTGAGGAGTTCGAGACTGCCCGGG
ACAGCATTCTTGTCTGGCTCACAGAGATGGATCTGCAGCTCACCAATATTGAACACTTTTCGGAATGTGA
CGTTCAAGCTAAAATAAAGCAACTCAAGGCTTTCCAACAGGAGATCTCACTGAACCACAATAAAATCGAG
CAGATCATTGCCCAGGGGGAGCAGCTGATAGAGAAGAGCGAGCCACTGGATGCCGCTGTCATCGAGGAGG
AACTGGACGAGCTCAGACGCTACTGCCAGGAGGTCTTTGGACGTGTGGAAAGATATCACAAGAAACTGAT
CCGCCTGCCTCTGCCAGATGACCATGACCTCTCAGACCGAGAACTAGATCTCGAAGACTCCACGGCTCTT
TCAGACCTCCGCTGGCAGGACCCGTCTGCAGATGGCATGCCCTCCCCACAGCCTTCTTCCAACCCCTCCC
TCTCCCTTCCTCAGCCCCTCCGGAGTGAGCGGTCAGGGCGAGATACGCCGGCCAGCGTGGACTCCATCCC
CCTGGAATGGGACCACGATTACGACCTCAGTCGAGACCTAGAGTCAGCATCCCGAACTCTGCCCTCAGAA
GATGAAGAAGGCGAGGAAGACAAGGAGTTCTACCTCAGGGGAGCTGTTGGCTTGTCAGGGGATCCCAGCT
CACTGGAGTCACAGATGAGGCAACTGGACAAAGCCCTGGATGACAGCCGCTTCCAGATACAGCAAACCGC
AAATATCCTCAGAAGCAAAACCCCCACAGGGCCAGACCTGGACACCAGCTACAAGGGCTACATGAAGCTG
CTGGGCGAGTGCAGTGGCAGCATAGATTCCGTGAGGAGACTGGAGCACAAGCTGGCGGAGGAAGAGAGCT
TTCCTGGCTTCGTTAACCTCAACAGCACAGAGACTCAGACAGCTGGTGTAATTGACCGCTGGGAGCTCCT
GCAGGCCCAGGCAATGAGCAAGGAGTTGAGGATGAAGCAGAACCTTCAGAAGTGGCAGCAGTTTAATTCT
GACCTCAACAACATCTGGGCCTGGCTAGGAGAGACAGAGGAGGAGCTGGACAGGCTCCAGCACCTGGCGC
TCAGCACTGACATCCACACCATCGAGTCTCACATCAAAAAGCTCAAGGAGCTCCAGAAAGCCGTGGACCA
CCGCAAAGCCATCATCCTCTCCATCAACCTCTGCAGCTCTGAGTTCACCCAGGCTGACAGCAAAGAGAGT
CATGACCTGCAGGATCGCTTATCCCAGATGAATGGGAGATGGGATCGAGTGTGCTCTCTGCTGGAGGACT
GGAGAGGCTTGCTGCAGGATGCACTGATGCAGTGCCAGGAGTTCCATGAAATGAGCCATGCCTTGCTTCT
CATGCTAGAAAACATTGACAGAAGGAAAAATGAAATTGTCCCAATTGATTCAACCCTTGACCCAGAGACA
CTTCAGGACCATCACAAGCAACTCATGCAAATAAAGCAGGAGTTGCTGAAGTCCCAACTCAGAGTGGCCT
CACTACAAGACATGTCTCGCCAACTCTTGGTGAATGCTGAAGGTTCAGATTGTCTAGAAGCCAAAGAAAA
GGTCCATGTGATTGGAAACCGGCTCAAACTTCTCCTGAAGGAGGTCAGCCATCATATCAAGGATCTTGAG
AAGTTACTAGACATGTCAAGCAGTCAGCAGGATTTGTCATCATGGTCTTCTGCAGATGAGCTGGACACCT
CAGGATCTGTGAGTCCCACATCCGGAAGAAGTACCCCAAACAGACAGAAATCGCCACGAGGCAAATGTAG
TCTCTCACAGCCTGGACCCTCTGTCAGCAGCCCAAAGAGCAGGTCCACAAGAGATGGCTCCGATTCCTCC
CGTTCTGACCCCAGGCCAGAACGGGTGGGTCGAGCCTTCCTGTTCCGGATCCTCCGGGCAGCTCTTCCCT
TTCAGCTGCTCCTGCTGCTGCTTATTGGACTCACCTGCCTTGTACCCATGTCAGAGAAAGACTACAGCTG
TGCCCTCTCCAACAACTTTGCCCGATCCTTCCATCCGATGCTCAGATATACCAACGGTCCTCCTCCACTC
TGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001079686
Insert Size 9033 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001079686.1, NP_001073154.1
RefSeq Size 10876 bp
RefSeq ORF 9033 bp
Locus ID 64009
UniProt ID Q6ZWR6
Gene Summary Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and lacks a large portion of the 5' coding region found in variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (3) is shorter and has distinct N-terminus compared to isoform 4.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...