Syne1 (NM_001079686) Mouse Untagged Clone
CAT#: MC225445
Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 3, (10ug)
CNY 48,360.00
货期*
12周
规格
Product images
经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC225445 representing NM_001079686
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCCTCAGGAGCTGGCGCAGAAAATCAAGGGCTACCAGGAGCAGATCGACTCTTTGAATTCCAAAT GCAAGATGCTGACGATGAAAGCCAAGCACGCAACTATGCTGCTGACTGTGACCGAGGTCGAGGGACTGGC TGAAGGGACCGAGGATCTGGACCGCGAGCTCCACCCTACGCCTTCAGCCCACCCCTCTGTGGTCATGATG ACTGCAGGTCGCTGTCATACTCTGCTGTCACCTGTCACTGAAGAGTCTGGGGAAGAGGGAACCAACAGTG AGATTTCCTCTCCACCTGCCTGTCGCTCCCCATCACCTGTGGCTAACACGGAGGCGGCTGTTAACCAGGA CATTGCGTACTACCAAGCTCTGTCAGCCGAGGGGTTGCAGACAGATGCTGCAAGAATTCCCCCCAGTGCT GCTGTATCCCAAGAGTTGTATGAGCCAGGACTGGAGCCTTCAGCGACTGCCAAGCTGGGTGACTTACAAC GTTCCTGGGAAACCTTAAAAAACGTGATCAGTGAGAAACAGCGGACTCTCTACGAAGTTTTGGAACGCCA GCAGAAGTACCAAGACTCTCTCCAATCCATCTCGACTAAAATGGAGGCCATGGAGATGAAGCTAGGTGAG AGCCTCGAGCCCAGCCGGAGTCCAGAGAGCCAGATGGCTGAGCACCAGGCCCTGATGGACGAGGTGCAGA TGCTACAAGACGAGATCAATGGGCTCCAGGTGTCTCTTGCTGAGGAGCTGGTGGCCGAGTCTCAGGAGTC GGACCCCGCGGAGCAGCTAGCTCTGCAGTCCACGCTCACTGTCCTGGCTGAGAGAATGTCCACCATCAGG ATGAAAGCAGCTGGCAAGCGGCAGCTTCTGGAGGAGAAGCTAAGTGATCAGCTGGAGGAGCAGAGACAGG AGCAGGCTCTGCAGAGGTACCGCTGCGAGGCAGATGAGCTGGACCACTGGCTGCTGAACACTAAGGCTAC CCTGGATGTTGCACTGGGTACATCCCAGGAGCCCATGGACATGGATGCCCAGCTGGTGGACTGCCAGAAC ATGCTGGTGGAAATCGAACAGAAAGTGGTGGCCCTGTCGCAGCTGTCCGTGCACAATGAGAACCTGCTGT TGGAGGGCAAGGCTCACACTAAAGAGGAGGCCGAGCAGCTAGCGGTGAAGCTGAGGCTACTCAAAGGGAG CCTTGGAGAGCTGCAGAGGGCCCTGCATGACAGACAGCTCGACATGCAGGGAGTCACACAAGAGAAAGAG GAGAACGATGTTGACTTCACAGACACCCAGAGCCCCGGCGTGCAGGAATGGCTGGCACAAGCCCGCACCA CAAGGACCCACCAGCGGCAGAGCAGCCTCCAGCAGCAAAAAGAGTTCGAACAGGAGCTGGCCGAGCAGAA GAGCCTCCTCCGCTCAGTAGCTAGCCGTGGAGAGGAGATTCTAACCCAACATTCCACCGCAGAAGGTTCA GGTGGCCTTGGTGAAAAACCTGATGTGTTATCCCAGGAGTTGGGGATTGCTGAAGACCAGATGAGAGTGA AGTGGGAAAGCCTACACCAAGAGTTTAGTGCCAAGCAGAAGCTACTACAGAATATTCTGGAACAGGAGCA AGAACAAGTGCTTTACAGTAGTCCGAACAGACTCCTGTCAGGAGTACTTCCATTCAGAGGGGAAGCACAG ACTCAGGATAAGACTTCAGTGACGTCCTTGCTGGATGGGCTGAGCCAGGCCTTCGGGGAGGCGTCATCAC AGAGTGGAGGGACAGACAGACAGAGCATTCACTTGGAGCAGAAGCTATATGACGGAGTCTCAGCCACTTC CACCTGGTTGAATGACGTCGAGGAGCGCTTATTTGTAGCCACGGCACCTTTGCCAGAAGAAACAGAAGCT TGTCTCTTCAACCAGGAGGCTCTTGCCAAGGACATTAAGGAAATGTCTGAAGAAATGGATAAGAACAAGA ACTTGTTTTCCCAAGCATTCCCAGAGGACAGTGACAACCGGGATGTCATTGAAGATACTCTGGGTTGTCT TTTGGGCAGATTGTCCCTGCTGGATTCAGTAGTGGATCAGCGGTGTCATCAGATGAAGGAAAGACTCCAG CAAATCCTCCGTTTCCAGAATGACCTGAAGGTGTTGTTTACATCGTTAGCTGACAGCAAATATATCATTC TGCAGAAGCTGGCAAATGTGTTTGAACAGCCCATTGTGGAACAGATGCAGGCCATCCAACAGGCTGAAGA GGGACTCAGGGACCTCGAGGGTGGAATCTCTGAGTTGAAACGGTGGGCTGACAAGTTGCAAGTGGAACAG TCTGCTGTGCAGGAACTCTCCAAGCTCCAGGACATGTATGACGAGCTATTGATGACTGTCAGCTCCCGGA GGAGCAGCCTCCACCAGAACCTTGCTCTCAAGAGTCAGTATGATAAGGCTCTGCAAGATCTGGTGGACCT GCTGGACACTGGACAGGAGAAGATGACAGGGGACCAAAAGATCATTGTATGCTCCAAAGAAGAAATCCAG CAGCTGCTTGGGAAGCACAAGGAATACTTCCAGGGCCTGGAATCGCACATGATTTTGACTGAAATACTCT TCAGGAAGATCGTTGGCTTTGCAGCTGTGAAGGAAACTCAGTTCCATACGGACTGCATGGCTCAGGCCTC TGCTGTGTTGAAGCAGGCTCACAAGAGAGGGGTGGAGCTTGAGTACATCCTAGAGATGTGGTCCCATCTG GATGAGAATCGCCAGGAGCTCAGCAGACAGCTGGAGGTGATAGAAAACAGCATCCCAAGTGTGGGCCTGG TAGAAGAGAGTGAGGACAGACTTGTGGAAAGGACAAATCTCTACCAGCATTTAAAATCCAGCCTCAATGA ATACCAGCCCAAACTGTACCAAGCACTGGATGATGGGAAGCGACTTCTGATGTCTGTCAGCTGTTCAGAG CTTGAAAGTCAGCTAAATCAGCTGGGAGAGCACTGGCTCAGTAATACGAACAAAGTATCCAAGGAGCTGC ACAGACTGGAGACCATACTGAAACACTGGACCAGATATCAAAGTGAAGCTGCAGCTCTCAACCACTGGCT ACAGTGCGCTAAGGACAGGCTGGCATTTTGGACTCAGCAGTCTGTGACGGTTCCACAAGAACTGGAAATG GTCCGGGATCATCTCAGTGCTTTCCTGGAGTTCTCTAAAGAAGTAGATGCCAAGTCTGCCCTCAAGTCGT CAGTCACAAGCACAGGAAATCAGCTGCTTCGGTTAAAAAAAGTGGACACAGCGGCCCTGCGTGCTGAGCT GTCCCGCATGGACAGCCAGTGGACTGACCTACTGACCGGCATCCCTGTTGTACAGGAGAAGCTCCATCAG CTCCAGATGGACAAGCTGCCTTCCCGCCATGCCATTTCTGAGGTAATGAGTTGGATCTCACTCATGGAAA GTGTTATCCTGAAGGATGAAGAGGACATTAGAAATGCCATCGGTTACAAGGCGATCCACGAATACCTTCA GAAATACAAGGGTTTCAAGATAGACCTTAACTGCAAGCAGCTAACGGCGGACTTTGTGAACCAGTCAGTG CTCCAAATCAGCAGTCAGGACGTGGAGAGCAAGCGCAGTGACAAGACGGATTTTGCTGAGCAGCTTGGGG CAATGAATAAAAGTTGGCAGCTCTTGCAAGGTCGAGTTGGAGAGAAGATCCAGATGTTGGAAGGCTTGTT GGAGTCATGGTCAGAGTATGAAAACAGTGTCCAGTCTCTGAAAGCCTGGTTTGCAAACCAGGAGAGGAAG CTGAAAGAGCAGCATCTACTTGGCGATCGGAATTCTGTTGAGAATGCTCTGAAGGACTGTCAGGAACTGG AAGATTTGATTAAAGCAAAAGAAAAGGAGGTAGAGAAAATTGAGCAGAATGGCCTCGCATTGATTCAGAA CAAGAGGGAGGAGGTCTCTGGCAGTGTCATGAGCACACTGCAAGAACTCAGGCAGACCTGGATCAGTTTA GACCGCACGGTTGAACAATTAAAGATCCAGCTGACCTCAGCCCTTGGCCAGTGGAGCAATCACAAAGCAG CCTGTGACGAGATCAACGGTCACCTCATGGAGGCCAGATACTCCCTGTCCCGATTCCGTCTGTTGACAGG TTCCTCCGAAGCTGTACAAGTTCAGGTCGACAATCTTCAGAATCTTCATGATGAACTAGAAAAACAGGAA GGAGGCTTACAGAAATTTGGCTCTATCACCAACCAACTATTGAAGGAGTGTCACCCACCTGTGGCAGAAA CCCTCTCCAGTACTCTGCAAGAAGTCAATATGAGATGGAATAACTTACTGGAAGAGATCGCTGAACAGCT GCACTCCAGCAAGGCCCTCCTTCAGCTCTGGCAGAGATACAAGGACTACTCAAAGCAGTGTGCCTCTGCC ATCCAGAGACAAGAGGAACAGACCAGTGTACTGTTGAAGGCAGCCACTAACAAGGATATCGCTGATGATG AAGTCACCAAGTGGATTCAGGATTGCAACGACCTTCTCAAGGGACTGGAGACAGTCAAGGACTCGCTTTT CATCCTCCGTGAACTAGGAGAGCAGCTTGGACAGCAGGTGGATGTTTCCGCAGCAGCAGCCATTCAGTGT GAGCAGCTCTGTTTCAGCCAGCGCCTGGGGGCCCTGGAGCAGGCTCTTTGCAAGCAGCAAGCAGTATTGC AGGCTGGAGTTGTTGACTATGAAACATTTGCCAAGAGCTTAGAAGCCTTAGAGGTCTGGATGGTGGAAGC CGAGGGCATCCTCCAAGGACAGGATCCCACCCATTCCTCTGACCTCTCTACTATCCAGGAAAGGATGGAG GAACTGAAGGGACAGATGCTAAAGTTCAGCAGCTTGGCTCCTGATTTAGACCGTCTCAATGAGTTGGGGT ACCGGTTACCCTTAAATGACAAGGAGATCAAGAGGATGCAGAACCTGAACCGCCATTGGTCTCTGACCTC TTCTCAGACCACAGAAAGATTCAGCAAGTTGCAGTCATTCTTGCTTCAGCACCAGACTTTCTTGGAAAAG TGTGAGACATGGATGGAATTTCTGGTTCAGACAGAGCACAAGTTGGCAGTGGAGATCTCAGGCAATTACC AGCATCTTTTGGAGCAGCAAAGAGCACATGAGCTTTTTCAAGCCGAGATGTTCAGCCGCCAGCAGATCCT CCACTCCATCATTGTTGATGGGCAGAATCTTCTAGAACAAGGTCAAGTTGATGATAGGGAAGAGTTCAGC CTGAAGTTGACGCTCCTCAGTAACCAGTGGCAGGGAGTGATTCGAAGGGCCCAACAGAGAAGGGGGATCA TTGACAGCCAGATTCGCCAGTGGCAACGCTATAGGGAAATGGCAGAGAAGCTCCGTAAATGGTTGGCTGA GGTGTCTCATCTGCCCCTGAGTGGTCTCGGGAACATCCCAGTACCACTGCAACAAGTGAGGATGCTCTTT GATGAAGTTCAGTTCAAAGAGAAGGTGTTCCTGCGCCAGCAAGGGAGCTACATCCTGACAGTGGAGGCTG GCAAGCAGCTCCTGCTCTCTGCCGACAGTGGCGCCGAGGCCGCCCTGCAGGCTGAGCTCACAGACATCCA AGAGAAGTGGAAGGCAGCCAGCATGCATCTGGAGGAGCAGAAGAAAAAGCTGGCTTTTCTGTTGAAAGAC TGGGAAAAATGTGAGAGAGGGATAGCCAATTCTCTGGAGAAGCTCCGAATGTTCAAAAAGAGGTTATCTC AGCCTCTACCTGACCACCATGAGGAACTTCATGCAGAGCAGATGCGGTGTAAGGAACTGGAAAACGCAGT TGGGAGGTGGACAGATGACCTGACAGAGTTGATGCTGGTGAGGGATGCCCTGGCTGTCTACCTCAGTGCT GAGGACATCTCCATGCTGAAGGAACGCGTGGAGCTTCTGCAAAGACAATGGGAAGAGCTTTGTCACCAGG TCTCCCTACGACGACAGCAAGTAAGCGAGCGCCTGAATGAGTGGGCTGTCTTCAGTGAAAAGAACAAGGA GCTGTGTGAGTGGCTGACCCAGATGGAGAGCAAAGTCTCTCAGAACGGAGACATTCTCATCGAAGAGATG ATAGAAAAACTCAAGAAGGATTATCAAGAGGAAATTGCTGTTGCCCAAGAGAACAAAATCCAGCTTCAGG AAATGGGAGAACGGCTTGCTAAAGCCAGCCACGAGAGCAAAGCCTCTGAGATTCAGTACAAGCTCAGCAG AGTGAAGGACAGGTGGCAGCATCTCCTGGACCTCATGGCAGCTAGGGTGAAGAAGCTAAAGGAGACGCTG GTTGCTGTGCAGCAGCTGGACAAGAACATGGGCAGCCTGAGGACTTGGCTGGCCCACATGGAGTCAGAGT TGGCCAAGCCCATAGTCTATGATTCTTGTAACTCTGAAGAAATACAGAGGAAACTGAATGAGCAGCAGGA GCTCCAGAGAGACATCGAGAAACACAGCACAGGTGTCGCCTCCGTCCTCAACCTGTGTGAGGTTCTGCTG CATGACTGCGACGCCTGTGCCACCGACGCAGAGTGTGACTCCATCCAGCAGGCCACTCGCAACCTGGACC GGCGGTGGAGGAACATCTGCGCCATGTCCATGGAGAGGAGACTCAAAATTGAAGAGACGTGGCGACTGTG GCAGAAGTTTCTGGATGACTATTCTCGCTTTGAAGACTGGCTGGAGGTTTCAGAAAGGACGGCTGCTTTC CCCAGTTCTTCCGGGGTGCTCTATACAGTTGCCAAGGAGGAGCTGAAGAAGTTTGAGGCTTTCCAGCGGC AGGTCCATGAGAGCCTGACCCAACTAGAGCTTATCAACAAACAGTACCGGCGCCTGGCCAGAGAGAATCG CACAGACTCCGCCTGCAGCCTCAGGCAGATGGTCCATGGGGGCAACCAGAGATGGGACGACCTACAGAAG CGGGTCACCTCCATCTTGCGCAGGCTCAAGCACTTCATTAGCCAGCGTGAGGAGTTCGAGACTGCCCGGG ACAGCATTCTTGTCTGGCTCACAGAGATGGATCTGCAGCTCACCAATATTGAACACTTTTCGGAATGTGA CGTTCAAGCTAAAATAAAGCAACTCAAGGCTTTCCAACAGGAGATCTCACTGAACCACAATAAAATCGAG CAGATCATTGCCCAGGGGGAGCAGCTGATAGAGAAGAGCGAGCCACTGGATGCCGCTGTCATCGAGGAGG AACTGGACGAGCTCAGACGCTACTGCCAGGAGGTCTTTGGACGTGTGGAAAGATATCACAAGAAACTGAT CCGCCTGCCTCTGCCAGATGACCATGACCTCTCAGACCGAGAACTAGATCTCGAAGACTCCACGGCTCTT TCAGACCTCCGCTGGCAGGACCCGTCTGCAGATGGCATGCCCTCCCCACAGCCTTCTTCCAACCCCTCCC TCTCCCTTCCTCAGCCCCTCCGGAGTGAGCGGTCAGGGCGAGATACGCCGGCCAGCGTGGACTCCATCCC CCTGGAATGGGACCACGATTACGACCTCAGTCGAGACCTAGAGTCAGCATCCCGAACTCTGCCCTCAGAA GATGAAGAAGGCGAGGAAGACAAGGAGTTCTACCTCAGGGGAGCTGTTGGCTTGTCAGGGGATCCCAGCT CACTGGAGTCACAGATGAGGCAACTGGACAAAGCCCTGGATGACAGCCGCTTCCAGATACAGCAAACCGC AAATATCCTCAGAAGCAAAACCCCCACAGGGCCAGACCTGGACACCAGCTACAAGGGCTACATGAAGCTG CTGGGCGAGTGCAGTGGCAGCATAGATTCCGTGAGGAGACTGGAGCACAAGCTGGCGGAGGAAGAGAGCT TTCCTGGCTTCGTTAACCTCAACAGCACAGAGACTCAGACAGCTGGTGTAATTGACCGCTGGGAGCTCCT GCAGGCCCAGGCAATGAGCAAGGAGTTGAGGATGAAGCAGAACCTTCAGAAGTGGCAGCAGTTTAATTCT GACCTCAACAACATCTGGGCCTGGCTAGGAGAGACAGAGGAGGAGCTGGACAGGCTCCAGCACCTGGCGC TCAGCACTGACATCCACACCATCGAGTCTCACATCAAAAAGCTCAAGGAGCTCCAGAAAGCCGTGGACCA CCGCAAAGCCATCATCCTCTCCATCAACCTCTGCAGCTCTGAGTTCACCCAGGCTGACAGCAAAGAGAGT CATGACCTGCAGGATCGCTTATCCCAGATGAATGGGAGATGGGATCGAGTGTGCTCTCTGCTGGAGGACT GGAGAGGCTTGCTGCAGGATGCACTGATGCAGTGCCAGGAGTTCCATGAAATGAGCCATGCCTTGCTTCT CATGCTAGAAAACATTGACAGAAGGAAAAATGAAATTGTCCCAATTGATTCAACCCTTGACCCAGAGACA CTTCAGGACCATCACAAGCAACTCATGCAAATAAAGCAGGAGTTGCTGAAGTCCCAACTCAGAGTGGCCT CACTACAAGACATGTCTCGCCAACTCTTGGTGAATGCTGAAGGTTCAGATTGTCTAGAAGCCAAAGAAAA GGTCCATGTGATTGGAAACCGGCTCAAACTTCTCCTGAAGGAGGTCAGCCATCATATCAAGGATCTTGAG AAGTTACTAGACATGTCAAGCAGTCAGCAGGATTTGTCATCATGGTCTTCTGCAGATGAGCTGGACACCT CAGGATCTGTGAGTCCCACATCCGGAAGAAGTACCCCAAACAGACAGAAATCGCCACGAGGCAAATGTAG TCTCTCACAGCCTGGACCCTCTGTCAGCAGCCCAAAGAGCAGGTCCACAAGAGATGGCTCCGATTCCTCC CGTTCTGACCCCAGGCCAGAACGGGTGGGTCGAGCCTTCCTGTTCCGGATCCTCCGGGCAGCTCTTCCCT TTCAGCTGCTCCTGCTGCTGCTTATTGGACTCACCTGCCTTGTACCCATGTCAGAGAAAGACTACAGCTG TGCCCTCTCCAACAACTTTGCCCGATCCTTCCATCCGATGCTCAGATATACCAACGGTCCTCCTCCACTC TGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-RsrII |
ACCN | NM_001079686 |
Insert Size | 9033 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001079686.1, NP_001073154.1 |
RefSeq Size | 10876 bp |
RefSeq ORF | 9033 bp |
Locus ID | 64009 |
UniProt ID | Q6ZWR6 |
Gene Summary | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and lacks a large portion of the 5' coding region found in variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (3) is shorter and has distinct N-terminus compared to isoform 4. |
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