Ash1l (NM_138679) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8030453L17Rik; Ash1; E430018P19Rik; Kmt2h
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225437 representing NM_138679 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCTAGGAATACTGCTATGTTAGGATTGGGTTCTGATTCCGAAGGATTTTCAAGAAAGAGTCCTT CTACCATCAATCCTGGCACTCTGGCCAGCAAAAGAGAAGCAGAGATAGAGGGTGCCACAGAAGAAGAGGA TCCTCGCAAGCGGAATCGAGAAAGGGGCACAGAAGCAGGAAAGGAGGATGGCTCGACGGATGCACAGCAA CAGTTTTCAGTGAAAGAAACAAACTTTTCAGAGGGAAACTTAAAATTGAAAATTGGCCTTCAGGCAAAGA GAACTAAAAAGCCTCCAAAGAACTTGGAGAACTATGTGTGTCGACCTGCCATAAAGACAACCATCAAGCA CTCAAGGAAAGCACTTAAAAGTGGGAAGATGACAGATGAGAAGAATGAGCACTGCCCTTCAAAATGGGAC TCTTCAAAATTGTTCAAGAAAGCAGGTGACGCTACAGCCATCGACTGCCAAGCCGAAGAAAGCATCCATC TTCATTCACAGGGGGAAAGTAATCCTTTGTCTAAGAAGCTTTCTCCAGTACATTCACAAATGGCAGATTA CATTAGTGCAGCACCATCTCTTGTTGGCAGTCGAGATCCTGATATAAAGGACAGAGCATTACTTAATGGA GGAACTAGTGTAACAGAAAAGTTGGCACAGCTCATTGCTACCTGTCCTCCTTCCAAGTCTTCCAAGGCAA AACCGAAGAAGTTAGGAACTGGCACTACAGTTGGATTGGTTAGCAAGGACTTGATCAGGAAACCAGGTGT TGGCTCTATAGCTGGAATAATTCATAAAGACTTAATTAAAAAGCCAGCTCTTAGCACAGCAGTTGGGTTG GTAACTAAAGATCCAGGGAAAAAGCCTATGTTCAATGCAGCAGTAGGCCTGATCAATAAGGACTCTGTGA AAAAATTGGGAACTGGCACTACAGCCGTATTCATTAATAAAGACTTAGGCAAAAAGCCAGGGGCTATCAC TACAGTTGGACTGCTGAGCAAAGAATCAGGAAAGAAGCTAGGCATTGGTATTGTTCCAGGGCTAGTGAAT AAAGAGTCTGGCAAGAAATTAGGACTTGGCACCGTAGTTGGACTGGTTAATAAAGAGTTGGGAAAGAAAC TGAGTTCTACAGTTGGCCTAGTGGCCAAGGATGTTACGAAGAAGATTGTAGCAAGCTCAGCAATGGGATT GGTTAATAAGGACATTGGAAAGAAATTATTAAATTGTCCTATGGCAGGACAACTGGGCAGTAAAGATGCC TTAAACCTTAAATCTGAAGCACTGCTCCCCACTCAGGAACAACTTAAGGCTTCCTGTAGTGCAAACATAA GTAATCATGACAGTCAAGAACTTCCTGAGTCTCTGAAAGACAGTGCTACCGGCAAAGCTTTTGAGAAGAG TGTTATGCGGCATAGTAAAGAAAGCATGTTGGAAAAATTTTCAGTCCGAAAAGAAATTACTAATTTGGAG AAAGAAATGTTTAATGAAGGAACATGCATTCAGCAAGACAATTTCTCATCTAGTGAAAGGGGAGCCTTTG AAACCTCAAAACATGAAAAGCAGCCTCCTGTGTATTGCACTTCTCCTGACTTTCAAATAGGAGGTGCCTC TGATGCCTCTACAGCTAAATCTCCCTTCAGTGCAGTAGGCGAAAGCAACCTTCCTTCCTCGTCACCTACT GTATCTGTTAATCCTGTAACCAGGAGTCCCCCTGAAGCTTCTTCACAGTTGGTTCCAAACCCATTACTTT TAAATTCTACAGCAGAACAAATGGAAGAAATCTCTGAATCTATTGGAAAGAGCCAGTTTACTGCTGAAAG TACCCACTTGAATGTTGGTCACAGATCATTAGGTCATAGCTTAAGTATTGAGTGTAAAGGAATTGATAAA GAACTAAATGAATCAAAAAATACACATCTAGATATTCCTAGAATAAGTTCTTCCTTGGGAAAAAAGCCAA GTTTGACTTCTGACTCTGGTATTCATGCAATTACTCCTTCCGTTGTTAATTTTACTAGTTTGTTTAGTAA CAAACCTTTTTTAAAGCTTGGTGCAGTCACTGCACCAGACAAACACTGCCAGGTTGCTGAAAGCCTAAGC TCGAGTTTCCAGTCCAAACCATTGAAAAAAAGAAAAGGAAGAAAACCTCGGTGGACTAAAGTGGTGGCAA GAAGCACATGCCGGTCTCCAAAAGGACTAGACTTAGAAAGATCAGAGCTTTTTAAGAATGTTTCTTGTAG CTCACTATCAAATAGTTCTGAGCCAGCGAAGTTTATGAAAACTATCGGAGCATCATCATTCGTCGATCAT GACTTCCTTAAACGCCGATTGCCAAAGTTGAGTAAGTCTTCAGCTCCATCTCTTGCTCTTTTAACCGACA GCGAGAAACCATCTCATAAGTCTTTTATTACTCACAAACTGTCCTCCAGTATGTGTGTCACTAGCGATCT TTTGTCGGATATTTATAAGCCCAAGAGAGGAAGACCTAAATCCAAGGAAATGCCTCAGCTAGAAGGTCCA CCTAAAAGGACTTTAAAAATACCTGCCTCTAAAGTTTTTTCTTTACAGTCTAAAGAAGAGCAAGAACCCC CAATTTTACAACCAGAAATTGAAATTCCTTCCTTCAAGCAAAGTTTGTCTGTATCTCCTTTTCCAAAAAA AAGAGGCAGGCCTAAGAGGCAGATGAGGTCTCCAGTGAAGATGAAACCCCCTGTCCTGTCAGTGGCTCCA TTTGTTGCCACTGAAAGTCCAAGTAAGCTCGAGTCTGAAAGTGAGAACCATAGAAGTAGCAGTGACTTTT TTGAGAGTGAGGACCAGCTTCAGGACACAGACGACTTAGATGACAGTCACAGGCAAAGTGTCTGTAGTAT GAGTGACCTTGAGATGGAACCAGATAAAAAAATTAGCAAGCGAAACAATGGACAATTAATGAAAACAATT ATCCGAAAAATAAATAAAATGAAGACTTTAAAGAGAAAAAAACTGTTGAATCAGATTCTTTCGAGTTCTG TAGAATCAAGTAATAAAGGAAAAGTGCAGTCCAAGCTTCACAATACAGTCTCAAGTCTTGCTGCCACATT TGGCTCTAAATTGGGGCAACAAATCAATGTCAGCAAGAAAGGAACCATTTACATAGGGAAGAGAAGGGGG CGGAAACCAAAAACCGTCTTAAATGGCCTGCTTTCTGGTAGCCCTGCCAGCCTTGCTGTGCTTGAACAAA CAGCTCAGCAGGCAGCTGGGTCAGCATTAGGACAGATCCTCCCCCCTTTACTGCCATCACCTGCTAGTAG CTCTGAGATCCTTCCATCACCTATTTGCTCTCAGTCTTCTGGGACTAGTGGAGGTCAGAGCCCTGTTAGT AGTGACGCAGGCTTTGTTGAACCTAGCTCAGTGCCATATTTGCATGTGCACTCCAGACAGGGCAGTATGA TTCAGACTCTTGCAATGAAGAAGGCTTCCAAGGGCAGAAGGCGGCTCTCTCCTCCTACTTTGTTGCCAAA TTCTCCTTCTCATTTGAGTGAACTCACATCCCTGAAAGAAGCCACTCCTTCCCCAGTTAGTGAGTCTCAT AGTGACGAGACCATTCCCAGTGACAGTGGCATTGGGACAGATAATAATAGCACATCAGACAGGGCAGAGA AGTTTTGTGGACAGAAAAAGAGGAGACATTCTTTTGAGCACATTTCTCTGATTCCCCCTGAAACTTCTAC CGTCCTAAACAGTCTCAAAGAAAAACACAAACACAAATGTAAGCGCAGGAGTCACGATTACCTCAGCTAT GACAAGATGAAAAGACAGAAGCGAAAACGGAAAAAGAAATATCCCCAGCTTCGAAATAGGCAGGATCCAG ACTTCATCGCAGAACTAGAGGAGCTGATAAGTCGTCTAAGTGAGATTCGGATCACCCATCGAAGCCATCA TTTTATTCCCCGAGACCTCCTGCCAACTATTTTTCGAATCAACTTTAATAGTTTCTATACACATCCTTCT TTTCCCTTAGACCCATTGCACTACATTCGGAAACCTGACTTAAAAAAGAAACGAGGGAGGCCCCCTAAGA TGAGGGAGGCAATGGCAGAAATGCCTTTTATGCACAGCCTTAGTTTTCCTTTATCTAGTACTGGATTTTA TCCTTCTTATGGCATGCCTTACTCTCCCTCACCCCTTACAGCTGCTCCAATAGGATTAGGGTATTATGGA AGGTATCCCCCCACTCTTTATCCACCGCCACCATCTCCTTCATTCACCACTCCACTGCCACCTCCCTCCT ATATGCATGCTGGTCATTTATTGCTCAATCCTACCAAATATCATAAGAAAAAGCATAAGCTGCTTAGACA GGAGGCTTTTCTTACAACCAGCAGGACCCCTCTCCTTTCCATGAGCACCTACCCCAGCGTACCTCCAGAG ATGGCTTATGGTTGGATGGTGGAGCACAAACACAGACACCGTCACAAACACAGAGAGCACCGTTCTGAAC AGCCCCAGGTTTCCATGGATAGTGGCTCTTCCAGATCCGTCTTGGAGTCTTTGAAACGTTACCGATTTGG TAAGGATACTGTTGGAGATCGATATAAGCATAAGGAAAAGCACAGGTGTCACATGTCCTGCCCTCATCTC TCTCCTTCAAAGAACTTAATAAACAGAGAAGAACAGTGGGTTTCTCGAGAGCCTTCAGAATCAAGTTCTT TAGCCTTGGGATTGCAAACACCTTTACAAATTGACTGCTCAGAAAGTTCTCCCAGTTTGTCCCTTGGAGG ATTTACTCCCAATTCTGAGCCAGCCAGCAGTGATGAACATATGAACCTTTTTACAAGTGCAATAGGCAGC TGCAGAGTTTCAAACCCTAACTCCAGTTGCCGGAAGAAATTAACTGACAGCCCTGGGCTCTTTCCTGTAC AAGATACTGCACTAAATCGGCCTCACAGAAAGGAACCATTACCTTCCAGTGAAAGGGCAATACAGTCGTT GGCAGGTTCCCAGTCAGCTTCCGATAAACCTTCCCAGCGATCATCAGAAAGCACAAATTGTAGCCCTACT CGTAAAAGGTCTTCATCTGAGAGTACTTCTTCAACAGTGAATGGAGTTCCTTCCAGAAGTCCCAGATTGG TTGCTTCTATGGATGATTCTGTAGACAGTCTGTTGCAGCGGATAGTACATCACGATGAGCAAGAGTCCAT GGAGAAAAACGGTGATGCTTCCATTACAACTGTGTCTGCACCACCTTCTTCCAGTCCAGGCCATAGCTAC AGCAAGGAGCGAGCCTTAGGTAAATCAGACAGCCTTCTAGTGCCTGCAGTCCCGAATGACTCTTGCAGTA ATATCCCACTTCTTTCTGAAAAGTCAGCAAGTAGGTGTTCCCCCCATCACATCAAGAGAAGTGTAGTTGA AGCTATGCAACGCCAAGCTCGGAAAATGTGCAATTATGACAAAATCTTGGCCACCAAGAAAAACCTGGAC CATGTTAATAAAATTTTAAAAGCCAAAAAACTTCAAAGGCAGGCCAGGACAGGAAACAATTTTGTGAAAC GCAGACCAGGTCGACCTCGGAAATGCCCCCTCCAAGCAGTGGTGTCAATGCAAGCCTTCCAGGCCGCCCA GTTTGTCAGCCCGGAATTGAATGAAGGCGAAGACATGTCCCTGCACCTAAGTCCAGACACAGTCACTGAT GTGATCGAGGCTGTGGTTCAGAGTGTGAACCTCACTTCAGAACATAAGAAGGGGGTGAAGAGGAAAAATT GGCTGTTAGAAGAACAGACTAGGAAAAAGCAGAAGACAGTACCAGAGGAAGAAGAGCAAGAAAACAATAA AAGCTTTATAGAAACACCAGTTGAAATTCCCAGTCCTCTTGAAACGCCTGCTGAACCTTCTGAACCTGAA AATACCTTGCAACCTGTGCTGGCTCTCATCCCACGAGAAAAGAAGGCCCCACGTCCTCCAAAGAAGAAAT ACCAGAGAGCTGGGCTGTATTCTGATGTTTACAAAACAATAGACCCAAAGAGCCGATTAATCCAATTAAA GAAAGAAAAGTTGGAGTATACTCCAGGAGAGCACGAGTATGGGTTGTTCCCAGCGCCCATTCATGTTGGA AAGTATCTCAGACAGAAGAGAATTGACTTCCAGCTTCCCTATGATATTCTCTGGCAGTGGAAACACAATC AGCTATACAAAAAGCCAGATGTCCCCCTGTATAAAAAAATCCGTTCAAATGTCTATGTTGATGTCAAACC ACTTTCTGGTTATGAGGCTACCACCTGTAATTGTAAGAAGCCAGATGACGACACCAGGAAGGGCTGTGGG GATGACTGCCTAAATAGAATGATCTTTGCTGAGTGTTCCCCTAACACTTGCCCCTGTGGTGAGCAGTGCT GTAACCAGAGGATACAGAGGCATGAGTGGGTGCAGTGTCTGGAACGATTTCGAGCTGAGGAAAAAGGTTG GGGAATTAGAACCAAAGAACCCCTAAAAGCTGGCCAGTTCATCATTGAATACCTAGGGGAAGTTGTCAGC GAACAAGAATTCAGGAACAGGATGATTGAGCAATATCATAATCACAGTGACCACTACTGTTTAAACCTGG ATAGCGGAATGGTGATTGACAGTTACCGCATGGGAAATGAGGCCAGATTCATCAACCACAGCTGTGACCC AAATTGTGAAATGCAGAAATGGTCTGTTAATGGAGTGTACCGTATTGGACTGTATGCACTTAAGGATATG CCAGCTGGGACAGAGCTTACCTATGATTACAACTTTCATTCCTTCAATGTAGAAAAACAGCAACTTTGTA AATGTGGTTTTGAGAAGTGTCGAGGAATCATTGGAGGCAAGAGTCAACGGATGAACGGACTCCCTAGCCA TAAAGGTAGCCAGTCTTCAAGCACGCATAGAAAGTCTGCACGGGCGAAAGAGAAGCGGAAGTCCAAGCAC AAGCTGAAGAAAAGGAAAGGCCATCCCTCTGAAGAGCCCAGTGAAAACATCAACACCCCAACAAGATTGA CTCCACAGTTACAGATGAAGCCAATGTCCAACAGAGAGAGGAACTTCGTGCTAAAGCACCATGTGTTCTT GGTGCGAAACTGGGAAAAGATCCATCAGAAGCAGGAGGAGGTGAAGCACACCCGTGACATCCACTCAGCA TCGCTGTACACCCGCTGGAACGGCCTCTGTCGGGACGACGGGAACATTAAGTCTGATGTCTTCATGACCC AGTTCTCTGCCCTGCAGACAGCTAGATCTGTTCGAACAAGACGCTTGGCAGCTGCAGAGGAAAACCTGGA AGTGGCTCGGGCAGCGCGCCTAGCACAGATCTTCAAAGAGATCTGTGATGGCATCATCTCCTACAGAGAT TCTTCCCAGCAAACCCTTGCAGCTCCACTTTTGAATCTTCCCCCAAAGAAAAAAAATGCTGACTATTATG AGAAGATCTCTGACCCCCTGGATCTCAGCACCATAGAGAAGCAGATCCTCATTGGGTATTACAAAACAGT GGAGGCTTTCGATGCTGACATGCTTAAGGTCTTTCGGAATGCTGAGAAGTACTATGGGCGTAAATCCCCA ATTGGGAGAGATGTTTGCCGCTTACGAAAGGCCTATTACAGCGCCCGGCATGAAGCTTCGGCTCAGATCG ATGAGATTGTGGGAGAAACGGCCAGTGAGGCCGACAGCAGCGAGACCTCAGTCTCCGAGAAGGAGAGCGG GCACGAGAAGGATGATGATGTTATCCGCTGCATCTGCGGCCTCTATAAAGATGAAGGCCTCATGATCCAG TGTGACAAGTGCATGGTGTGGCAGCACTGCGACTGCATGGGTGTGAACACGGATGTGGAGCACTACCTTT GTGAGCAGTGTGACCCTCGGCCTGTGGACAGGGAGGTACCCATGATCCCTAGGCCCCACTATGCCCAGCC CGGCTGTGTCTACTTCATCTGTTTACTCCGAGATGACTTGCTGCTTCGTCAGGGTGATTGTGTGTACCTG ATGAGGGACAGTCGGCGCACGCCTGATGGCCACCCAGTCCGTCAGTCCTACAGACTCTTATCTCACATTA ACCGAGATAAACTTGACATCTTTAGAATCGAGAAGCTCTGGAAGAATGAAAAAGAAGAACGGTTTGCCTT TGGTCACCATTATTTCCGCCCACATGAGACTCATCACTCTCCATCCCGTCGGTTCTATCACAACGAGCTG TTCCGGGTGCCCCTCTATGAGATCATTCCCTTGGAGGCCGTAGTTGGGACCTGCTGTGTGTTGGACCTTT ATACATACTGTAAAGGGAGACCCAAAGGAATAAAGGAACAAGATGTGTATATCTGTGATTATCGGCTTGA CAAGTCAGCACACCTTTTTTACAAGATTCATCGGAATCGATATCCGGTCTGCACCAAACCCTATGCCTTT GATCATTTCCCCAAGAAGCTCACTCCCAAAAGAGACTTCTCACCTCATTATGTCCCAGACAACTACAAGA GGAACGGAGGACGATCATCCTGGAAGTCTGAACGCTCAAAGCCACCCCTAAAAGACCTAGGACAAGAAGA TGATGCTTTGCCCTTGATTGAAGAGGTACTGGCTAGTCAGGAGCAAGCAGCCAGGGAAGTGCCCAGCCCT GAGGAGCCAGACCAGGAAAGAGCCACTGGGGACATTGGGGACGCTGAAAAGAAACCAGAGGAGAGCAGTC AAGAAGCCCAGTTAGCCAGCACACCTGAGGAGCGAAGGCACAGCCAGAGGGAACGGCTCAACCAGATCCT GCTCAACCTCCTTGAGAAAATACCTGGGAAAAATGCCATTGACGTGACCTACCTGCTGGAGGAAGGGTCC GGCAGGAAACTGCGAAGGCGCACTTTGTTTATACCAGAAAATAGCTTTCGGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_138679
Insert Size	8877 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138679.5, NP_619620.3
RefSeq Size	11293 bp
RefSeq ORF	8877 bp
Locus ID	192195
UniProt ID	Q99MY8
Gene Summary	Histone methyltransferase specifically methylating 'Lys-36' of histone H3 (H3K36me) (By similarity). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). The physiological significance of the H3K9me1 activity is unclear (Probable) (PubMed:22939622).[UniProtKB/Swiss-Prot Function]

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