Fbn2 (NM_010181) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC063774; Fib-2; mKIAA4226; sne; sy
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225428 representing NM_010181 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGACGGCGGCGGTTGTGTCTCCAGCCCTACTTCGTGTGGCTGGGTTGCGTGGCGCTCTGGGCGC AGGGCACAGATGGCCAGCCCCAGCCTCCTCCACCAAAGACGCTCCGGCCCCAGCCGCCCCCGCAACAGGT CCGACCCGCAGTAGCGGGCTCCGAAGGTGGCTTCATGGGGCCTGAGTATCGGGACGAGGGCGCGGTAGCA GCGAGCCGGGTCCGCAGGCGAGGACAGCAGGAAATACTCCGAGGGCCCAACGTGTGCGGTTCTAGATTCC ACTCCTATTGCTGCCCAGGATGGAAGACGCTCCCTGGAGGAAATCAATGCATTGTCCCCATTTGCAGGAA CAGTTGTGGAGATGGATTCTGTTCCCGCCCCAACATGTGTACTTGTTCCAGTGGACAAATATCCCCGACA TGCGGAGCCAAATCAATTCAGCAGTGTAGCGTGAGGTGCATGAACGGCGGGACCTGTGCAGACGACCACT GTCAGTGCCAGAAGGGATACATCGGAACTTACTGTGGACAACCTGTCTGTGAGAATGGATGCCAGAACGG GGGGCGTTGTATCGGCCCTAACCGCTGTGCTTGCGTTTATGGATTCACCGGGCCGCAGTGCGAAAGAGAT TACAGGACAGGCCCGTGTTTCACTCAAGTCAATAATCAGATGTGCCAGGGGCAGCTGACAGGCATCGTCT GCACAAAGACACTGTGTTGTGCCACCATCGGACGAGCCTGGGGCCATCCTTGTGAGATGTGTCCAGCCCA GCCTCAGCCCTGCCGCCGGGGCTTCATTCCTAACATCCGCACTGGAGCATGTCAAGATGTTGACGAATGC CAGGCGATTCCAGGACTGTGCCAGGGGGGAAACTGTATCAACACAGTGGGCTCGTTTGAGTGCAGATGCC CTGCTGGTCACAAGCAGAGTGAAACCACACAGAAATGTGAAGACATTGATGAGTGCAGTGTGATTCCTGG GGTCTGTGAGACTGGCGACTGTTCCAACACCGTGGGAAGCTATTTTTGTCTCTGTCCCCGTGGCTTCGTA ACCTCAACAGATGGCTCGCGTTGCATTGATCAGAGGGCTGGAACATGCTTCTCCGGCCTGGTGAATGGCC GTTGTGCCCAAGAACTCCCGGGCCGAATGGCAAAAGCTCAATGTTGTTGTGAGCCCGGCCGTTGCTGGAG CATCGGGACCATTCCGGAGGCCTGTCCTGTCAGAGGTTCCGAGGAATATCGTAGACTGTGCCTGGATGGT CTTCCGATGGGTGGCATTCCAGGGAGCTCGGTTTCTAGGCCTGGGGGCACGGGGAGCACCGGCAATGGCT ATGGCCCCGGTGGAACAGGCTTCCTCCCCATCCCTGGAGACAATGGCTTTTCTCCGGGTGTTGGGGGAGC TGGAGTAGGAGCTGGGGGACAGGGACCCATCATCACGGGACTAACAATTCTCAACCAGACAATAGACATC TGTAAGCACCATGCCAATCTCTGTTTGAATGGACGGTGTATACCTACTGTTTCTAGCTACAGATGCGAGT GTAATATGGGCTACAAGCAGGATGCTAATGGAGATTGTATAGATGTTGATGAATGTACATCCAATCCCTG CAGTAATGGAGATTGTGTTAACACACCTGGATCCTATTACTGTAAATGTCACGCTGGATTCCAGAGGACT CCTACGAAACAAGCGTGCATCGATATCGATGAGTGCATCCAGAATGGAGTTCTGTGTAAGAATGGCCGAT GTGTGAACACAGATGGCAGTTTCCAGTGCATTTGTAATGCTGGCTTTGAATTAACCACAGACGGAAAAAA CTGTGTTGACCATGATGAATGTACAACGACCAACATGTGCTTGAATGGAATGTGTATCAACGAAGATGGC AGCTTCAAGTGCGTGTGCAAGCCAGGCTTCATCCTGGCTCCAAACGGCCGCTACTGCACAGACGTGGACG AATGCCAGACCCCAGGAATCTGCATGAACGGGCACTGCATCAACAACGAAGGATCCTTCCGCTGTGACTG TCCTCCCGGCCTGGCTGTGGGTGTGGACGGACGTGTATGTGTTGACACTCATATGCGTAGCACCTGCTAC GGGGAAATCAAGAAAGGAGTGTGTGTGCGTCCATTCCCGGGTGCGGTGACCAAGTCTGAATGCTGCTGTG CTAATCCAGACTATGGTTTTGGAGAGCCTTGCCAGCCTTGCCCCGCTAAAAATTCAGCTGAGTTCCATGG CCTCTGTAGCAGTGGCATCGGCATCACTGTGGATGGAAGAGATATTAACGAGTGTGCCTTGGACCCTGAC ATATGTGCCAATGGGATCTGTGAAAACCTGCGAGGCAGCTACCGCTGTAACTGCAACAGTGGCTATGAAC CAGATGCCTCAGGAAGAAATTGCATTGACATTGATGAATGCCTGGTTAATAGGCTGCTTTGTGACAACGG CCTATGCAGAAATACCCCTGGCAGTTACAGCTGCACGTGTCCCCCTGGCTATGTGTTCCGGACGGAGACA GAGACCTGCGAAGATGTAAATGAATGTGAGAGCAACCCGTGTGTCAATGGAGCCTGCAGGAACAACCTGG GCTCTTTCCATTGCGAGTGCTCGCCCGGCAGCAAGCTCAGCTCCACCGGACTCATCTGCATCGACAGCCT GAAAGGAACCTGCTGGCTCAACATCCAGGACAACCGCTGTGAGGTGAACATTAATGGAGCCACCCTGAAA TCCGAATGCTGTGCCACACTTGGAGCCGCTTGGGGGAGCCCCTGTGAGCGCTGTGAGCTAGATGCAGCTT GCCCAAGAGGGTTTGCCAGGATCAAAGGTGTGACTTGCGAAGACGTTAATGAATGCGAGGTGTTTCCTGG CGTCTGTCCAAATGGACGCTGTGTCAACAGCAAAGGCTCTTTCCACTGTGAATGCCCTGAAGGTCTTACT CTGGATGGTACCGGCCGGGTGTGTTTGGATATCCGTATGGAGCACTGCTTCCTGAAGTGGGATGAGGATG AGTGCATCCACCCTGTCCCTGGGAAGTTCCGCATGGACGCCTGCTGCTGTGCAGTCGGGGCAGCTTGGGG CACAGAGTGTGAAGAATGTCCTAAGCCTGGCACCAAGGAATATGAGACCCTGTGCCCGCGGGGCCCAGGC TTTGCTAACAGAGGGGACATCCTCACTGGGCGTCCATTTTACAAAGATATCAACGAGTGCAAAGCCTTTC CTGGGATGTGCACCTATGGGAAGTGCAGAAACACAATTGGAAGCTTCAAGTGCCGTTGCAATAATGGCTT CGCCCTGGACATGGAGGAAAGAAACTGTACTGACATTGATGAGTGCAGGATCTCCCCCGACCTCTGCGGC AGCGGCATCTGTGTCAACACGCCAGGCAGCTTTGAATGCGAGTGCTTTGAGGGCTACGAGAGCGGCTTCA TGATGATGAAGAATTGCATGGACATTGACGAATGTGAACGTAACCCTCTCCTTTGTAGGGGTGGCACCTG CGTGAACACTGAGGGAAGCTTTCAGTGTGACTGCCCACTGGGACACGAGCTGTCACCGTCCCGTGAGGAC TGTGTAGATATTAACGAGTGCTCCTTGAGCGACAACCTCTGTAGAAATGGCAAATGTGTGAACATGATCG GAACTTACCAGTGCTCTTGCAATCCTGGATACCAGGCCACGCCAGACCGCCAGGGCTGTACAGATATCGA CGAGTGTATGATAATGAATGGGGGCTGCGACACCCAGTGCACCAACTCCGAAGGAAGCTACGAGTGCAGC TGCAGCGAGGGGTACGCACTGATGCCAGACGGACGGTCGTGCGCAGATATTGATGAGTGTGAAAATAACC CCGATATCTGTGACGGCGGCCAGTGTACCAACATTCCTGGGGAGTATCGCTGTCTCTGTTATGATGGCTT CATGGCTTCGATGGACATGAAGACATGCATCGATGTGAACGAGTGTGACCTGAATCCCAACATCTGCATG TTTGGGGAATGTGAGAACACGAAGGGCTCTTTCATTTGCCACTGCCAGCTGGGCTACTCGGTGAAGAAGG GGACCACAGGATGCACAGACGTGGACGAGTGTGAGATTGGCGCCCATAACTGCGACATGCACGCCTCATG TCTGAATGTCCCTGGAAGCTTCAAGTGCAGCTGCAGAGAAGGCTGGGTGGGAAATGGCATCAAATGCATT GATCTGGATGAGTGCGCGAATGGAACCCACCAGTGCAGCATAAACGCCCAGTGTGTCAACACCCCAGGCT CCTACCGGTGCGCCTGCTCGGAGGGCTTCACGGGAGATGGCTTTACTTGCTCAGATGTTGACGAGTGTGC GGAAAACACAAACCTTTGTGAGAACGGCCAGTGCCTGAATGTCCCGGGAGCTTACCGCTGCGAGTGTGAG ATGGGCTTCACCCCAGCCTCAGACAGCAGGTCCTGCCAAGATATTGATGAATGCTCCTTCCAAAATATCT GTGTCTTTGGAACCTGTAACAACTTGCCCGGGATGTTTCACTGTATCTGTGATGATGGTTATGAACTGGA CAGGACAGGAGGAAACTGTACAGATATTGATGAGTGCGCAGACCCCATAAACTGTGTCAATGGCCTATGT GTGAACACGCCTGGTCGCTATGAGTGTAACTGCCCGCCTGACTTCCAGCTGAACCCCACTGGCGTGGGTT GCGTTGACAACCGCGTGGGCAACTGTTACCTGAAGTTTGGTCCTCGAGGAGACGGGAGTCTGTCCTGCAA CACAGAGGTCGGCGTAGGTGTCAGTCGCTCCTCCTGCTGCTGCTCCCTGGGGAAGGCCTGGGGAAACCCC TGTGAGACATGCCCTCCTGTAAACAGCACTGAGTATTATACCCTGTGTCCTGGAGGCGAAGGCTTTAGAC CCAACCCCATCACAATCATCTTAGAAGACATTGATGAATGTCAGGAGTTACCAGGGCTCTGCCAGGGTGG AAACTGTATCAACACTTTTGGGAGTTTCCAGTGTGAGTGCCCACAGGGTTACTACCTCAGTGAGGAAACC CGCATCTGTGAAGATATCGACGAGTGCTTTGCCCATCCCGGCGTCTGTGGACCTGGCACCTGCTACAACA CCCTGGGAAATTACACTTGCATTTGCCCGCCTGAGTACATGCAAGTCAATGGAGGACATAACTGTATGGA CATGAGGAAAAGCTTCTGCTACCGAAGCTATAATGGGACCACATGTGAAAACGAGCTGCCTTTTAACGTG ACAAAGAGGATGTGCTGCTGCACATACAATGTCGGCAAAGCCTGGAACAAACCTTGTGAGCCGTGCCCAA CCCCAGGGACAGCTGACTTTAAGACCATATGTGGAAATATTCCTGGATTCACCTTTGACATTCACACAGG AAAAGCTGTTGACATTGACGAGTGTAAAGAGATTCCGGGCATCTGTGCAAACGGTGTGTGCATTAACCAG ATCGGTAGCTTCCGCTGCGAATGCCCCACAGGCTTCAGCTACAACGACCTGCTCTTGGTCTGTGAAGACA TCGATGAGTGTAGCAACGGCGACAATCTATGCCAACGAAACGCGGACTGTATCAACAGCCCCGGCAGCTA CCGCTGCGAGTGTGCGGCCGGCTTCAAACTCTCCCCCAACGGGGCCTGTGTAGATCGCAATGAATGCTTG GAAATTCCTAATGTTTGCAGTCATGGGTTGTGTGTTGATCTGCAAGGGAGTTACCAGTGTATCTGCAACA ATGGCTTTAAGGCTTCTCAAGACCAGACCATGTGCATGGATGTCGACGAGTGTGAGCGGCACCCGTGCGG AAACGGGACTTGTAAGAACACTGTGGGATCCTACAACTGTCTGTGTTACCCTGGGTTCGAGCTCACTCAC AACAACGATTGCCTAGACATAGACGAGTGCAGCTCGTTTTTTGGCCAGGTGTGCAGAAATGGGCGGTGCT TCAATGAAATCGGCTCCTTCAAGTGCCTGTGCAACGAGGGTTACGAACTGACTCCAGACGGCAAAAACTG TATCGATACTAATGAGTGTGTCGCACTTCCTGGCTCCTGTTCTCCTGGGACCTGTCAGAATTTGGAGGGC TCCTTCAGGTGCATCTGCCCCCCTGGATATGAAGTGAGAAGCGAAAATTGCATTGATATAAATGAATGCG ATGAGGACCCCAACATTTGCCTTTTTGGTTCCTGCACCAACACCCCAGGGGGCTTCCAGTGCATCTGTCC CCCTGGCTTTGTCTTGTCTGACAACGGGCGGAGATGCTTCGATACTCGCCAGAGCTTCTGCTTCACCAAC TTTGAAAACGGAAAGTGTTCTGTACCCAAAGCCTTCAACACCACCAAGGCAAAATGCTGTTGCAGCAAGA TGCCAGGCGAGGGCTGGGGGGACCCGTGTGAGCTGTGCCCCAAAGATGATGAAGTTGCCTTCCAGGATCT ATGTCCATACGGCCACGGGACAGTCCCTAGTCTTCATGACACACGTGAAGATGTCAATGAGTGTCTTGAG AGCCCAGGCATTTGCTCAAATGGTCAGTGCATCAACACTGATGGATCTTTCCGATGTGAGTGTCCAATGG GCTACAACCTGGATTACACTGGAGTCCGGTGTGTGGACACCGACGAGTGCTCCATCGGCAACCCCTGCGG GAACGGGACGTGCACCAACGTGATCGGGAGCTTCGAATGCACCTGCAATGAAGGCTTTGAGCCGGGGCCC ATGATGAACTGCGAAGACATCAACGAGTGTGCCCAGAACCCGCTGCTCTGTGCTTTCCGATGCATGAACA CTTTCGGATCTTATGAATGCACGTGCCCAGTTGGCTACGCCCTCAGGGAAGACCAAAAGATGTGTAAAGA TCTGGACGAGTGTGCCGAGGGGCTGCACGACTGCGAGTCTAGAGGCATGATGTGCAAGAACCTGATCGGC ACCTTCATGTGCATCTGCCCCCCAGGCATGGCCCGCAGGCCCGATGGCGAGGGCTGTGTAGATGAGAACG AATGCAGGACTAAGCCAGGCATCTGTGAAAATGGACGCTGCGTTAACATCATTGGGAGCTACAGGTGTGA GTGCAATGAGGGATTCCAGTCGAGTTCCTCCGGCACTGAATGCCTCGACAACCGCCAGGGTCTGTGCTTC GCCGAGGTACTGCAGACAATGTGTCAGATGGCCTCCAGTAGCCGCAACCTCGTCACAAAGTCGGAGTGCT GCTGTGACGGTGGCCGAGGCTGGGGCCACCAGTGTGAGCTGTGCCCACTTCCAGGAACCGCCCAGTACAA AAAGATATGTCCTCACGGCCCAGGATACGCCACTGATGGAAGAGATATCGATGAATGTAAGGTGATGCCA AGCCTCTGCACTAATGGCCAGTGCGTCAACACCATGGGCTCCTTCCGGTGCTTCTGCAAAGTTGGCTACA CCACGGACATCAGCGGAACAGCTTGTGTAGACCTCGATGAATGTTCGCAGTCTCCAAAGCCATGCAACTT CATCTGCAAGAACACCAAGGGCAGTTACCAGTGCTCCTGCCCACGGGGGTACGTCCTGCAGGAGGACGGA AAGACGTGCAAAGACCTCGACGAATGTCAAACCAAACAGCACAACTGCCAGTTCCTCTGTGTCAACACCC TGGGGGGATTCACCTGTAAATGTCCGCCCGGTTTCACCCAGCATCACACGGCTTGCATCGATAACAATGA ATGTGGGTCTCAGCCTTCCCTCTGTGGTGCAAAGGGGATCTGTCAGAACACTCCTGGGAGCTTCAGCTGC GAATGCCAAAGAGGGTTCTCTCTGGATGCCTCTGGGCTGAACTGTGAAGATGTCGATGAGTGTGATGGGA ATCACAGGTGCCAACACGGCTGCCAGAATATCCTGGGAGGCTATCGGTGCGGCTGCCCGCAGGGCTACGT CCAGCACTACCAGTGGAACCAGTGTGTTGATGAGAATGAATGTTCCAACCCAGGTGCCTGTGGCTCCGCC TCCTGCTACAACACCCTGGGGAGTTACAAGTGCGCCTGCCCCTCCGGGTTCTCCTTCGACCAGTTCTCCA GCGCCTGCCATGATGTGAATGAGTGTTCCTCCTCCAAGAACCCATGCAGCTACGGCTGCTCCAACACAGA AGGCGGCTACCTCTGCGGCTGCCCACCAGGGTACTTCAGAGTGGGACAGGGCCACTGTGTCTCGGGAATG GGCTTCAACAAAGGACAGTACCTGTCGGTGGATGCGGAGGCAGAGGATGACGAAAACGCTCTGTCCCCAG AAGCATGCTACGAGTGCAAAATCAATGGCTACACCAAGAAAGATGGCCGACGGAAGAGGAGCGCGCAGGA GCCTGAGCCCGCCTCAGCCGAGGAGCAAATCAGCCTGGAGAGCGTAGCCATGGACAGCCCAGTCAACATG AAGTTCAACCTCTCCGGCCTTGGCTCCAAGGAGCACATCCTAGAACTGGTCCCCGCCATTGAGCCCCTCA ACAACCACATCCGTTATGTCATCTCGCAAGGGAACGAGGATGGCGTCTTCCGAATCCACCAAAGGAACGG GCTCAGCTACCTGCACACGGCCAAGAAGAAGCTCGCTCCCGGCACATACACACTGGAAATCACCAGCATC CCCCTCTATGGGAAGAAGGAGCTGAGGAAATTGGAGGAGCACAATGAGGACGACTACCTTCTGGGGGTGC TCGGGGAGGCTCTCCGAATGAGGTTGCAGATTCAGTTGTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010181
Insert Size	8724 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010181.2, NP_034311.2
RefSeq Size	10480 bp
RefSeq ORF	8724 bp
Locus ID	14119
UniProt ID	Q61555
Gene Summary	Fibrillin-2: Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively (PubMed:20855508).[UniProtKB/Swiss-Prot Function]

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