Zzef1 (NM_001045536) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8430405D05Rik; BC051585; C85424; C130099L13Rik; mKIAA0399; NSPA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225427 representing NM_001045536 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACGCTCCGAGTAACAGCAGCGAAGACGAAGCGGCGGCCGCAGGGGGTGAGGGCTGGAGCCCAC ATCAAGATTGGGCTGCCGACTCTGGCACGACCCCCGGCCCCGGCCCTGCGGCCGCGGTGCTGCCTTCCGC CGCTGCACTCCTGGAACCCGCTCGGCTGCGAGAGGCAGCGGCTGCGTTGCGGCCCGCACCTCCCTGCGAA TCCCTGGTGTCGAGGCACCACGGCGCGCTCTTGCGCTGGCTGGAAGAGCGGCTGGGCCGCGGTGAAGAGT CCGTCACCCTGGAGCAGTTCCGGGAGTTGCTGGAGGCTCGCGGCGCTGGATGTTCGGGCGAGCAGTTCGA GGAGGCCTTTGCCCAGTTTGATGCTGAGGGAGATGGGACAGTTGATGCTGAGAATATGCTGGAGGCCCTC AAGAACTCCAGTGGAGCCAATCTTCAGGGGGAGCTGAGCCATGTCATCAGACAACTGCAAGCCTGCTCTC TTGTTCCAGGTTTCATCGACATATTTTCAGAGTCAAAGGAGGGCCTTGGCATTCACTCATCAATGATATT GCGCTTCCTGCATCGCAATCGGATCTCTAGCATGGTGATTCCCTACCCAATGTTAGACCACTGTAATAAC ATGTGCACCATGAGATCATCAGTTCTGAAGGAGTCTCTGGATCAACTGGTACAAAAAGAAAAGGAAAGCC CTGGAGATCTAGCCAGAAGCCCAGAGATGGATAAGCTCAAGTCAGTAACCAAGTGTTATGCTTATATAGA AACGTCCTCCAACCCTGCAGACATATACAGGATGACAAATGGAGAAACATCATCCTACTGGCAGTCAGAT GGCAGTGCGCGCTCACACTGGATACGTTTGAAGATGAAGCCAGATGTTGTGCTTAGGCATCTGTCCATTG CAGTGGCTGCCACTGACCAGAGCTACATGCCACAGCAGGTGACAGTGGCTGTGGGAAGGAGTGCCAGTGA CCTGCAGGAAGTTCGTGATGTGCACATCCCCAGCAATGTCACTGGCTATGTGACACTGCTAGAAAATGCC AACATCAGTCAGCTCTATGTCCAGATAAACATAAAGCGTTGTCTTAGCGACGGATGTGACACTAGGATTC ACGGTCTGAGGGCTGTTGGCTTTCAGAGAGTAAAGAAATCTGGAGTCTCTGTCTCTGATGCCTCTGCAAT ATGGTATTGGTCTCTGCTGACATCTTTGGTGACAGCGTCTATGGAGACAAATCCTGCCTTTGTCCAGACA GTGCTGCACAATACTCAGAAGGCACTGCAGCACATGCCACCGCTGTCCCTCTCTCCAGGATCTACGGACT TCTCAACTTTCCTCTCTCCTAATGTTCTGGAAGAAGTGGACAGCTTCCTCATAAGGATAACTAGCTGCTG TTCTACTCCAGAGGTAGAGCTGACTCTTCTGGCTTTTGCACTAGCAAGAGGAAGTATTGCCAAAGTAATG AGCTCTCTGTGTACCATCACTGACCACTTGGACACACAGTATGACGCCTCATCCCTCATCTCATCCATGG CATCTGTCAGGCAGAACCTGCTCCTGAAATATGGTAAGCCTCTCCAGTTGACTCTTCAGGCTTGTGATGT TAAAGGAAAAGAAGATAAGTCAGGACCTGAAAATCTTCTTGTGGAGCCATGGACAAGGGATGGTTTTCTT ACAGAGACTGGAAAAACGAGAGCAAGTACTATTTTTTCTACAGGAAGTGACTCTGCCTTCCAAGTTACAC AGATCAGAATTATGGTTCGCCGTGGTGGCATTGGTGCTCAGTGTGGGCTGGTCTTTGCCTATAACTCGCC TTCTAATAAGTTTCACGCAGAAGAACACTTCAAACGGTTTGAAAAATATGACAAATGGAAACTCCAGGAA CTGAGGCAGTTCGTAAAAAGCAGGATTGGTTGCTCATCTGATGACCTTGGAGAGGATGATCCTATTGGCT GGTTTGAGCTAGAAGAAGAGTGGGATGAAGCAGATGTCAAGTTACAACAGTGCAGAGTTGCCAAATTCTT GATGGTGAAGTTCCTCTGCACCCGCCAGGAGTCAGCTGAACGGTTGGGCGTGCAAGGCTTAAGCATCAGT GGGTACCTCCGGCCTGCAAGGGCAGAAGCGGAACAAAGCATCCTCTATGCCCACTGTAGAAGGGACACAG AGAATATTCATGGGGCCACCCTGCTTCTTAGGACTCTGCAGTTCATCCAGCAGCTTTCCCATGACCTGAT GCAGCAAAAGGAAAGTGGCTTAAAACATAAGTCTTTCCTGGACTTTGCGGGTCTTGATTTGCAGATCTTC TGGAAATTTTACAGTAAATTAAAGCAAAACCGAAGGGAAGAATGTATCTGTGCACAGACCCTTCTGCTGA AGCTCCTGCAGAGCTGCTTCTCAGTGCTGCAGGGTGACCCACAAGCTGCCTCAGAGGAGGAGAAGCCTAC AGCGCAACGCTCTGAGGGAATACAGGCTGCCAAGGAGCTCTACACACACTTATGTAATGTGGTAGATAAA CCTAATGGAAATTCCATGCCCATGGAGATCCTAAAACAAGAAGTGAGAAACACTCTTCTCAATGGGGCTG CCATCTTCTTTCCTGATCGACAGACCCGGAGAAGCCAGCTCTTCACCATGATGAAGAGTGTCACTGAGCA CGAACGCAAGCAGTCTCTGCAACTCACTTTCCATTCACTCTGCACATATTTTAGTGATAAGGATCCAGGA GGCCTTCTGCTTTTACCTGAGAAGAGTGACCTGGCCACCATGAACACCAGTGAAGTCCTGGCTGTCATGA ACACCCTCCTCTCTGTTGCTGCTCGAGAGTGTGAATTGTTGATGCTCAATCGGTCCCATGGGGCAGTTGG TTCTGTACTCTTCTCCCTGTTCTGGTCTGTGCAAGGCAGCCTGCTCTCCTGGTGCTTCCTGCAGCTCAAG AGCACAGATGCTGCAGCCAAAGAGCTCGCCATGGATCTTATTGAAAAATATGTGGGGCAGTTTCTGGCAA GCATGAGAGTGATTTTGGAGTCCCTTTTGTCACAGTATAGTGGAAAGACCATAGTAGAAAAATTATGCAA TTCGGTGTTTTCCATGGCAGCTCGTCAACTGGTCATCTTCCTGCTAGACTTCTGCACTTTAGACGTGTCC CACTGCACACTCCTAAGAGAGTTCAGCACCCTCACAGAGCTGTTGAAGAAGCTGTGCAGTGACCCTGAAG GAGGCCTGAGCAAGTTGGATGTGGAGACCTGGCAGCAGGAGCAGCCTGTGGTATTGCATACCTGGACCAA GGAATCCACCCACAACTATGAAAATAACTGCCATGAAGTGTCTGTCTTTATTAGCCCAGGAGCAACTTAT TTTGAGGTGGAATTTGATGAGAGGTGTGAGACTGAGAAAAGGTACGATTATTTGGAATTTACTGACTCTA GAGGTGGGAAGACACGCTATGACACCAAAGTTGGGACTTATAAATGGCCCAAGAAAGTGACCTTTAAGGA TGGCCCTCGGCTGCAGTTCCTCTTTCATTCTGACAGCAGTAACAATGAGTGGGGCTACAAGTTCACTGTC ACTGCCTATGGTCTGCCTGATGTTGCTGTGTCTTGGGGTTTGGACTTGCAACTCCTTGTCTCCCGACTGA TGGGGCGCCTTGCTTCCCAGTGCATGGCACTCAAGTCAGTGCATCAGTTAGGCAGTAACATGGCAGTATC CCAGGCAAAACTGACTTCAGTCCTGAACTCTCCATTGTGGAAACCTGTCTTCAGGCATCAGATTTGTCCA GAGTTGGAATTGGAAGCAAGCTGGCCCACTCACCCACACAAGGATGGGAAGGAGGTCAAGAACATCCCTG ATGATCCCTGCCGCCATTTTCTCCTTGATTTTGCCCAGTCAGAGCCTGCTCAGAACTTCTGTGGGCCATA TTCAGAACTTTTCAAAGGATTCATACAGGCATGTAGAAAACAGGCCCCAAAGACAGATATAGTTGCTGGT TCCACTATTGATCAAGCTGTGAACGCCACCTTTGCCGCTCTGGTGTATCGCACTCCAGACTTATATGAGA AGCTGCAAAAATATGTTAACAGCGGTGGCAAGATAGCCCTGACTGAGGAGTTCTCTCAGGTGTATTCCCT GGCAGATGGGATTCGAATATGGATGTTAGAGATGAAGCAGAAGTCCTTGCTGAGCCTGGGGAATGATTCA GAAGAAAAACGTGGTTTGGAAGCTGCAGAGGTGAACCCAGAGAGCCTGGCAAAAGAATGTATTCAAAAAA GTCTGTTATTACTGAAATTTTTGCCCATGAGCAAAAGTTCAAAAGAAAACTGTGACAAGTTGGAAACTGT CGATGAAACTGATCATCTCCAGCCACTTGATAGACGCCAGAGGACAAGCTCTGTGGTGGAAGAACATTTC CAGGGTTCAGCATCTCCTACAGAAGCAGCCACCCCAGCTGCAGGTGACAGGAGTCCTGCCTTAGAGATCC AGCCAAAGCTACTACCCAGTAGTGGCCCCTGTGTTGCAGAAGTGTCCACAGCTGAAGAGCCCTCACCACC ATCCACACCCACCCGGAGGCCTCCTTTCACCCGAGGGCGACTCCGGCTGCTCTCTTTCAGATCCATGGAA GAGACCAGGCCAGTCCCCACAGTCAAGGAGAAATACCCGGTGCTGAAGGATGTCATGGACTTCATTAAAG ACCAGTCACTCTCACACGAGAGTGTTGTGAAGGTTCTTTCTTTGCGGAAAGCCCAAGGGCAGAGCATACT TGAAGTCCTGAGGATAATTCAGTATTGCACAGAATCCCTCGGGCAGCCTCACTGCTTCCACCCACCGTAT ATACTTTTCCTACTGGAACTTCTGACCTGCCAGAAAGATTTTACAAATTACTTTGGACACTTGGAAGGCT GTGGTGCTGATCTACACAGAGAAATCCGAGACACTTACTATCAACTTGTTCTGTTTTTGGTCAAAGCAAT CAAAAGATTTAGTAGCATAAATGACAGGTCTCTGCTCCCTGCTTTATCTTGTGTCCAGACAGCCCTGCTT CATCTTTTGGATATGGGCTGGGAACCCAGTGATCTTGCCTTCTTTGTTGACATTCAGTTACCAGATCTCC TCATGAACATGTCCCAAGAAAACATCAGTGTCCATGACAGTGTGATTAGCCAGTGGAGTGAAGAAGATGA ACTCGCTGATGCCAAGCAGAACTCAGAGTGGATGGATGAGTGTCAGGATGGCATGTTTGAGGCCTGGTAT GAAAAAATAGCCCAAGAGGATCCAGAGAAGCAGAGGAAAATGCACATGTTTATTGCTCGATACTGTGACC TGTTAAATGTGGATATCTCTTGTGATGGGTGTGATGAGATTGCCCCCTGGCATCGATACCGATGTCTACA GTGCAGTGACATGGACCTCTGCAAGACTTGCTTCCTTGGTGGAGTGAAGCCCGAGGGCCATGGAGACGAT CATGAAATGGTTAACATGGAGTTTACGTGTGACCACTGCCAGGGTTTGATCATAGGCCGGAGAATGAATT GCAATGTATGTGATGACTTTGATCTTTGCTATGGATGCTACACAGCAAAGAAATACTCCTATGGCCATTT GCCAACTCACAGCATCACAGCCCATCCAATGGTGACCATCCGCATCAGTGACCGGCAGAGGCTCATCCAG CCCTATATCCACAACTACTCCTGGCTGCTCTTTGCTGCCCTGGCTCTCTATAGTGCCCACCTGACCAGTA CAGAGCAGGTGGATGGGGAGCAGCTGGACCCCCAGGCCCGCACCAATGCTGCCACCCTGAGGAGCCAGTG CATGCAGCTGGTTGGGGACTGTCTGATGAAGGCCCACCAGGGCAAAGGTCTTAAAGCTCTGGCTCTTCTT GGTGTATTGCCAGATGGTGACTCTACCTCAGAAAATCAAGCCCTGCCAGTCACAGTGTCCTTCCAGGCAT CAGAGGAGCAGGCAGATGCAGGCCTGTTGGTACCCTGCAACGGAAAGAGAGCTGCAGATACGGAAGTAAG ACCCTTGGATTATAAGCAGAAGAAAAAGGCAGGTGAAGATCTCTCCATAGTGAAGGACCCTTCATGCCAG ACCCAGGTTTCAGATGCACCTGCCAGTGCTCACGTGCCTCCAGGACTTCCAGATGCTGAACATCCAGAAG TGTCAGCTCAGGTACTGGTAGAAGAGAAGGCGATTACTCCAAACCCTGAGCAAGTGTTTGCAGAGTGTTC CCAGAAGAGGATTTTGGGATTGCTGGCAGCCATGTTACCTCCCATCAAGTCGGGTCCCACAGTCCCCCTG ATAGATCTGGAGCACGTCCTCCCACTCATGTTTCAGGTGGTCATCTCCAACGCAGGCCACCTGAATGAAA CCTACCACCTCACCCTGGGTCTTCTGGGCCAGTTAATTATCCGTCTTCAGCCAGCAGAAGTAGATGCTGC AGTCATGAAGGTCCTTTCAGCCAAGCACAACCTCCGAGTGGGCCTGGACTGGGCCTGCTCCATGGCAGAG ATCCTTCGGTCACTCAACAATGCCCCGTTGTGGCGAGATGTCATTGCCACCTTCACAGACCACTGCATCA AGCAGCTGCCATTCCAACTGAAGCACACCAACATCTTCACTCTGCTGGTGCTTGTCGGCTTCCCCCAGGT CCTCTGCGTGGGAACCCGCTGTGTTTATATGGATAATGCCAATGAACCCCATAATGTGATCATCTTGAAA CACTTCACTGAGAAGAATAGAGCTGTGATTGTCGACGTCAAAACAAGGAAAAGGAAAACAGTGAAGGACT ATCAGCTTGTCCAGAAGGGAGGAGGACAAGAGTGTGGCACCTCTCAGTCCCAGCTGAGCCAGTACTCCCA GCACTTTGCCTTTATTGCTAGTCACCTTCTGCAAACCAGCATGGATAGCCATTGTCCCGAGGCAGTGGAA GCAACCTGGGTCCTGTCCCTGGCTCTCAAAGGTTTATATAAAACACTAAAGGCTCATGGTTTTGAAGAGA CCCATGCCACCTTCCTCCAGACCGATCTGCTAAAGCTGCTGGTGAAGAAGTGCAGCAAAGGGACCGGCTT CAGTAAAACCTGGCTCCTCCGAGACCTAGAGATTCTGTCCATCATGCTGTACTCGTCAAAAAAGGAGATC AACACTCTGGCTGAACATGGAGACCTGGAGCTAGATGAACGAGGAGACCAAGAAGAAGAACTGGACCGGC CAGTTAGTAGCCCTGGCGAAGCAGAACAGAAGAAGCTAGACCCTCTTGAAAATTTGGATGAACCCACCAG AATATGCTTCTTGATGGCTCATGATGCCCTCAATGCCCCTCTGCACATTCTCCGGGCCATATATGAACTG CAGATGAAAAAGACTGATTCGTTCTTCCTAGAGGTTCAGAAGAGGTTTGATGGGGATGAGCTCACTACAG ATGAGAGGATTCGCTCCTTGGCCCAGAGGTGGCAACCCAGCAGGAGTCTGAGGCTGGAGGAGCAGAGCGC CAAAGCTGTGGACACAGACATGATTATCTTGCCATGCTTGTCCCGGCCTGCACGCTCTGACCAAGCCACT CCTGAATCAAATCCTGTGACCCAGAAGCTGATTTCCAGCACAGAGAGCGAGCTACAGCAGAGCTATGCCA AGCAGCGCCGGAGCAAGAGTGCTGCCCTCCTGCACAAGGAGCTGAACTGCAAGAGCAAGCGGGCTATCCG GGACTACCTCTTCCGTGTGAACGAGGCCACCTCAGTCCTATATGCTCGCCATGTGCTCGCCTCCCTGCTG GCCGAGTGGCCAGGGCATGTGCCAGTGAGTGAGGACATCCTGGAGCTTAGTGGCCCTGCACATATGACCT ACATTCTGGATATGTTCATGCAGCTGGAAGAAAAGCACCAGTGGGAGAAGATCCTACAGAAAGTACTCCA GGGCTGCCGAGAGAACATGCTGGGCACCATGGCCTTGGCTGCCTGTCAGTTCATGGAGGAACCAGGAATG GAGGTGCAATGCAACACTGAGGAAGGCTGTGATGAACTAGCCATGTCCAGCAGTAGTGACTTTCAGCAGG ACCGGCACAACTTCAGCGGGTCGCAACAGAAGTGGAAAGATTTTGAGCTTCCAGGAGATACTCTGTATTA CCGATTTACCTCGGACATGAGCAACACTGAGTGGGGATACCGATTCACTGTGACAGCTGGACATCTGGGG CGGTTCCAGACAGGGTTTGAGATCTTGAAGCAGATGTTGTCTGAAGAAAGGGTTGTACCTCACCTCGCAT TGGGCAAGATCTGGGAATGGCTGGTGGGTGTGGCCTGCCGCCAAACCGGTCATCAGCGCCTGAAAGCCAT CCACTTACTACTGAGGATTGTGCAATGCTGCAGCCACAGTGACCTCTGTGATCTTGGGCTGTTGAAGCCC CTGTGGCAGCTCTTTACTCACATGGAGTATGGCCTGTTTGAGGATGTGACACAGCCTGGCATTCTCCTTC CCCTACACCGAGCCCTTACTGAGCTCTTCTTTGTCACCGAGAACCGTGCCCAGGAGCTTGGCCTGCTTCA GGAGTACCTGCTGGCCTTAACCACCGAGGACCACCTTCTCCGATGTGCAGCACAGGCTCTGCAGAACATT GCTGCTATCAGCCTGGCCATCAACTACCCAAACAAGGCCACCCGCCTCTGGAATGTTGAGTGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001045536
Insert Size	8676 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001045536.2, NP_001039001.1
RefSeq Size	11150 bp
RefSeq ORF	8676 bp
Locus ID	195018
UniProt ID	Q5SSH7

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.