Apc (NM_007462) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI047805; AU020952; AW124434; CC1; mAPC; Min
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225418 representing NM_007462 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCTTCATATGATCAGTTGTTAAAGCAAGTTGAGGCACTGAAGATGGAGAACTCAAATCTTC GACAAGAGCTAGAAGATAATTCCAATCATCTTACAAAACTGGAAACTGAGGCATCTAATATGAAGGAAGT ACTTAAGCAGCTACAGGGAAGTATTGAAGATGAGACTATGACTTCTGGACAGATTGACTTACTAGAGCGT CTTAAAGAATTTAACTTAGATAGTAATTTCCCCGGAGTGAAACTACGCTCAAAAATGTCCCTTCGCTCCT ACGGAAGTCGGGAAGGATCTGTATCCAGCCGTTCAGGAGAATGCAGTCCTGTCCCCATGGGGTCATTCCC AAGAAGAACATTTGTAAATGGAAGCAGAGAGAGTACTGGGTATCTAGAAGAGCTTGAAAAAGAAAGATCA TTACTCCTTGCTGATCTTGACAAAGAAGAGAAGGAAAAGGACTGGTATTATGCTCAACTTCAGAACCTCA CAAAAAGAATAGATAGCCTGCCTTTAACTGAAAATTTTTCCTTACAGACAGACATGACAAGACGGCAGCT GGAGTATGAAGCAAGGCAGATCAGGGCTGCAATGGAGGAGCAGCTTGGCACCTGCCAGGACATGGAGAAG CGTGCACAGCGAAGAATAGCCAGGATCCAGCAAATAGAAAAGGACATACTGCGCGTGCGCCAGCTTTTAC AGTCCCAGGCGGCGGAAGCGGAGAGGTCATCTCAGAGCAGGCATGATGCTGCCTCCCATGAAGCTGGCCG GCAGCACGAAGGCCACGGAGTGGCAGAAAGCAACACCGCAGCCTCCAGTAGTGGTCAGAGTCCAGCTACA CGTGTGGATCACGAAACAGCCAGTGTTTTGAGTTCTAGCGGCACGCACTCTGCTCCTCGAAGGTTGACAA GTCATCTGGGGACAAAGGTGGAAATGGTGTATTCCTTGTTGTCAATGCTTGGTACTCATGATAAGGACGA TATGTCACGAACTTTGCTAGCTATGTCCAGCTCCCAAGACAGCTGTATATCCATGCGGCAGTCTGGATGT CTTCCTCTCCTCATCCAGCTTTTACATGGCAATGACAAAGACTCTGTATTGTTGGGAAATTCCCGGGGCA GTAAAGAGGCTCGGGCCAGGGCCAGTGCAGCACTCCACAACATCATTCACTCACAGCCTGATGACAAGAG AGGCAGGCGTGAAATCCGAGTCCTTCATCTTTTGGAACAGATACGAGCTTACTGTGAAACCTGTTGGGAG TGGCAGGAAGCCCACGAACAAGGCATGGACCAGGACAAAAACCCAATGCCAGCTCCTGTTGAGCATCAGA TCTGTCCTGCTGTGTGTGTTCTAATGAAGCTTTCATTTGATGAAGAGCATAGGCATGCAATGAATGAACT TGGGGGACTGCAGGCCATTGCAGAGTTATTGCAGGTGGACTGTGAGATGTATGGGCTTACTAATGACCAC TACAGTGTTACTTTAAGACGGTATGCTGGAATGGCTTTGACAAACTTGACCTTTGGAGATGTTGCCAACA AGGCTACGCTGTGTTCTATGAAAGGCTGCATGAGAGCACTTGTGGCCCAGTTAAAATCTGAGAGTGAAGA CTTACAGCAGGTTATTGCAAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAATAGCAAAAAGACG TTGAGAGAAGTTGGAAGTGTGAAAGCATTGATGGAATGTGCTTTGGAAGTTAAAAAGGAATCAACCCTCA AAAGCGTTTTGAGTGCCTTATGGAACCTGTCTGCACACTGCACTGAGAATAAGGCTGACATCTGTGCTGT GGATGGAGCACTGGCATTTCTGGTTGGCACCCTCACTTACCGGAGCCAGACAAATACTTTAGCCATTATT GAAAGTGGAGGTGGGATATTACGGAATGTGTCCAGCTTGATAGCTACAAACGAAGACCACAGGCAAATCC TAAGAGAGAACAATTGCCTACAAACTTTATTACAGCACTTGAAATCTCACAGCTTGACAATAGTCAGTAA TGCATGTGGAACTTTGTGGAATCTCTCAGCAAGAAATCCTAAAGACCAGGAAGCCTTGTGGGACATGGGG GCAGTGAGCATGCTCAAGAACCTCATTCATTCCAAGCACAAAATGATTGCCATGGGAAGTGCAGCAGCTT TAAGGAATCTCATGGCAAACAGACCTGCAAAGTATAAGGATGCCAATATCATGTCTCCCGGCTCAAGTCT GCCATCCCTTCACGTTAGGAAACAGAAAGCTCTAGAAGCTGAGCTAGATGCTCAGCATTTATCAGAAACC TTCGACAACATTGACAACCTAAGTCCCAAGGCCTCTCACCGGAGTAAGCAGAGACACAAGCAGAATCTTT ATGGTGACTATGCTTTTGACGCCAATCGACATGATGATAGTAGGTCAGACAATTTCAATACTGGAAACAT GACTGTTCTTTCACCATATTTAAATACTACGGTATTGCCCAGCTCTTCTTCCTCAAGGGGAAGTTTAGAC AGTTCTCGTTCTGAGAAAGACAGAAGTTTGGAGAGAGAGCGAGGTATTGGCCTCAGTGCTTACCATCCAA CAACAGAAAATGCAGGAACCTCATCAAAACGAGGTCTGCAGATCACTACCACTGCAGCCCAGATAGCCAA AGTTATGGAAGAAGTATCAGCCATTCATACCTCCCAGGACGACAGAAGTTCTGCTTCTACCACCGAGTTC CATTGTGTGGCAGACGACAGGAGTGCGGCACGAAGAAGCTCTGCCTCCCACACACACTCAAACACATACA ACTTCACTAAGTCGGAAAATTCAAATAGGACATGCTCTATGCCTTATGCCAAAGTGGAATATAAACGATC TTCAAATGACAGTTTAAATAGTGTCACTAGTAGTGATGGATATGGTAAAAGAGGCCAAATGAAACCCTCA GTTGAATCCTATTCTGAAGATGATGAAAGTAAATTTTGCAGTTATGGTCAGTATCCAGCTGACCTAGCCC ATAAGATACACAGTGCAAATCATATGGATGATAATGATGGAGAACTGGATACACCAATAAATTACAGTCT TAAATATTCAGATGAGCAGTTGAACTCAGGAAGGCAGAGTCCCTCACAGAATGAAAGGTGGGCAAGACCA AAGCATGTGATAGAAGATGAAATAAAGCAAAACGAGCAAAGACAAGCAAGAAGCCAGAACACCAGTTATC CTGTCTATTCTGAGAATACCGATGACAAACACCTCAAATTCCAACCACATTTTGGACAACAAGAATGTGT TTCCCCATATAGGTCAAGGGGAACCAGTGGTTCAGAAACAAATCGAATGGGTTCTAGTCATGCAATTAAT CAAAATGTAAACCAGTCTCTGTGTCAGGAAGATGATTATGAAGATGATAAACCTACCAACTACAGTGAAC GTTATTCTGAGGAAGAACAACATGAAGAAGAAGAAGAGAGACCGACAAATTATAGCATAAAATATAATGA AGAGAAACATCATGTGGATCAGCCTATTGATTATAGTTTAAAATATGCCACTGACATTTCTTCCTCACAA AAACCATCATTTTCATTCTCAAAGAATTCATCAGCACAAAGCACTAAACCTGAACATCTCTCTCCAAGCA GCGAGAATACAGCTGTACCTCCATCTAATGCCAAAAGGCAGAATCAGCTGCGTCCAAGTTCAGCACAAAG AAATGGCCAGACTCAAAAAGGCACTACTTGCAAAGTCCCCTCCATCAACCAAGAAACAATACAGACTTAC TGCGTAGAAGACACCCCAATATGTTTTTCAAGGTGCAGTTCATTATCATCACTGTCATCAGCTGACGATG AAATAGGATGTGATCAGACAACACAGGAAGCAGATTCTGCTAATACTCTGCAGACAGCAGAAGTAAAAGA GAATGATGTAACTCGGTCAGCTGAAGATCCTGCAACTGAAGTTCCAGCAGTGTCCCAGAATGCTAGAGCC AAACCCAGCCGACTCCAGGCTTCTGGCTTATCTTCAGAATCAACCAGGCATAATAAAGCTGTTGAGTTTT CTTCAGGAGCCAAGTCTCCCTCCAAAAGTGGTGCTCAGACACCCAAAAGTCCCCCAGAACACTATGTCCA GGAGACTCCGCTCGTATTCAGCAGGTGTACTTCTGTCAGCTCCCTTGACAGTTTTGAGAGTCGCTCCATT GCCAGCTCTGTTCAGAGTGAGCCATGTAGTGGAATGGTGAGTGGCATCATAAGCCCCAGTGACCTTCCAG ATAGTCCTGGGCAGACCATGCCACCAAGCAGAAGCAAAACCCCTCCACCTCCTCCACAGACAGTGCAGGC CAAGAGAGAGGTGCCAAAAAGTAAAGTCCCTGCTGCTGAGAAGAGAGAGAGTGGGCCTAAGCAGACTGCT GTAAATGCTGCCGTGCAGAGGGTGCAGGTCCTTCCAGACGTGGATACTTTGTTACACTTCGCCACAGAAA GTACTCCAGACGGGTTTTCTTGTTCCTCCAGCCTAAGTGCTCTGAGCCTGGATGAGCCATTTATACAGAA AGATGTAGAATTAAGAATCATGCCTCCAGTTCAGGAAAACGACAATGGGAATGAAACTGAATCAGAACAG CCTGAGGAATCAAATGAAAACCAGGATAAAGAGGTAGAAAAGCCTGACTCTGAAAAAGACTTATTAGATG ATTCTGATGACGATGATATTGAAATATTAGAAGAATGTATTATTTCAGCCATGCCAACAAAGTCATCACG CAAAGCCAAAAAACTAGCCCAGACTGCTTCAAAATTACCTCCACCTGTGGCAAGGAAACCAAGTCAGCTA CCTGTGTATAAACTTCTGCCAGCACAGAATAGGCTGCAGGCACAAAAACATGTTAGCTTTACACCAGGGG ATGATGTGCCCCGGGTGTACTGTGTAGAAGGGACACCTATAAACTTTTCCACAGCAACGTCTCTAAGTGA TCTGACAATAGAGTCCCCTCCAAATGAATTGGCTACTGGAGATGGGGTCAGAGCGGGTATACAGTCAGGT GAATTTGAAAAACGAGATACCATTCCTACAGAAGGCAGAAGTACAGATGATGCTCAGCGAGGAAAAATCT CATCTATAGTTACACCAGACCTGGATGACAACAAAGCAGAGGAAGGAGATATTCTTGCAGAATGTATCAA TTCTGCTATGCCCAAAGGAAAAAGCCACAAGCCTTTCCGAGTGAAAAAGATAATGGACCAAGTCCAACAA GCATCCTCGACTTCATCTGGAGCTAACAAAAATCAAGTAGACACTAAGAAAAAGAAGCCTACTTCACCAG TAAAGCCCATGCCACAAAATACTGAATATAGAACGCGTGTGAGAAAGAATACAGACTCAAAAGTTAATGT AAATACTGAAGAAACTTTCTCAGACAACAAAGACTCAAAGAAACCAAGCTTACAAACCAATGCCAAGGCC TTCAATGAAAAGCTACCTAACAATGAAGACAGAGTGCGGGGGAGCTTCGCCTTGGACTCACCGCATCACT ACACCCCTATTGAGGGGACGCCGTACTGCTTTTCCCGAAATGACTCCTTGAGTTCTCTGGATTTTGATGA TGACGATGTTGACCTTTCCAGGGAAAAGGCCGAGTTAAGAAAGGGCAAAGAAAGCAAGGATTCCGAAGCC AAAGTTACCTGCCGCCCAGAACCAAACTCAAGCCAGCAGGCAGCTAGTAAGTCACAAGCCAGTATAAAAC ATCCAGCAAACAGAGCACAGTCCAAACCAGTGCTGCAGAAACAGCCCACTTTCCCCCAGTCCTCCAAAGA CGGACCAGATAGAGGGGCAGCAACTGACGAAAAACTGCAGAATTTTGCTATTGAAAATACTCCAGTTTGC TTTTCTCGAAATTCCTCTCTGAGTTCCCTTAGTGACATTGACCAGGAAAACAACAATAACAAAGAAAGTG AACCAATCAAAGAAGCTGAACCTGCCAACTCACAAGGAGAGCCCAGTAAGCCTCAGGCATCCGGGTATGC TCCCAAGTCCTTCCACGTCGAAGACACCCCTGTCTGTTTCTCAAGAAACAGCTCTCTCAGTTCTCTTAGC ATTGACTCTGAGGACGACCTGTTACAGGAGTGTATAAGTTCTGCCATGCCAAAAAAGAAAAGGCCTTCAA GACTCAAGAGTGAGAGCGAAAAGCAGAGCCCTAGAAAAGTGGGTGGCATATTAGCTGAAGACCTGACGCT TGATTTGAAAGATCTACAGAGGCCAGATTCAGAACACGCTTTCTCCCCCGACTCAGAAAATTTTGACTGG AAAGCTATTCAGGAAGGCGCAAACTCCATAGTAAGTAGTTTGCACCAAGCTGCTGCAGCCGCCGCGTGCT TATCTAGACAAGCGTCATCCGACTCAGATTCCATTCTGTCACTAAAGTCCGGCATTTCTCTGGGATCGCC TTTTCATCTTACACCTGATCAAGAGGAAAAGCCATTCACAAGCAATAAAGGCCCAAGAATTCTCAAACCT GGAGAGAAAAGCACATTAGAAGCAAAAAAAATAGAATCTGAAAACAAAGGAATCAAAGGCGGGAAAAAGG TTTATAAAAGCTTGATTACGGGAAAGATTCGCTCCAATTCAGAAATTTCCAGCCAAATGAAACAACCCCT CCCGACAAACATGCCTTCAATCTCAAGAGGCAGGACGATGATTCACATCCCAGGGCTTCGGAATAGCTCC TCTAGTACAAGCCCTGTCTCTAAGAAAGGCCCACCCCTCAAGACTCCAGCCTCTAAAAGCCCCAGTGAAG GGCCGGGAGCTACCACTTCTCCTCGAGGAACTAAGCCAGCAGGAAAGTCAGAGCTTAGCCCTATCACCAG GCAAACTTCCCAAATCAGTGGGTCAAATAAGGGGTCTTCTAGATCAGGATCTAGAGACTCCACTCCCTCA AGACCTACACAGCAACCATTAAGTAGGCCAATGCAGTCTCCAGGGCGAAACTCAATTTCCCCTGGTAGAA ATGGAATAAGCCCTCCTAACAAACTGTCTCAGCTGCCCAGAACATCATCTCCCAGTACTGCTTCAACTAA GTCCTCCGGTTCTGGGAAAATGTCATATACATCCCCAGGTAGACAGCTGAGCCAACAAAATCTTACCAAA CAAGCAAGTTTATCCAAGAATGCCAGCAGTATCCCCAGAAGTGAGTCGGCATCTAAAGGACTGAATCAGA TGAGTAACGGCAATGGGTCAAATAAAAAGGTAGAACTTTCTAGAATGTCTTCAACTAAATCAAGTGGAAG TGAATCAGACAGATCAGAAAGGCCTGCATTAGTACGCCAGTCTACTTTCATCAAAGAAGCCCCAAGCCCA ACCCTGAGGAGGAAACTGGAGGAATCTGCCTCATTTGAATCCCTTTCTCCATCTTCTAGACCAGATTCTC CCACCAGGTCGCAGGCACAGACCCCAGTTTTAAGCCCTTCCCTTCCTGATATGTCTCTGTCCACACATCC ATCTGTTCAGGCAGGTGGGTGGCGAAAGCTCCCGCCTAATCTCAGCCCCACTATCGAGTATAATGACGGA AGGCCCACAAAACGGCATGATATTGCACGCTCCCATTCTGAAAGTCCTTCCAGACTACCAATCAACCGGG CGGGAACCTGGAAGCGTGAACACAGCAAACATTCCTCGTCCCTTCCTCGAGTGAGTACTTGGAGAAGAAC TGGAAGCTCATCTTCTATTCTTTCTGCTTCATCAGAGTCCAGTGAAAAAGCAAAAAGTGAGGATGAAAGG CATGTGAGCTCCATGCCAGCACCCAGACAGATGAAGGAAAACCAGGTGCCCACCAAAGGAACATGGAGGA AAATCAAGGAAAGTGACATTTCTCCCACAGGCATGGCTTCTCAGAGCGCTTCCTCAGGTGCTGCCAGTGG TGCTGAATCCAAGCCTCTGATCTATCAGATGGCACCTCCTGTCTCTAAAACAGAGGATGTTTGGGTGAGA ATTGAGGACTGCCCCATTAACAACCCTAGATCTGGACGGTCCCCCACAGGCAACACCCCCCCAGTGATTG ACAGTGTTTCAGAGAAGGGAAGTTCAAGCATTAAAGATTCAAAAGACACCCATGGGAAACAGAGTGTGGG CAGTGGCAGTCCTGTGCAAACCGTGGGTCTGGAAACCCGCCTCAACTCCTTTGTTCAGGTAGAGGCCCCA GAACAGAAAGGAACTGAGGCAAAACCAGGACAGAGTAACCCAGTCTCTATAGCAGAGACTGCTGAGACGT GTATAGCAGAGCGTACCCCTTTCAGTTCCAGTAGCTCCAGCAAGCACAGCTCACCTAGCGGGACTGTTGC TGCCAGAGTGACACCTTTTAATTACAACCCTAGCCCTAGGAAGAGCAGCGCAGACAGCACTTCAGCCCGG CCGTCTCAGATCCCTACGCCAGTGAGCACCAACACGAAGAAGAGAGATTCGAAGACTGACAGCACAGAAT CCAGTGGAGCCCAAAGTCCTAAACGCCATTCCGGGTCTTACCTCGTGACGTCTGTTTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_007462
Insert Size	8529 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007462.3, NP_031488.2
RefSeq Size	12453 bp
RefSeq ORF	8529 bp
Locus ID	11789

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