Mga (NM_001164274) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV312082; C80739; C130042M01Rik; D030062C11Rik; Mad5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225417 representing NM_001164274 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGAAACAGCAAATCATATTGGCCAATCAAGATGGAGGGACAGTGACAGGAGGAGCACCTACCT TCTTTGTCATCCTAAAGCAGCCAGGAAATGGCAAAACTGATCAAGGAATTTTAGTTACTAATCGAGATGC CCGTGCTTTGTTGAGTAGGGAGTCATCACCAGGAAAATCTAAAGAGAAAATTTGCCTTCCGGCTGATTGT ACTGTGGGAAAAATCACGGTTACCCTTGATAACAATAGTATGTGGAATGAGTTCCATAATCGAAGCACAG AGATGATTCTGACCAAACAGGGAAGACGCATGTTTCCTTATTGTCGATATTGGATAACAGGCTTAGATTC AAATTTAAAGTATATCCTTGTAATGGACATATCTCCTGTGGATAGTCATCGGTATAAGTGGAATGGTCGT TGGTGGGAACCCAGTGGGAAGGCTGAACCCCATATTTTGGGGAGAGTTTTCATTCATCCAGAATCTCCTT CTACAGGTCATTATTGGATGCATCAACCAGTGTCTTTCTATAAACTCAAACTTACTAACAATACACTGGA CCAGGAAGGACATATTATCTTGCACTCTATGCATCGTTATCTGCCAAGGCTTCATTTGGTGCCAGCAGAA AAGGCTACAGAAGTGATACAGTTAAATGGCCCCGGTGTTCATACTTTTACTTTCCCCCAGACTGAATTCT TTGCAGTAACAGCTTATCAGAACATTCAGATTACTCAGTTGAAAATAGATTACAATCCTTTTGCCAAAGG CTTTCGGGATGATGGGTTGAGCAGTAAGCCTCAGAGAGAAGGAAAACAGAGGAATAGCTCCGATCAAGAA GGGAATAGTGTTTCTAGTTCTCCTGCTCATCGTGTTCGTCTTACAGAAGGTGAAGGGTCAGAGATACATT CAGGTGATTTTGATCCTGTGTTAAGGGGTCATGAAGCATCAAGCTTAAGTTTAGAGAAGGCTCCCCATAA TGTAAAACAAGACTTTCTTGGATTTATGAATACTGATTCGACACATGAAGTTCCTCAGTTGAAACATGAG ATTTCTGAAAGTCGTATTGTGAACAGTTTTGAAGATGATTCCCAGATTTCCTCACCATCAAACCCGAATG GAAACTTTAATGTCGTCATTAAAGAAGAGCCTCTAGATGATTATGATTATGAACTTGGTGAATGCCCAGA AGGGATAACAGTGAAACAAGAAGAGACAGATGAGGAAACGGATGTATACTCAAACAGCGATGACGATCCT ATACTAGAGAAACAACTAAAGAGGCACAATAAAGTTGATAATTTAGAAGCTGACCATCCATCTTATAAAT GGCTACCAAATAGCCCAGGTGTTGCTAAAGCTAAAATGTTTAAATTAGATGCTGGGAAAATGCCAGTAGT TTACTTGGAGCCCTGTGCTGTCACGAAAAGCACAGTGAAAATTTCTGAATTGCCTGATAATATGCTCTCC ACTTCTCGAAAAGATAAATCTATGTTAGCAGAATTAGAATATTTGCCTGCGTATATTGAAAATTCTGATG GGACTGACTTTTGCTTAAGCAAGGATTCAGAAAATAGTCTCAGAAAACATTCACCAGATCTCAGAATTGT ACAAAAATATACTTTACTTAAAGAGCCTAATTGGAAATACCCGGACATACTTGACAACAGTAGCACGGAA AGAATACATGACAGTTCCAAAGGATCAACTGCAGAGTCATTTTCAGGAAAAGAGGACTTAGGCAAAAAGC GAACAACAATGCTTAAAATGGCAATACCATCAAAGACTGTGACTGCTAGTCATAGTGCCTCTCCAAATAC TCCTGGGAAAAGAGGAAGACCGAGAAAGTTGAGACTCTCTAAGGCAGGGCGACCACCTAAAAACACAGGG AAATCTTTAACTGCTGCCAAGAATATTCCTGTAGGCCCTGGAAGCACCTTTCCTGATGTGAAACCTGATC TAGAAGATGTGGATGGGGTGCTCTTTGTTTCCTTTGAATCAAAGGAGGCTCTTGATATTCATGCAGTTGA TGGGACAACAGAAGAACCTTCTAGTCTTCAGACCACAACCACAAATGATTCAGGTTGCAGAACAAGAATT TCCCAGTTGGAAAAGGAATTAATAGAAGATTTGAAGTCTTTGAGGCATAAGCAGGTGATACATCCTGCTC TTCAAGAAGTGGGCTTAAAACTGAATTCAGTGGATCCAACAGTGAGCATTGATCTTAAATACTTGGGAGT ACAATTGCCTTTGGCTCCAGCCACCAGCTTTCCCCTATGGAATGTTACAGGTACCAACCCTGCCTCACCT GATGCTGGGTTTCCTTTTGTTTCTAGGACGGGGAAGACCAATGACTTTACCAAAATCAAGGGATGGAGAG GAAAATTTCAAAATGCTTCTGCATCTAGGAATGAAGGTGGAAATTCAGAGGCTTCACTGAAAAACCGTTC TGCTTTCTGTAGTGATAAGCTAGATGAGTACTTGGAAAATGAAGGCAAATTGATGGAAACAAACATAGGT TTCTCTTCAAATGCTCCCACATCTCCAGTAGTGTACCAGCTACCCACCAAGAGTACCAGCTATGTTCGAA CTCTTGATAGTGTACTAAAGAAGCAATCTACCATTTCCCCTTCTACCTCTCACTCTGTGAAGCCTCAGTC TGTAACCACTGCCTCTCGAAAAACAAAGGCTCAGAACAAACAGACAACACTCAGTGGCCGAACTAAATCA TCTTATAAGTCTATTTTACCATACCCTGTTTCACCAAAGCAGAAAAACTCTCATGTGAGCCAAGGAGATA AAATTACCAAGAATTCCTTGAGTTCGACCTCAGATAATCAGGTGACTAACTTGGTTGTGCCATCTGTAGA TGAAAATGCATTTCCAAAGCAGATTAGTTTGCGGCAGGCCCAGCAACAGCACCTTCAGCAGCAAGGAACT CGCCCTCCAGGCTTGTCGAAATCTCAAGTAAAGCTTATGGACCTGGAAGACTGTGCACTCTGGGAAGGAA AACCAAGGACCTATATTACTGAAGAGCGAGCAGATGTCTCCTTGACAACTCTGCTTACTGCTCAGGCATC TCTCAAAACTAAACCTATCCACACAATCATAAGGAAACGAGCTCCTCCATGCAACAATGACTTCTGTCGC CTGGGTTGTGTGTGCTCTAGTCTAGCTTTGGAAAAGCGCCAACCTGCTCACTGCCGTCGACCAGACTGCA TGTTTGGTTGCACTTGTTTGAAAAGAAAAGTTGTGTTGGTTAAAGGGGGATCTAAAACAAAGCATTTCCA TAAGAAGGCTGCTAATCGAGATCCATTATTTTATGATACGCTGGGAGAGGAAGGAAGGGAAGGAGGAGGA GTCAGAGAAGATGAGGAACAATTGAAGGAGAAAAAGAAGAGAAAGAAGCTCGAATACACTGTATGTGAAG CAGAACCTGAGCAGCCGGTTCGACATTACCCACTGTGGGTGAAGGTAGAAGGTGAAGTAGATCCAGAGCC GGTTTATATTCCAACTCCTTCTGTCATTGAACCTATAAAACCATTGGTGTTGCCTCAGCCGGATTTATCT TCTACTACGAAGGGCAAACTAACCCCTGGAATTAAACCAGCACGAACATATACTCCCAAGCCCAATCCTA TACGAGAAGAGGACAAAGATCCAGTCTATTTGTACTTTGAAAGTATGATGACTTGTGCCCGAGTTCGAGT ATATGAACGAAAGAAAGAGGAACAGAGACAACTGTCTCCACCCCTGTCTCCATCCTCATCATTTCAGCAG CAGAGTTCCTGTTACTCTAGTCCTGAGAACCGTGTTACAAAGGAACTTGATTCTGAACAGACCTTAAAGC AGCTCATTTGTGACTTGGAGGATGATTCTGATAAATCACAAGAAAAGAGCTGGAAATCCTCCTGCAATGA AGGAGAGTCCTCTTCTACCTCGTATGTACATCAGAGGTCACCTGGTGGTCCTACCAAATTGATAGAGATC ATCTCAGACTGCAACTGGGAAGAAGATCGAAACAAGATCTTGAGCATTTTATCTCAGCACATCAATAGCA ACATGCCACAGTCACTTAAGGTGGGCAGCTTCATCATTGAGTTGGCTTCTCAGCGGAAGTGTCGGGGTGA GAAGACCCCTCCTGTTTATTCTTCTCGTGTGAAAATCTCTATGCCATCAAGTCAAGACCAAGATGATATG GCTGAGAAATCTGGATCAGAGACTCCTGATGGTCCATTGTCCCCTGGGAAAATGGATGATATCTCTCCTG TGCAGACAGATGCCCTGGATTCAGTGAGGGAGAGATTACATGGAGGCAAAGGTCTACCTTTTTATGCAGG GCTTTCTCCTTCGGGGAAGCTTGTGGCCTATAAACGTAAACCCAGTTCAACTACATCTGGGCTTATCCAG GTAGCATCCAATGCCAAGGTGGCTGCATCCAGGAAACCACGCACCCTGTTGCCTTCAACATCCAATTCCA AAATGGCATCCTCTGGCCCAGCAACAAATCGCTCTGGGAAGAATCTGAAGGCATTTGTTCCAGCAAAAAG GCCAATTGAAAATGCCCCTCAAATTCCAGTGACTACTCCACAGATCTCTTCTAACAACGTGAAACGTACT GGACCTCGATTGTTGTTGATTCCAGTGCAGCAGGGTTCTCCTACGCTTAGACCAATCCAAAACCCACAGC TTCAGGGACAGCGGATGGTCTTGCAACCTGTTAGGGGTCCAAGTGGAATGAACCTATTCAGGCACCCCAA TGGGCAGATTGTCCAACTCCTACCTTTACATCAGATTCGAGGCTCTAATGCCCAGCCCAGCTTACAGCCT GTGGTATTTCGGAACCCAGGATCTATGGTGGGAATCCGACTACCAGCTCCTTGCAAATCTTCAGAGACTC CATCATCTTCTGCTTCATCCTCTGCCTTCTCTGTCATGAGTCCTGTGATTCAGGCTGTTGGGTCTTCTCC AACAGTAAATGTCATTTCTCAGGCACCTTCACTGCTTTCCTCTGGATCTAGTTTTGTCTCTCAGGCTGGT ACACTGACTCTGAGGATCTCTCCTCCTGAAACTCAAAACCTTGCAAGTAAAACAGGCTCTGAAAGCAAAA TAACACCCAGCACTGGAGGACAGCCTGTAGGCACTGCTAGCCTCATTCCTCTGCAGTCAGGTAGTTTTGC CTTGTTGCAGCTCCCAGGACAAAAGCCTATCCCTAGCTCTGTTCTTCAGCATGTTGCTTCCCTTCAAATA AAAAAGGAATCTCAGAGTACAGACCAGAAAGATGAAACAAACTCTATCAAAAGAGAGGAGGAAACTAAGA AAGCTCTACCATCAAAAGATAAAGCTCTAGACTCTGAGGCTAATATAATGAAGCAAAACTCAGGAATTAT TGCCTCAGAAAATACCTCGAATAATTCATTGGATGATGGGGGTGATCTTTTGGATGAAGAAACCCTTAGG GAAGATGCCAGACCTTATGAATACTCTTATAGCACTGGGTCTCATACAGATGAAGACAAAGATGGTGATG AGGACTCTGGGAACAAGAATCAGAACAGTCCAAAAGAAAAACAAACTGTTCCAGAAGTTAGAGCTGGCTC TAAAAACATTGATATTATGGCACTCCAAAGCATCAGAAGTATACGGCCTCAAAAGTGTGTTAAGGTGAAG GTTGAACCGCAGGAGGGATCAGACAATCCAGAGAACCCAGATGACTTTCTAGTCCTTTCTAAGGACAGTA AATTTGAATTATCGGGGAACCAAGTTAAGGAACAGCAATCTAACTCACAGGCAGAGGCCAAGAAGGATTG TGAAGATTCTCTGGGGAAGGACAGTCTTAGAGAGAGATGGAGAAAACACCTAAAGGGCCCCTTAACTCAG AAGTACATTGGAATTTCACAGAACTTTAACAAAGAGGCAAACGTTCAGTTCTTTACAGAAATGAAGCCAT GTCAAGAAAATTCTGAACAGGATATCTCTGAATTACTTGGAAAAAGTGGAACTATTGAGAGTGGTGGAGT TCTAAAAACTGAGGATGGTTCTTGGAGTGGCATTTCTAGTTCTGCGGCCTTCTCTATTATCCCTAGGAGA GCTACAAAAGGAAGAAGAGGGAGCAGACATTTTCAGGGTCATTTACTGCTCCCAAGAGAACAGATGAAAC CAAAGCAACAGACAAAGGATGGGAGAAGCAGTGCTGCTGACTTCACAGTTTTGGATCTGGAAGATGAGGA TGAGGAAGATGAGAAAACTGATGATTCTCTTGATGAGATTGTGGATGTTGTCTCTGGCTACCAGAGTGAG GAGGTTGATGTAGAAAAGAATAACTATGTAGACTATCTTGAGGATGATGAGCAGGTGGATGTTGAAACAA TAGAAGAGCTTTCAGAGGAAATTAACTTTCCCTACAAGAAGACCACAGCAACTCACACACAGTCCTTCAA ACAGCAGTGTCATAGCCATATCTCTGCAGATGAAAAAGCTTCTGAAAAGAGTCGAAAGGTTTCATTAATT TCTTCAAAATTGAAAGATGATTGCTGGGGTGATAAACCACACAAAGAAACAGAAGCTTTTGCATATTACC GACGGACACACACTGCCAATGAGCGGCGTCGGCGTGGTGAAATGAGAGATCTTTTTGAAAAATTGAAGAT TACATTAGGATTACTTCATTCTTCCAAAGTTTCCAAAAGTCTCATTCTTAATCGGGCATTCAGTGAAATT CAAGGACTAACAGACCAGGCAGATAAATTGATAGGGCAGAAAAATCTCCTGAGTCGAAAACGAAGTATTT TAATACGGAAGGTATCGTCTCTTTCAGGGAAGACAGAAGAAGTGGTCCTGAAGAAGCTAGAGTATATTTA TGCAAAACAACAAGCACTAGAAGCACAAAAAAGAAAAAAGAAGCTGGGGTCAGATGAGTTTTGTGTGTCC CCCAGAATTGGCACACAGCTGGAGGGATCTTCTGCATCATCTGTAGATCTTGGACAGATGCTCATGAATA ACAGGAGGGGGAAACCTTTGATTCTTTCCAGAAAAAGAGACCAGGCTACAGAAAATGCCTCACCTTCTGA CACTCCGCACTCTTCTGCCAACCTGGTAATGACTCCACAAGGGCAGTTGCTTACCCTAAAAGGCCCCTTA TTCTCAGGACCAGTGGTAGCGGTTTCTCCTGCTCTCTTAGAAGGTGGTCTGAAGCCTCAAGTTGCAAGCA GTACTATGTCTCAGTCTGAGAATGATGACTTATTTATGATGCCACGAATTGTCAATGTGACATCATTGGC TGCAGAGGAAGATTTGGGAGGTATGAGTGGCAACAAATACCGTCACGAAGTTCCTGATGGCAAGCCACTT GACCACCTGAGAGACATTGCTGGGAGTGAAGCCAGCTCCTTAAAAGATACAGAGAGAATCTCTTCCAGAG GAAACCATCGAGATAGCAGAAAGGCACTGGGTCCAACACAGGTGCTTTTGGCAAATAAAGATTCTGGGTT TCCACATGTAGCTGATGTTTCCACTATGCAGGCAGCACAGGAATTTATACCTAAAAATATGTCTGGTGAT GTGAGAGGGCATCGGTATAAATGGAAGGAGTGTGAGTTGAGAGGAGAGAGACTAAAGTCAAAGGAGTCTC AGTTTCATAAATTAAAGATGAAAGATCTGAAGGACTCAAGCATAGAGATGGAACTGAGGAAAGTAGCCTC GGCTATCGAGGAAGCAGCCCTCCATCCCAGTGAGCTGCTGACTAACATGGAAGATGAGGATGACACTGAT GAGACTCTGACTTCACTGCTCAATGAAATTGCTTTTCTTAATCAACAGCTAAATGATGACTCTGGCCTAG CTGAACTGTCAGGTTCTATGGATACAGAATTCTCAGGAGATGCTCAGCGAGCTTTTATCAGTAAACTTGC TCCTGGGAACAGATCAGCTTTCCAAGTTGGACACTTGGGAGCAGGTGTGAAAGAGTTGCCTGATGTCCAA GAGGAGAGTGAATCCATCAGCCCCCTCCTCTTGCACTTGGAAGATGATGACTTTTCTGAGAATGAAAAAC AGCTTGGGGACACAGCCTCTGAGCCAGATGTCCTTAAGATTGTTATTGACCCTGAAATAAAGGATTCTCT TGTTTCCCATAGGAAATCTAGTGATGGAGGGCAGAGTACTTCTGGTCTCCCCGCAGAGCCTGAAAGTGTA TCTTCACCTCCCATCTTACACATGAAGACTGGCCCGGAGAACAGCAACACAGATACTTTGTGGAGGCCTA TGCCAAAGTTGGCCCCATTAGGTTTAAAAGTAGCTAATCCCCCCAGTGATGCCGATGGTCAGAGTCTCAA GGTGATGCCTGCCTTGGCACCTATAGCTGCCAAAGTTGGGTCAATTGGACACAAAATGAATTTAGCAGGA ATTGACCAGGAAGGCCGGGGGAGCAAGGTGATGCCTACATTGGCACCTGTTGTACCTAAATTGGGCAACT CTGGAGCTCCATCAAGTTCATCAGGGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001164274
Insert Size	8502 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001164274.1, NP_001157746.1
RefSeq Size	13304 bp
RefSeq ORF	8502 bp
Locus ID	29808
Gene Summary	Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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