Vwf (NM_011708) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6820430P06Rik; AI551257; B130011O06Rik; C630030D09; F8VWF; VWD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_011708, the custom clone sequence may differ by one or more nucleotides ATGAACCCTTTCAGGTATGAGATCTGCCTGCTTGTTCTGGCCCTCACCTGGCCAGGGACCCTCTGCACAG AAAAGCCCCGTGACAGGCCGTCGACGGCCCGATGCAGCCTCTTTGGGGACGACTTCATCAACACGTTTGA TGAGACCATGTACAGCTTTGCAGGGGGCTGCAGTTATCTCCTGGCTGGGGACTGCCAGAAACGTTCCTTC TCCATTCTCGGGAACTTCCAAGATGGCAAGAGAATGAGCCTGTCTGTGTATCTTGGGGAGTTTTTTGACA TCCATTTGTTTGCCAATGGCACCGTAACGCAGGGTGACCAAAGCATCTCCATGCCCTACGCCTCCCAAGG ACTCTACCTAGAACGCGAGGCTGGGTACTATAAGCTCTCCAGTGAGACCTTTGGCTTTGCGGCCAGAATC GATGGCAATGGCAACTTCCAAGTCCTGATGTCAGACAGACACTTCAACAAGACCTGTGGGCTGTGCGGTG ATTTTAACATCTTCGCGGAAGATGATTTTAGGACGCAGGAGGGGACCTTGACCTCAGACCCCTATGATTT TGCCAACTCCTGGGCCCTGAGCAGTGAGGAACAGCGGTGTAAACGGGCATCTCCTCCCAGCAGGAACTGC GAGAGCTCTTCTGGGGACATGCATCAGGCCATGTGGGAGCAATGCCAGCTACTGAAGACGGCATCGGTGT TTGCCCGCTGCCACCCTCTGGTGGATCCCGAGTCCTTTGTGGCTCTGTGTGAGAAGATTTTGTGTACGTG TGCTACGGGGCCAGAGTGCGCATGTCCTGTACTCCTTGAGTATGCCCGAACCTGCGCCCAGGAAGGGATG GTGCTGTACGGCTGGACTGACCACAGTGCCTGTCGTCCAGCTTGCCCAGCTGGCATGGAATATAAGGAGT GTGTGTCTCCTTGCCCCAGAACCTGCCAGAGCCTGTCTATCAATGAAGTGTGTCAGCAGCAATGTGTAGA CGGCTGTAGCTGCCCTGAGGGAGAGCTCTTGGATGAAGACCGATGTGTGCAGAGCTCCGACTGTCCTTGC GTGCACGCTGGGAAGCGGTACCCTCCTGGCACCTCCCTCTCTCAGGACTGCAACACTTGTATCTGCAGAA ACAGCCTATGGATCTGCAGCAATGAGGAATGCCCAGGGGAGTGTCTTGTCACAGGCCAATCGCACTTCAA GAGCTTCGACAACAGGTACTTCACCTTCAGTGGGATCTGCCAATATCTGCTGGCCCGGGACTGCGAGGAT CACACTTTCTCCATTGTCATAGAGACCATGCAGTGTGCCGATGACCCTGATGCTGTCTGCACCCGCTCGG TCAGTGTGCGGCTCTCTGCCCTGCACAACAGCCTGGTGAAACTGAAGCACGGGGGAGCAGTGGGCATCGA TGGTCAGGATGTCCAGCTCCCCTTCCTGCAAGGTGACCTCCGCATCCAGCACACAGTGATGGCTTCTGTA CGCCTCAGCTATGCGGAGGACCTGCAGATGGACTGGGATGGCCGTGGGCGGCTACTGGTTAAGCTGTCCC CAGTCTATTCTGGGAAGACCTGTGGCTTGTGTGGGAATTACAACGGCAACAAGGGAGACGACTTCCTCAC GCCGGCCGGCTTGGTGGAGCCCCTGGTGGTAGACTTCGGAAACGCCTGGAAGCTTCAAGGGGACTGTTCG GACCTGCGCAGGCAACACAGCGACCCCTGCAGCCTGAATCCACGCTTGACCAGGTTTGCAGAGGAGGCTT GTGCGCTCCTGACGTCCTCCAAGTTCGAGGCCTGCCACCACGCAGTCAGCCCTCTGCCCTATCTGCAGAA CTGCCGTTATGATGTTTGCTCCTGCTCCGACAGCCGGGATTGCCTGTGTAACGCAGTAGCTAACTATGCT GCCGAGTGTGCCCGAAAAGGCGTGCACATCGGGTGGCGGGAGCCTGGCTTCTGTGCTCTGGGCTGTCCAC AGGGCCAGGTGTACCTGCAGTGTGGGAATTCCTGCAACCTGACCTGCCGCTCCCTCTCCCTCCCGGATGA AGAATGCAGTGAAGTCTGTCTTGAAGGCTGCTACTGCCCACCAGGGCTCTACCAGGATGAAAGAGGGGAC TGTGTGCCCAAGGCCCAGTGCCCCTGCTACTACGATGGTGAGCTCTTCCAGCCTGCGGACATTTTCTCAG ACCACCATACCATGTGTTACTGTGAAGATGGCTTCATGCACTGTACCACAAGTGGCACCCTGGGGAGCCT GTTGCCTGACACTGTCCTCAGCAGTCCCCTGTCTCACCGTAGCAAAAGGAGCCTTTCCTGCCGGCCACCC ATGGTCAAGCTGGTGTGTCCTGCTGACAACCCACGGGCTCAAGGGCTGGAGTGTGCTAAGACGTGCCAGA ACTACGACCTGGAGTGTATGAGCCTGGGCTGTGTGTCTGGCTGCCTCTGTCCCCCAGGCATGGTCCGGCA CGAAAACAAGTGTGTGGCCTTGGAGCGGTGTCCCTGCTTCCATCAGGGTGCAGAGTACGCCCCGGGAGAC ACAGTGAAGATTGGCTGCAACACCTGTGTCTGCCGGGAGCGGAAGTGGAACTGCACGAACCATGTGTGTG ACGCCACTTGCTCTGCCATTGGTATGGCCCACTACCTCACCTTCGATGGACTCAAGTACCTGTTCCCGGG GGAGTGCCAGTATGTTCTGGTGCAGGATTACTGTGGCAGTAACCCTGGGACCTTTCAGATCCTGGTGGGA AATGAGGGTTGCAGCTATCCCTCGGTGAAGTGCAGGAAGCGGGTGACCATCCTGGTGGATGGAGGGGAGC TTGAACTGTTTGACGGAGAGGTGAACGTTAAGAGGCCCCTGAGAGATGAATCTCACTTTGAGGTGGTGGA GTCGGGCCGGTACGTCATCCTGCTGCTGGGTCAGGCCCTTTCTGTGGTCTGGGACCACCACCTCAGCATC TCTGTGGTCCTGAAGCACACATACCAGGAACAGGTGTGTGGCCTCTGCGGGAACTTTGATGGCATCCAGA ACAATGACTTCACCACTAGCAGCCTCCAGGTGGAGGAAGACCCCGTCAACTTTGGGAACTCCTGGAAAGT GAGCTCACAGTGTGCTGACACGAGAAAGCTGTCACTAGATGTTTCCCCTGCCACTTGCCACAACAACATC ATGAAACAGACGATGGTGGACTCAGCCTGCAGAATCCTTACCAGTGACGTCTTCCAGGGCTGCAACAGGC TGGTGGACCCTGAGCCATACCTGGACATCTGTATTTATGACACTTGCTCCTGTGAGTCCATCGGGGACTG CGCCTGTTTCTGTGACACCATTGCTGCCTATGCCCACGTGTGTGCCCAGCATGGCCAGGTGGTAGCCTGG AGGACACCCACACTGTGCCCCCAGAGCTGTGAAGAAAAGAATGTTCGGGAAAATGGCTATGAGTGTGAGT GGCGTTATAACAGCTGTGCGCCTGCTTGCCCAGTCACGTGTCAGCACCCTGAGCCTCTGGCTTGCCCTGT GCAGTGTGTGGAGGGTTGTCATGCACATTGCCCTCCAGGGAGAATCCTGGATGAACTTCTGCAGACCTGC GTAGACCCCCAAGACTGCCCCGTGTGTGAGGTGGCTGGTCGGCGCTTGGCTCCTGGAAAGAAAATCACCT TGAGTCCTGATGACCCTGCACACTGTCAGAATTGTCACTGTGATGGTGTGAACCTTACGTGTGAAGCCTG CCAAGAGCCCGGAGGCCTGGTGGCACCCCCAACTGATGCCCCAGTCAGCTCTACCACCCCATATGTTGAG GATACCCCCGAGCCCCCCCTGCACAACTTCTACTGCAGCAAGCTGCTGGATCTTGTCTTCCTGCTGGATG GCTCCTCTATGTTGTCCGAGGCTGAGTTTGAAGTGCTCAAAGCTTTTGTGGTGGGCATGATGGAGAGGTT ACACATCTCTCAGAAGCGCATCCGCGTGGCAGTGGTAGAGTACCATGATGGCTCCCGTGCCTACCTTGAG CTCAAGGCCCGGAAGCGACCCTCAGAGCTTCGGCGCATCACCAGCCAGATTAAGTATACAGGCAGCCAGG TGGCCTCTACCAGTGAGGTTTTGAAGTACACACTGTTCCAGATCTTTGGCAAAATTGACCGCCCTGAAGC CTCCCATATCACTCTGCTCCTGACTGCTAGCCAGGAGCCCCCACGGATGGCTAGGAATTTGGTCCGCTAT GTCCAAGGTCTGAAGAAGAAGAAGGTTATCGTGATCCCTGTGGGCATTGGGCCCCACGCCAGCCTCAAAC AGATCCGCCTCATCGAGAAGCAGGCCCCTGAAAACAAGGCTTTTCTGCTCAGTGGGGTGGATGAGCTGGA GCAGAGAAGAGATGAGATAGTCAGCTACCTCTGTGACCTTGCTCCCGAGGCCCCAGCCCCAACTCAGCCT CCACAGGTAGCCCACGTCACCGTGAGTCCAGGGATCGCTGGGATCTCGTCACCGGGACCAAAACGGAAGT CCATGGTTCTGGATGTGGTGTTTGTCCTGGAGGGGTCAGACGAAGTTGGTGAAGCCAACTTCAATAAGAG CAAGGAGTTCGTGGAGGAGGTAATCCAGCGCATGGACGTGAGCCCGGATGCAACGCGCATCTCAGTACTG CAGTATTCCTACACGGTAACCATGGAGTATGCCTTCAATGGGGCCCAGTCCAAGGAGGAGGTGCTGCGGC ACGTGCGAGAGATCCGCTACCAGGGCGGCAATAGGACAAACACTGGGCAGGCCCTGCAGTACCTTTCTGA GCACAGCTTCTCTCCCAGCCAAGGGGACCGGGTAGAGGCACCTAACCTGGTCTACATGGTCACGGGGAAC CCCGCCTCTGATGAGATCAAGAGGTTGCCTGGAGACATCCAGGTGGTACCCATTGGGGTGGGCCCCCATG CCAACATGCAGGAACTGGAGAGGATCAGCAGGCCCATCGCTCCCATCTTCATCCGGGACTTTGAGACACT TCCCCGAGAGGCTCCTGACCTGGTCCTGCAGACATGTTGCTCCAAGGAGGGTCTGCAACTGCCCACCCTC CCCCCTCTCCCTGACTGCAGCCAACCCCTGGATGTGGTCCTGCTCCTGGATGGCTCCTCTAGCTTGCCAG AGTCTTCCTTTGATAAAATGAAGAGTTTTGCCAAGGCTTTCATTTCAAAGGCCAACATTGGGCCCCACCT CACACAGGTGTCCGTGATACAGTATGGAAGCATCAATACCATTGATGTACCATGGAATGTGGTTCAGGAG AAAGCCCATCTACAGAGTTTGGTGGACCTCATGCAGCAGGAGGGTGGCCCCAGCCAGATTGGGGATGCTC TGGCCTTTGCCGTGCGCTATGTAACTTCACAAATCCACGGAGCCAGGCCTGGGGCCTCCAAAGCAGTGGT CATCATCATCATGGATACCTCCTTGGATCCCGTGGACACAGCAGCAGATGCTGCCAGATCCAACCGAGTG GCAGTGTTTCCCGTTGGGGTTGGGGATCGGTATGATGAAGCCCAGCTGAGGATCTTGGCAGGCCCTGGGG CCAGCTCCAATGTGGTAAAGCTCCAGCAAGTTGAAGACCTCTCCACCATGGCCACCCTGGGCAACTCCTT CTTCCACAAACTGTGTTCTGGGTTTTCTGGAGTTTGTGTGGATGAAGATGGGAATGAGAAGAGGCCTGGG GATGTCTGGACCTTGCCGGATCAGTGCCACACAGTGACTTGCTTGGCAAATGGCCAGACCTTGCTGCAGA GTCATCGTGTCAATTGTGACCATGGACCCCGGCCTTCATGTGCCAACAGCCAGTCTCCTGTTCGGGTGGA GGAGACGTGTGGCTGCCGCTGGACCTGCCCTTGTGTGTGCACGGGCAGTTCCACTCGGCACATCGTCACC TTCGATGGGCAGAATTTCAAGCTTACTGGTAGCTGCTCCTATGTCATCTTTCAAAACAAGGAGCAGGACC TGGAAGTGCTCCTCCACAATGGGGCCTGCAGCCCCGGGGCAAAACAAGCCTGCATGAAGTCCATTGAGAT TAAGCATGCTGGCGTCTCTGCTGAGCTGCACAGTAACATGGAGATGGCAGTGGATGGGAGACTGGTCCTT GCCCCGTACGTTGGTGAAAACATGGAAGTCAGCATCTACGGCGCTATCATGTATGAAGTCAGGTTTACCC ATCTTGGCCACATCCTCACATACACGCCACAAAACAACGAGTTCCAACTGCAGCTTAGCCCCAAGACCTT TGCTTCGAAGATGCATGGTCTTTGCGGAATCTGTGATGAAAACGGGGCCAATGACTTCACGTTGCGAGAT GGCACGGTCACCACAGACTGGAAAAGGCTTGTCCAGGAATGGACGGTGCAGCAGCCAGGGTACACATGCC AGGCTGTTCCCGAGGAGCAGTGTCCCGTCTCTGACAGCTCCCACTGCCAGGTCCTCCTCTCAGCGTCGTT TGCTGAATGCCACAAGGTCATCGCTCCAGCCACATTCCATACCATCTGCCAGCAAGACAGTTGCCACCAG GAGCGAGTGTGTGAGGTGATTGCTTCTTACGCCCATCTCTGTCGGACCAGTGGGGTCTGTGTTGATTGGA GGACAACTGATTTCTGTGCTATGTCATGCCCACCGTCCCTGGTGTATAACCACTGTGAGCGTGGCTGCCC TCGGCACTGCGATGGGAACACTAGCTTCTGTGGGGACCATCCCTCAGAAGGCTGCTTCTGTCCCCAACAC CAAGTTTTTCTGGAAGGCAGCTGTGTCCCCGAGGAGGCCTGCACTCAGTGTGTTGGCGAGGATGGAGTTC GACATCAGTTCCTGGAGACCTGGGTCCCAGACCATCAGCCCTGTCAGATCTGTATGTGCCTCAGTGGGAG AAAGATTAACTGCACTGCCCAGCCGTGTCCCACAGCCCGAGCTCCCACGTGTGGCCCATGTGAAGTGGCT CGCCTCAAGCAGAGCACAAACCTGTGCTGCCCAGAGTATGAGTGTGTGTGTGACCTGTTCAACTGCAACT TGCCTCCAGTGCCTCCGTGTGAAGGAGGGCTCCAGCCAACCCTGACCAACCCTGGAGAATGCAGACCCAC CTTTACCTGTGCCTGCAGGAAAGAAGAGTGCAAAAGAGTGTCCCCACCCTCCTGCCCCCCTCACCGGACA CCCACTCTCCGGAAGACCCAGTGCTGTGATGAATACGAGTGTGCTTGCAGCTGTGTCAACTCCACGCTGA GCTGCCCACTTGGCTACCTGGCCTCAGCCACTACCAATGACTGTGGCTGCACCACGACCACCTGTCTCCC TGACAAGGTTTGTGTCCACCGAGGCACCGTCTACCCTGTGGGCCAGTTCTGGGAGGAGGGCTGTGACACG TGCACCTGTACGGACATGGAGGATACTGTCGTGGGCCTGCGTGTGGTCCAGTGCTCTCAAAGGCCCTGTG AAGACAGCTGTCAGCCAGGTTTTTCTTATGTTCTCCACGAAGGCGAGTGCTGTGGAAGGTGCCTGCCCTC TGCTTGCAAGGTGGTGGCTGGCTCACTGCGGGGCGATTCCCACTCTTCCTGGAAAAGTGTTGGATCTCGG TGGGCTGTTCCTGAGAACCCCTGCCTCGTCAACGAGTGTGTCCGCGTGGAGGATGCAGTGTTTGTGCAGC AGAGGAACATCTCCTGCCCACAGCTGGCTGTCCCTACCTGTCCCACAGGCTTCCAACTGAACTGTGAGAC CTCAGAGTGCTGTCCTAGCTGCCACTGTGAGCCTGTGGAGGCCTGCCTGCTCAATGGCACCATCATTGGG CCCGGGAAGAGTGTGATGGTTGACCTATGCACGACCTGCCGCTGCATCGTGCAGACAGACGCCATCTCCA GATTCAAGCTGGAGTGCAGGAAGACTACCTGTGAGGCCTGCCCCATGGGCTATCGGGAAGAGAAGAGCCA GGGTGAATGCTGTGGGAGATGCTTGCCTACAGCTTGCACTATTCAGCTAAGAGGAGGACGGATCATGACC CTGAAGCAAGATGAGACATTCCAGGATGGCTGTGACAGTCATTTGTGCAGGGTCAACGAGAGAGGAGAGT ACATCTGGGAGAAGAGGGTCACGGGCTGCCCACCATTTGATGAACACAAGTGTCTGGCTGAAGGAGGCAA AATCGTGAAAATTCCAGGCACCTGCTGTGACACATGTGAGGAGCCTGATTGCAAAGACATCACAGCCAAG GTGCAGTACATCAAAGTGGGAGATTGTAAGTCCCAAGAGGAAGTGGACATTCATTACTGCCAGGGAAAGT GTGCCAGCAAAGCTGTGTACTCCATTGACATCGAGGATGTGCAGGAGCAATGCTCCTGCTGCCTGCCCTC GAGGACGGAGCCCATGCGCGTGCCCTTGCACTGCACCAATGGCTCTGTCGTGTACCACGAGGTCATCAAC GCCATGCAGTGCAGGTGTTCTCCCCGGAACTGCAGCAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_011708
Insert Size	8442 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011708.3, NP_035838.3
RefSeq Size	8817 bp
RefSeq ORF	8442 bp
Locus ID	22371
UniProt ID	Q8CIZ8
Gene Summary	Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.[UniProtKB/Swiss-Prot Function]

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