Flnc (NM_001081185) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110055E19Rik; ABP-280; ABPL; Fln2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225397 representing NM_001081185 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACAACAGCAACTATTCGGACGCCTCCGGCCTCGGCCTGGTTGACGAGGCGGACGAGATGCCGT CCACGGAGAAGGACCTAGCGGAGGACGCACCGTGGAAGAAGATCCAGCAGAACACATTCACGCGGTGGTG CAACGAGCATCTCAAGTGTGTGGGCAAGCGCCTGACCGACCTGCAGCGAGACCTCAGCGACGGGCTGCGC CTCATCGCGCTGCTCGAAGTGCTCAGCCAGAAGCGCATGTACCGCAAGTTCCACCCGCGCCCCAACTTCC GCCAAATGAAGCTGGAAAATGTGTCTGTGGCCCTGGAGTTCCTGGAGCGGGAACACATCAAGCTCGTGTC CATCGACAGCAAGGCTATTGTGGATGGAAACCTGAAGCTGATCCTAGGCCTGATCTGGACGCTGATCCTT CACTACTCCATTTCCATGCCCATGTGGGAGGATGAAGATGATGAGGATGCCCGCAAACAGACACCCAAGC AGCGACTGCTTGGCTGGATCCAGAACAAAGTGCCCCAGCTGCCCATCACCAACTTTAACCGAGACTGGCA GGACGGCAAGGCTCTGGGTGCCCTGGTTGACAACTGTGCCCCTGGTCTCTGCCCTGATTGGGAAGCCTGG GACCCCAACCAGCCCGTGCAGAATGCCAGAGAGGCCATGCAGCAAGCTGATGACTGGCTCGGGGTGCCCC AGGTGATTGCTCCTGAAGAAATCGTGGATCCCAATGTGGATGAACACTCTGTCATGACCTACCTGTCCCA GTTTCCCAAGGCCAAGCTCAAACCCGGTGCCCCTGTTCGCTCTAAGCAGCTGAATCCTAAGAAAGCCATT GCCTATGGGCCTGGCATTGAGCCCCAGGGGAACACTGTGCTGCAGCCTGCCCACTTCACCGTGCAGACAG TAGATGCTGGTGTAGGGGAGGTGCTGGTCTACATTGAGGACCCCGAGGGACACACGGAGGAGGCCAAAGT GGTTCCCAACAATGACAAAGACCGCACCTATGCCGTCTCCTATGTGCCCAAGGTTGCTGGGTTACACAAG GTGACCGTGCTCTTTGCTGGCCAGAACATCGAGCGTAGTCCCTTTGAGGTGAATGTGGGCATGGCTCTCG GGGATGCCAACAAGGTATCAGCCCGTGGCCCTGGTCTGGAACCTGTGGGTAACGTGGCCAACAAACCCAC CTACTTTGACATCTATACTGCCGGGGCTGGCACTGGTGATGTTGCTGTGGTGATCGTGGACCCACAGGGA CGTCGGGATACAGTCGAGGTGGCCCTGGAGGACAAGGGTGACAACACGTTCCGCTGCACGTACAGGCCCG TGATGGAGGGGCCACACACCGTGCATGTGGCCTTTGCTGGTGCACCCATCACCCGGAGTCCTTTCCCTGT CCATGTGGCAGAAGCCTGTAACCCCAACGCCTGCAGAGCCTCTGGGCGGGGCCTGCAGCCCAAAGGTGTG CGTGTGAAAGAGGTGGCTGACTTCAAGGTGTTCACCAAGGGTGCTGGCAGCGGGGAACTCAAGGTCACCG TCAAAGGTCCAAAGGGCACAGAGGAGCCAGTGAAAGTTCGGGAGGCTGGAGATGGGGTGTTCGAGTGCGA GTACTACCCAGTGGTACCTGGGAAGTATGTGGTGACCATCACCTGGGGTGGCTATGCCATTCCCCGCAGT CCTTTTGAGGTTCAGGTGAGCCCAGAGGCCGGAGCCCAGAAAGTGCGGGCATGGGGTCCTGGGTTGGAGA CTGGCCAGGTGGGCAAGTCGGCTGACTTCGTGGTGGAAGCCATCGGTACAGAAGTGGGAACGCTGGGCTT CTCCATCGAGGGGCCCTCACAAGCCAAGATTGAATGTGATGACAAAGGTGACGGCTCCTGTGACGTGCGG TACTGGCCCACGGAGCCTGGGGAGTATGCTGTGCATGTCATCTGCGATGATGAAGACATCCGGGACTCGC CTTTCATCGCCCACATCCAGCCAGCCCCACCAGACTGCTTCCCAGACAAGGTGAAAGCATTTGGACCTGG CCTGGAGCCCACTGGCTGCATAGTGGACAGGCCTGCCGAGTTCACCATTGATGCCCGTGCTGCTGGCAAG GGAGATTTGAAGCTCTATGCCCAGGATGCAGATGGCTGCCCCATCGACATCAAGGTGATCCCCAACGGTG ACGGTACCTTCCGCTGCTCCTACGTGCCTACCAAACCCATCAAGCACACTATCATCGTCTCCTGGGGAGG TGTCAACGTGCCTAAGAGCCCCTTCAGGGTGAATGTGGGAGAAGGCAGCCATCCTGAGCGGGTGAAGGTA TATGGCCCTGGAGTAGAGAAGACAGGCCTTAAAGCCAATGAACCCACCTATTTCACAGTGGACTGCAGTG AAGCCGGGCAAGGTGATGTGAGTATTGGCATCAAGTGTGCTCCAGGCGTGGTGGGGCCTGTGGAAGCTGA CATCGACTTTGACATCATCAAGAATGACAACGATACCTTCACAGTCAAGTACACACCCCCCGGGGCCGGC CACTACACGATCATGGTGTTGTTTGCCAACCAGGAGATCCCTGCCAGTCCCTTCCACATCAAGGTGGACC CATCCCATGACGCTAGCAAGGTCAAGGCTGAGGGTCCTGGGCTGAGCCGCACAGGTGTGGAAGTCGGAAA GCCGACTCACTTTACCGTGCTGACCAAGGGAGCTGGCAAGGCTAAGCTGGACGTGCACTTTGCTGGGGCA GCCAAGGGCGAGGCTGTGCGGGACTTTGAAATCATTGACAACCATGACTATTCATACACTGTCAAGTACA CAGCTGTGCAGCAGGGCAACATGGCAGTAACAGTGACCTACGGTGGGGACCCTGTCCCCAAAAGTCCCTT TGTGGTGAATGTGGCACCCCCACTGGACCTCAGCAAAGTCAAAGTTCAAGGCCTCAACAGCAAGGTAGCT GTGGGACAGGAACAGGCGTTCTCGGTGAACACCAGAGGGGCTGGTGGTCAGGGACAACTCGATGTGCGGA TGACCTCACCCTCCCGACGGCCTATCCCCTGCAAGCTGGAGCCTGGGGGTGGAGCAGAAGCCCAGGCTGT CCGCTACATGCCCCCTGAAGAGGGACCTTACAAGGTGGACATCACCTACGATGGTCACCCAGTGCCTGGC AGCCCCTTTGCTGTGGAAGGTGTCCTGCCCCCTGACCCCTCTAAGGTCTGCGCGTATGGCCCTGGTCTCA AGGGCGGGCTTGTAGGCACCCCGGCACCATTCTCCATTGACACCAAGGGGGCCGGCACAGGAGGCCTGGG GCTGACTGTCGAGGGCCCCTGCGAAGCCAAGATCGAGTGCCAAGACAATGGTGACGGATCTTGTGCAGTC AGCTACCTGCCCACGGAGCCAGGCGAGTACACCATCAATATCCTGTTTGCTGAAGCCCACATCCCTGGCT CGCCCTTCAAGGCCACCATCCAGCCTGTGTTCGACCCAAGCAAGGTGAGGGCCAGTGGGCCAGGCCTGGA GCGTGGCAAGGCTGGAGAGGCAGCCACCTTCACCGTGGACTGCTCAGAAGCGGGTGAGGCCGAGCTGACC ATCGAGATCCTGTCGGACGCTGGTGTCAAAGCGGAGGTGCTGATCCAAAACAACGCGGACGGCACCTACC ACATCACCTACAGCCCTGCCTTCCCCGGCACCTACACCATCACCATCAAGTATGGCGGCCACCCCATACC CAAGTTCCCCACCCGTGTCCACGTGCAGCCTGCAGTTGATACCAGTGGCATCAAGGTCTCTGGGCCTGGT GTGGAGCCACATGGTGTCCTGCGGGAGGTGACCACCGAGTTTACTGTGGATGCGAGATCTCTAACAGCTA CAGGCGGCAACCATGTGACAGCTCGTGTCCTCAACCCCTCCGGTGCTAAGACAGATACCTATGTGACAGA CAACGGGGATGGCACCTACCGGGTGCAGTACACTGCCTATGAAGAGGGTGTGCATTTGGTAGAGGTGCTG TATGACGAAGTAGCTGTGCCCAAGAGCCCCTTCCGAGTGGGTGTGACTGAGGGCTGTGACCCCACCCGAG TCCGGGCCTTCGGGCCAGGTCTGGAAGGTGGCTTGGTGAACAAGGCCAACCGCTTCACTGTGGAAACCAG GGGTGCTGGCACAGGAGGCCTTGGCCTAGCCATTGAGGGCCCCTCAGAAGCTAAGATGTCCTGCAAGGAC AACAAGGACGGAAGCTGCACCGTGGAGTACATCCCCTTTACCCCTGGAGACTATGATGTCAACATCACCT TTGGGGGACAGCCCATTCCAGGAAGCCCATTCCGAGTTCCTGTGAAGGATGTGGTAGACCCTGGGAAGGT GAAGTGCTCAGGGCCAGGGCTAGGGACCGGCGTCAGGGCTCGAGTACCTCAGACCTTCACAGTGGACTGC AGTCAAGCTGGCCGGGCCCCTCTGCAGGTGGCCGTGCTGGGCCCCACAGGTGTGGCTGAGCCTGTGGAGG TGCGTGACAATGGCGATGGTACCCACACTGTCCACTACACACCTGCCACTGATGGTCCCTATACTGTAGC TGTCAAGTATGCTGACCAGGAGGTGCCACGAAGCCCTTTCAAGATCAAAGTGCTTCCATCCCATGATGCC AGCAAAGTGCGGGCCAGTGGCCCTGGCCTCAATGCCTCGGGCATCCCTGCCAGCCTGCCCGTGGAGTTCA CCATTGATGCTCGTGATGCTGGGCAGGGGCTTCTTACCGTGCAGATCCTGGATCCCGAGGGTAAACCCAA GAAGGCCAACATCCGAGACAATGGGGATGGCACATATACTGTGTCCTACCTGCCCGACATGAGTGGTCGG TACACCATCACCATCAAGTACGGCGGCGATGAGATCCCTTACTCGCCCTTCCGCATCCATGCCCTGCCTA CAGGGGATGCCAGCAAGTGCCTTGTTACAGTGTCCATTGGAGGCCATGGACTGGGTGCCTGCCTGGGTCC CAGAATCCAGATCGGAGAGGAGACTGTGATCACAGTGGACGCCAAGGCAGCAGGCAAGGGGAAGGTGACC TGCACAGTATCCACACCCGATGGGGCAGAGCTCGATGTGGACGTGGTCGAGAACCATGATGGCACCTTCG ACATCTACTACACAGCTCCCGAGCCGGGCAAATATGTCATCACCATCCGCTTTGGGGGCGAACACATCCC CAACAGCCCTTTCCACGTGCTGGCCTGTGACCCGCTGCCTCACGTGGAGGAGCCCGCGGAAATGCTGCAG ATGCGCCAGCCCTATGCCCCTCTTCGGCCTGGCACCTGCCCCACGCACTGGGCCACAGAGGAGCCCGTGG TGCCTGTGGAGCCTCTGGAATCCATGCTGAGGCCTTTCAACTTGGTCATCCCCTTCACTGTGCAGAAAGG GGAGCTGACAGGGGAAGTCCGGATGCCTTCTGGAAAAACAGCGCGCCCCAACATCACTGACAACAAGGAT GGAACCATCACAGTGAGATACGCACCGACAGAGAAGGGCCTGCACCAGATGGGGATCAAGTACGACGGCA ACCATATCCCTGGGAGCCCTCTGCAGTTCTATGTGGATGCCATCAACAGCCGCCATGTCAGTGCCTACGG GCCAGGCCTGAGCCATGGCATGGTCAACAAGCCTGCCACCTTCACAATTGTCACCAAGGATGCTGGGGAA GGGGGGCTGTCACTGGCTGTGGAGGGCCCATCCAAGGCGGAGATCACCTGCAAGGACAACAAGGATGGCA CCTGTACAGTGTCCTACCTGCCCACAGCACCCGGAGACTACAGCATCATCGTGCGCTTTGACGACAAGCA CATCCCAGGGAGCCCCTTCACGGCCAAGATCACAGGTGACGACTCAATGAGAACATCACAGCTTAATGTG GGCACCTCCACCGATGTGTCACTGAAGATCACGGAGGGTGACCTCAGCCAGCTGACAGCCAGCATCCGTG CTCCTTCAGGCAATGAAGAGCCATGCCTGCTGAAGCGCCTGCCCAACAGGCACATTGGGATCTCCTTTAC CCCCAAGGAGGTCGGTGAGCACGTGGTGAGCGTGCGCAAGAGTGGCAAGCACGTCACCAACAGCCCCTTT AAGATTTTGGTGGGGCCTTCGGAGATCGGGGATGCCAGCAAAGTTCGAGTCTGGGGCAAAGGCCTTTCCG AGGGACAAACCTTCCAAGTGGCAGAGTTCATTGTGGACACTCGCAATGCAGGTTATGGAGGCCTGGGGCT GAGCATCGAAGGCCCTAGCAAGGTGGACATTAACTGTGAGGATATGGAAGACGGTACCTGCAAAGTCACC TACTGCCCGACTGAACCCGGCACCTACATCATCAATATTAAGTTTGCTGACAAGCACGTACCTGGAAGCC CCTTCACCGTGAAGGTGACTGGCGAGGGACGCATGAAGGAAAGCATCACTCGGCGCAGACAGGCACCCTC CATTGCCACCATTGGTAGCACCTGTGACCTCAACCTCAAGATCCCAGGAAACTGGTTTCAGATGGTGTCT GCCCAGGAGCGCCTGACTCGCACCTTCACGCGGAGCAGTCATACGTACACCCGCACAGAGCGGACAGAGA TCAGCAAGACTCGGGGTGGGGAGACCAAGCGTGAAGTCCGAGTGGAGGAGTCCACCCAGGTCGGCGGAGA CCCTTTCCCTGCTGTTTTCGGCGATTTTCTAGGCCGGGAACGCCTGGGCTCCTTCGGCAGCATCACCCGG CAGCAGGAAGGTGAGGCCAGCTCTCAGGACATGACAGCCCAGGTGACAAGTCCGTCTGGCAAGACAGAAG CCGCAGAGATCGTTGAGGGAGAAGACAGCGCATACAGTGTGCGATTCGTGCCCCAGGAGATGGGTCCCCA CACAGTCACTGTCAAGTACCGCGGCCAACACGTGCCCGGAAGCCCTTTTCAGTTCACTGTGGGTCCGCTA GGTGAAGGTGGTGCCCACAAGGTGCGAGCTGGAGGCACAGGGCTGGAGCGAGGTGTAGCTGGTGTGCCAG CTGAGTTTAGCATCTGGACCCGAGAAGCTGGTGCTGGGGGATTGTCCATCGCTGTGGAAGGTCCCAGCAA GGCAGAGATTGCATTTGAGGACCGCAAAGATGGTTCTTGTGGCGTCTCCTATGTTGTCCAGGAGCCAGGT GACTACGAGGTCTCCATCAAGTTCAATGATGAGCACATCCCAGACAGCCCGTTCGTGGTGCCCGTGGCCT CCCTCTCAGACGACGCTCGCCGCCTCACTGTTACCAGCCTCCAGGAGACAGGGCTTAAAGTGAACCAGCC AGCATCTTTCGCGGTGCAGCTTAATGGGGCACGTGGTGTGATTGATGCCCGGGTACACACACCCTCAGGA GCTGTGGAGGAGTGCTATGTCTCTGAACTGGACAGTGATAAGCATACCATCCGCTTCATCCCCCATGAGA ACGGTGTCCACTCAATTGATGTCAAGTTCAACGGCGCCCACATCCCTGGAAGTCCCTTCAAGATCCGTGT TGGGGAGCAGAGCCAGGCTGGGGACCCAGGCTTGGTGTCAGCTTATGGGCCTGGGCTTGAAGGAGGCACT ACGGGCGTATCATCAGAGTTTATTGTGAACACCCAGAATGCAGGCTCAGGGGCTTTGTCTGTCACCATTG ATGGGCCTTCCAAGGTGCAGCTGGACTGTCGGGAATGTCCCGAGGGCCATGTAGTCACTTACACTCCCAT GGCTCCTGGTAACTACCTCATTGCTATCAAGTATGGTGGCCCTCAGCACATTGTGGGCAGTCCCTTCAAA GCAAAGGTCACAGGTCCCCGGTTGTCTGGAGGCCACAGCCTTCACGAAACATCCACAGTTCTGGTGGAGA CGGTGACCAAGTCCTCCTCGAGCCGCGGTGCCAGTTACAGTTCCATCCCCAAGTTCTCCTCGGATGCCAG CAAGGTGGTAACAAGGGGCCCTGGTCTGTCCCAGGCCTTTGTGGGTCAGAAGAACTCATTCACCGTGGAC TGCAGCAAAGCAGGCACCAACATGATGATGGTGGGCGTGCATGGGCCCAAGACCCCCTGCGAAGAGGTAT ATGTGAAGCACATGGGAAACCGGGTCTACAACGTCACCTACACTGTCAAGGAGAAGGGAGACTACATCCT CATTGTCAAGTGGGGTGATGAAAGTGTCCCTGGGAGCCCCTTCAAGGTCAATGTGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081185
Insert Size	8181 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081185.1, NP_001074654.1
RefSeq Size	9166 bp
RefSeq ORF	8181 bp
Locus ID	68794
UniProt ID	Q8VHX6
Gene Summary	Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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