Abca12 (NM_175210) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4832428G11Rik; 4833417A11Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225368 representing NM_175210 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCCAGTTTCATCAGCTTCGGATCCTGGTGTGGAAAAATTGGCTAGGTGTCAAAAGGCAGCCGC TTTGGACACTTGTCTTGATCTTATGGCCAGTCATTATTTTCATAATTCTGGCTATTACCCGGACAAAATT CCCGCCAACAGCAAAACCAACTTGTTACCTCGCACCTCGAAACCTTCCCAGTGCTGGATTCTTCCCATTC CTACAAACCCTGCTCTGTGACACAGACTCTAAATGTAAAGACACGCCCTATGGACCACGAGATCTGCTCC GCAGGAAGGGGATTGATGGACCGCTATTTAAAGAAAGCGAAATTCTGAAAAAGCCGTCCAACCCGAAGAG GGACAGCAATTTATCACTCCGGAGCACCCAAGTTCCAGAAAGAAGTCACACATCATTGGCCACAGTACCT CCAAGACCAAGCTATGATTTGGAAGGAACAGGCACAGAAAATTTCAATGGTAGCCAATTGCTTACACGCA TCCTTGGTTTGGAAAAGCTGCTGAAGCAAAATTCAACTCCAGAAGACATACGAAGAGAGCTGTGTGAGAG CTACCCTGGGTACACTGCGGACTATGCCTTCTCTTGGGTCACCCTAGGAAAAAACGTTTTTAACAAATTT TGCCTTTCGAACATGACACTTTTAGAGTCTTCACTCCAGGAACTAAAATATCAAGTCTCTCAGATGTCAA GTGACCCCGACAATCAGAAGAGGGTGTTCCGGGGACTTGTCCAGGTGCTGTCCTTTTTCTCTCAAGTGCA GCAGCAAAGAGAAGTGTGGCAACTTCTGTCGAGCTTACCAGATGTGTTTCAGAATGGCACCTCATTGAGC AGTCTATTTGGTGTTCTTCAAAAGGCAAACAGGGTGCTGCTGGTAGTGCAGAAGGTTTACCCACGTGTTC AAACTGATGAAGGATTCAGCACCCTCCAGAAGTCTGTTAAACATCTGCTGAACACTCTGGACTCCCCAAT GCAAGGTGACAATTCAACGCACGCATGGAGTGATGATGATGAGCAGACACTGTCCCCGAGCAGTCTGGCT GCACAGCTCCTGATCCTGGAAAACTTTGAAGATGCCATCTTGAACATATCATCAAATAGTCCATATTCTC CTTATTTGGCGTGTGTAAGGAACATGACTGACAACTTGGCTAAGGGATCACCAGACAATCTAAAACTTCT GCAGTCCACAATTCACTTCAGAAAATCTTTCCTTCAAAATGGCTCCTCCGAGGACAGCTTCCCTCCATTT CTTGAGATCCTGAAATCCAAGCTGTCTCAACTTCGAAATTTGACGGAACTTCTCTGCGAATCAGAGACTT TCAGTTCGATCAAGAAGTCTTGCCAGTTCTCTAACATGAGCTTCGAGAGGCTGTGTGAAGACCATGCCTT TCACGTGCAACTGATTGAAGCGGCAGAGCTGGGCACTGACCTCACCACTGGCTTACTGTACCATGACAAT ATAATATCTGCAAAGCTGAGGGGTTTGCTCACTGGAGATCCCAGCAAAATTAATCTCAATGTGGACTGGC TCCTGGAACAGGCATTGCAAATGAATTACTTGGAGAATATCACACGGCTCATACCAACCGTAGAGGCCAT GCTGCATGTCAACACCAGTGCAGACGCTTCTGAGAAGCCAGGTCAATTAAGAGAAATGTTTAAAAACATA GATTTGCTGAAAGAAGATCTGAGGGCGATAGGGATGTCCAACACAAGTATCGACAAGCTGTTGGCCATTC CCATTCCAGACAATAGAGCCGAGATAATTTCTCGTGTATTCTGGTTGCACTCATGTGATACCAACGTCAC CAACCCCAAACTGGAAGATGCCATGAAGGAGTTCTGCAAGCTGCCTCTTCCAGAGAGATCACATCAGTCC TACCTCATCGGACTCACCCTTCTGCACTACTTAGACATTTACAATTTCACATACAAGGTGTTTTTCCCAA GGAAGGATCAAAAGCCAATGGAAAGGATGATGGAGCTTTTCATAAAGCTGAGAGAGATTCTCAATCAGTT GGCTTCCGGCACACATCCACTGCTGGACAAAATGAGATCCCTGAGACAAATGCACCTGCCCAGGAGTGTT CCATTAACACAGGCCATGTACAGAAACACTCGAATGAACTCGCCAGCAGGATCGTTCAGTACTATCTCCC AGGCTCTGTGCTCCCAGGGGATCACCACCGAGTACTTAACTGCTATGCTGCCTTCTTCCCAGAAGCCAAA AGGCAACCACACCAAGGATTTTCTCACTTATAAATTAACTAAAGAAGAAATTGCTTCAAAATACGGGATT CCTTTAAATGCTACACCATTTTGCTTCTCCCTTTACAAAGACATCATTAACATGCCTGCTGGGCCTGTGA TTTGGGCCTTCTTGAAACCCATGTTGTTGGGAAAGATTTTATATTCACCATATAACCCAACTACAAAGGC CATTATGGAAAAGTCCAATGTAACTCTAAGACAGCTGGCGGAATTAAGAGAAAAATCTCAGGAGTGGATG GATAAGTCACCAATTTTCATGAATTCCTTCCACCTGCTAAACCAGACGATCCCAATGCTCCAGAATACTC TACGGAACCCCTTTGTGCAAGTTTTTGTGAAGTTCTCCGTGGGACTCGATGCTGTCGAACTCCTGAAGCA GATAGACGACCTTGATGTTCTCAGGCTGAAATTAGTGAACAACATTGACATCATTGATCAGCTCAACACA CTGTCTTCCCTGACTGTAAATATTTCCTCCTGTGTTCTTTACGACCGTATTCAGGCGTCAGACACTGTAG AGGAGATGGAGACGGTGGCCGAACAACTCTACAAGAGCAACGAGCTCTTTGGAAGTGTCATTTTTAAGCT TCCATCTAACGGAAGTTTGCACAGAGGGTTTGACCCTGAAAAAGTGTCTCTTCCTCCTATCGTAAGATAT ACCATCCGCATGAGTCTGAAGACGGCACAGACCACAAGGAGCATAAGAACAAAGATCTGGGCCCCAGGGC CACACAACTCTCCATCCCATAACCAGATCTATGGCCGGGCTTTCATTTATCTGCAGGATAGCATTGAGAG AGCAATTATTGAGTTACAAACAGGAAGGAACTCACAGGAAGTCGCCGTCCAGGTTCAAGCGGTTCCTTAT CCCTGCTTCATGAAGGACAATTTCCTCACCAGTGTCTCCTATTCCCTCCCGATTGTGCTTATGGTCGCCT GGGTTGTATTTATAGCTGCCTTTGTTAAAAAGCTAGTCTACGAGAAGGACCTTCGGCTCCATGAGTACAT GAAGATGATGGGTGTCAACTCCTGCAGCCATTTCTTTGCCTGGCTTATAGAGAGTATTGGGTTCTTGCTG GTCACCATTGCAATCCTCATCGTTATCCTCAAGTTTGGCAATATTCTTCCCAAGACAAACGGCTTCATTT TGTTCCTGTACTTCTCAGACTACAGCTTCTCAGTGATTGCCATGAGCTATCTCATCAGTGTCTTCTTCAA CAACACCAACATTGCAGCTCTGATTGGAAGCCTCATCTATGTCATCGCCTTTTTCCCATTTATTGTTCTG GTTACAGTTGAGGATGAGCTGAGCTACGTTATTAAAGTATTCATGAGCCTGCTGTCCCCGACAGCATTCA GCTATGCAAGCCAATATATTGCACGGTATGAAGAACAAGGCGTTGGTCTTCAGTGGGAAAATATGTACAA ATCCCCTGTTCAGGATGACACCACCTCATTTGGCTGGCTGTGCTGTCTAATCCTAGCAGACTCCTTCATT TATTTCTTTATTGCTTGGTACGTCAGGAATGTTTTCCCAGGGACATATGGAATGGCAGCTCCATGGTATT TTCCCATCCTTCCTTCCTATTGGAAAGAGCGCTTTGGATGCGCAGAGGTGAAGCATGAGAAAAGCAATGG CTTAATGTTTACTAATATCATGATGCAGAACACCAACCCTTCTGCCAGTAAGACAAGTCCTGACTGTGCA TTTCCCTCCAACATTGAACCTGAACCTAAAGATCTCCAAGTTGGAGTTGCCCTTCATGGGGTCACAAAGA TCTATGGCTCAAAAACAGCTGTTGAGAACCTCAATCTGAACTTCTACGAAGGGCACATTACATCACTGTT GGGGCCAAACGGAGCTGGCAAAACCACAACAATCTCCATGCTGACTGGGCTGTTTGGTGCCACAGCGGGT ACCATTTTTGTATACGGAAAAGATATCAAGACAGACCTGAATACTGTTCGGAAAAACATGGGGGTCTGCA TGCAACATGACGTCTTGTTCAGTTACCTAACCACCAAGGAACACCTTCTCCTGTATGGCTCCATCAAAGT TCCTCACTGGACCAAAACACAGCTCCATGAGGAGGTTAAAAGGACTTTAAAAGACACTGGACTGTATAGT CATCGCCATAAGAGAGTTGGAACGTTGTCGGGAGGAATGAAGAGGAAGTTGTCCATATCCATAGCTCTCA TCGGTGGCTCCAGGGTAGTGATTTTGGATGAACCATCCACGGGCGTTGACCCATGTTCTCGCCGAAGCAT ATGGGATGTCATATCCAAGAACAAAACTGCAAGAACAATCATTCTGTCCACACACCACTTGGATGAGGCC GAAGTGCTGAGTGACCGCATCGCCTTCCTGGAACAGGGTGGGCTCCGGTGCTGTGGATCTCCATTTTACC TCAAGGAAGCTTTTGGGGATGGTTACCACCTCACACTCACCAAGAAGAAGAGTCCAAACCTAGACACAAA TGCAATCTGTGACACAGTGGCAGTGACAGCAATGATCCAGTCGCACCTTCCTGAAGCCTACCTCAAAGAG GATATTGGGGGAGAGCTTGTATATGTGCTCCCTCCATTCAGCACGAAAGTCTCAGGAGCTTACCTGTCCC TCCTGAGAGCACTGGACAAGGGCATGGGTAAACTCAACATCGGCTGCTATGGCATTTCAGACACCACTGT TGAAGAGGTCTTCCTGAACTTGACAAAAGATTCGCAAAAAAGTAGTAATATGAGTCTGGAGCACTTAACA CAGAGGAAAGTTGGGAACCCCAGTGCCAACGGGACCTCCACTCCTGACGACCTGTCTGTGAGCAGCAGCA ACTTCACCGACAGAGACGACAAAGTCCTGACAAGAAGCGAGAAGCTGGAGGGCTTTGGACTCTTACTGAA GAAGATAATGGCTATTCTCATCAAGAGGTTCCACCACACCCGCCGGAACTGGAAAGGTCTCATTGCTCAG GTCATTCTCCCCATCGTCTTTGTAGCCACGGCCATGGGTCTCGGCACACTGAGAGATTCCAGCAACAGTT ACCCAGAGATCATGATCTCTCCGTCCATTTACGGCACCTCCGAACAGACAGCCTTCTACGCGAATTTTGA TCCAAGCACCAGTGGTCTGGTCTCAGCTCTCTGGAATTTCCCTGGCATTGACAATGTGTGTTTGAATACT AGTGACTTGCAGTGTTTAAAAAAAGATGATCTGGGAAAGTGGAACACAAGTGGAGAAGCTATCGACAACT TTGGTGTTTGCTCCTGTTCCGACAATGTCCAGGAATGCCCCAAATTCAACTATCATCCACCTCATAGAAG AACTTACTCTTCCCAAGTTATTTATAACCTCACTGGAAAACACATGGAAAATTATCTTATAACAACTGCA AATCATTTTGTGCAGAAAAGATATGGAGGTTGGAGTTTTGGGATGAAATTGACTAACGACCTTCGTTTTG ATGTGACAGCTGTCCCTGACAATAGAACTCTTGCTAAGGTGTGGTACGATCCGGAAGGCTACCATTCCCT CCCAGCTTACCTCAACAGCCTCAACAACTTCCTCCTGAGGGTTAACATGTCAGAGTACGATGCTGCCCGA CACGGGATCATCATGTATAGCCACCCTTATCCAGGGGTACAAGACCAGGAACAAGCCACAATCAGCAGTT TAATCGATATTTTAGTGGCGCTGTCCATCCTGATGGGCTACTCTGTCACCACTGCCAGCTTTGTCACCTA CATTGTCAGGGAACATCAGACCAAAGCCAAGCAGTTGCAGCACATTTCTGGCATCGGTGTGACGTGCTAC TGGGTGACAAATTTCATCTATGACATGGTCTTCTATTTGGTTCCTGTAGCATTTTCAATTGGTGTCATCG CAATTTTCAAGCTTCCTGCCTTCTACAGTGGAAACAATCTAGGCGCTGTATCCCTCCTACTTTTGCTGTT TGGCTATGCAACATTTTCTTGGATGTACCTGCTGGCTGGCCTCTTCCACGAAACAGGAATGGCCTTCATC ACTTATGTGTGTGTCAACCTGTTCTTTGGTATCAATTCCATCGTTTCCCTGTCGGTGGTATACTTCCTTT CCAAGGAAAAGCCTAATGACCCGACGCTAGAACTTATTTCTGAAACTCTCAAGCGGATTTTCCTGATCTT CCCCCAATTCTGTTTTGGCTACGGTTTGATTGAACTTTCTCAGCAACAGGCAGTCTTGGATTTCTTAAAA GCCTACGGTGTGGAATATCCAAGCGAGACCTTTGAGATGGATAAGCTGGGGGCAATGTTTGTGGCCTTGG TTTCTCAGGGCACCATGTTTTTCTTGTTGCGACTCCTCATCAATGAGTGGCTGATAAAGAAGCTCAGGCT CTTCTTCAGGAAATTTACATCTTCGCCTATCATGGAGACAGTAGATGAAGATGAAGATGTGCGGGCTGAG AGATTTCGAGTTGAGAGTGGTGCAGCTGAGTTTGACCTGGTCCAGCTCCATCGTCTCACAAAGACCTACC AGCTGATCCACAAGAAGATTATAGCTGTCAACAACATCAGCCTGGGGATACCTGCTGGAGAGTGTTTTGG CCTCCTTGGTGTGAACGGAGCAGGGAAAACCACTATATTTAAGATGCTGACTGGAGACATAATTCCTTCC AGTGGAAACATTCTGATCAGAAACAAGAGCGGATCCCTGGGACATGTGGATTCTCACAGCTCTTTGGTTG GCTACTGCCCTCAGGAGGACGCTTTAGATGACCTAGTAACTGTGGAAGAGCACTTGTATTTCTATGCCAG AGTGCATGGGATTCCAGAGAAGGACATCAAAGACACTGTTCATAAACTCCTTAGGAGACTTCACTTGATG GCCTACAAGGACAGATCTACCTCCATGTGCAGTTACGGCACAAAAAGGAAGCTGTCCACTGCCCTGGCCT TGATAGGGAAACCGTCCATCCTCCTGCTGGATGAGCCAAGCTCTGGGATGGATCCCAAGTCAAAACGACA CCTCTGGAGGATCATTTCAGAAGAGGTGCAGAACAAATGCTCTGTCATCCTCACATCTCACAGCATGGAA GAATGTGAAGCTCTTTGTACCCGGCTGGCCATTATGGTGAACGGAAGGTTCCAGTGCATTGGGTCTCTGC AACACATAAAAAGCAGGTTTGGACGTGGATTTACTGTCAAGGTTCACTTGAAAAATAATAAAGTGAGCAT GGAAACCCTCACGAAGTTTATGCAGCTACACTTTCCAAAAACATACTTAAAAGATCAGCACCTGAGCATG CTGGAGTACCACGTGCCAGTCACAGCAGGAGGGGTCGCAAACATCTTTGACCTTCTGGAAACCAACAAGA CTGCTTTAAATATCACAAATTTCCTCGTGAGCCAGACCACTTTGGAGGAGGTTTTCATCAACTTTGCCAA AGACCAAAAATCCTACGAAAATGTTGATACCAGTAGCCAAGGTTCCACCATAAGTGTAGACTCACAGGAA GATCAACTAGATTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175210
Insert Size	7788 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175210.3, NP_780419.2
RefSeq Size	8323 bp
RefSeq ORF	7788 bp
Locus ID	74591
UniProt ID	E9Q876
Gene Summary	Transports lipids such as glucosylceramides from the outer to the inner leaflet of lamellar granules (LGs) membrane, whereby the lipids are finally transported to the keratinocyte periphery via the trans-Golgi network and LGs and released to the apical surface of the granular keratinocytes to form lipid lamellae in the stratum corneum of the epidermis, which is essential for skin barrier function (PubMed:18957418, PubMed:27551807, PubMed:24293640, PubMed:20489143, PubMed:18802465). In the mean time, participates in the transport of the lamellar granules-associated proteolytic enzymes, in turns regulates desquamation and keratinocyte differentiation (PubMed:27551807, PubMed:20489143). Furthermore, is essential for the regulation of cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux from macrophages through interaction with NR1H2 and ABCA1 (PubMed:18802465, PubMed:23931754). Plays pleiotropic roles in regulating glucose stimulated insulin secretion from beta cells, regulating the morphology and fusion of insulin granules, lipid raft abundance and the actin cytoskeleton (PubMed:32072744). Also involved in lung surfactant biogenesis (PubMed:18632686).[UniProtKB/Swiss-Prot Function]

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