Usp9x (NM_009481) Mouse Untagged Clone

CAT#: MC225357

Usp9x (untagged) - Mouse ubiquitin specific peptidase 9, X chromosome (Usp9x), (10ug)



  "NM_009481" in other vectors (2)

CNY 40,950.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; Dffrx; FAF-X; Fafl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225357 representing NM_009481
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCCACGACTCGTGGCTCTCCAGTTGGAGGGAATGACAACCAGGGCCAAGCCCCTGATGGACAGT
CTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCGTCCAATGAAAATTCCCCTGCAACTCC
TCCTGATGAGCAAGGTCAAGGTGATGCTCCTCCACAGATTGAAGATGAGGAACCTGCATTTCCACACACT
GACTTGGCAAAGTTAGATGATATGATCAACAGGCCTCGATGGGTTGTTCCAGTTTTGCCGAAAGGGGAAT
TAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAGAAAGGCCTTGATGTTAAAAGTGAAGCATGTCA
GCGATTTTTCCGAGATGGGCTAACGATCTCATTTACAAAAATCCTGACAGATGAAGCAGTGAGTGGCTGG
AAGTTTGAAATTCACAGATGCATTATTAACAATACTCATCGCCTGGTGGAGCTATGTGTGGCTAAGTTAG
CCCAAGACTGGTTTCCACTTCTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTA
CAATGGTACACGTCCCTGCGAGTCAGTGTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGT
TCTCCAGACCCTCGATCACCAAAAGGTTGGCTAGTGGATCTCCTCAACAAGTTTGGCACTTTAAATGGAT
TCCAGATACTGCACGATCGTTTTATTAATGGATCAGCATTAAACGTTCAGATAATTGCAGCCCTTATTAA
ACCATTTGGACAGTGCTATGAGTTTCTTACTCTTCACACAGTGAAAAAGTACTTTCTTCCAATAATAGAA
ATGGTTCCACAGTTTTTAGAAAACTTAACTGATGAAGAGCTGAAGAAAGAAGCAAAGAATGAAGCCAAAA
ATGATGCTCTTTCAATGATTATTAAATCATTGAAGAATTTAGCTTCGCGGGTTCCTGGACAAGAAGAAAC
TGTAAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTGTTGCAAATTTCTTCTTTCAATGGA
AAGATGAATGCACTGAATGAGGTTAATAAGGTGATTTCCAGTGTGTCATACTATACCCATCGGCATGGCA
GTTCTGAGGATGAAGAGTGGCTTACAGCAGAGAGGATGGCTGAATGGATACAGCAGAACAATATTTTATC
CATAGTTTTACGAGATAGCCTTCATCAACCACAGTATGTAGAGAAACTAGAAAAGATTCTTCGCTTTGTC
ATAAAAGAAAAAGCTCTGACCTTGCAAGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAG
CCATTGTAAAGAATGTACATGATCTACTGGCCAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCA
TCTTTTTGATTGCTTTAAGGCCAGTTGGACAAATGCAAGTAAAAAGCAACGTGAAAAGCTCCTTGAGCTG
ATTCGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACATAAAGTATTGAACCTTCTGTGGAATC
TAGCTCACAGTGATGATGTGCCTGTAGATATCATGGATTTGGCTCTCAGTGCTCACATCAAAATACTAGA
TTATAGTTGCTCCCAGGACCGGGACACCCAAAAGATCCAGTGGATTGATCGCTTCATAGAAGAACTTCGC
ACAAATGACAAATGGGTCATTCCTGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTGAAGCAC
CTCAAAATTTGAGTCAAAGTCAGCGAAGTCCCCATGTATTTTATCGCCATGACTTAATCAATCAACTTCA
GCACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGAATGTAT
GGCAGAGACAATGAAGACTATGACCCACAAACTGTGAGACTGGGAAGTAGATATAGTCATGTTCAAGAAG
TCCAAGAACGGCTTAACTTTCTTAGGTTTTTATTGAAGGATGGCCAGCTGTGGCTGTGTGCTCCACAGGC
AAAACAAATATGGAAGTGCTTAGCAGAGAATGCGGTTTATCTTTGTGATCGTGAAGCCTGTTTTAAGTGG
TATTCCAAATTAATGGGAGACGAACCTGACTTAGATCCTGATATCAATAAGGACTTCTTTGAAAGTAATG
TGCTTCAGCTTGATCCTTCCCTGTTAACTGAAAATGGGATGAAGTGTTTTGAGAGATTCTTCAAAGCTGT
GAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGAAGAGCCTACATGATGGATGATTTGGAATTGATAGGA
TTAGACTATCTTTGGAGAGTTGTAATTCAGAGTAATGATGATATTGCCTGCCGAGCTATTGATCTCCTTA
AAGAGATATACACAAACCTTGGTCCAAGGCTGCAGGTCAATCAGGTGGTGATCCATGAAGACTTCATTCA
GTCTTGCTTTGATCGTTTGAAAGCCTCTTATGACACATTGTGTGTTTTGGATGGTGACAAAGACAGTATT
AATTGTGCAAGACAGGAAGCTGTTCGAATGGTCCGAGTGTTAACTGTTCTAAGAGAATATATAAATGAAT
GTGACAGTGATTATCATGAAGAAAGAACAATTTTGCCTATGTCAAGAGCTTTCCGTGGTAAACACCTCTC
TTTTATAGTTCGATTTCCAAACCAGGGCAGGCAAGTAGATGACTTGGAGGTTTGGTCTCATACAAATGAT
ACCATTGGTTCAGTTCGAAGATGTATACTCAATCGTATTAAAGCCAATGTAGCTCATACAAAAATTGAAC
TCTTTGTGGGTGGTGAGCTTATTGATCCTGGAGATGACCGAAAGTTGATTGGACAATTAAACCTAAAAGA
TAAATCACTAATTACAGCCAAACTTACACAAATAAGCTCCAATATGCCTTCAAGTCCTGATAGCTCCTCT
GATTCCTCAACTGGATCTCCTGGAAATCATGGTAATCATTACAGTGATGGCCCCAATCCAGAAGTGGAAA
GTTGTTTGCCTGGAGTGATAATGTCACTTCATCCCAGATACATCTCTTTCCTTTGGCAAGTTGCAGACTT
AGGGAGCAGCCTAAATATGCCACCTCTTAGAGATGGAGCAAGAGTGCTTATGAAACTTATGCCGCCAGAT
AGTACGACAATAGAAAAGTTAAGAGCTATTTGTTTGGACCATGCCAAACTTGGAGAAAGCAGCCTTAGTC
CATCTCTTGACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATACCTAACAGAGGTAGTCTATGC
CTTGTTAATGCCCGCTGGTGCACCCTTGACTGATGATTCCTCGGATTTTCAGTTTCACTTCTTGAAAAGT
GGTGGTCTGCCCCTTGTCCTGAGTATGCTAACCAGAAATAACTTCCTACCAAATGCAGATATGGAAACTC
GAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAACTGTTACTAACTGCCATTGGCTATGGCCATGT
TCGTGCTGTGGCAGAAGCTTGTCAACCAGGTGTAGAAGGCGTGAATCCTATGACATCGGTCAACCAAGTA
ACTCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAACCCATCATCTGAATGCATGC
TTAGAAACGTGTCTGTTCGCCTTGCTCAGCAGATTTCTGATGAGGCTTCAAGATATATGCCTGATATTTG
TGTAATTAGAGCTATACAAAAAATTATTTGGACATCAGGATGTGGAGGATTACAGCTGGTATTCAGCCCA
AATGAAGAAGTGACAAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACCTCGAAGATGAACAGG
TTTGCTGTGAAGCATTGGAGGTGATGACACTGTGTTTCGCCTTGATTCCAACAGCCTTAGATGCTCTAAG
TAAAGAAAAAGCTTGGCAGACATTTATTATTGACTTACTGTTGCACTGTCACAGCAAAACTGTTCGTCAA
GTGGCACAGGAACAATTCTTCTTAATGTGCACCAGATGTTGCATGGGGCACAGGCCTCTACTTTTCTTCA
TTACGCTGCTCTTTACTGTTTTGGGGAGCACAGCCAGAGAGAGGGCTAAACACTCAGGCGACTACTTTAC
TCTTTTAAGACATCTTCTTAATTACGCCTACAACAGTAACATTAATGTGCCCAATGCTGAAGTTCTTCTA
AATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGGACAGGTGAAACAGGTGTTGAGG
AGACAATCTTAGAAGGACACCTTGGGGTTACAAAGGAGCTACTGGCTTTTCAGACTCCTGAGAAAAAGTT
TCATATTGGTTGTGAAAAGGGAGGTGCTAATCTCATTAAAGAATTAATCGACGACTTCATCTTTCCCGCA
TCCAATGTTTACCTGCAGTATATGAGAAATGGAGAACTCCCTGCTGAGCAGGCCATTCCTGTGTGTGGTT
CACCAGCCACAATTAATGCTGGTTTTGAGTTACTTGTGGCATTAGCTGTTGGCTGTGTGAGGAACCTCAA
ACAGATAGTAGATTCTTTGACTGAAATGTATTACATTGGTACAGCAATAACTACTTGTGAAGCACTTACT
GAGTGGGAATACCTGCCACCCGTTGGACCCCGTCCACCAAAAGGATTTGTGGGGCTGAAAAATGCTGGTG
CTACTTGTTACATGAACTCTGTGATCCAGCAACTCTATATGATCCCCTCTATCAGGAATGGTATTCTTGC
CATAGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAACGAGAGCAAT
GTTGATCCCAGGGATGATGTGTTTGGATATCCTCAACAATTTGAAGACAAACCACCACTGAGTAAAACAG
AAGATAGGAAAGAGTACAATATTGGTGTTCTAAGACACCTTCAGGTTATCTTTGGCCATTTAGCTGCTTC
TCGACTACAGTACTATGTGCCTAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTCAATCTT
CGTGAACAACATGATGCTTTAGAGTTCTTTAATTCTTTGGTGGATAGTTTAGATGAAGCTTTAAAAGCCT
TAGGGCATCCAGCTATGCTAAGTAAAGTCTTGGGAGGTTCCTTTGCTGATCAGAAAATTTGCCAAGGCTG
CCCACATAGGTACGAATGTGAAGAATCTTTTACGACTCTGAATGTAGACATTAGAAACCACCAAAATCTT
CTGGATTCTTTGGAACAGTATGTCAAAGGAGACTTACTAGAAGGAGCAAATGCTTATCATTGTGAAAAAT
GCAATAAAAAGGTTGATACTGTAAAGCGCTTGCTAATTAAAAAATTGCCTCCTGTTCTTGCTATCCAACT
AAAACGATTCGATTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTACTTTGAATTTCCTCGA
GAGTTAGACATGGAACCTTACACAGTGGCAGGTGTTGCTAAACTGGAGGGAGATAATGTCAACCCAGAAA
GTCAGCTGATACAACAGAATGAGCAGTCTGAAAGCGAGAAAGCAGGAAGCACAAAATACAGACTTGTGGG
TGTGCTTGTGCACAGTGGTCAAGCAAGTGGCGGACATTACTATTCTTACATCATTCAGAGGAATGGAGGG
GATGGTGAAAAAAATCGTTGGTATAAATTTGATGATGGAGATGTAACGGAATGTAAAATGGATGACGATG
AAGAAATGAAAAACCAGTGTTTTGGGGGAGAGTACATGGGAGAAGTATTTGATCACATGATGAAGCGCAT
GTCGTACAGGCGTCAGAAAAGATGGTGGAATGCATATATACTTTTTTATGAACGAATGGACACAATAGGT
CATGATGATGAGGTGATAAGATACATATCAGAGATTGCTATCACCACAAGGCCCCATCAGATTGTCATGC
CATCCGCCATTGAAAGAAGTGTTCGGAAGCAGAATGTTCAGTTCATGCACAACCGAATGCAGTACAGTCT
GGAATATTTTCAGTTTATGAAAAAGCTACTGACATGTAATGGTGTTTACTTAAACCCTCCTCCAGGGCAA
GATCACCTGTCTCCTGAAGCAGAAGAAATCACTATGATTAGTATTCAGCTTGCTGCTAGGTTCCTTTTTA
CTACAGGATTTCACACAAAGAAAATAGTCCGAGGATCTGCCAGTGATTGGTATGATGCATTGTGTATTCT
CCTGCGTCACAGCAAGAACGTGCGGTTTTGGTTTGCTCACAATGTCCTTTTTAATGTTTCAAATCGATTC
TCTGAGTACCTTTTGGAATGCCCTAGTGCAGAAGTGAGAGGTGCATTTGCAAAGCTCATAGTTTTCATTG
CACATTTTTCCTTACAAGACGGGCCTTGTCCTTCACCTTTTGCATCTCCTGGACCTTCTAGTCAGGCTTA
TGACAACTTAAGTTTGAGTGACCACTTACTAAGAGCAGTACTAAATCTCTTGCGGAGGGAGGTTTCAGAG
CATGGACGTCATTTACAGCAGTATTTCAACTTGTTCGTAATGTATGCCAATTTAGGAGTGGCAGAGAAAA
CACAGCTGCTCAAACTGAGTGTACCTGCTACCTTTATGCTTGTGTCCTTAGATGAAGGTCCTGGCCCTCC
AATTAAATATCAGTATGCTGAATTAGGCAAATTATACTCAGTAGTGTCACAGCTCATCCGCTGTTGCAAT
GTCTCTTCAAGAATGCAGTCTTCGATCAATGGTAATCCTTCTCTTCCAAATCCTTTTGGTGATCCTAACT
TATCACAACCTATAATGCCAATTCAACAAAATGTGGTAGACATTTTATTTGTGAGAACAAGTTATGTGAA
GAAAATTATTGAAGACTGCAGTAACTCTGATGAGACCGTCAAATTGCTTCGCTTTTGCTGCTGGGAGAAT
CCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTTTGGCAGGTTGCATATTCTTATACTTATGAACTCC
GGCCCTATTTGGATCTGCTTTTACAAATCTTACTGATTGAAGACTCTTGGCAGACTCACAGAATTCATAA
TGCCCTTAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATTCAGCGTTCTAAGAATCACTAT
CAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCCCTCTTTAGCAGTTGTCCTGTTGCTTACCAAA
TCCTGCAGGGCAATGGAGATCTTAAAAGGAAATGGACCTGGGCAGTGGAGTGGCTTGGAGATGAACTTGA
AAGAAGACCATACACTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCTCCAGTGCAAAGCAATGAA
ACATCAAATGGTTATTTCTTAGAGAGATCACACAGCGCTAGGATGACACTTGCAAAAGCTTGTGAACTCT
GTCCTGAGGAGGAACCAGATGACCAAGATGCCCCAGATGAGCATGAATCACCTCCACCAGAAGATGCTCC
TTTATACCCTCATTCCCCTGGATCTCAATATCAGCAGAATAACCATGTGCATGGACAGCCATATACAGGC
CCAGCAGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCGGTG
AAGAAGTGTCCCCTCCTCAGACCAAGGATCAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009481
Insert Size 7665 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009481.2, NP_033507.2
RefSeq Size 11903 bp
RefSeq ORF 7665 bp
Locus ID 22284
UniProt ID P70398
Gene Summary Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres (By similarity). Involved in axonal growth and neuronal cell migration (By similarity) (PubMed:24607389). Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity (PubMed:29626158).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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