Usp9x (NM_009481) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730589N07Rik; AA407302; AA407699; AL022658; AL022749; Dffrx; FAF-X; Fafl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225357 representing NM_009481 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCACGACTCGTGGCTCTCCAGTTGGAGGGAATGACAACCAGGGCCAAGCCCCTGATGGACAGT CTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCGTCCAATGAAAATTCCCCTGCAACTCC TCCTGATGAGCAAGGTCAAGGTGATGCTCCTCCACAGATTGAAGATGAGGAACCTGCATTTCCACACACT GACTTGGCAAAGTTAGATGATATGATCAACAGGCCTCGATGGGTTGTTCCAGTTTTGCCGAAAGGGGAAT TAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAGAAAGGCCTTGATGTTAAAAGTGAAGCATGTCA GCGATTTTTCCGAGATGGGCTAACGATCTCATTTACAAAAATCCTGACAGATGAAGCAGTGAGTGGCTGG AAGTTTGAAATTCACAGATGCATTATTAACAATACTCATCGCCTGGTGGAGCTATGTGTGGCTAAGTTAG CCCAAGACTGGTTTCCACTTCTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTA CAATGGTACACGTCCCTGCGAGTCAGTGTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGT TCTCCAGACCCTCGATCACCAAAAGGTTGGCTAGTGGATCTCCTCAACAAGTTTGGCACTTTAAATGGAT TCCAGATACTGCACGATCGTTTTATTAATGGATCAGCATTAAACGTTCAGATAATTGCAGCCCTTATTAA ACCATTTGGACAGTGCTATGAGTTTCTTACTCTTCACACAGTGAAAAAGTACTTTCTTCCAATAATAGAA ATGGTTCCACAGTTTTTAGAAAACTTAACTGATGAAGAGCTGAAGAAAGAAGCAAAGAATGAAGCCAAAA ATGATGCTCTTTCAATGATTATTAAATCATTGAAGAATTTAGCTTCGCGGGTTCCTGGACAAGAAGAAAC TGTAAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTGTTGCAAATTTCTTCTTTCAATGGA AAGATGAATGCACTGAATGAGGTTAATAAGGTGATTTCCAGTGTGTCATACTATACCCATCGGCATGGCA GTTCTGAGGATGAAGAGTGGCTTACAGCAGAGAGGATGGCTGAATGGATACAGCAGAACAATATTTTATC CATAGTTTTACGAGATAGCCTTCATCAACCACAGTATGTAGAGAAACTAGAAAAGATTCTTCGCTTTGTC ATAAAAGAAAAAGCTCTGACCTTGCAAGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAG CCATTGTAAAGAATGTACATGATCTACTGGCCAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCA TCTTTTTGATTGCTTTAAGGCCAGTTGGACAAATGCAAGTAAAAAGCAACGTGAAAAGCTCCTTGAGCTG ATTCGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACATAAAGTATTGAACCTTCTGTGGAATC TAGCTCACAGTGATGATGTGCCTGTAGATATCATGGATTTGGCTCTCAGTGCTCACATCAAAATACTAGA TTATAGTTGCTCCCAGGACCGGGACACCCAAAAGATCCAGTGGATTGATCGCTTCATAGAAGAACTTCGC ACAAATGACAAATGGGTCATTCCTGCACTGAAACAAATTAGAGAAATTTGTAGTTTGTTTGGTGAAGCAC CTCAAAATTTGAGTCAAAGTCAGCGAAGTCCCCATGTATTTTATCGCCATGACTTAATCAATCAACTTCA GCACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGAATGTAT GGCAGAGACAATGAAGACTATGACCCACAAACTGTGAGACTGGGAAGTAGATATAGTCATGTTCAAGAAG TCCAAGAACGGCTTAACTTTCTTAGGTTTTTATTGAAGGATGGCCAGCTGTGGCTGTGTGCTCCACAGGC AAAACAAATATGGAAGTGCTTAGCAGAGAATGCGGTTTATCTTTGTGATCGTGAAGCCTGTTTTAAGTGG TATTCCAAATTAATGGGAGACGAACCTGACTTAGATCCTGATATCAATAAGGACTTCTTTGAAAGTAATG TGCTTCAGCTTGATCCTTCCCTGTTAACTGAAAATGGGATGAAGTGTTTTGAGAGATTCTTCAAAGCTGT GAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGAAGAGCCTACATGATGGATGATTTGGAATTGATAGGA TTAGACTATCTTTGGAGAGTTGTAATTCAGAGTAATGATGATATTGCCTGCCGAGCTATTGATCTCCTTA AAGAGATATACACAAACCTTGGTCCAAGGCTGCAGGTCAATCAGGTGGTGATCCATGAAGACTTCATTCA GTCTTGCTTTGATCGTTTGAAAGCCTCTTATGACACATTGTGTGTTTTGGATGGTGACAAAGACAGTATT AATTGTGCAAGACAGGAAGCTGTTCGAATGGTCCGAGTGTTAACTGTTCTAAGAGAATATATAAATGAAT GTGACAGTGATTATCATGAAGAAAGAACAATTTTGCCTATGTCAAGAGCTTTCCGTGGTAAACACCTCTC TTTTATAGTTCGATTTCCAAACCAGGGCAGGCAAGTAGATGACTTGGAGGTTTGGTCTCATACAAATGAT ACCATTGGTTCAGTTCGAAGATGTATACTCAATCGTATTAAAGCCAATGTAGCTCATACAAAAATTGAAC TCTTTGTGGGTGGTGAGCTTATTGATCCTGGAGATGACCGAAAGTTGATTGGACAATTAAACCTAAAAGA TAAATCACTAATTACAGCCAAACTTACACAAATAAGCTCCAATATGCCTTCAAGTCCTGATAGCTCCTCT GATTCCTCAACTGGATCTCCTGGAAATCATGGTAATCATTACAGTGATGGCCCCAATCCAGAAGTGGAAA GTTGTTTGCCTGGAGTGATAATGTCACTTCATCCCAGATACATCTCTTTCCTTTGGCAAGTTGCAGACTT AGGGAGCAGCCTAAATATGCCACCTCTTAGAGATGGAGCAAGAGTGCTTATGAAACTTATGCCGCCAGAT AGTACGACAATAGAAAAGTTAAGAGCTATTTGTTTGGACCATGCCAAACTTGGAGAAAGCAGCCTTAGTC CATCTCTTGACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATACCTAACAGAGGTAGTCTATGC CTTGTTAATGCCCGCTGGTGCACCCTTGACTGATGATTCCTCGGATTTTCAGTTTCACTTCTTGAAAAGT GGTGGTCTGCCCCTTGTCCTGAGTATGCTAACCAGAAATAACTTCCTACCAAATGCAGATATGGAAACTC GAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAACTGTTACTAACTGCCATTGGCTATGGCCATGT TCGTGCTGTGGCAGAAGCTTGTCAACCAGGTGTAGAAGGCGTGAATCCTATGACATCGGTCAACCAAGTA ACTCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAACCCATCATCTGAATGCATGC TTAGAAACGTGTCTGTTCGCCTTGCTCAGCAGATTTCTGATGAGGCTTCAAGATATATGCCTGATATTTG TGTAATTAGAGCTATACAAAAAATTATTTGGACATCAGGATGTGGAGGATTACAGCTGGTATTCAGCCCA AATGAAGAAGTGACAAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACCTCGAAGATGAACAGG TTTGCTGTGAAGCATTGGAGGTGATGACACTGTGTTTCGCCTTGATTCCAACAGCCTTAGATGCTCTAAG TAAAGAAAAAGCTTGGCAGACATTTATTATTGACTTACTGTTGCACTGTCACAGCAAAACTGTTCGTCAA GTGGCACAGGAACAATTCTTCTTAATGTGCACCAGATGTTGCATGGGGCACAGGCCTCTACTTTTCTTCA TTACGCTGCTCTTTACTGTTTTGGGGAGCACAGCCAGAGAGAGGGCTAAACACTCAGGCGACTACTTTAC TCTTTTAAGACATCTTCTTAATTACGCCTACAACAGTAACATTAATGTGCCCAATGCTGAAGTTCTTCTA AATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGGACAGGTGAAACAGGTGTTGAGG AGACAATCTTAGAAGGACACCTTGGGGTTACAAAGGAGCTACTGGCTTTTCAGACTCCTGAGAAAAAGTT TCATATTGGTTGTGAAAAGGGAGGTGCTAATCTCATTAAAGAATTAATCGACGACTTCATCTTTCCCGCA TCCAATGTTTACCTGCAGTATATGAGAAATGGAGAACTCCCTGCTGAGCAGGCCATTCCTGTGTGTGGTT CACCAGCCACAATTAATGCTGGTTTTGAGTTACTTGTGGCATTAGCTGTTGGCTGTGTGAGGAACCTCAA ACAGATAGTAGATTCTTTGACTGAAATGTATTACATTGGTACAGCAATAACTACTTGTGAAGCACTTACT GAGTGGGAATACCTGCCACCCGTTGGACCCCGTCCACCAAAAGGATTTGTGGGGCTGAAAAATGCTGGTG CTACTTGTTACATGAACTCTGTGATCCAGCAACTCTATATGATCCCCTCTATCAGGAATGGTATTCTTGC CATAGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAACGAGAGCAAT GTTGATCCCAGGGATGATGTGTTTGGATATCCTCAACAATTTGAAGACAAACCACCACTGAGTAAAACAG AAGATAGGAAAGAGTACAATATTGGTGTTCTAAGACACCTTCAGGTTATCTTTGGCCATTTAGCTGCTTC TCGACTACAGTACTATGTGCCTAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTCAATCTT CGTGAACAACATGATGCTTTAGAGTTCTTTAATTCTTTGGTGGATAGTTTAGATGAAGCTTTAAAAGCCT TAGGGCATCCAGCTATGCTAAGTAAAGTCTTGGGAGGTTCCTTTGCTGATCAGAAAATTTGCCAAGGCTG CCCACATAGGTACGAATGTGAAGAATCTTTTACGACTCTGAATGTAGACATTAGAAACCACCAAAATCTT CTGGATTCTTTGGAACAGTATGTCAAAGGAGACTTACTAGAAGGAGCAAATGCTTATCATTGTGAAAAAT GCAATAAAAAGGTTGATACTGTAAAGCGCTTGCTAATTAAAAAATTGCCTCCTGTTCTTGCTATCCAACT AAAACGATTCGATTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTACTTTGAATTTCCTCGA GAGTTAGACATGGAACCTTACACAGTGGCAGGTGTTGCTAAACTGGAGGGAGATAATGTCAACCCAGAAA GTCAGCTGATACAACAGAATGAGCAGTCTGAAAGCGAGAAAGCAGGAAGCACAAAATACAGACTTGTGGG TGTGCTTGTGCACAGTGGTCAAGCAAGTGGCGGACATTACTATTCTTACATCATTCAGAGGAATGGAGGG GATGGTGAAAAAAATCGTTGGTATAAATTTGATGATGGAGATGTAACGGAATGTAAAATGGATGACGATG AAGAAATGAAAAACCAGTGTTTTGGGGGAGAGTACATGGGAGAAGTATTTGATCACATGATGAAGCGCAT GTCGTACAGGCGTCAGAAAAGATGGTGGAATGCATATATACTTTTTTATGAACGAATGGACACAATAGGT CATGATGATGAGGTGATAAGATACATATCAGAGATTGCTATCACCACAAGGCCCCATCAGATTGTCATGC CATCCGCCATTGAAAGAAGTGTTCGGAAGCAGAATGTTCAGTTCATGCACAACCGAATGCAGTACAGTCT GGAATATTTTCAGTTTATGAAAAAGCTACTGACATGTAATGGTGTTTACTTAAACCCTCCTCCAGGGCAA GATCACCTGTCTCCTGAAGCAGAAGAAATCACTATGATTAGTATTCAGCTTGCTGCTAGGTTCCTTTTTA CTACAGGATTTCACACAAAGAAAATAGTCCGAGGATCTGCCAGTGATTGGTATGATGCATTGTGTATTCT CCTGCGTCACAGCAAGAACGTGCGGTTTTGGTTTGCTCACAATGTCCTTTTTAATGTTTCAAATCGATTC TCTGAGTACCTTTTGGAATGCCCTAGTGCAGAAGTGAGAGGTGCATTTGCAAAGCTCATAGTTTTCATTG CACATTTTTCCTTACAAGACGGGCCTTGTCCTTCACCTTTTGCATCTCCTGGACCTTCTAGTCAGGCTTA TGACAACTTAAGTTTGAGTGACCACTTACTAAGAGCAGTACTAAATCTCTTGCGGAGGGAGGTTTCAGAG CATGGACGTCATTTACAGCAGTATTTCAACTTGTTCGTAATGTATGCCAATTTAGGAGTGGCAGAGAAAA CACAGCTGCTCAAACTGAGTGTACCTGCTACCTTTATGCTTGTGTCCTTAGATGAAGGTCCTGGCCCTCC AATTAAATATCAGTATGCTGAATTAGGCAAATTATACTCAGTAGTGTCACAGCTCATCCGCTGTTGCAAT GTCTCTTCAAGAATGCAGTCTTCGATCAATGGTAATCCTTCTCTTCCAAATCCTTTTGGTGATCCTAACT TATCACAACCTATAATGCCAATTCAACAAAATGTGGTAGACATTTTATTTGTGAGAACAAGTTATGTGAA GAAAATTATTGAAGACTGCAGTAACTCTGATGAGACCGTCAAATTGCTTCGCTTTTGCTGCTGGGAGAAT CCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTTTGGCAGGTTGCATATTCTTATACTTATGAACTCC GGCCCTATTTGGATCTGCTTTTACAAATCTTACTGATTGAAGACTCTTGGCAGACTCACAGAATTCATAA TGCCCTTAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATTCAGCGTTCTAAGAATCACTAT CAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCCCTCTTTAGCAGTTGTCCTGTTGCTTACCAAA TCCTGCAGGGCAATGGAGATCTTAAAAGGAAATGGACCTGGGCAGTGGAGTGGCTTGGAGATGAACTTGA AAGAAGACCATACACTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCTCCAGTGCAAAGCAATGAA ACATCAAATGGTTATTTCTTAGAGAGATCACACAGCGCTAGGATGACACTTGCAAAAGCTTGTGAACTCT GTCCTGAGGAGGAACCAGATGACCAAGATGCCCCAGATGAGCATGAATCACCTCCACCAGAAGATGCTCC TTTATACCCTCATTCCCCTGGATCTCAATATCAGCAGAATAACCATGTGCATGGACAGCCATATACAGGC CCAGCAGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCGGTG AAGAAGTGTCCCCTCCTCAGACCAAGGATCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009481
Insert Size	7665 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009481.2, NP_033507.2
RefSeq Size	11903 bp
RefSeq ORF	7665 bp
Locus ID	22284
UniProt ID	P70398
Gene Summary	Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres (By similarity). Involved in axonal growth and neuronal cell migration (By similarity) (PubMed:24607389). Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity (PubMed:29626158).[UniProtKB/Swiss-Prot Function]

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