Mtor (NM_020009) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610315D21Rik; AI327068; flat; FRAP; Frap1; FRAP2; RAFT1; RAPT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225356 representing NM_020009 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGGGACGGGTCCTGCCGTGGCCACCGCCAGTGCCGCCACATCTAGCAACGTGAGCGTCCTGCAGC AGTTCGCCAGTGGACTGAAGAGCCGGAATGAGGAGACCAGGGCCAAAGCAGCCAAGGAGCTCCAGCACTA CGTCACCATGGAGCTTCGAGAGATGAGTCAGGAGGAGTCTACTCGCTTCTATGACCAGCTGAACCATCAC ATTTTTGAACTGGTTTCCAGCTCAGATGCCAATGAGAGGAAGGGTGGCATCTTGGCCATCGCCAGCCTCA TAGGAGTGGAAGGTGGGAATTCCACCAGAATTGGCAGATTTGCCAACTACCTTCGAAACCTCCTCCCCTC AAGCGATCCAGTTGTCATGGAAATGGCGTCCAAGGCCATTGGCCGCCTGGCGATGGCAGGGGACACTTTC ACTGCTGAATATGTGGAGTTTGAAGTGAAGCGAGCCTTGGAGTGGCTGGGTGCTGACCGAAATGAGGGCC GGAGACATGCCGCTGTCCTCGTTCTCCGTGAGCTGGCCATCAGTGTCCCCACCTTCTTCTTCCAGCAAGT TCAGCCCTTCTTTGACAACATTTTTGTGGCTGTGTGGGACCCCAAGCAGGCCATCCGGGAAGGCGCTGTA GCGGCCCTTCGTGCCTGTCTGATTCTCACCACGCAGCGGGAACCAAAGGAAATGCAGAAGCCTCAGTGGT ACCGGCACACATTTGAAGAAGCAGAGAAAGGTTTTGATGAGACCCTGGCCAAAGAGAAGGGTATGAATCG AGATGATCGAATCCACGGAGCCTTGCTGATCCTCAACGAGCTAGTTCGTATCAGCAGCATGGAGGGAGAG CGTCTGAGAGAAGAGATGGAGGAGATCACCCAGCAGCAGCTGGTGCATGACAAGTACTGCAAAGACCTCA TGGGCTTCGGGACCAAGCCTCGGCACATCACGCCCTTCACCAGTTTCCAGGCTGTGCAGCCCCAGCAGCC GAACGCCTTGGTGGGACTGCTGGGGTACAGCTCCCCTCAAGGCCTGATGGGATTTGGGACGTCCCCCAGC CCTGCCAAGTCCACTCTGGTGGAAAGCCGCTGTTGCAGAGACTTGATGGAAGAGAAATTTGATCAGGTGT GCCAGTGGGTGCTGAAGTGCAGGAGCAGCAAGAACTCGCTGATCCAGATGACAATCCTTAACCTGCTGCC CCGCCTGGCTGCATTCCGACCGTCCGCCTTCACAGATACCCAGTACCTCCAGGACACCATGAACCATGTC CTGAGCTGTGTCAAGAAGGAGAAGGAACGGACTGCGGCGTTCCAGGCCCTGGGGCTGCTTTCTGTGGCCG TGAGGTCGGAGTTTAAGGTCTACTTGCCCCGTGTACTTGACATCATCCGAGCAGCGCTTCCTCCAAAGGA CTTTGCCCACAAGAGGCAGAAAACCGTGCAGGTGGATGCCACCGTATTCACGTGCATCAGCATGTTGGCA CGAGCAATGGGGCCGGGCATCCAGCAGGACATCAAGGAGCTGCTGGAGCCCATGTTGGCAGTGGGCCTGA GCCCCGCGCTCACTGCTGTGCTCTATGACCTGAGCCGGCAGATTCCACAGCTGAAGAAAGATATTCAGGA CGGCCTTCTGAAGATGCTGTCCCTGGTCCTTATGCACAAACCCCTCCGGCACCCAGGCATGCCCAAAGGC CTGGCTCACCAGCTGGCTTCCCCTGGTCTCACCACCCTCCCTGAGGCCAGCGACGTGGCCAGCATCACTC TTGCCCTTCGAACCCTTGGCAGCTTTGAATTTGAAGGCCACTCTCTGACCCAGTTCGTCCGACACTGCGC AGATCACTTCCTGAACAGCGAGCACAAGGAGATCCGCATGGAAGCTGCTCGCACCTGCTCCCGCCTGCTC ACACCCTCCATCCACCTCATCAGCGGCCATGCCCACGTGGTTAGCCAGACTGCAGTGCAGGTGGTGGCAG ATGTGCTCAGCAAGCTGCTTGTGGTTGGCATAACAGATCCTGACCCTGATATCCGCTACTGTGTCTTGGC ATCCCTGGACGAGCGCTTTGATGCCCACCTGGCCCAGGCAGAAAACTTACAAGCTCTGTTTGTGGCTCTG AATGACCAGGTCTTTGAGATCCGCGAGCTGGCCATCTGCACTGTGGGCCGACTAAGCAGCATGAACCCAG CCTTCGTCATGCCTTTCCTGCGCAAGATGCTCATCCAGATCCTGACAGAGCTGGAGCACAGCGGCATTGG GAGAATCAAGGAGCAGAGCGCCCGCATGCTGGGGCACCTGGTGTCCAACGCCCCCCGGCTCATCCGCCCC TACATGGAGCCTATCCTGAAGGCTTTAATTTTGAAACTGAAAGATCCAGACCCTGACCCAAACCCGGGCG TGATCAATAACGTGTTGGCCACTATAGGAGAACTGGCTCAGGTGAGCGGCCTGGAAATGCGGAAGTGGGT GGACGAGCTCTTTATCATCATCATGGACATGCTGCAGGACTCCTCCCTGCTGGCCAAAAGGCAGGTGGCT TTGTGGACCCTGGGACAGTTGGTGGCCAGCACTGGCTATGTGGTGGAGCCCTACAGGAAGTACCCCACTT TGCTTGAAGTGCTGCTGAATTTCCTGAAGACGGAGCAGAACCAGGGCACTCGGAGAGAGGCTATCCGAGT GTTGGGGCTCCTTGGGGCTTTGGATCCCTACAAGCACAAAGTGAACATCGGCATGATCGACCAGTCCCGG GACGCTTCCGCTGTCAGCCTGTCAGAGTCCAAGTCAAGTCAGGATTCCTCTGACTACAGCACCAGTGAAA TGCTGGTCAACATGGGAAACCTGCCCCTGGACGAGTTCTACCCCGCTGTGTCCATGGTGGCCTTGATGCG GATCTTCCGAGATCAATCCCTCTCTCACCACCACACCATGGTGGTGCAGGCCATCACCTTCATCTTCAAG TCCCTGGGGCTCAAGTGTGTGCAGTTCCTGCCCCAGGTCATGCCCACATTCCTTAATGTCATCCGAGTCT GTGATGGGGCCATCCGGGAATTTCTGTTCCAGCAGCTGGGGATGCTGGTGTCCTTTGTGAAGAGCCACAT CCGTCCCTACATGGATGAAATAGTCACTCTCATGAGAGAGTTTTGGGTCATGAACACGTCAATCCAGAGC ACAATCATTCTTCTCATTGAGCAGATTGTGGTGGCTCTCGGAGGGGAATTTAAGCTTTATCTGCCCCAGT TGATCCCACACATGCTGCGGGTCTTCATGCATGACAACAGCCAAGGCCGAATCGTCTCCATCAAGCTGTT AGCCGCGATCCAGCTGTTTGGCGCCAACCTGGATGACTATCTGCACTTGTTGTTGCCTCCGATTGTGAAA TTGTTTGATGCCCCTGAAGTCCCGCTGCCATCAAGAAAGGCAGCGCTGGAGACGGTGGACCGCCTGACAG AGTCCCTAGACTTCACTGACTACGCCTCCCGCATCATTCACCCAATAGTTCGTACGCTAGACCAGAGCCC AGAGCTGCGCTCCACAGCCATGGACACTCTGTCTTCGCTTGTCTTTCAACTGGGGAAGAAGTACCAGATC TTCATTCCAATGGTGAATAAAGTCCTCGTGCGACACCGGATCAACCACCAGCGCTATGATGTGCTTATCT GCAGAATCGTCAAGGGGTACACACTTGCTGATGAGGAAGAAGACCCTTTGATTTACCAGCATCGAATGCT AAGGAGCAGCCAGGGAGATGCCCTGGCCAGTGGACCAGTTGAGACAGGACCCATGAAGAAACTGCATGTC AGCACCATCAACCTCCAAAAGGCCTGGGGAGCTGCCAGAAGGGTCTCCAAGGACGACTGGCTGGAGTGGC TGAGGCGCTTGAGTCTGGAGCTTCTGAAGGACTCCTCATCGCCCTCCCTGCGCTCATGCTGGGCCCTGGC GCAGGCCTACAACCCCATGGCCAGGGATCTCTTCAATGCTGCCTTTGTGTCCTGCTGGTCTGAGCTGAAT GAAGACCAGCAAGATGAGCTCATCAGGAGTATTGAGTTGGCTCTCACTTCTCAAGACATTGCTGAAGTCA CACAAACCCTCCTGAACTTGGCTGAGTTCATGGAACACAGTGACAAGGGCCCCCTGCCGCTGAGAGATGA CAATGGCATCGTGCTCCTGGGTGAGAGAGCTGCCAAGTGCCGGGCATATGCCAAAGCACTGCACTACAAA GAACTGGAGTTCCAGAAAGGGCCCACGCCTGCCATACTTGAGTCCCTCATCAGCATTAACAACAAGCTCC AGCAGCCTGAGGCAGCTTCTGGGGTGTTGGAATACGCCATGAAACACTTCGGAGAGCTGGAGATCCAGGC CACCTGGTATGAGAAGCTGCATGAGTGGGAGGATGCTCTCGTGGCCTACGACAAGAAGATGGACACAAAC AAGGAAGACCCGGAGCTGATGCTGGGCCGAATGCGCTGCCTCGAGGCCTTGGGGGAATGGGGCCAGCTTC ATCAGCAGTGCTGTGAAAAGTGGACTCTGGTTAATGATGAGACCCAGGCTAAGATGGCCCGGATGGCTGC TGCTGCAGCGTGGGGTTTAGGTCAGTGGGACAGCATGGAGGAGTACACCTGCATGATCCCACGGGACACC CACGATGGAGCCTTTTACAGGGCAGTGTTGGCTCTACATCAGGATCTCTTCTCCTTGGCCCAGCAGTGCA TTGACAAGGCCAGGGACCTGCTGGATGCAGAGCTGACTGCCATGGCAGGAGAGAGCTACAGCCGAGCCTA TGGGGCCATGGTTTCTTGCCACATGCTGTCCGAGCTGGAAGAGGTTATCCAGTACAAACTTGTCCCTGAG CGTCGGGAGATCATCCGGCAGATCTGGTGGGAGAGACTGCAGGGCTGCCAGCGTATTGTTGAGGACTGGC AGAAAATCCTCATGGTCCGGTCCCTTGTGGTCAGCCCTCATGAGGACATGAGAACCTGGCTCAAGTACGC AAGCCTGTGTGGCAAGAGTGGCAGACTGGCTCTTGCTCATAAAACCTTAGTGTTGCTCTTGGGAGTTGAT CCATCTCGGCAACTTGACCATCCTCTGCCAACCGCTCACCCTCAAGTGACCTATGCCTACATGAAGAACA TGTGGAAAAGTGCTCGGAAGATTGACGCCTTCCAGCACATGCAACACTTTGTGCAGACCATGCAGCAGCA GGCCCAGCATGCCATCGCCACAGAGGACCAGCAGCACAAGCAGGAGCTGCATAAGCTCATGGCCAGGTGT TTTCTGAAACTTGGGGAGTGGCAGCTGAACCTCCAGGGCATCAACGAGAGCACCATCCCCAAGGTGCTAC AGTACTACAGTGCCGCCACAGAGCATGACCGCAGCTGGTACAAGGCTTGGCATGCATGGGCAGTGATGAA CTTCGAAGCAGTGCTACACTACAAACATCAGAACCAAGCCCGTGATGAGAAGAAGAAGCTGCGTCATGCC AGCGGGGCCAACATCACCAATGCCACCACTGCAGCCACCACTGCAGCCTCTGCTGCTGCTGCCACCAGCA CAGAGGGCAGCAACAGTGAGAGTGAAGCTGAGAGCAATGAGAACAGCCCCACCCCGTCCCCTCTGCAGAA GAAGGTCACTGAGGATTTATCCAAAACTCTCTTGTTGTACACTGTCCCTGCTGTTCAAGGCTTCTTCCGT TCTATCTCCTTGTCAAGAGGCAACAACCTCCAGGATACACTAAGAGTCCTCACCCTGTGGTTTGATTATG GTCACTGGCCAGATGTCAATGAAGCCTTGGTGGAAGGGGTGAAGGCCATACAGATTGACACTTGGTTACA GGTTATACCTCAGCTCATTGCAAGAATTGACACACCCAGACCCTTGGTGGGCCGGCTCATTCACCAGCTT CTCACAGATATTGGTCGGTACCACCCACAGGCCCTCATCTACCCCCTGACGGTGGCTTCTAAGTCTACCA CCACAGCCCGTCACAATGCAGCCAACAAGATCTTGAAGAACATGTGTGAACACAGCAACACGCTGGTCCA GCAGGCCATGATGGTGAGTGAAGAGCTGATTCGGGTAGCCATCCTCTGGCATGAGATGTGGCATGAAGGC CTGGAAGAGGCATCTCGCTTGTACTTTGGGGAGAGGAACGTGAAAGGCATGTTTGAGGTGCTGGAGCCCC TGCATGCTATGATGGAACGGGGTCCCCAGACTCTGAAGGAAACATCCTTTAATCAGGCATATGGCCGAGA TTTAATGGAGGCACAAGAATGGTGTCGAAAGTACATGAAGTCGGGGAACGTCAAGGACCTCACGCAAGCC TGGGACCTCTACTATCACGTGTTCAGACGGATCTCAAAGCAGCTACCCCAGCTCACATCCCTGGAGCTGC AGTATGTGTCCCCCAAACTTCTGATGTGCCGAGACCTTGAGTTGGCTGTGCCAGGAACATACGACCCCAA CCAGCCAATCATTCGCATTCAATCCATAGCCCCGTCTTTGCAAGTCATCACATCCAAGCAGAGGCCTCGG AAGCTGACTCTGATGGGCAGCAATGGGCATGAGTTTGTTTTCCTCCTGAAAGGCCATGAAGATCTGCGGC AGGATGAACGAGTGATGCAGCTCTTTGGCCTGGTGAACACACTCCTAGCCAATGACCCCACTTCTCTTCG CAAGAACCTCAGCATCCAGAGATACGCTGTCATCCCTCTGTCCACCAACTCGGGCCTCATTGGCTGGGTG CCCCACTGTGACACACTGCATGCCCTCATCCGGGACTACAGAGAGAAGAAGAAGATCCTGTTGAACATCG AGCATCGCATCATGCTGCGGATGGCTCCTGACTATGACCACCTGACGTTGATGCAGAAGGTAGAGGTGTT TGAGCATGCTGTCAACAACACAGCTGGGGACGACCTGGCCAAGCTACTGTGGCTAAAAAGCCCCAGCTCG GAGGTGTGGTTTGACCGAAGAACCAACTATACCCGCTCCCTGGCCGTTATGTCGATGGTCGGATACATTT TAGGCCTTGGAGACAGGCACCCATCCAATCTGATGCTGGACCGGCTGAGTGGGAAGATCCTGCACATTGA CTTTGGGGACTGCTTTGAGGTCGCTATGACCAGAGAGAAATTTCCAGAAAAGATTCCATTTAGACTAACA AGAATGTTGACCAATGCTATGGAGGTTACGGGTCTGGATGGCAACTACAGAACCACATGCCACACCGTGA TGGAAGTGCTCCGGGAACACAAGGACAGTGTCATGGCTGTGCTGGAAGCCTTTGTCTATGACCCACTGCT CAACTGGAGGCTGATGGACACAAATACCAAAGGCAATAAGCGGTCCCGGACAAGGACAGACTCCTACTCT GCCGGCCAGTCAGTAGAAATTTTGGACGGTGTAGAACTTGGAGAACCAGCCCATAAGAAAGCAGGGACCA CTGTGCCAGAATCCATCCATTCATTCATTGGAGACGGTTTGGTGAAACCAGAAGCCTTAAACAAGAAAGC TATTCAGATTATTAACAGGGTTCGAGATAAGCTCACTGGTCGGGATTTCTCTCATGATGACACTTTGGAT GTTCCAACCCAAGTGGAGCTGCTTATCAAGCAGGCAACATCTCACGAGAACCTCTGCCAGTGCTACATTG GCTGGTGTCCCTTCTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_020009
Insert Size	7650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020009.2, NP_064393.2
RefSeq Size	8612 bp
RefSeq ORF	7650 bp
Locus ID	56717
UniProt ID	Q9JLN9
Gene Summary	Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals (PubMed:15467718, PubMed:15545625, PubMed:16221682, PubMed:16915281, PubMed:16962653, PubMed:18046414, PubMed:19440205, PubMed:21659604). MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins (PubMed:15467718, PubMed:15545625, PubMed:16221682, PubMed:16915281, PubMed:16962653, PubMed:18046414, PubMed:19440205, PubMed:21659604). Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2) (PubMed:15467718, PubMed:16962653, PubMed:21659604). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis (By similarity). This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E) (By similarity). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4 (By similarity). Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex (By similarity). Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor (By similarity). In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1 (PubMed:11792863). To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A (PubMed:18046414). mTORC1 also negatively regulates autophagy through phosphorylation of ULK1 (PubMed:21258367). Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1 (PubMed:21258367). Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP (By similarity). Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions (By similarity). mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor (PubMed:21659604). Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules (By similarity). As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton (By similarity). Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1 (By similarity). mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B (By similarity). mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (By similarity). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (PubMed:19440205). Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks (PubMed:29750810).[UniProtKB/Swiss-Prot Function]

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