Igf2r (NM_010515) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI661837; CD222; CI-MPR; M6P/IGF2R; Mpr300
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225336 representing NM_010515 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCCGTTCAGCTGGGACCGGTGCCCTCCGGGCCGCGCGTCGCGCTCCTGCCGCCGCTCCTGCTGC TGCTGCTCCTGGCGGCCGCGGGCTCCGCGCAGGCCCAGGCCGTCGACTTGGACGCCCTGTGCAGTTACAC ATGGGAAGCTGTTGACTCCAAAAACAATGCAGTTTATAAGATCAACGTCTGTGGAAATGTGGGTATTTCC AGCTGTGGGCCTACAAGTGCTATCTGTATGTGTGACCTAAAGACAGAGAACTGCCGCTCAGTGGGTGACT CACTTTTGAGAAGTTCAGCCAGATCACTCCTGGAATTCAACACCACTATGGGCTGCCAGCCTTCAGATTC ACAGCACAGAATCCAGACTAGCATTACATTTCTGTGTGGGAAGACCCTGGGAACTCCTGAATTTGTAACT GCCACAGACTGTGTGCATTACTTTGAATGGAGGACTACTGCAGCCTGCAAGAAAGACATATTTAAAGCTG ATAAGGAGGTACCATGCTATGCATTTGATGACAAGTTACAGAAGCATGATTTAAACCCACTGATCAAGCT AAATGGTGGCTATCTGGTAGATGATTCTGATCCTGACACGTCTCTGTTCATCAACGTGTGTAGAGACATA GACTCCCTTCGGGACCCCAGCACACAGCTGAGAGTCTGTCCTGCTGGCACTGCTGCCTGTCTGCTGAAGG GGAACCAGGCATTTGACGTTGGCCGACCCAAGGAGGGGCTGAAGCTGTTGAGCAAGGACAGGCTCGTTCT GACTTATGTGAAGGAAGAGGGAGAAAAGCCAGACTTCTGTAATGGTCACAGCCCTGCAGTGACAGTAACA TTTGTGTGCCCATCTGAGCGGAGAGAGGGTACCATTCCTAAACTCACTGCCAAGTCCAACTGCCGTTATG AAGTTGAGTGGATCACCGAGTATGCCTGCCACAGAGATTACCTGCAAAGTGAGAGCTGCTCCCTCAGCAG TGAGCAGCATGATATTACTATTGACCTTAGTCCCTTGGCCCAATATGGAGGGTCCCCCTATGTTTCTGAT GGAAGAGAATATACGTTTTTTATCAATGTCTGTGGGGACACAAAGGTCTCACTCTGTAATAACAAAGAGG CTGCTGTTTGTCAAGAGAAAAAGGCGGATTCCACTCAAGTCAAAATAGCAGGAAGACACCAGAACCAGAC ACTCCGGTACTCAGATGGAGACCTCACCCTCATCTATTCTGGAGGTGATGAGTGTAGCTCTGGCTTCCAG AGGATGAGTGTCATAAACTTTGAGTGCAACAAAACTGCAGGTAAGGATGGGAGAGGAGAGCCTGTGTTCA CAGGTGAGGTGGACTGCACCTACTTCTTCACATGGGACACTAAATACGCCTGCATCAAAGAGAAGGAAGA CCTCCTCTGCGGGGCCATCAATGGCAAGAAACGCTATGACCTGTCTGTGTTGGCTCGTCACTCAGAATCA GAACAGAATTGGGAAGCAGTGGATGGCAGCCAGGCAGAATCAGAAAAGTATTTCTTCATCAACGTCTGTC ACCGGGTGCTGCAGGAGGGGAAGGCCAGGAACTGTCCTGAAGATGCTGCTGTGTGCGCTGTGGATAAGAA TGGGAGTAAAAACCTTGGAAAATTTGTCTCTTCTCCTACAAAAGAGAAAGGACACATACAGCTCTCTTAC ACTGATGGTGATGACTGTGGCAGTGATAAGAAAATATCAACTAACATCACACTTGTGTGCAAACCAGGTG ATCTAGAAAGTGCTCCAGTGCTAAGAGCTGCAAGATCTGATGGCTGCTTCTATGAGTTTGAGTGGCATAC GGCTGCTGCCTGTGTACTGTCGAAGACAGAAGGAGAGAACTGCACGGTCCTTGATGCCCAGGCAGGGTTT TCCTTTGATTTGTCACTCCTCACGAAGAAGAATGGTGCATATAAAGTTGAGACAGAGAAGTATGACTTCT ACATAAATGTTTGTGGCCCTGTATCCATGGATCCTTGTCAGTCAAACTCGGGGGCCTGCCAGGTAGCGAA AAGTGGTAAGTCTTGGAACTTGGGGCTGAGTAGTACTAAGCTCACCTACTACGACGGAATGATCCAGCTG AGCTACAGGAACGGCACTCCCTATAACAATGAAAAGCACACACCGAGAGCCACACTGATTACCTTCCTCT GTGACCGTGACGCTGGAGTGGGCTTCCCAGAATATCAGGAAGAGGACAACTCTACCTACAACTTCCGGTG GTACACCAGTTATGCCTGCCCAGAAGAACCCCTGGAGTGCATGGTGACAGACCCCTCCATGATGGAACAG TATGACCTCTCCAGTCTAGTGAAGTCTGAAGGTGGCTCTGGAGGAAACTGGTATGCTATGGAAAACTCCC GGGAACATGTTACACGGAGGAAATACTACCTCAATGTGTGTCGGCCTCTGAATCCTGTGCCAGGCTGCGA TCGATATGCATCTGCTTGCCAGATGAAATATGAAAATCATGAGGGCTCCTTGGCAGAGACTGTATCCATC AGTAACTTGGGAGTTGCAAAGATAGGCCCTGTGGTGGAGGAGAGTGGCAGCCTCCTCTTAGAGTATGTGA ACGGCTCTGCTTGCACCACCAGTGATGGCCAGCTCACCACCTACAGCACCAGGATCCATCTTGTCTGTGG TCGAGGATTTATGAACTCCCATCCCATATTCACTTTCAACTGGGAATGTGTGGTCAGTTTCTTGTGGAAC ACGGAGGCTGCCTGCCCTATCCAGACAATCACAGAAACAGACCAGGCTTGCTCAATAAGGGACCCCAGCA GTGGGTTTGTGTTTAATCTTAGCCCACTCAACGATTCTGCTCAAGGACATGTGGTCTTAGGCATCGGAAA GACTTTTGTGTTCAATATCTGTGGTGCAATGCCTGCCTGTGGAACGGTTGCGGGGAAGCCAGCCTATGGC TGCGAGGCCGAAACTCAGATAGAAGATATTAAGGACCTAAGGCCACAAAGGCCGGTCGGGATGGAGAGGA GCCTCCAGTTGTCTGCCGAGGGATTCCTCACTCTGACCTACAAGGGCTCTTCTCCCTCTGACAGAGGCAC AGCATTCATCATCCGTTTTATTTGCAACGATGACATTTACCCAGGAGCCCCCAAATTCCTGCATCAGGAC ATTGACTCCACACGAGGGATCCGGAACACTTACTTTGAGTTTGAAACTGCATTGGCTTGTACTCCTTCTC TAGTGGATTGTCAAGTGACTGACCCAGCTGGGAACGAGTATGACTTGAGCGCCCTGAGCATGGTCAGGAA GCCATGGACTGCCGTGGACACGTCTGCATACGGGAAGAGGAGGCACTTCTACCTGAGTGTCTGCAACCCT CTGCCTTATATCCCTGGCTGCCACGGCATTGCATTGGGGTCCTGCATGGTGTCAGAAGACAACAGTTTTA ACTTGGGTGTGGTGCAGATCAGTCCTCAAGCTACTGGAAATGGGTCTCTCAGCATCCTGTACGTGAATGG GGACAGATGTGGGGACCAGCGCTTCTCCACCAGGATCGTGTTCGAGTGTGCCCAGACGTCGGGCTCACCT ATGTTCCAGTTCGTGAACAACTGTGAGTATGTGTTTGTGTGGCGAACAGTGGAGGCCTGCCCTGTGATCA GAGAGGAAGGAGACAACTGCCAGGTGAAGGACCCCAGGCATGGCAATTTGTATGACCTGAAGCCCCTGGG CCTCAATGACACCATCGTCAGTGTTGGCGAGTACACCTACTACTTGCGGGTCTGCGGGAAGCTGTCATCA GATGTCTGCTCTGCCCATGACGGATCCAAGGCAGTCTCATCATGCCAGGAAAAGAAAGGACCACAGGGAT TCCAGAAAGTGGCAGGACTCCTTAGCCAGAAGCTGACTTTCGAAAACGGACTGTTGAAAATGAACTATAC TGGGGGGGATACCTGCCATAAGGTGTATCAGCGCTCCACAACCATCTATTTCTACTGTGACCGGACTACA CAGAAGCCAGTGTTTCTGAAGGAAACTTCGGACTGCTCCTATATGTTTGAGTGGCGAACACAGTATGCCT GCCCACCTTTCAATGTGACAGAGTGTTCTGTCCAGGATGCGGCTGGCAACTCCATTGACCTCTCCTCACT GTCAAGGTACAGTGACAACTGGGAGGCTGTGACCAGGACAGGGGCCACTGAGCATTACCTCATCAATGTA TGCAAGTCTCTGTCTCCACACGCTGGCACAGAGCCATGCCCTCCAGAGGCAGCTGTTTGTCTGCTGAATG GCTCTAAGCCTGTGAACCTTGGCAAGGTGAGGGATGGCCCTCAGTGGACAGACGGTGTGACTGTCCTGCA GTATGTGGATGGTGACTTGTGCCCAGACAAAATTCGGAGAAGGTCAACCATTATTCGCTTCACCTGCAGT GACAACCAAGTGAACTCCAGGCCCCTTTTCATCAGTGCTGTTCAGGACTGTGAGTACACCTTCTCCTGGC CCACCCCTTCTGCCTGTCCTGTGAAGAGCAACACACATGATGACTGCCAAGTCACCAACCCCAGCACAGG CCACCTGTTTGACCTGAGCTCCTTAAGTGGCAGAGCTGGTATCAATGCCTCCTACAGCGAGAAGGGGCTG GTCTTCATGAGCATCTGTGAGGAGAATGAGAACTGTGGCCCCGGAGTAGGGGCCTGCTTTGGACAGACGA GGATCAGTGTGGGCAAGGCCAGCAAGAGGCTGAGCTACAAGGACCAAGTCCTGCAGCTGGTGTATGAGAA TGGGTCCCCATGCCCTTCCCTGTCAGACCTGAGGTACAAGAGTGTCATCAGCTTTGTGTGCCGGCCTGAG GCTGGACCTACCAACAGGCCGATGCTCATCTCCTTGGACAAGCAGACGTGCACACTCTTCTTCTCCTGGC ACACACCCCTGGCCTGTGAGCAAGCGACGGAGTGTACTGTGCGGAATGGAAGCTCGATTATTGACCTGTC CCCTCTCATCCACCGCACTGGTGGCTACGAAGCATACGATGAAAGTGAGGATGACACCTCTGACACCACC CCTGACTTCTATATCAACATCTGCCAGCCACTGAATCCCATGCACGGAGTGCCCTGCCCTGCAGGTGCCT CAGTATGCAAAGTTCCTGTGGACGGGCCCCCTATAGATATTGGCAGAGTCACAGGCCCTCCAATATTCAA TCCTGTGGCGAATGAAGTCTACCTGAACTTTGAGAGCAGCACTCATTGCTTAGCCGACAGATATATGAAC TACACCTCACTCATCACATTTCACTGCAAGAGAGGAGTCAGCATGGGAACTCCTAAGCTGATAAGGACCA ATGACTGCGACTTTGTGTTTGAGTGGGAGACTCCGATTGTCTGTCCTGACGAAGTGAAGACACAGGGTTG TGCTGTAACAGATGAGCAGCTGCTCTATAGCTTCAACCTGACCAGCCTTTCCACAAGCACTTTCAAGGTT ACTCGGGATGCCCGCACATACAGTATAGGGGTGTGCACAGCAGCAGCTGGCTTAGGCCAGGAAGGCTGCA AGGATGGCGGTGTCTGTCTGCTCTCTGGGAACAAAGGAGCTTCTTTTGGGCGCCTTGCATCGATGCAGCT GGATTACAGGCACCAAGATGAAGCAGTCATTCTGAGTTATGTGAATGGTGATCCTTGCCCTCCAGAAACG GATGATGGCGAGCCCTGTGTCTTCCCCTTCATTTACAAAGGGAAGAGCTATGATGAATGTGTATTAGAGG GACGAGCGAAACTGTGGTGTAGCAAAACTGCAAATTATGATCGTGACCACGAGTGGGGCTTCTGCAGACA GACCAACAGCTACCGGATGTCTGCGATCATATTTACGTGTGATGAAAGTGAAGATATTGGGAGACCACAA GTGTTCAGTGAAGATCGTGGGTGTGAGGTGACATTTGAGTGGAAGACAAAAGTTGTCTGCCCTCCAAAGA AGATGGAGTGCAAGTTTGTTCAGAAGCATAAGACCTATGACTTGCGACTGCTCTCATCCCTCACGGGGTC CTGGGACTTTGTGCATGAAGGAAACTCGTACTTTATCAATCTGTGCCAAAGAGTATACAAAGGTCCCCTG GACTGCTCTGAAAGAGCCAGCATTTGCAAGAAGAGTGCCACTGGTCAAGTCCAGGTTTTGGGGCTTGTTC ATACACAAAAATTGGAAGTCATAGATGAAACGGTCATTGTTACTTACTCGAAAGGCTATCCTTGTGGTGG GAATAAGACTGCATCGTCTGTAATCGAATTGACCTGTGCGAAGACTGTGGGCAGGCCTGCATTCAAGAGG TTTGACAGTGTCAGCTGCACCTACTACTTTTACTGGTACTCACGGGCTGCCTGCGCGGTGAGGCCTCAGG AGGTGACCATGGTGAACGGAACGCTCACCAACCCTGTGACCGGCAAGAGCTTCAGCCTCGGCGAGATTTA TTTTAAGCTCTTCAGCGCCTCTGGAGACATGAGGACCAATGGGGACAACTACCTGTATGAAATCCAGCTC TCCTCCATCACCAGCTCTTCTTACCCTGCATGCGCTGGTGCCAACATCTGCCAGGTGAAGCCCAATGACC AGCATTTCAGCAGGAAAGTAGGCACCTCTGACATGACCAAGTACTACGTCCAAGATGGAGATCTGGATGT GGTGTTTACCTCTTCCTCCAAGTGTGGGAAAGATAAGACCAAGTCTGTTTCTTCCACCATCTTCTTCCAC TGTGACCCTCTGGTGAAGGATGGGATCCCAGAGTTCAGCCACGAGACCGCTGACTGCCAGTACCTGTTCT CCTGGTACACTTCAGCTGTGTGTCCTCTGGGTGTGGACTTTGAGGATGAGAGCGCAGGGCCAGAGTATAA AGGACTCTCAGAGCGGAGTCAGGCTGTTGGGGCAGTCCTCAGTCTTCTCCTGGTGGCGCTGACTGGCTGC TTGCTGGCCTTACTGCTGCATAAGAAGGAGAGAAGGGAAACTGTGATAAACAAGCTGACCAGCTGCTGCA GAAGAAGCTCGGGCGTGTCCTACAAGTACTCAAAGGTCAGCAAGGAGGAGGAGACAGATGAGAATGAGAC AGAATGGCTGATGGAAGAGATCCAGGTGCCAGCCCCAAGACTAGGCAAGGACGGGCAAGAAAATGGCCAT ATCACCACCAAGGCGGTGAAAGCAGAAGCTCTCTCGTCCCTGCATGGGGACGACCAGGACAGTGAGGATG AGGTCCTGACCGTTCCAGAGGTGAAAGTTCACTCAGGCCGAGGAGCGGAGGTGGAAAGCTCACAGCCCCT GAGAAACCCACAGCGCAAGGTCCTGAAGGAAAGGGAGGGTGAGAGGCTGGGGCTGGTCCGAGGTGAGAAG GCCAGAAAAGGGAAGTTCAGACCCGGACAGCGCAAGCCAACAGCCCCTGCCAAGCTGGTGTCCTTCCATG ATGACAGCGACGAAGACCTCTTACACATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010515
Insert Size	7452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010515.2, NP_034645.2
RefSeq Size	8948 bp
RefSeq ORF	7452 bp
Locus ID	16004
UniProt ID	Q07113
Gene Summary	Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.[UniProtKB/Swiss-Prot Function]

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