Cenpe (NM_173762) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	312kDa; AU019344; BC049989; C530022J18; CENP-E; Kif10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225329 representing NM_173762 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGAGGCTTCAGTGGCTGTCTGTGTTCGTGTGCGACCCCTCAACAGCAGAGAAGAAGAACTTG GAGAAGCCACCCACATTTACTGGAAAACAGATAAAAACGCTATTTATCAGAGCGATGGGGGTAAATCTTT CCAATTTGATCGTGTCTTTGACAGTAATGAAACCACGAAGAATGTGTATGAAGAGATTGCGGTGCCCATC ATCAGCTCCGCCATACAAGGCTACAATGGTACTATATTTGCCTATGGACAAACTGCATCAGGAAAGACAC ACACGATGATGGGTTCTGAAGATTGTTTGGGAGTTATACCCAGGGCAATTCATGACATTTTCCAAAGGAT AAAGAAGTTTCCTGAGAGAGAATTTCTCCTACGAGTTTCTTACATGGAGATCTACAATGAAACCATTACA GATTTACTCTGTAATGCTCAAAAAATGAAACCTTTGATAATTCGGGAAGACACCAATAGGACTGTATATG TTTCTGATCTCACAGAAGAAGTGGTTTATACAGCAGAAATGGCTTTGAAATGGCTCGCAACAGGAGAAAA AAACAGACATTATGGAATAACCAAAATGAATCAAAGAAGCAGTCGTTCTCATACGATTTTTAGGATGATT CTGGAAAGTAGAGAAAAGGCTGAGCCTTCTAACTGCGATGGCTCCGTCAAAGTGTCTCATCTGAATTTGG TCGATCTTGCAGGCAGTGAAAGAGCTGCTCAAACAGGAGCTGAAGGTGTGCGATTGAAGGAGGGCTGTTT TATAAATCGGAACTTGTTTATTTTGGGACAAGTGATTAAGAAACTCAGTGATGGACAGGTTGGGGGTTTC ATAAATTACCGAGATAGCAAATTGACACGAATTCTTCAGAATTCCTTGGGAGGAAATGCCAAGACGCGCA TCATCTGCACGATCACCCCAGCGTCCCTGGACGAAACCCTGACGACGCTGCAGTTTGCCAGCACTGCTAA GTATATGAAGAATACTCCTTACGTTAATGAAGTGTCGAATGATGAAGCTCTCTTGAAAAGATACAGAAGA GAAATAGCGGATCTTAGAAAACAATTAGAGGAGGTAAATACAAAGACTCGGGCACAGGAAATGGAAAAGG ATCAACTGGCCCAGCTTCTGGATGAAAAAGACTTGCTTCAGAAAGTTCAGGATGAGAAAATTAATAACCT GAAGCGGATGCTGGTGACGTCATCTTCTATTGCATTGCAGCAGGAGCTAGAGATTAAAAGGAAACGAAGA GTTACTTGGTGCTATGGCAAAATGAAGGATTCAAACTATGAAAAGGAATTTAAAGTCCCGACAAGCATAA CAACAAGAAAACGTAAAACTTCTGTGACTTCTTTGAGAGAAAATTCTCTCATGAAGTTCGGAGAGAGTGC AGCCTCTTCCGAGTTTGAAATGTTGAATAACACTCTTGAGTCATTGGCTGAGGTAGAGTGGAGCTCGGCA ACAACGCTCCTAAGCGAGGAAAATGTAGAAAGTGAGTTGAACTCACTTAATGCTCAATATAATGATCTGG TATTAGACTATGAACAGCTCAGAAGAGAAAATGAAGACTTGAAATTGAAATTAAAAGAAAAAAATGAGTT AGAGGAATTTGAGCTTCTAGAACAAAAAGAAGAAAGAGATCAAGAGATGCAACTAATGCATGAAGTTTCC AACTTAAAGAATTTAATTAAACACGCAGAAGAATATAATCAAGACCTTGAGAATGACCTAAGTTCAAAAG TGAAGCTTCTTAAAGAAAAAGAGGAGCAGATTAAGAACCTGCAGGAATACATAGATGCCCAGAAGTCAGA AAAGATGAAGATAGACCTGTCATACACATCGGACGCCACTGAAGACCTGAAACAGGCGATGCGCACCCTG TCTGATTTGGACACAGTGGCCCTTGACGCTAAGAAAGAGTCAGCCTTCCTTAGAAGTGAAAATCTGGAGC TGAAGGAGAAAATTAATGAACTTTCGGATTCCCGTAAGCAAATGGAAAGTGACATTCAGATGTATCAACG CCAACTGGAAGCAAAAAAGAAAATGCAAACTGATCTGGATAAGGAATTACAGTTGGCTTTTCAAGAAATA TCAAAACTCAGCGCCCTGGTAGATGGCAAAGGTTTACTGTCTAATTTGGAATTGGAGAAAAGGATTACTG ATCTCCAAAAAGAACTAAATAAAGAAGCTGAAGAAAAACAAACTTTGCAAGAAGAAGTTAACTTGCTGTC GGAGTTGAAATCTTTACCGTCTGAGGTGGAAACGCTGAGGAGAGAGCTGTATGAGAAATCTGAAGAGCTC CATATAATAACCACAGAAAGAGAGAAACTGTTTTCTGAAATGGCTCATAAAGACAGTAGGATTCAAGGTT TACTTGAGGAAATTGGAAACACTAGAGATGACCTTGCCACTTCCCAGCTGAGTCGGAGGGGCAGTGATGG AGAATGGCAAGCCCTGGAAAGCCTGCACGCAGAGCTGGAGCACAGACACGCGGGGGTCCTGGAGGAGAGA GAGAGGCTGAAGCAGGAGATTGGGGCTCTCTCCAAAGAAGCAGAAAGCCTGGCTTTCAGTTTGGATTCCG TGAAGGCCGAGCTTTCTCACAAGACTCAAGAGCTTGAGCAGAAAACAGTTGAGGGTCAAGAAAGGCTAAA TAAAATGGAGGCGCTGAGAGAAGAGTTAGAAAGCAGAGACTCCAGCCTGCAGTCTGTAGAGAAGGAGAAA GTCCTGCTCACTGAGAAGCTGCAGCAAGCCTTGAAGGAAGTAAAAGCCTTAACCCAAGAAAAGAAAAACC TGAAGCAGCTCCAGGAGAGCCTGCAAACTGAGAGAGACCAACTCAGAAGTGACATTCAGGACACTGTGAA CATGAACATAGATACTCAAGAGCAGTTACTAAATGCTCTGGAGTCTCTGAAACAACACCAAGAAACAATT AACATGCTGAAAATGAAAGCTGCTGAGGAACTATCCGATAATTTGCATGTAAAGGACAGGGGAGGAGCTA GAGATGAAGCTCAGCAAAAGATGGATGGCATAGATGAACAGAATGAGAGTGCCCACACATTGCTTGGTGG TGGTAAGGACAATGAGGTAACTGAGGAACAGAGGAAAATCGACTCTCTAATGCAGGAGAATAGCGGACTC CAACAGACACTGGAAAGTGTTAGAGCAGAAAAGGAACAACTGAAGATGGACCTAAAGGAAAATATCGAAA TGAGTATTGAAAACCAGGAAGAGTTAAGAATTCTTAGAGATGAACTGAAAAGACAACAAGAGGTAGCTGC ACAGGAAAAGGATCATGCCACCGAGAAGACCCAGGAGCTCTCCAGGACGCAGGAAAGATTGGCAAAAACA GAAGAGAAGTTAGAGGAAAAGAACCAGAAACTCCAAGAGACACAGCAGCAACTTCTTAGTACACAAGAAG CTATGAGTAAGCTGCAGGCAAAGGTGATTGACATGGAAAGCTTACAGAATGAATTCAGGAACCAAGGATT GGCATTGGAGCGTGTGGAGACAGAGAAACTGGAGTTAGCTCAGAGACTTCATGAAAGTTATGAGGAGGTG AAATCTATAACCAAAGAAAGAAATGATCTAAAGGAATTGCAGGAGTCATTTGAAATAGAGAAGAAGCAAC TTAAAGAATATGCAAGGGAAATAGAAGCCGCGGGCCTACAAACAAAAGAAGAACTAAACATTGCCCACGC GAATCTAAAAGAGTACCAGGAAATCATCACAGAGCTAAGAGGAAGCATTTCTGAAAACGAAGCACAAGGA GCAAGCACCCAGGACACGGCAAAAAGCGCCCCTGAGCTACAAGGAGAGGTTCCAGAGCAAGAGCTGCTTC CTGTTGTCAAAGAAGCCAGGCACAGTGCAGAAAAAGTGAACGGACTGGAGCCAGTGGGAGCACATTCCAG AACAGTGCACTCCATGACAATGGAGGGCATAGAAATAGAAAACCTCAGGCTGACCAAGAAACTTGAAGAA AGTCAGATGGAGATCAGCTGTCTCACTAGAGAGAGAGAAGACCTGAGAAGGACACAGGAAACACTTCAGG TCGAATGTACCCAGCTGAAAGAAGATGCCAGAAGAACTTTGGCTAATCATCTAGAAACTGAAGAGGAACT TAACCTTGCTCGTTGTTGCCTGAAAGAGCAGGAGAACAAGATTGACACCCTAATAACGAGCCTCTCGCAG AGGGAAACTGAGCTGTCCAGTGTGCGAGGACAACTAGCGCTGACTACTGCTGAACTGGAGAGAAAGGTTC AAGAACTCTGTGAGAAACAGGAAGAACTTACTAGGAAAGAAACTAGCGAGGCTCAAGGGAAGATGAGTGA ACTAGAGCAACTCAGGGAGCTGCTGCTTGCCCAGGCCTCGGCACTGCAGAACGCAGAGAGTGACAGGCTG CGGCTGAATACACAACTGGAAGAAAGTCAAGAGGAAATGAAAACTCTAAGGGAGGAAAGAGAAGAGCTGA GAAGAATGCAAGAGGCTCTTCACGTGGAAAGTGAGCAGCAGAAAGAAAGCATGAAGGAAATCAGCTCAAA ACTCCAAGAACTACAGAACAAAGAGTATGAATGTCTTGCAATGAAGACTATTAATGAGACTCAAGGAAGC AGATGTGAAATGGACCACTTGAACCAGCAGCTGGAGGCCCAGAAGTCAACTCTGGAAAAGGTGGAGATGG AGAATGTAAACTTGACTCAAAGACTTCATGAGACCCTTGAGGAAATGAGATCTGTAGCAAAAGAAAGAGA TGAGCTTTGGAGCATGGAGGAGCGCCTCACAGTAGAGAGAGACCAACTGAAGAAGAGCCTTGAAGAAACA GTAACTAAAGGTATGGAAAAAGAGGAAGAGCTAAGAGTTGCGCATGTGCATCTGGAAGAGCACCAGGAAA CTATCAACAAGCTCAGAAAGATGGTGTCTGACTACACAGATGAAATATCACATACTCAAGGGGACCTAAA GCACACAAATGCCGTTGTAGAAGCTCAGAACCAAGATCTCCGGGAAAAAGAACATCAACTTTCTCAAGTA AAAGCTGATCTCAGAGAAACCGTGGATCAAATGGAGCAACTGAAGAAGAAGCTCGAAGCCCAGAGTTCGA CTCTAGAAAGCAGAGAAATAGAGAAGTTAGAATTGACTCAGCAACTTAACGAAAACCTTAAAAAAATAAC ACTTGTCACTAAGGAAAATGATAGCCTAAAAATTATGGATGAAGCCCTCAGGGAAGAACGAGACCAGCTA CGGAAAAGTCTTCAGCAAACAGAGGCAAGGGATCTGGAAAATCAAGAGAAACTAAGAATTGCTCACATGA ATCTAAAAGAGCACCAAGAAACTATTGACCGACTCATGGAGACTATGTCTGAGAAGACAGAGGAAATATC AAATATGAAAATGGAGTTAGAAAATGTAAACATGAAATTACAAGAAAAGGTTCAAGAACTTAAGACAAGT GAGCGTCAACGTGTTAAGTTAAAAGCAGATGCCAGTGAAGCCAAGAAGGAGCTCAAGGAGCAGGGCTTAA CTCTGAGTAAGATAGAGATGGAGAACTTAAATCTCGCTCAGAAGATTCATGAGAACCTTGAAGAAATGAA ATCTGTAAGAAAAGAAAGAGATGATCTAAAGAAACTTGAGGAGATACTCAGAATGGAGAGAGACCAGCTC AAGGACAACCTGAGAGAAGCCATGCTTAAGGCCCACCAGAACCATGAAGAGACAATGAAATGTGGAAAGG GGTTACTGTGTGCTGGAGAATACTGTACAGGAAGACTTCGAGAAAAGTGCTTCAGGATAGAAAAACTCCT GAAAAGATACTCAGAAATGGCTAATGATTATGAGTGCTTGAATAAAGTCTCTCTTGACTTGGAGAGAGAA ACTAAAACCCAAAAAGAGCTGTCAGTTACTGTAAGAACAAAGCTCTCACTTCCGCACACACAGACCAAAG AGATGGAAAAGCTTCTGACTGCAAACCAGAGATGTTCCCTGGAGTTCCACAGAGCCCTCAAGAGACTTAA GTACGTGTTAAGCTCCATTGCGAGGATAAAGGAAGAGCAGCATGAGTCCATCAATAAACGTGAAATGGCT TTTATTCAGGAAGTGGAAAAGCAGAATGAACTGCAGATTCAAATCCAGAGCCTTTCACAGACTTACCGTA TTCCAGCCAGAGACTTACAGATCAAACTGAGCCAGGAGATGGACCTCCATATTGAGGAAATGCTCAAAGA TTTTTCAGAAAATGATTTCCTCACCATAAAAACTGAAGTCCAACAAGTATTAAATAATAGGAAAGAAATA ACTGAGTTTTTGGGAAAATGGCTAAATACTCTTTTTGATACAGAAAATCTTAAGAGCACCATCCAGAAAG AAAACAAAAGCATTGGGCTCGTGAATAACTTCTATCACAGCAGAATCACTGCCATGATAAATGAATCAAC GGAGTTTGAGGAAAGAAGTGCTACCAGATCCAAAGACTTGGATCAGTACCTGAAGTCACTGAAAGAAACA ACTGAGCAACTGTCTGAAGTCTACCAGACACTGACCGCCTCCCAGTCTGTTGTGCATCTCCATCCTACCG TGCAGCCTTCCACACGGGACAGTGAGCGTCCTCAGGCTGCATCAGGAGCTGAACAACTGACCTCAAAGAA CAAAATTGCTTTAGGAGCTGTGCCCTATAAAGAAGAAATTGAGGATCTCAAAATGCAGCTGGTGAAGAGT GACCTAGAGAAGAAGGCGACCGCCAAGGAATTCGATAAGAAAATTCTCTCTCTAAAAGCCACTGTTGAAC ATCAAGAAGAAATGATAAGGCTACTGAGAGAAAATCTTAGAGGACATCAACAGGCCCAGGATACCTCAAT GATCTCAGAACAAGACTCTCAGCTTCTGAGTAAACCTCTAACCTGTGGAGGTGGCAGTGGAATTGTACAA AGCACAAAAGCTCTTATTTTGAAAAGTGAATATAAAAGGATGGGAAGTGAGATTTCTAAGTTAAAGCAGC AAAATGAGCAGCTAAGAAAGCAAAATAATCAACTATTAAGTGATAACTCTCAACTCTCCAATGAGGTTAA AACCTGGGAGGAAAGAACTCTTAAAAGAGACAGTTACAGAGAAACAACTTGTGAGAATTCTCCAAAGTCT CCTAAAGTGACTCGGACAGATTCTAAAAGGAGACAGAACACAACTTCTCAGTGCAGGGCACAAAATCTCC AGGATCCTGTGCCAAAAGATTCCCCAAAATCATGGTTTTTTGATAACCGGTCAAAGTCTCTTCCAGCACC TCACCCGATCCGCTATTTTGACAACTCCAGTCTAGGGCTGTGCCCAGAACCAGATGACGTAGAGAATGTG GAGCCTAAGACAGACCTCTGCCAAGCCTCCTTGGAAAAAGATGTGTCCCAGTGCAAGACGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_173762
Insert Size	7416 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_173762.4, NP_776123.3
RefSeq Size	7813 bp
RefSeq ORF	7416 bp
Locus ID	229841
UniProt ID	Q6RT24
Gene Summary	Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (By similarity). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:12925705). The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC (PubMed:12361599). Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (PubMed:12925705).[UniProtKB/Swiss-Prot Function]

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