Ep300 (NM_177821) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430090G16; A730011L11; KAT3B; p300; p300 HAT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225306 representing NM_177821 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGAATGTGGTGGAACCCGGGCCGCCTTCAGCCAAGCGGCCTAAACTCTCATCTCCGGCCCTCT CGGCGTCCGCCAGCGATGGCACAGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATT AATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCAGACAAGTCTTGGCATA GTACAAGATGCAGCCTCGAAACATAAACAGCTGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCA ACATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTT GATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGT AGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCA TGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGAT AATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAAT GCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGAC AGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACC ATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTT CTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGG GCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGG AGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCAC AAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGA AAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTC TCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAAT GCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTC TAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCG AGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAG AATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTC CTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAAC TATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCA GCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACC TTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGA AAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATAC TATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTAC AGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGG GCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCG AACCACATGATGCCTCGGATGACTCCACAGCCTGGTTTGAATCAATTTGGGCAGATGAATATGCCACAGC CCCCTATTGGACCCCGGCAACCCTCTCCTCTTCAGCACCATGGACAATTGGCTCAGTCTGGGTCACTCAA TCCGCCTATGGGCTATGGACCTCGCATGCAGCAGGCTTCCGGCCAGAACCAGTTCCTCTCCCAGACTCAG TTCACATCCCAAGGAATGAATGTAACAAACATGCCTTTGGCTCCATCCAGCGGTCAAGCACCAGTGTCTC AAGCACAAATGTCCAGTTCTTCTTGCCCAGTGAACTCTCCTATAATGCCTCCAGGGTCTCAAGGGAGCCA CATTCACTGTCCTACTCTGCCTCAACAAGCTCATCAGAATTCACCTTCTCCTGTTCCTAGCCGCACCCCT ACTCCTCATCATACCCCCCCAAGCATAGGGAATCAACCACCACCAGCAACAGCAATTCCAACACCTGTTC CTACACCTCCTGCCATACCACCTGGACCACAGCCCCCATCTCTGCATCCCTCATCTAGACAAACACCTAC ACCACCAACACATCTGCCTCCCCAAGTGCAGCCTTCACTTCCTGCTGCTCCTTCTGCTGACCAGTCCCAG CAACAGCCTCGGTCACAGCAGAGCACAGCAGTTTCTGTTCCTACCCCAACAGCACCATTGCTGCCTCCTC AGCCTTCCACTCCGCTTTCTCAGCCAGCTGTGAGCATTGAAGGCCAGGTCTCAAACCCTCCATCTACTAG TAGCACCGAAGTGAATTCTCAGACCATTCCTGAGAAGCAGCCTTCACAGGAAGTGAAAATGGAGTCTAAA ATGGAGGTGGATAAGCCAGAACCAGCAGATGCTCAACCTGAGGATACAAAGGAGGCTAAAGGTGAGGATG TTAAAGTAGAACCTACAGAAATGGAGGAGAGAGGCCCTGAGTTAAAAACTGATGGGAAAGAGGAGGAAGA ACAGCCAAGCACCTCTGCTACCCAGTCCTCCCCAGCTCCTGGACAGTCAAAGAAGAAGATTTTCAAACCA GAGGAATTACGACAGGCTCTGATGCCAACATTGGAGGCACTTTACCGGCAGGACCCAGAATCTCTTCCCT TTCGTCAGCCTGTGGACCCGCAGCTTCTAGGAATCCCTGATTACTTTGATATTGTGAAGAGCCCCATGGA TCTTTCTACAATCAAGAGGAAGTTAGATACTGGACAATACCAAGAGCCCTGGCAATATATAGATGATATT TGGCTTATGTTCAACAATGCCTGGTTATATAACCGTAAAACTTCAAGGGTATACAAATATTGCTCTAAAC TTTCTGAAGTATTTGAACAAGAAATTGACCCTGTCATGCAAAGCCTTGGATACTGTTGTGGCAGAAAGTT GGAGTTTTCTCCACAGACTCTTTGTTGCTATGGGAAACAGTTATGCACAATCCCCCGTGATGCTACTTAC TACAGTTACCAGAACAGGTATCATTTCTGTGAGAAGTGTTTCAATGAAATCCAAGGGGAGAGCGTTTCTT TGGGTGATGACCCTTCCCAACCTCAAACTACAATAAATAAAGAACAGTTTTCCAAGAGAAAAAATGACAC GTTGGATCCTGAACTGTTTGTTGAGTGCACAGAGTGTGGAAGAAAGATGCACCAGATCTGTGTCCTTCAC CACGAGATCATCTGGCCATCTGGGTTTGTCTGTGATGGCTGTTTAAAGAAAACTGCACGAACTAGGAAAG AAAATAAGCTTTCTGCTAAAAGATTGCCATCTACCAGACTTGGGACCTTTCTGGAGAATCGAGTGAATGA CTTTCTGAGGCGACAAAATCACCCTGAATCAGGAGAGGTCACTGTTCGGGTTGTTCATGCTTCTGACAAA ACTGTGGAAGTGAAACCAGGCATGAAAGCAAGGTTTGTAGATAGTGGAGAGATGGCAGAATCTTTTCCAT ACCGAACAAAGGCCCTGTTTGCCTTTGAAGAAATTGATGGTGTTGACTTGTGTTTCTTCGGCATGCATGT TCAAGAATATGGCTCTGACTGCCCCCCTCCCAACCAGAGGAGGGTATACATATCTTACCTCGATAGTGTT CATTTCTTCCGTCCTAAATGCTTGCGGACTGCAGTCTATCATGAAATTCTAATTGGATATTTGGAATATG TCAAGAAATTAGGATACACAACAGGGCACATCTGGGCCTGTCCACCCAGTGAAGGGGATGACTATATCTT CCATTGCCATCCACCTGATCAGAAGATACCAAAGCCCAAGCGCCTGCAAGAATGGTACAAAAAGATGCTT GACAAGGCTGTATCAGAACGTATTGTCCATGACTACAAGGATATTTTAAAACAAGCTACTGAAGATCGAT TAACAAGTGCAAAGGAACTACCCTACTTTGAAGGTGATTTCTGGCCCAATGTTCTAGAAGAAAGCATCAA GGAGCTTGAACAGGAAGAGGAAGAGAGGAAACGGGAAGAGAACACCAGCAACGAGAGTACTGATGTAACA AAAGGAGACAGCAAAAATGCTAAGAAGAAGAATAACAAGAAAACCAGCAAAAACAAGAGCAGCCTGAGTA GGGGCAATAAGAAGAAGCCTGGTATGCCCAATGTGTCTAATGACCTTTCTCAGAAACTGTATGCCACCAT GGAAAAGCATAAAGAGGTTTTCTTTGTGATCCGCCTCATCGCTTGTCCTGCTCCCAATTCCCTGCCTCCC ATTGTTGATCCTGACCCTCTCATCCCCTGTGACCTGATGGACGGTAGAGATGCATTTCTCACCCTTGCAA GGGACAAGCACCTGGAGTTCTCTTCACTTCGAAGAGCCCAGTGGTCTACCATGTGCATGCTGGTGGAGCT CCATACACAGAGTCAGGATCGCTTTGTCTACACCTGCAACGAGTGCAAGCACCACGTGGAGACACGCTGG CATTGCACTGTGTGTGAGGATTATGACCTGTGCATCACTTGTTATAACACTAAAAATCATGACCACAAAA TGGAGAAACTAGGCCTTGGCTTAGATGATGAGAGCAATAACCAGCAAGCTGCAGCTACACAGAGCCCAGG AGACTCCCGCCGCCTGAGCATCCAACGCTGCATCCAGTCTCTGGTGCATGCCTGTCAGTGCCGCAACGCC AACTGCTCCCTGCCTTCCTGCCAGAAGATGAAGAGGGTTGTGCAGCACACCAAAGGCTGCAAACGGAAAA CCAATGGTGGGTGCCCCATTTGCAAACAGCTTATTGCCCTCTGCTGCTATCATGCCAAGCACTGCCAGGA GAACAAGTGCCCGGTGCCATTTTGCCTCAACATCAAGCAAAAGCTCCGGCAGCAACAGCTGCAGCACCGG CTCCAACAGGCTCAGATGCTCCGTAGGAGGATGGCCAGCATGCAACGAACTGGTGTGGCGGGGCAGCAGC AGGGCCTGCCGTCCCCAACTCCTGCTACACCAACTACCCCTACTGGCCAACAGCCAGCCACCCCACAGAC ACCCCAGCCCCAACCCACCTCTCAACCACAGCCCACTCCTCCCAACAACATGACACCCTACTTACCCAGG ACTCAGACTACTGGCCCTGTGTCCCAGGGTAAGGCACCAGGCCAGGTGACCCCACCTACCCCACCTCAGA CTGCTCAGGCCCCCCTTCCAGGGCCTCCACCTGCAGCAGTAGAAATGGCAATGCAAATTCAGAGGGCAGC AGAGACACAGCGCCAGATGGCCCATGTACAGATTTTCCAAAGGCCAATCCAGCACCAGATGCCCCAGATG TCACCCATGGCCCCTATGGGCATGAACCCACCTCCTATGGCCAGAGGTCCTGGTGGGCATTTGGATCCAG GAATAGGTCCAGCAGGAATGCAGCAACAGCCACCTTGGGCCCAAGGAGGAATGCCTCAGCCCCAGCAGAT GCAGTCAGGGATGCCAAGGCCAGCCATGATGTCAGTGGCCCAGCATGGTCAGCCTTTAAACATGGCTCCA CAACCAGGATTGGGCCAAGTGGGTGTGAGCCCTCTCAAGCCAGGCACTGTGTCTCAACAAGCCTTACAAA ACCTTTTGCGGACTCTCAGGTCTCCCAGTTCTCCCTTACAGCAGCAACAGGTGCTTAGTATCCTTCATGC CAACCCCCAACTGTTGGCTGCATTCATCAAGCAGCGGGCTGCCAAGTATGCCAACCCTAATCCACAGCCT CTCCCTGGACAGCCTGGCATGACCCAGGGACAGCCAGGGCTGCAGCCACCTACCATGCCTGGCCAGCAAG GTGTCCACTCCAACCCAGCCTTGCAGAACATGAATCCTCTGCAGGCAGGTGTCCAGAGAGCTGGCCTGCC CCAGCAGCAGCCGCAGCAGCAGCTCCAGCCACCCATGGGAGCAATGAGTCCCCAAGCTCAGCAAATGAAC ATGAATCACAATACCATGCCTTCACAGTTCAGAGACATCTTAAGACGGCAGATGATGCAACAGCAGGGAG CAGGGCCAGGAATCGGCCCTGGAATGGCCAACCAGTTCCAGCAGCCCCAAGGAATTGGCTATCCACCGCA GCAGCAGCAGCAGCAGCGAATGCAGCATCACATGCAGCAAATGCAGCAAGGAAATATGGGACAAATGGGT CAGCTTCCCCAGGCTCTGGGGGCTGAGGCAGGAGCCAGTCTACAGGCCTATCAGCAGCGACTTCTTCAAC AACAGATGGGGTCTCCTGCTCAGCCCAACCCTATGAGCCCACAGCAGCACATGCTCCCAAATCAGGCACA GTCCCCACACCTACAAGGTCAACAAATCCCTAATTCTCTCTCCAATCAAGTGCGCTCTCCCCAGCCTGTC CCTTCTCCTCGACCACAGTCTCAGCCCCCCCATTCCAGCCCATCTCCGAGGATGCAGCCTCAGCCTTCTC CACACCATGTTTCTCCACAGACCAGTTCCCCACATCCTGGACTGGTAGCTGCCCAGGCTGCCAACCCTAT GGAACAAGGGCATTTTGCCAGCCCAGACCAGAATTCAATGCTTTCACAGCTCGCTAGCAATCCTGGCATG GCAAACCTCCACGGGGCAAGTGCCACGGACCTGGGACTGAGCAGCGATAATGCAGACTTGAATTCAAACC TCTCACAGAGTACACTAGACATACACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177821
Insert Size	7239 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177821.6, NP_808489.4
RefSeq Size	8749 bp
RefSeq ORF	7239 bp
Locus ID	328572
UniProt ID	B2RWS6
Gene Summary	Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (By similarity). Acetylates all four core histones in nucleosomes (By similarity). Histone acetylation gives an epigenetic tag for transcriptional activation (By similarity). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein (PubMed:18486321, PubMed:24216764). Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity). Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (By similarity). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2 (PubMed:28883095). Acetylates 'Lys-131' of ALX1 and acts as its coactivator (By similarity). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (By similarity). Acetylates HDAC1 leading to its inactivation and modulation of transcription (By similarity). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (By similarity). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium (By similarity). Promotes cardiac myocyte enlargement (By similarity). Can also mediate transcriptional repression (By similarity). Acetylates FOXO1 and enhances its transcriptional activity (By similarity). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (By similarity). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (By similarity). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:14645221, PubMed:9512516). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (By similarity). Acetylates MEF2D (By similarity). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:27105113). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (By similarity). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:27105113). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (By similarity). Acetylates PCK1 and promotes PCK1 anaplerotic activity (By similarity).[UniProtKB/Swiss-Prot Function]

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