Ep300 (NM_177821) Mouse Untagged Clone

CAT#: MC225306

Ep300 (untagged) - Mouse E1A binding protein p300 (Ep300), (10ug)



  "NM_177821" in other vectors (2)

CNY 38,760.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A430090G16; A730011L11; KAT3B; p300; p300 HAT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225306 representing NM_177821
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAATGTGGTGGAACCCGGGCCGCCTTCAGCCAAGCGGCCTAAACTCTCATCTCCGGCCCTCT
CGGCGTCCGCCAGCGATGGCACAGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATT
AATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCAGACAAGTCTTGGCATA
GTACAAGATGCAGCCTCGAAACATAAACAGCTGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCA
ACATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTT
GATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGT
AGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCA
TGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGAT
AATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAAT
GCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGAC
AGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACC
ATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTT
CTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGG
GCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGG
AGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCAC
AAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGA
AAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTC
TCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAAT
GCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTC
TAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCG
AGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAG
AATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTC
CTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAAC
TATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCA
GCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACC
TTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGA
AAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATAC
TATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTAC
AGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGG
GCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCG
AACCACATGATGCCTCGGATGACTCCACAGCCTGGTTTGAATCAATTTGGGCAGATGAATATGCCACAGC
CCCCTATTGGACCCCGGCAACCCTCTCCTCTTCAGCACCATGGACAATTGGCTCAGTCTGGGTCACTCAA
TCCGCCTATGGGCTATGGACCTCGCATGCAGCAGGCTTCCGGCCAGAACCAGTTCCTCTCCCAGACTCAG
TTCACATCCCAAGGAATGAATGTAACAAACATGCCTTTGGCTCCATCCAGCGGTCAAGCACCAGTGTCTC
AAGCACAAATGTCCAGTTCTTCTTGCCCAGTGAACTCTCCTATAATGCCTCCAGGGTCTCAAGGGAGCCA
CATTCACTGTCCTACTCTGCCTCAACAAGCTCATCAGAATTCACCTTCTCCTGTTCCTAGCCGCACCCCT
ACTCCTCATCATACCCCCCCAAGCATAGGGAATCAACCACCACCAGCAACAGCAATTCCAACACCTGTTC
CTACACCTCCTGCCATACCACCTGGACCACAGCCCCCATCTCTGCATCCCTCATCTAGACAAACACCTAC
ACCACCAACACATCTGCCTCCCCAAGTGCAGCCTTCACTTCCTGCTGCTCCTTCTGCTGACCAGTCCCAG
CAACAGCCTCGGTCACAGCAGAGCACAGCAGTTTCTGTTCCTACCCCAACAGCACCATTGCTGCCTCCTC
AGCCTTCCACTCCGCTTTCTCAGCCAGCTGTGAGCATTGAAGGCCAGGTCTCAAACCCTCCATCTACTAG
TAGCACCGAAGTGAATTCTCAGACCATTCCTGAGAAGCAGCCTTCACAGGAAGTGAAAATGGAGTCTAAA
ATGGAGGTGGATAAGCCAGAACCAGCAGATGCTCAACCTGAGGATACAAAGGAGGCTAAAGGTGAGGATG
TTAAAGTAGAACCTACAGAAATGGAGGAGAGAGGCCCTGAGTTAAAAACTGATGGGAAAGAGGAGGAAGA
ACAGCCAAGCACCTCTGCTACCCAGTCCTCCCCAGCTCCTGGACAGTCAAAGAAGAAGATTTTCAAACCA
GAGGAATTACGACAGGCTCTGATGCCAACATTGGAGGCACTTTACCGGCAGGACCCAGAATCTCTTCCCT
TTCGTCAGCCTGTGGACCCGCAGCTTCTAGGAATCCCTGATTACTTTGATATTGTGAAGAGCCCCATGGA
TCTTTCTACAATCAAGAGGAAGTTAGATACTGGACAATACCAAGAGCCCTGGCAATATATAGATGATATT
TGGCTTATGTTCAACAATGCCTGGTTATATAACCGTAAAACTTCAAGGGTATACAAATATTGCTCTAAAC
TTTCTGAAGTATTTGAACAAGAAATTGACCCTGTCATGCAAAGCCTTGGATACTGTTGTGGCAGAAAGTT
GGAGTTTTCTCCACAGACTCTTTGTTGCTATGGGAAACAGTTATGCACAATCCCCCGTGATGCTACTTAC
TACAGTTACCAGAACAGGTATCATTTCTGTGAGAAGTGTTTCAATGAAATCCAAGGGGAGAGCGTTTCTT
TGGGTGATGACCCTTCCCAACCTCAAACTACAATAAATAAAGAACAGTTTTCCAAGAGAAAAAATGACAC
GTTGGATCCTGAACTGTTTGTTGAGTGCACAGAGTGTGGAAGAAAGATGCACCAGATCTGTGTCCTTCAC
CACGAGATCATCTGGCCATCTGGGTTTGTCTGTGATGGCTGTTTAAAGAAAACTGCACGAACTAGGAAAG
AAAATAAGCTTTCTGCTAAAAGATTGCCATCTACCAGACTTGGGACCTTTCTGGAGAATCGAGTGAATGA
CTTTCTGAGGCGACAAAATCACCCTGAATCAGGAGAGGTCACTGTTCGGGTTGTTCATGCTTCTGACAAA
ACTGTGGAAGTGAAACCAGGCATGAAAGCAAGGTTTGTAGATAGTGGAGAGATGGCAGAATCTTTTCCAT
ACCGAACAAAGGCCCTGTTTGCCTTTGAAGAAATTGATGGTGTTGACTTGTGTTTCTTCGGCATGCATGT
TCAAGAATATGGCTCTGACTGCCCCCCTCCCAACCAGAGGAGGGTATACATATCTTACCTCGATAGTGTT
CATTTCTTCCGTCCTAAATGCTTGCGGACTGCAGTCTATCATGAAATTCTAATTGGATATTTGGAATATG
TCAAGAAATTAGGATACACAACAGGGCACATCTGGGCCTGTCCACCCAGTGAAGGGGATGACTATATCTT
CCATTGCCATCCACCTGATCAGAAGATACCAAAGCCCAAGCGCCTGCAAGAATGGTACAAAAAGATGCTT
GACAAGGCTGTATCAGAACGTATTGTCCATGACTACAAGGATATTTTAAAACAAGCTACTGAAGATCGAT
TAACAAGTGCAAAGGAACTACCCTACTTTGAAGGTGATTTCTGGCCCAATGTTCTAGAAGAAAGCATCAA
GGAGCTTGAACAGGAAGAGGAAGAGAGGAAACGGGAAGAGAACACCAGCAACGAGAGTACTGATGTAACA
AAAGGAGACAGCAAAAATGCTAAGAAGAAGAATAACAAGAAAACCAGCAAAAACAAGAGCAGCCTGAGTA
GGGGCAATAAGAAGAAGCCTGGTATGCCCAATGTGTCTAATGACCTTTCTCAGAAACTGTATGCCACCAT
GGAAAAGCATAAAGAGGTTTTCTTTGTGATCCGCCTCATCGCTTGTCCTGCTCCCAATTCCCTGCCTCCC
ATTGTTGATCCTGACCCTCTCATCCCCTGTGACCTGATGGACGGTAGAGATGCATTTCTCACCCTTGCAA
GGGACAAGCACCTGGAGTTCTCTTCACTTCGAAGAGCCCAGTGGTCTACCATGTGCATGCTGGTGGAGCT
CCATACACAGAGTCAGGATCGCTTTGTCTACACCTGCAACGAGTGCAAGCACCACGTGGAGACACGCTGG
CATTGCACTGTGTGTGAGGATTATGACCTGTGCATCACTTGTTATAACACTAAAAATCATGACCACAAAA
TGGAGAAACTAGGCCTTGGCTTAGATGATGAGAGCAATAACCAGCAAGCTGCAGCTACACAGAGCCCAGG
AGACTCCCGCCGCCTGAGCATCCAACGCTGCATCCAGTCTCTGGTGCATGCCTGTCAGTGCCGCAACGCC
AACTGCTCCCTGCCTTCCTGCCAGAAGATGAAGAGGGTTGTGCAGCACACCAAAGGCTGCAAACGGAAAA
CCAATGGTGGGTGCCCCATTTGCAAACAGCTTATTGCCCTCTGCTGCTATCATGCCAAGCACTGCCAGGA
GAACAAGTGCCCGGTGCCATTTTGCCTCAACATCAAGCAAAAGCTCCGGCAGCAACAGCTGCAGCACCGG
CTCCAACAGGCTCAGATGCTCCGTAGGAGGATGGCCAGCATGCAACGAACTGGTGTGGCGGGGCAGCAGC
AGGGCCTGCCGTCCCCAACTCCTGCTACACCAACTACCCCTACTGGCCAACAGCCAGCCACCCCACAGAC
ACCCCAGCCCCAACCCACCTCTCAACCACAGCCCACTCCTCCCAACAACATGACACCCTACTTACCCAGG
ACTCAGACTACTGGCCCTGTGTCCCAGGGTAAGGCACCAGGCCAGGTGACCCCACCTACCCCACCTCAGA
CTGCTCAGGCCCCCCTTCCAGGGCCTCCACCTGCAGCAGTAGAAATGGCAATGCAAATTCAGAGGGCAGC
AGAGACACAGCGCCAGATGGCCCATGTACAGATTTTCCAAAGGCCAATCCAGCACCAGATGCCCCAGATG
TCACCCATGGCCCCTATGGGCATGAACCCACCTCCTATGGCCAGAGGTCCTGGTGGGCATTTGGATCCAG
GAATAGGTCCAGCAGGAATGCAGCAACAGCCACCTTGGGCCCAAGGAGGAATGCCTCAGCCCCAGCAGAT
GCAGTCAGGGATGCCAAGGCCAGCCATGATGTCAGTGGCCCAGCATGGTCAGCCTTTAAACATGGCTCCA
CAACCAGGATTGGGCCAAGTGGGTGTGAGCCCTCTCAAGCCAGGCACTGTGTCTCAACAAGCCTTACAAA
ACCTTTTGCGGACTCTCAGGTCTCCCAGTTCTCCCTTACAGCAGCAACAGGTGCTTAGTATCCTTCATGC
CAACCCCCAACTGTTGGCTGCATTCATCAAGCAGCGGGCTGCCAAGTATGCCAACCCTAATCCACAGCCT
CTCCCTGGACAGCCTGGCATGACCCAGGGACAGCCAGGGCTGCAGCCACCTACCATGCCTGGCCAGCAAG
GTGTCCACTCCAACCCAGCCTTGCAGAACATGAATCCTCTGCAGGCAGGTGTCCAGAGAGCTGGCCTGCC
CCAGCAGCAGCCGCAGCAGCAGCTCCAGCCACCCATGGGAGCAATGAGTCCCCAAGCTCAGCAAATGAAC
ATGAATCACAATACCATGCCTTCACAGTTCAGAGACATCTTAAGACGGCAGATGATGCAACAGCAGGGAG
CAGGGCCAGGAATCGGCCCTGGAATGGCCAACCAGTTCCAGCAGCCCCAAGGAATTGGCTATCCACCGCA
GCAGCAGCAGCAGCAGCGAATGCAGCATCACATGCAGCAAATGCAGCAAGGAAATATGGGACAAATGGGT
CAGCTTCCCCAGGCTCTGGGGGCTGAGGCAGGAGCCAGTCTACAGGCCTATCAGCAGCGACTTCTTCAAC
AACAGATGGGGTCTCCTGCTCAGCCCAACCCTATGAGCCCACAGCAGCACATGCTCCCAAATCAGGCACA
GTCCCCACACCTACAAGGTCAACAAATCCCTAATTCTCTCTCCAATCAAGTGCGCTCTCCCCAGCCTGTC
CCTTCTCCTCGACCACAGTCTCAGCCCCCCCATTCCAGCCCATCTCCGAGGATGCAGCCTCAGCCTTCTC
CACACCATGTTTCTCCACAGACCAGTTCCCCACATCCTGGACTGGTAGCTGCCCAGGCTGCCAACCCTAT
GGAACAAGGGCATTTTGCCAGCCCAGACCAGAATTCAATGCTTTCACAGCTCGCTAGCAATCCTGGCATG
GCAAACCTCCACGGGGCAAGTGCCACGGACCTGGGACTGAGCAGCGATAATGCAGACTTGAATTCAAACC
TCTCACAGAGTACACTAGACATACACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177821
Insert Size 7239 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177821.6, NP_808489.4
RefSeq Size 8749 bp
RefSeq ORF 7239 bp
Locus ID 328572
UniProt ID B2RWS6
Gene Summary Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (By similarity). Acetylates all four core histones in nucleosomes (By similarity). Histone acetylation gives an epigenetic tag for transcriptional activation (By similarity). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein (PubMed:18486321, PubMed:24216764). Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity). Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (By similarity). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2 (PubMed:28883095). Acetylates 'Lys-131' of ALX1 and acts as its coactivator (By similarity). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (By similarity). Acetylates HDAC1 leading to its inactivation and modulation of transcription (By similarity). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (By similarity). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium (By similarity). Promotes cardiac myocyte enlargement (By similarity). Can also mediate transcriptional repression (By similarity). Acetylates FOXO1 and enhances its transcriptional activity (By similarity). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (By similarity). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (By similarity). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:14645221, PubMed:9512516). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (By similarity). Acetylates MEF2D (By similarity). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:27105113). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (By similarity). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:27105113). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (By similarity). Acetylates PCK1 and promotes PCK1 anaplerotic activity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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