Sec16a (NM_153125) Mouse Untagged Clone

CAT#: MC225290

Sec16a (untagged) - Mouse SEC16 homolog A (S. cerevisiae) (Sec16a), (10ug)



  "NM_153125" in other vectors (2)

CNY 37,910.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AU024582; C230052J16Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225290 representing NM_153125
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCACCACCTCAGGCAGTCCCGTCTGGGGTGGCTGGACCACCCCCAGCTGGGAATCCTCGGAGCA
TGTTCTGGGCCAACAGCCCTTACAGGAAGCCAGCCAATAATGCACCAGTGGCTCCCATAACACGCCCATT
GCAGCCAGTAACGGATCCGTTTGCTTTTAATAGACAGACTCTTCAAAACACACCAGTAGGAAGTTCATCC
AAAAGCAGCCTGCCCAATTTGCCTGGCCCAGCCCTCTCTGTTTTTTCTCAGTGGCCTGGTTTGCCTGTGA
CTCCCACAAATGCTGGGGATAGCTCCACAGGATTGCATGAGCCTCTGTCAGGAACTCTGTCACAGCCTAG
AGCAGATGCCAGTCTATTTCCTCCTGCATCTACCCCTTCTTCACTCCCTGGGCTGGAGGTGAGCAGGAAT
GCTGAGGCTGATCCCAGCTCAGGACATGAAGTCCAGATGCTGCCACATTCAGCTCACTACATTCCAGGAG
TGGGTCCTGAGCAGCCTCTCGGGGGCCAAATGAATGACAGTGGGTCCGGGCCTGACCAACCCATGAATAG
GCATGCCCCCCATGATGGTGCCGTGACCCACGCAGCATCTCCTTTCCTCCCTCAGCCACAGATGCCAGGT
CAGTGGGGGCCTGCACAAGGAGGCCCTCAGCCCTCCTATCAGCATCACTCACCCTACCTGGAGGGACCTG
TTCAGAACATGGGGCTCCAGGCTGCCAGCTTGCCCCATTTTCCCCCTCCATCTAGTCTACACCAGGGACC
TGGCCATGAGTCTCATGCCCCACAAACATTTACTCCAGCATCCTTGGCCAGTGGTGAAGGGAATGAGATA
GTTCACCAGCAAAGTAAAAATCACCCATTGAGTAGCTTCCCCCCTAAACACACTTTTGAGCAGAATTCCA
GAATTGGGAATATGTGGGCAAGCCCAGAGTTGAAGCAGAATCCGGGAGTGAATAAAGAGCATCTGCTAGA
CCCTGCCCATGTCAATCCCTTCACTCAGGGAAATAGTCCTGAAAACCAGGCGCATCATCCCCCAGTGGCA
GCAACTAACCATGCCCTGCAAGAAGCAGCCTCTGGAGCTCTCTCAATGTTTTTTCAAGGGGAAGAGACAG
AGAATGAGGAAAATCTCTCATCTGAAAAAGCAGGCCTCGATAAGAGATTGAACTTGGATAGCTTTTCTTC
TACCTCCAGACTGGGCCATCCACCACCACCTGGAGCTAGTGGTGTCTACCAGGCCTTTCCTAGAGGCCCT
AGCAGTGAGGCTGCGCAGGAAGGAGATGCGCAACCTTATTTTTCGCAGTCTGTGGGCGTCCGTCTTGACA
AACAGAGCACTGTGCCCCCTGCTAATGATGCATGGGGTGATGTTCCGGGGACCGGGACCCGCTGTGCCAG
TGGGCCACAGTGTGAGAATGTTGAGAACTTAGAATTTGTTCAGAATCAAGAAGTTTTGCCCCGTGAGACC
CTGAGTGTGGATCCCTTCCCCCTGAGTGATCAGATCCGATATGGGCCCCTTCCTGGGCCAGCTGCCTCCA
GGCCTGCTACTGTGGGCCTCACCAGAGGTGGGGGGCTAAATCTTGAGGCCCCAGACACACCACTGCACCC
TACACGACCTGACAGTGTGTCATCCAGCTACAGCAGCCATAGCCACAGAAGTCCTCCTGGGTCAGCCAGA
CCCCAAGAATTGGTGGGCACATTCATTCAGCAAGAAGTTGGGAAACTTGAAGATGATACTTCAGGGAGTT
TTTTTAAGCAAATTGATTCTTCTCCTGTTGGAGGTGAAACAGATGAGGTGACTGGGAGCCAAAATTGCTG
CAGCAGTCTGTCCCAGCCTTCAACCCCAAGCCCTCCAAAACCTACAGGAGTATTCCAAACAAGTGCAAAC
AGTTCTTTTGAGCCAGTAAAATCCCACTTGGTTGGAGTGAAACCAGTTGAAGCAGATCGTGCCAACATGG
TGGTTGAAGTGAGAGGGACCCAATACTGTCCGAAGAAGCGCAGGGCAGCTGTTGCACCACCTGATGCTAC
CTCAGGAAACCTGGAACAGCCACCAGACAACATGGAGACCCCGTGTGCACCTCAGGCCTGCCCTTTGCCT
CTTAGCACCACTGGAGAGGCTGGGCAACTGGTTTCAAACACGGCAGGGACACCCCTGGATACTGTGCGCC
CAGTGCCTGATAAGAGGCCCTCAGCCAGGGCCCAGGGACCTGTGAAGTGTGAGAGCCCAGCAACAACTCT
GTGGGCACAAAATGAGCTGCCAGATTTTGGTGGCAATGTCCTTCTAGCCCCTGCTGCCCCTGCACTTTAT
GTTCCTGTAAAACCAAAACCTTCAGAGGTTGTTCATCATCCAGAAAAGGGAATGTCTGGACAGAAGGCCT
GGAAACAAGGGTCTGTGCCACCTTTGCAGAACCAAGACCCTCCTGGTGCTTCTGAGAACCTTGAGAACCC
TCCCAAGGTAGGAGAAGAGGAGGCCCTTCCAGTGCAGGCAAGTTCTGGTTATGCGAGTTTGCTTTCTTCG
CCACCCACTGAATCTTTGCACAATCAACCTGTCCTGATTGCTCAGCCTGATCAAAGCTATAATTTGGCTC
AGCCTATTAATTTTTCTGTGTCCTTACTGAATCCTAATGAGAAGAATCAGTCCTGGGGAGATGCTGTGGT
GGGGGAGAGATCCATAGTGAGCAACAACTGGGCTCTCGGTGGTGATCCTGAAGAACGTGCTGCTCTGTCT
GGAGTTCCAGCCAGTGCTGTCACTGGCGCGTCTTTGCCTAGCAGTATCCCCCAGAATTGTGCCCCTCAAG
GTTCGGGGTCTTCTGAAATGATTGCTAGCCAGTCTGCCAGTTGGTTGGTTCAACAGCTATCCCCTCAAAC
CCCTCAAAGTCCGCATCCAAATGCAGAAAAGGGCCCTTCCGAGTTTGTTAGTAGCCCTGCTGGCAATACA
AGTGTGATGCTAGTTCCACCTGCAAGCAGTACTTTGGTTCCTAACAGTAATAAGGCAAAGCACTCTAGTA
ACCAGGAAGAAGCTGTTGGCGCCCTAGACTTCACATTAAATAGAACTTTGGAAAATCCTGTGAGAATGTA
CAGCCCATCTCCTTCAGATGGCCCAGCTTCTCAGCAGCCTCTTCCCAATCATCCCAGACAATCTGGGCCT
GGGCTGCATAACCAGGATCATTTCTACCAGCAGGTAACAAAAGATGCTCAGGACCAGCATAGACTAGAAA
GAGCCCAGCCAGAGCTGGTGCCTCCTCGGCCACAGAATTCTCCCCAAGTGCCCCAAGCATCGTGTCCAGA
GCCTTCAAATCCTGAAAGCCCACCCACACAAGGACAGTCTGAAAGCTTGGCCCAGCCACCAGCAAGTCCA
GCTTCAGTCAACACAGGCCAGCTGCTGCCTCAGCCACCACAGGCTTCCAGTGCATCCGTTACATCTACCA
ACTCCAGCCAGGCAGCTGTGCGGTCAGAGCAGCTGTGGCTACACCCACCACCTCCTAATACTTTTGGCCC
AGCACCTCAAGACTTGGCATCCTACTACTATTACAGACCCCTGTATGATGCCTACCAGTCTCAGTACCCT
TCACCGTACCCGTCAGATCCTGGGACAGCCTCGCTGTACTACCAGGATATGTATGGCCTGTATGAGCCTC
GTTACAGACCCTATGACAGCTCAGCATCTGCTTATGCCGAGAACCACCGCTATTCTGAGCCTGAGCGGCC
CAGCTCCCGTGCAAGTCACTATTCCGACCAGCTTGCTCCCAGGCAAGGATATCCTGAAGGATACTATAAT
TCCAAGAGTGGATGGAGCAGTCACAGTGATTACTATGCAAATTATTACTCTGGCCAGTATGATTATGGAG
ACCCCAGCCGCTGGGATCGTTACTATGGGTCTCGCCTTAGGGATCCTCGCACCTGGGACCGGAGGTACTG
GTATGACTCTGAACATGACCCATACAGGAAGGACCACTATGCTTACAGTGACAGGCCTGAGAAATGTGAT
GATCACTGGAGGTATGACCCTCGCTTCACTGGGAGCTTCGACGATGACGCTGAGATCCACAGGGACCCCT
ATGGAGAAGAAGCAGACAGACGCAGCATCCACAGTGAGCACTCGGCACGGAGCCTGCGCAGCACTCACAG
TCTGCCCAGCCGCCGCAGCAGCCTCAGCTCCCATTCACACCAGAGTCAGATTTACAGAAGCCACCATGTG
ACTGGCGGTTCCTTTGAGGCCCCTCATGCCCCAGGCTCATTTCATGGTGATTATGCCTATGGCACATATG
CCAGCAATTTCAGCGGTGCCCATGGTTTTCCAGAGTACAGCTACCCTGCAGACACCTCCTGGCCTGCTGT
GGAGCAAGTTCCATCAAGACCGACCTCTCCTGAGAAGTTTACGGTGCCTCATGTCTGTGCCAGGTTTGGT
CCTGGTGGTCAGCTCCTTAAAGTGATTCCCAACCTGCCTTCAGAAGGACAGCCTGCACTGGTGGAGATCC
ACAGCTTAGAGACCTTGCTGCAGCACACACCTGAGCAGGAAGAGATGCGCTCCTTCCCAGGACCTCTCGG
CAAAGATGATACCCACAAAGTAGATGTTATTAACTTTGCACAGAACAAAGCAACAAAATGTTTGCAGAAT
GAAAGTTTAATTGATAAAGAGTCTGCAAGTCTTCTTTGGAAATTCATTATTCTGTTATGCAGACAGAATG
GGACTGTTGTGGGAACAGACATTGCAGAGCTTTTGTTACGGGACCATAGAACTGTATGGCTTCCTGGGAA
GTCACCCAATGAGGCAAACCTGATAGATTTTACCAATGAGGCTGTGGAACAAGTGGAAGAGGAGGAGTCA
GGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAGACAGTGACCACCAGCGTGCTGGAGAAGGAGACTG
AGCGGTTCCGTGAGCTCTTGCTGTATGGCCGGAAGAAGGATGCTCTGGAGTCTGCCATGAAAAATGGCTT
ATGGGGTCATGCTCTTCTGCTTGCAAGTAAGATGGACAGCCGGACACATGCCCGTGTCATGACCAGGTTT
GCCAACAGTCTTCCAATCAATGACCCCCTGCAGACTGTCTACCAGCTCATGTCTGGACGGATGCCTGCTG
CATCCACGTGTTGTGGCGATGAGAAGTGGGGAGATTGGAGGCCACATCTTGCAATGGTTTTGTCCAACCT
GAACAACAACATGGATGTTGAATCTAGGACCATGGCTACGATGGGTGATACTCTAGCATCAAAAGGCCTG
TTGGATGCTGCACACTTCTGCTACCTGATGGCCCAGGTTGGATTTGGGGTTTATACAAAGAAAACTACAA
AACTTGTCTTGATAGGCTCAAATCACAGTTTGCCCTTTTTAAAGTTTGCCACCAATGAAGCCATTCAAAG
GACGGAGGCTTATGAGTATGCTCAGTCCCTTGGGGCACATACCTGTTCCTTACCTAACTTCCAGGTGTTT
AAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGACTTGCCACACAGGCCTTCCACTACTGCGAAGTAA
TAGCCAAGAGTGTCCTGACACAGCCTGGTGCATACTCTCCAGTACTGATTAGCCAGTTGACTCAGATGGC
TTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGAGAAGCCAGAAGAGGAGTCCTTTGTGGAGCCTGCC
TGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATCCAGGAGGGCACTGTGCTGTGGAGCCAGGATGGAA
CTGAACCCCAGCAGTGTCGCATTACATCGGGCTCTGAGGTGGAGCAGTCAGATGGCCCTGGACTCAACCA
GCAGGCAGGGCCACAGGCCGACAACCCTCTACTGATGCCAAGCACTGAGCCTTTGATGCATGGCGTGCAA
CTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGCCAGCCTGCTCACCTTTCCAGGGTGCCGATGTTCC
CAGTGCCAATGTCTCGTGGGCCCCTGGAGCTGAGTCCTGCCTATGGACCCCCAGGATCTGCACTTGGCTT
CCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCATCCTGGGCAGGCCCTGCCACCCACTACACTAAGT
CTCCAGGAAAGTGGGCTTCCACCCCAGGAGGCCAAAAGTCCAGACCCAGAAATGGTGCCACGGGGCTCAC
CTGTCAGACACTCCCCTCCGGAGCTCAGTCAAGAGGAGTTTGGCGAGAGCTTCGCTGACCCGGGCTCTTC
CAGAACAGCACAGGACTTGGAAACCTCCCCAGTGTGGGATCTTGGCAGCTCCAGTTTGACACGTGCACCA
TCTTTGACATCTGATTCTGAAGGGAAGAAACCTGCACAGGCTGTCAAAAAGGAGCCCAAGGAGCCCAAGA
AGACTGAGTCCTGGTTCTCTCGTTGGCTGCCTGGAAAAAAAAGGACAGAAGCTTATCTACCAGATGACAA
GAACAAATCAATTGTTTGGGATGAAAAGAAAAACCAGTGGGTGAATTTGAATGAACCAGAGGAGGAGAAG
AAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGGGTTCCCCAGGTGGCTCCCACTGGGCCTGCAGGAC
CACCCACGGCCTCCGTGAATGTGTTTTCTAGAAAAGCAGGTGGGTCCAGAGCTCGCTATGTGGATGTTCT
AAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCTTGCTCCTGCAGATTTCTTTGCTCCTCTCGCCCCA
CTGCCAATTCCTTCTAACTTATTTGTACCAAACCCAGATGCAGAAGAGCCACAACCTGCAGATGGGACTG
GCTGTAGGGGACAGGCACCAGCTGGGACTCAGTCTAAGGCAGAGTCCACCCTGGAACCCAAGGTGGGAAG
TTCTACAGTATCAGCCCCTGGACCTGAGCTCTTACCCTCCAAACCAGATGGCTCCCAAGGAGGAGAGGCT
CCTGGAGACCATTGTCCTACAGGGGCCCCTCATGGTGGGTCTGTGCCCTTTTACAACCCTGCTCAGTTGG
TGCAGGCTTCTGTCACCTCGGGAAATTCAAGGCCAGGGAGGATTGGCCAAAGAAAATATGCGGCATTGAA
CTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_153125
Insert Size 7074 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153125.2, NP_694765.2
RefSeq Size 8754 bp
RefSeq ORF 7074 bp
Locus ID 227648
UniProt ID E9QAT4
Gene Summary Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:17428803). Mediates the recruitment of MIA3/TANGO to ERES. Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane (By similarity). Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in adipocytes (PubMed:27354378).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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