Sec16a (NM_153125) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU024582; C230052J16Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225290 representing NM_153125 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCACCACCTCAGGCAGTCCCGTCTGGGGTGGCTGGACCACCCCCAGCTGGGAATCCTCGGAGCA TGTTCTGGGCCAACAGCCCTTACAGGAAGCCAGCCAATAATGCACCAGTGGCTCCCATAACACGCCCATT GCAGCCAGTAACGGATCCGTTTGCTTTTAATAGACAGACTCTTCAAAACACACCAGTAGGAAGTTCATCC AAAAGCAGCCTGCCCAATTTGCCTGGCCCAGCCCTCTCTGTTTTTTCTCAGTGGCCTGGTTTGCCTGTGA CTCCCACAAATGCTGGGGATAGCTCCACAGGATTGCATGAGCCTCTGTCAGGAACTCTGTCACAGCCTAG AGCAGATGCCAGTCTATTTCCTCCTGCATCTACCCCTTCTTCACTCCCTGGGCTGGAGGTGAGCAGGAAT GCTGAGGCTGATCCCAGCTCAGGACATGAAGTCCAGATGCTGCCACATTCAGCTCACTACATTCCAGGAG TGGGTCCTGAGCAGCCTCTCGGGGGCCAAATGAATGACAGTGGGTCCGGGCCTGACCAACCCATGAATAG GCATGCCCCCCATGATGGTGCCGTGACCCACGCAGCATCTCCTTTCCTCCCTCAGCCACAGATGCCAGGT CAGTGGGGGCCTGCACAAGGAGGCCCTCAGCCCTCCTATCAGCATCACTCACCCTACCTGGAGGGACCTG TTCAGAACATGGGGCTCCAGGCTGCCAGCTTGCCCCATTTTCCCCCTCCATCTAGTCTACACCAGGGACC TGGCCATGAGTCTCATGCCCCACAAACATTTACTCCAGCATCCTTGGCCAGTGGTGAAGGGAATGAGATA GTTCACCAGCAAAGTAAAAATCACCCATTGAGTAGCTTCCCCCCTAAACACACTTTTGAGCAGAATTCCA GAATTGGGAATATGTGGGCAAGCCCAGAGTTGAAGCAGAATCCGGGAGTGAATAAAGAGCATCTGCTAGA CCCTGCCCATGTCAATCCCTTCACTCAGGGAAATAGTCCTGAAAACCAGGCGCATCATCCCCCAGTGGCA GCAACTAACCATGCCCTGCAAGAAGCAGCCTCTGGAGCTCTCTCAATGTTTTTTCAAGGGGAAGAGACAG AGAATGAGGAAAATCTCTCATCTGAAAAAGCAGGCCTCGATAAGAGATTGAACTTGGATAGCTTTTCTTC TACCTCCAGACTGGGCCATCCACCACCACCTGGAGCTAGTGGTGTCTACCAGGCCTTTCCTAGAGGCCCT AGCAGTGAGGCTGCGCAGGAAGGAGATGCGCAACCTTATTTTTCGCAGTCTGTGGGCGTCCGTCTTGACA AACAGAGCACTGTGCCCCCTGCTAATGATGCATGGGGTGATGTTCCGGGGACCGGGACCCGCTGTGCCAG TGGGCCACAGTGTGAGAATGTTGAGAACTTAGAATTTGTTCAGAATCAAGAAGTTTTGCCCCGTGAGACC CTGAGTGTGGATCCCTTCCCCCTGAGTGATCAGATCCGATATGGGCCCCTTCCTGGGCCAGCTGCCTCCA GGCCTGCTACTGTGGGCCTCACCAGAGGTGGGGGGCTAAATCTTGAGGCCCCAGACACACCACTGCACCC TACACGACCTGACAGTGTGTCATCCAGCTACAGCAGCCATAGCCACAGAAGTCCTCCTGGGTCAGCCAGA CCCCAAGAATTGGTGGGCACATTCATTCAGCAAGAAGTTGGGAAACTTGAAGATGATACTTCAGGGAGTT TTTTTAAGCAAATTGATTCTTCTCCTGTTGGAGGTGAAACAGATGAGGTGACTGGGAGCCAAAATTGCTG CAGCAGTCTGTCCCAGCCTTCAACCCCAAGCCCTCCAAAACCTACAGGAGTATTCCAAACAAGTGCAAAC AGTTCTTTTGAGCCAGTAAAATCCCACTTGGTTGGAGTGAAACCAGTTGAAGCAGATCGTGCCAACATGG TGGTTGAAGTGAGAGGGACCCAATACTGTCCGAAGAAGCGCAGGGCAGCTGTTGCACCACCTGATGCTAC CTCAGGAAACCTGGAACAGCCACCAGACAACATGGAGACCCCGTGTGCACCTCAGGCCTGCCCTTTGCCT CTTAGCACCACTGGAGAGGCTGGGCAACTGGTTTCAAACACGGCAGGGACACCCCTGGATACTGTGCGCC CAGTGCCTGATAAGAGGCCCTCAGCCAGGGCCCAGGGACCTGTGAAGTGTGAGAGCCCAGCAACAACTCT GTGGGCACAAAATGAGCTGCCAGATTTTGGTGGCAATGTCCTTCTAGCCCCTGCTGCCCCTGCACTTTAT GTTCCTGTAAAACCAAAACCTTCAGAGGTTGTTCATCATCCAGAAAAGGGAATGTCTGGACAGAAGGCCT GGAAACAAGGGTCTGTGCCACCTTTGCAGAACCAAGACCCTCCTGGTGCTTCTGAGAACCTTGAGAACCC TCCCAAGGTAGGAGAAGAGGAGGCCCTTCCAGTGCAGGCAAGTTCTGGTTATGCGAGTTTGCTTTCTTCG CCACCCACTGAATCTTTGCACAATCAACCTGTCCTGATTGCTCAGCCTGATCAAAGCTATAATTTGGCTC AGCCTATTAATTTTTCTGTGTCCTTACTGAATCCTAATGAGAAGAATCAGTCCTGGGGAGATGCTGTGGT GGGGGAGAGATCCATAGTGAGCAACAACTGGGCTCTCGGTGGTGATCCTGAAGAACGTGCTGCTCTGTCT GGAGTTCCAGCCAGTGCTGTCACTGGCGCGTCTTTGCCTAGCAGTATCCCCCAGAATTGTGCCCCTCAAG GTTCGGGGTCTTCTGAAATGATTGCTAGCCAGTCTGCCAGTTGGTTGGTTCAACAGCTATCCCCTCAAAC CCCTCAAAGTCCGCATCCAAATGCAGAAAAGGGCCCTTCCGAGTTTGTTAGTAGCCCTGCTGGCAATACA AGTGTGATGCTAGTTCCACCTGCAAGCAGTACTTTGGTTCCTAACAGTAATAAGGCAAAGCACTCTAGTA ACCAGGAAGAAGCTGTTGGCGCCCTAGACTTCACATTAAATAGAACTTTGGAAAATCCTGTGAGAATGTA CAGCCCATCTCCTTCAGATGGCCCAGCTTCTCAGCAGCCTCTTCCCAATCATCCCAGACAATCTGGGCCT GGGCTGCATAACCAGGATCATTTCTACCAGCAGGTAACAAAAGATGCTCAGGACCAGCATAGACTAGAAA GAGCCCAGCCAGAGCTGGTGCCTCCTCGGCCACAGAATTCTCCCCAAGTGCCCCAAGCATCGTGTCCAGA GCCTTCAAATCCTGAAAGCCCACCCACACAAGGACAGTCTGAAAGCTTGGCCCAGCCACCAGCAAGTCCA GCTTCAGTCAACACAGGCCAGCTGCTGCCTCAGCCACCACAGGCTTCCAGTGCATCCGTTACATCTACCA ACTCCAGCCAGGCAGCTGTGCGGTCAGAGCAGCTGTGGCTACACCCACCACCTCCTAATACTTTTGGCCC AGCACCTCAAGACTTGGCATCCTACTACTATTACAGACCCCTGTATGATGCCTACCAGTCTCAGTACCCT TCACCGTACCCGTCAGATCCTGGGACAGCCTCGCTGTACTACCAGGATATGTATGGCCTGTATGAGCCTC GTTACAGACCCTATGACAGCTCAGCATCTGCTTATGCCGAGAACCACCGCTATTCTGAGCCTGAGCGGCC CAGCTCCCGTGCAAGTCACTATTCCGACCAGCTTGCTCCCAGGCAAGGATATCCTGAAGGATACTATAAT TCCAAGAGTGGATGGAGCAGTCACAGTGATTACTATGCAAATTATTACTCTGGCCAGTATGATTATGGAG ACCCCAGCCGCTGGGATCGTTACTATGGGTCTCGCCTTAGGGATCCTCGCACCTGGGACCGGAGGTACTG GTATGACTCTGAACATGACCCATACAGGAAGGACCACTATGCTTACAGTGACAGGCCTGAGAAATGTGAT GATCACTGGAGGTATGACCCTCGCTTCACTGGGAGCTTCGACGATGACGCTGAGATCCACAGGGACCCCT ATGGAGAAGAAGCAGACAGACGCAGCATCCACAGTGAGCACTCGGCACGGAGCCTGCGCAGCACTCACAG TCTGCCCAGCCGCCGCAGCAGCCTCAGCTCCCATTCACACCAGAGTCAGATTTACAGAAGCCACCATGTG ACTGGCGGTTCCTTTGAGGCCCCTCATGCCCCAGGCTCATTTCATGGTGATTATGCCTATGGCACATATG CCAGCAATTTCAGCGGTGCCCATGGTTTTCCAGAGTACAGCTACCCTGCAGACACCTCCTGGCCTGCTGT GGAGCAAGTTCCATCAAGACCGACCTCTCCTGAGAAGTTTACGGTGCCTCATGTCTGTGCCAGGTTTGGT CCTGGTGGTCAGCTCCTTAAAGTGATTCCCAACCTGCCTTCAGAAGGACAGCCTGCACTGGTGGAGATCC ACAGCTTAGAGACCTTGCTGCAGCACACACCTGAGCAGGAAGAGATGCGCTCCTTCCCAGGACCTCTCGG CAAAGATGATACCCACAAAGTAGATGTTATTAACTTTGCACAGAACAAAGCAACAAAATGTTTGCAGAAT GAAAGTTTAATTGATAAAGAGTCTGCAAGTCTTCTTTGGAAATTCATTATTCTGTTATGCAGACAGAATG GGACTGTTGTGGGAACAGACATTGCAGAGCTTTTGTTACGGGACCATAGAACTGTATGGCTTCCTGGGAA GTCACCCAATGAGGCAAACCTGATAGATTTTACCAATGAGGCTGTGGAACAAGTGGAAGAGGAGGAGTCA GGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAGACAGTGACCACCAGCGTGCTGGAGAAGGAGACTG AGCGGTTCCGTGAGCTCTTGCTGTATGGCCGGAAGAAGGATGCTCTGGAGTCTGCCATGAAAAATGGCTT ATGGGGTCATGCTCTTCTGCTTGCAAGTAAGATGGACAGCCGGACACATGCCCGTGTCATGACCAGGTTT GCCAACAGTCTTCCAATCAATGACCCCCTGCAGACTGTCTACCAGCTCATGTCTGGACGGATGCCTGCTG CATCCACGTGTTGTGGCGATGAGAAGTGGGGAGATTGGAGGCCACATCTTGCAATGGTTTTGTCCAACCT GAACAACAACATGGATGTTGAATCTAGGACCATGGCTACGATGGGTGATACTCTAGCATCAAAAGGCCTG TTGGATGCTGCACACTTCTGCTACCTGATGGCCCAGGTTGGATTTGGGGTTTATACAAAGAAAACTACAA AACTTGTCTTGATAGGCTCAAATCACAGTTTGCCCTTTTTAAAGTTTGCCACCAATGAAGCCATTCAAAG GACGGAGGCTTATGAGTATGCTCAGTCCCTTGGGGCACATACCTGTTCCTTACCTAACTTCCAGGTGTTT AAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGACTTGCCACACAGGCCTTCCACTACTGCGAAGTAA TAGCCAAGAGTGTCCTGACACAGCCTGGTGCATACTCTCCAGTACTGATTAGCCAGTTGACTCAGATGGC TTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGAGAAGCCAGAAGAGGAGTCCTTTGTGGAGCCTGCC TGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATCCAGGAGGGCACTGTGCTGTGGAGCCAGGATGGAA CTGAACCCCAGCAGTGTCGCATTACATCGGGCTCTGAGGTGGAGCAGTCAGATGGCCCTGGACTCAACCA GCAGGCAGGGCCACAGGCCGACAACCCTCTACTGATGCCAAGCACTGAGCCTTTGATGCATGGCGTGCAA CTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGCCAGCCTGCTCACCTTTCCAGGGTGCCGATGTTCC CAGTGCCAATGTCTCGTGGGCCCCTGGAGCTGAGTCCTGCCTATGGACCCCCAGGATCTGCACTTGGCTT CCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCATCCTGGGCAGGCCCTGCCACCCACTACACTAAGT CTCCAGGAAAGTGGGCTTCCACCCCAGGAGGCCAAAAGTCCAGACCCAGAAATGGTGCCACGGGGCTCAC CTGTCAGACACTCCCCTCCGGAGCTCAGTCAAGAGGAGTTTGGCGAGAGCTTCGCTGACCCGGGCTCTTC CAGAACAGCACAGGACTTGGAAACCTCCCCAGTGTGGGATCTTGGCAGCTCCAGTTTGACACGTGCACCA TCTTTGACATCTGATTCTGAAGGGAAGAAACCTGCACAGGCTGTCAAAAAGGAGCCCAAGGAGCCCAAGA AGACTGAGTCCTGGTTCTCTCGTTGGCTGCCTGGAAAAAAAAGGACAGAAGCTTATCTACCAGATGACAA GAACAAATCAATTGTTTGGGATGAAAAGAAAAACCAGTGGGTGAATTTGAATGAACCAGAGGAGGAGAAG AAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGGGTTCCCCAGGTGGCTCCCACTGGGCCTGCAGGAC CACCCACGGCCTCCGTGAATGTGTTTTCTAGAAAAGCAGGTGGGTCCAGAGCTCGCTATGTGGATGTTCT AAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCTTGCTCCTGCAGATTTCTTTGCTCCTCTCGCCCCA CTGCCAATTCCTTCTAACTTATTTGTACCAAACCCAGATGCAGAAGAGCCACAACCTGCAGATGGGACTG GCTGTAGGGGACAGGCACCAGCTGGGACTCAGTCTAAGGCAGAGTCCACCCTGGAACCCAAGGTGGGAAG TTCTACAGTATCAGCCCCTGGACCTGAGCTCTTACCCTCCAAACCAGATGGCTCCCAAGGAGGAGAGGCT CCTGGAGACCATTGTCCTACAGGGGCCCCTCATGGTGGGTCTGTGCCCTTTTACAACCCTGCTCAGTTGG TGCAGGCTTCTGTCACCTCGGGAAATTCAAGGCCAGGGAGGATTGGCCAAAGAAAATATGCGGCATTGAA CTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153125
Insert Size	7074 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153125.2, NP_694765.2
RefSeq Size	8754 bp
RefSeq ORF	7074 bp
Locus ID	227648
UniProt ID	E9QAT4
Gene Summary	Acts as a molecular scaffold that plays a key role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:17428803). Mediates the recruitment of MIA3/TANGO to ERES. Regulates both conventional (ER/Golgi-dependent) and GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane (By similarity). Acts as a RAB10 effector in the regulation of insulin-induced SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in adipocytes (PubMed:27354378).[UniProtKB/Swiss-Prot Function]

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