Prpf8 (NM_138659) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU019467; D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225282 representing NM_138659 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGTGTTTCCTTACCGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT ATATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGATATGCAGA GAAGCGGAAATTTGGGTTTGTGGATGCTCAGAAGGAAGACATGCCACCAGAACATGTCAGGAAGATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTTAAGT ACATGCCTCATGCAGTCCTCAAGCTCCTTGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGACGTGCC TGTGCTGTACCACATCACTGGAGCCATCTCCTTTGTTAACGAGATTCCCTGGGTTATCGAGCCGGTTTAC ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA TGCGTTTCCCCCCTTTCGATGACGAGGAACCGCCCCTGGATTATGCCGACAACATCTTAGATGTTGAGCC ACTGGAAGCCATTCAGCTAGAGCTGGACCCAGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCTTTGAGAGACAGTAGGAAGTACGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA TGATGTCCACACTCTACCGACTGGCCAATCAGCTTCTGACTGACCTTGTAGATGACAACTACTTCTATCT CTTTGATCTGAAGGCCTTTTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAG CCTCTTGTTCGAGACATCAACTTACAGGATGAAGACTGGAATGAATTTAACGATATTAATAAGATCATCA TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCTTACCTGTATAACAACCTTCCACACCACGT CCACCTGACCTGGTACCACACTCCCAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC TACTTTGATCCTTTGATCAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAACCACTGCCTGATG ATGATGAAGAGTTTGAACTCCCAGAGTTTGTCGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA CACAGCCAATGGCATTGCCCTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG GCCTTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAGCCTGTGAAGGTTC GAGTCTCTTATCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTCAAGCACCGGCCTCCCAAAGCCCA GAAGAAGAGGTATCTGTTCCGTTCTTTCAAAGCTACCAAATTCTTTCAGTCCACCAAGCTGGACTGGGTG GAGGTAGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGGAAGAACCTCA ACTATCTGCACTTGGACTACAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAGAA ATCCCGTTTTGGGAATGCTTTCCATCTATGTCGGGAAGTACTGCGTTTAACCAAGCTGGTTGTGGACAGC CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGTTTGCAGTATATATTTGCCC ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTAGGGAAAGGCCCTGGCTGTGGTTTCTGG GCTGCTGGCTGGCGGGTCTGGCTGTTCTTTATGCGTGGAATTACCCCTCTGCTAGAGCGATGGCTGGGCA ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACGAAGCAGCGGGT GGAGTCGCACTTTGACCTGGAGCTCAGGGCTGCTGTGATGCACGACATTCTGGACATGATGCCTGAAGGC ATCAAGCAGAACAAGGCCCGGACAATTCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAGGCCAACA TCCCTTGGAAGGTCCCAGGACTGCCAACACCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC TGATTGGTGGACCAATACTGCCCACTACAACCGGGAACGGATCCGTCGCGGGGCTACTGTAGACAAGACT GTTTGCAAAAAGAACCTGGGCCGCCTCACTCGCCTGTATCTGAAGGCAGAGCAGGAGCGGCAGCACAATT ACCTGAAGGATGGGCCTTACATCACAGCCGAGGAGGCAGTGGCCGTGTATACCACCACTGTGCACTGGCT GGAGAGCCGGAGATTCTCGCCCATCCCCTTCCCTCCACTCTCCTACAAGCATGATACCAAGCTGCTCATC TTGGCTCTGGAGCGTCTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC ACAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTACCAGTGTATGAT GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC GTCTTTTCCCACCCTGGATCAAGCCTGCTGACACAGAGCCACCTCCACTGCTTGTTTACAAGTGGTGTCA AGGCATTAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGCGAGTGCAACGTCATGCTGGAGTCTCGG TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTTAACAGGCTGCTGCGACTCATTGTGGACCACA ACATAGCTGACTACATGACGGCCAAGAACAACGTAGTCATCAACTATAAGGACATGAACCATACAAACTC ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGCTTGGTGATGGATTTG CTTGTACTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCTCAGATGCCAAATGACTTTCTCAGTT TCCAGGACATAGCCACTGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACACCCAGACCCC AACAATGAAAACATTGTTGGCTACAATAACAAGAAATGCTGGCCCCGAGATGCCCGAATGCGGCTCATGA AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTATTCAACATGTGT GGCTTTGAGTGCCGCATCCTGCCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT GGAACCTACAGAATGAGGTTACCAAGGAGCGGACAGCTCAGTGCTTCCTGCGTGTGGATGATGAGTCAAT GCAACGCTTCCATAACCGTGTGCGGCAGATTCTGATGGCCTCTGGCTCCACCACGTTCACCAAGATTGTG AATAAGTGGAACACAGCTCTCATCGGCCTTATGACATATTTTCGAGAGGCTGTGGTGAATACACAGGAAC TCCTGGACTTATTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT GCCTAGCCGGTTCCCGCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG GGCCATGTGCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAGACAGATGTGGGCATCACACACTTCC GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG CGAGTTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGAGACAAGAGGCTATTGCTCAGAAC AGACGCTTGACTTTAGAAGATTTAGAAGATTCATGGGATCGAGGCATTCCCCGGATCAATACCCTCTTCC AGAAAGACCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT TTTGAAGCAGAACCCCTTTTGGTGGACACATCAGCGGCATGATGGCAAGCTTTGGAACCTCAATAACTAC CGCACAGACATGATTCAGGCGCTGGGTGGTGTGGAGGGCATTCTGGAGCACACTCTCTTCAAGGGCACTT ACTTCCCCACCTGGGAGGGTCTGTTCTGGGAGAAAGCCAGTGGCTTTGAGGAGTCCATGAAGTGGAAGAA GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACCCTCTGGTGGTCCCCA ACCATCAATCGAGCCAATGTCTATGTGGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTCATGCACG GCAAGATCCCCACACTGAAGATCTCTCTCATCCAGATATTCCGAGCTCACTTGTGGCAGAAGATCCATGA GAGCATCGTGATGGACTTGTGTCAGGTCTTTGACCAGGAACTGGATGCACTGGAAATTGAGACAGTGCAA AAGGAGACAATCCATCCCCGAAAGTCCTATAAGATGAACTCTTCCTGTGCGGATATACTACTCTTTGCCT CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCCAAGGATGTGATGGACAGCACCACCAC ACAGAAGTATTGGATTGACATCCAATTACGCTGGGGAGACTATGATTCCCATGATATTGAGCGCTATGCC CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCGCCCACAGGCGTGCTCATTG CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGCAGCAAGCCTCTCATACA GCAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCGTTGTATGTGTTACGTGAACGGATACGCAAAGGA TTACAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCTA ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA CTTGACAACTAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGAACGGGACAGCTGTTCCTCAAG ATAATCCACACATCTGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAGG AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATCATTGTCACTCGGAAGGG CATGCTGGATCCTCTGGAGGTCCACTTACTGGATTTCCCCAATATTGTGATCAAAGGATCGGAGCTCCAG CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACCGAGCCCCAGA TGGTCCTTTTCAACCTCTATGATGACTGGCTCAAGACTATCTCCTCCTACACGGCTTTCTCCCGTCTCAT TCTGATTCTGCGGGCTTTGCATGTGAACAATGATAGAGCAAAGGTGATCCTGAAGCCAGACAAGACTACT GTTACGGAACCCCACCACATCTGGCCCACGCTGACTGACGAGGAATGGATCAAGGTGGAGGTACAGCTCA AGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAACGTGAATGTGGCGTCACTGACACAGTCAGAAAT TCGAGACATCATCCTGGGAATGGAGATCTCGGCGCCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAA AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCACGGTGACGAGA TTATCACTTCAACCACTAGCAACTATGAGACCCAGACCTTCTCATCAAAGACTGAATGGAGAGTCAGGGC CATCTCTGCTGCCAACCTGCATCTGCGGACCAATCATATTTATGTTTCATCTGATGACATTAAGGAGACT GGTTATACGTACATTCTTCCGAAAAACGTGCTTAAGAAGTTCATCTGCATATCTGATCTTCGTGCCCAAA TTGCAGGATACCTGTATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGTTGTATTGTGAT GGTGCCGCAGTGGGGCACGCACCAGACTGTGCACCTGCCCAGCCAGCTGCCCCAGCATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAATCCCCCCAGCTATCACCCCAAGATGTCA CCACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACGTGCAG CTTCACACCAGGCTCCTGCACACTGACAGCCTACAAGCTGACACCCAGTGGGTATGAGTGGGGCCGGCAG AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCCTCGCACTACGAGAGGGTTCAGATGCTCCTGT CTGACCGATTCCTCGGCTTCTTCATGGTCCCCGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG ACATGATCCCAACATGAAATACGAGCTGCAGCTGGCAAACCCCAAGGAATTCTACCATGAGGTCCACAGG CCCTCCCACTTCCTCAACTTCGCCCTTCTGCAGGAGGGTGAGGTCTACTCTGCAGACAGAGAGGACCTCT ATGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_138659
Insert Size	7008 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138659.2, NP_619600.2
RefSeq Size	7500 bp
RefSeq ORF	7008 bp
Locus ID	192159
UniProt ID	Q99PV0
Gene Summary	Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.[UniProtKB/Swiss-Prot Function]

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