Cacna1b (NM_007579) Mouse Untagged Clone

CAT#: MC225258

Cacna1b (untagged) - Mouse calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 2, (10ug)



  "NM_007579" in other vectors (2)

CNY 36,670.00


货期*
13周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms alpha(1B); AW050276; AW060892; AW822256; BIII; Cav2.2; Cchn1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225258 representing NM_007579
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGTG
GCGGGGCCGGCGGGGCGGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA
GTCCATTGCGCAGCGCGCACGGACTATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC
GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCTAAGCGCATCACCGAAT
GGCCGCCCTTCGAATACATGATCCTGGCCACCATCATCGCCAACTGCATTGTTCTGGCCCTGGAGCAGCA
CCTCCCTGATGGGGACAAGACTCCCATGTCTGAGCGACTAGATGACACGGAGCCTTACTTCATCGGGATC
TTTTGCTTCGAGGCGGGCATCAAGATCATAGCCCTGGGCTTTGTTTTCCACAAGGGCTCCTACCTTCGGA
ACGGCTGGAATGTCATGGACTTCGTGGTGGTCCTCACGGGGATTCTCGCCACAGCTGGAACTGACTTTGA
CCTGCGCACACTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGCTGGTGTCTGGAATTCCAAGCTTGCAG
GTGGTGCTTAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATTGGGCTGCTGCTCTTCTTTGCCA
TCCTCATGTTTGCCATCATCGGCCTCGAATTCTATATGGGCAAATTCCATAAGGCCTGTTTCCCCAACAG
CACAGATACAGAGCCTGTGGGTGACTTTCCCTGTGGCAAAGATCCCCCTGCTCGTCAGTGTGATGGTGAC
ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA
TCTTGACAGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC
TGGCAACACATGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG
GTGCTGGGTGTGCTTTCCGGAGAGTTTGCCAAGGAGCGGGAGCGAGTCGAGAACCGCCGCGCCTTCCTGA
AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAGCTGAATGGGTACTTGGAGTGGATCTTCAAGGCAGAGGA
AGTCATGTTGGCAGAGGAGGACAAGAATGCAGAAGAGAAATCCCCTTTGGATGTGTTGAAGAGAGCTGCC
ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCAG
TTGGGTCTCCATTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACGGAGAGCTCATCGTACTTCCGGAGAAA
GGAGAAGATGTTCCGGTTCTTTATCCGGCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGT
GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCACTATAATCAGCCTCAGCGGCTTACCACTGCAC
TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTAGG
GCCCAGAAGTTACTTCAGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA
GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTTGGAATCAGTGTGCTGCGGGCTCTGCGACTGCTGA
GGATATTCAAAGTTACCAAGTATTGGAACTCTCTGAGGAACCTGGTGGTTTCCCTCCTCAATTCCATGAA
GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTCTTCATCGTGGTCTTCGCTCTGTTGGGGATGCAGCTG
TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC
TCACTGTCTTTCAGATCCTGACAGGAGAGGATTGGAATGCCGTAATGTATCATGGGATTGAGTCGCAAGG
TGGAGTCAGCAAAGGCATGTTTTCTTCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG
CTGAATGTTTTTCTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG
AGATGGAAGAAGCAGCCAATCAGAAACTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT
GTCTGCTGCCAATATCTCCATCGCTGCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGG
GCCAGTCAGCTAAGGCTCCAGAATCTGCGTGCCAGCTGTGAGGCATTGTACAGTGAGATGGACCCTGAGG
AGCGCCTGCGTTATGCCAGCACGCGCCATGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGT
GGTGGAGCCTGGTCGAGATGGCTTGCGGGGACCCGTTGGGAGCAAGTCAAAGCCTGAAGGCACGGAGGCC
ACAGAAAGCGCGGACCTACCTCGCAGGCACCACCGGCACCGTGATAGGGACAAGACCTCAGCCACAGCAC
CTGCTGGAGGCGAACAGGACAGGACAGAAAGCACCGAGACCGGGGCCCGGGAGGAACGTGCGCGCCCTCG
TCGAAGTCACAGCAAGGAGACTCCAGGGGCTGACACGCAAGTGCGCTGTGAGCGCAGTAGACGTCACCAC
CGGCGCGGCTCCCCGGAGGAGGCCACTGAACGGGAGCCTCGGCGCCACCGTGCCCACCGGCATGCACAGG
ACTCAAGCAAGGAGGGCACGGCGCCGGTGCTTGTACCCAAGGGTGAGCGACGAGCAAGACACCGAGGCCC
ACGCACGGGTCCACGTGAGGCAGAGAACAACGAGGAGCCCACACGCAGGCACCGTGCAAGGCATAAGGTG
CCACCCACACTGCAGCCCCCAGAGAGGGAGGCTGCAGAGAAGGAGAGCAACGCGGTGGAAGGGGATAAGG
AAACCCGAAATCACCAGCCCAAGGAACCTCACTGTGACCTGGAGGCCATTGCAGTTACAGGTGTGGGCCC
TCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAGCAACCAGAGGATGCAGACAACCAGCGT
AATGTCACCCGGATGGGCAGTCAGCCCTCAGATCCCAGCACCACTGTGCATGTCCCAGTGACACTGACAG
GCCCTCCTGGGGAGACCCCTGTAGTTCCCAGTGGTAACATGAACCTGGAAGGCCAAGCAGAGGGCAAGAA
GGAGGCAGAGGCGGATGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTCCCTACAGCTCCATGTTTTGT
CTCAGCCCCACCAACCTGCTTCGTCGCTTCTGCCATTACATTGTGACCATGCGGTACTTTGAGATGGTAA
TTCTTGTGGTCATTGCCTTGAGCAGCATTGCCCTGGCTGCAGAGGATCCTGTGCGGACAGATTCATTCAG
GAACAACGCTTTAAAGTACATGGATTACATCTTTACAGGAGTCTTCACCTTTGAAATGGTCATAAAGATG
ATAGACTTGGGCTTGCTGCTGCACCCTGGTGCCTACTTCCGGGACCTGTGGAACATTCTGGACTTCATTG
TTGTCAGTGGAGCCCTGGTGGCATTTGCGTTCTCAGGATCCAAAGGGAAAGACATCAATACCATCAAGTC
TCTGAGAGTCCTGCGTGTCCTGAGGCCCCTCAAGACCATCAAGCGGCTGCCTAAGCTCAAGGCTGTCTTT
GACTGTGTGGTGAACTCCCTGAAGAACGTCTTGAACATCCTGATTGTCTACATGCTCTTCATGTTCATAT
TTGCCGTCATTGCCGTCCAGCTCTTCAAAGGGAAGTTCTTTTACTGTACTGATGAATCCAAGGAGCTGGA
GAGGGACTGCCGGGGTCAGTATTTGGATTATGAGAAGGAAGAAGTAGAAGCCCAGCCAAGGCAGTGGAAG
AAATATGACTTCCACTATGACAATGTTCTCTGGGCCTTGTTGACGCTGTTCACAGTGTCCACGGGAGAGG
GGTGGCCCATGGTGCTGAAACACTCTGTGGATGCCACCTATGAGGAACAGGGGCCCAGTCCCGGGTTCCG
GATGGAGCTCTCCATCTTCTACGTGGTCTACTTTGTGGTCTTCCCTTTTTTCTTTGTCAACATCTTTGTG
GCCTTGATCATTATCACCTTCCAGGAACAGGGAGATAAGGTGATGTCTGAATGCAGCTTAGAAAAGAATG
AGAGGGCTTGCATTGATTTTGCCATCAGTGCCAAGCCCCTGACACGGTACATGCCTCAAAACAAACAGTC
GTTCCAGTATAAGACATGGACATTCGTGGTCTCTCCACCCTTTGAGTACTTCATCATGGCTATGATAGCC
CTCAACACAGTGGTGCTGATGATGAAGTTCTATGATGCACCTTATGAGTACGAGCTGATGCTGAAATGCC
TGAACATTGTCTTCACATCCATGTTCTCGATGGAGTGCATACTGAAGATCATCGCCTTTGGGGTATTGAA
CTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACGGTTTTGGGAAGTATTACTGATATTTTAGTA
ACAGAGATAGCGGAAACGAACAACTTCATCAACCTAAGCTTCCTTCGCCTCTTCCGGGCGGCACGGCTGA
TCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCCTATTGTGGACCTTCGTCCAGTCCTTTAAGGCGCT
GCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCATCGGCATGCAGGTTTTTGGA
AACATTGCCCTTGATGATGACACCAGTATCAACCGACACAACAACTTCCGGACATTTCTGCAAGCCTTAA
TGCTATTGTTCAGGAGTGCCACTGGGGAGGCCTGGCATGAGATCATGCTGTCTTGTCTGGGCAACCGGGC
CTGTGACCCACATGCCAACGCCAGTGAGTGCGGGAGCGACTTTGCCTATTTTTATTTTGTCTCCTTCATC
TTCCTCTGTTCCTTTCTGATGTTGAACCTCTTTGTTGCTGTAATCATGGACAATTTTGAGTACCTCACTC
GGGACTCTTCCATCCTAGGGCCTCACCACTTAGACGAATTCATTCGAGTCTGGGCTGAATACGACCCAGC
TGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACATGTCCCCACCTCTGGGGTTG
GGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGCATGAACATGCCCATATCCAATGAGG
ACATGACGGTGCACTTTACGTCCACACTGATGGCCCTCATCCGGACAGCACTGGAGATCAAGCTTGCCCC
AGCTGACGAGATGACAGTGGGGAAGGTCTATGCTGCTCTCATGATATTTGACTTCTACAAACAGAACAAA
ACCACCAGAGATCAGACTCACCAAGCTCCCGGAGGCCTGTCCCAGATGGGTCCCGTTTCCCTGTTCCACC
CTCTGAAGGCCACCCTGGAACAGACACAGCCCGCTGTGCTTCGAGGAGCTCGGGTTTTCCTTCGGCAAAA
GAGTGCAACTTCCCTCAGCAATGGGGGTGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCGCTGTCC
TGGGGCACGCAGAGGACCCAAGATGCACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAG
AGATCCCTGTGGGGCAGTCAGGAACACTGGCTGTGGATGTCCAGATGCAGAACATGACACTGAGAGGACC
AGATGGGGAGCCCCAGCCTGGGCTGGAAAGCCAAGGCAGAGCTGCCTCTATGCCACGCCTAGCGGCAGAA
ACACAGCCGGCCCCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCACATGGGA
CTCAGCTTTGCAGCACAGTTCTGGACCGGCCTCCTCCTAGCCAGGCATCACATCACCACCACCACCGCTG
CCACCGGCGCAGAGACAAGAAGCAAAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACCCAGAAGGT
GCACCAAGCACTGCTGCAGGACCTGGTCTGCCCCATGGAGAAGGATCCACCGCCTGCCGGCGGGACCGTA
AACAGGAGCGAGGCCGGTCCCAGGAGCGGAGGCAGCCCTCATCTTCCTCTTCAGAGAAGCAGCGCTTCTA
TTCCTGTGACCGCTTTGGGAGCCGGGAGCCCCCGCAACTGATGCCCTCACTCAGTAGCCACCCCACATCG
CCAACAGCGGCGCTAGAGCCAGCACCCCACCCACAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGT
CAACATCCGGTGCTAGCACCCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCTCTGACCCCACG
CCCCAGCATCACCTACAAGACCGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGCGGCCTCCCA
GCCTTCTCCCCTGGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGA
GCCAGCCTCTAGCTCCTGGCTCCCGAATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGC
CTCCGCCCACACCCTGCCTGAGGATACACTCACCTTTGAAGAGGCAGTGGCCACCAACTCTGGCCGCTCC
TCCAGGACTTCCTATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTATC
ACTGCACTTTGGGACTCAGCACTGGCGTCCGGGCGCGGCACAGCTACCACCACCCCGATCAGGACCACTG
GTGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007579
Insert Size 6867 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007579.3, NP_031605.2
RefSeq Size 9655 bp
RefSeq ORF 6867 bp
Locus ID 12287
Gene Summary Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by omega-conotoxin-GVIA (AC P01522) (By similarity). They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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