Med12l (NM_177855) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A130035F20; A630079M02; AU044581; TRALPUSH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225217 representing NM_177855 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCTTCGGGCTTCTCAGCTATGAGCAGAGACCGCTGAAGCGCCCGCGGCTCGGGCCGCCCGACG TCTACCCGCAGGACCCTAAGCAGAAGGAGGATGAACTTACAGCTGTGAATGTAAAGCAGGGATTCAACAA CCAGCCGGCCTTCACTGGAGACGAACATGGCTCAGCCAGAAACATTGTAATCAACCCATCCAAGATCGGT GCGTATTTCAGCAGCATATTGGCCGAGAAACTGAAGCTTAACACTTTCCAGGACACCGGAAAGAAGAAAC CCCAAGTTAATGCTAAAGATAATTACTGGCTGGTTACTGCCCGATCGCAGAGTGCAATTCATAGTTGGTT CTCTGACCTAGCAGGGAATAAACCACTTGCTATCTTGGCAAAAAAGGTCCCCATCCTCAGTAAAAAAGAG GACGTCTTTGCATATTTAGCTAAGTATTCTGTGCCAATGGTTCGAGCAACGTGGCTGATCAAGATGACTT GTGCCTATTACTCTGCTATTTCTGAAGCTAAAATTAAGAAACGCCAGGCTCCCGATCCCAATTTGGAGTG GACACAGATATCTACCAGATACCTCAGAGAGCAGTTGGTCAAAATCTCTGACTTTTACCACATGGCTTCC AGTACAGGTGATGGTCCTGTCCCTGTGCCACCAGAGGTTGAGCAAGCCATGAAGCAGTGGGAGTACAACG AGAAGCTGGCATTTCATATGTTCCAGGAAGGAATGTTGGAAAAGCACGAGTATTTGACTTGGATCCTGGA CGTCTTAGAGAAGATCAGACCAGTGGATGACAGCCTTCTGAAGCTACTTTTGCCCCTGATGTTGCAGTAT TCAGATGAGTTTGTTCAGTCAGCCTACCTGTCTCGTCGTCTCGCGTACTTTTGTGCCCGGCGTCTTTCCT TGCTACTGAGTGATAGCCCCAACCTCCTTGCTGCTCACTCGCCCCACATGATAATCGGAACAAACAACAC GAGTATTGGGACCCCCAGCCCTGGCACCCCTGGCCCTGGCATGAGCCCTGTGCAGCTGGCCTTCTCGGAT TTCCTTTCCTGTGCACAGCATGGCCCCCTGGTTTATGGACTTAGTTGTATGTTGCAGACTGTCACTCTGT GTTGCCCAAGTGCCTTGGTGTGGAATTATTCCACCAATGAAAATAAGATCTCGAATCCAGGCTCTCCCCT GGATCTGCTGCAGGTCGCCCCGTCCAGCCTCCCCATGCCGGGTGGGAACACGGCTTTCAATCAGCAGGTT CGGGCAAGGATTTATGAGGTAGAACAGCAGATAAAGCAGCGAGGCCGTGCAGTGGAAGTTCGGTGGTCTT TTGATAAGTGTCAGGAGTCGACGGCAGGGGTGACTATTAGTCGGGTCTTGCATACACTGGAAGTCTTAGA TCGTCATTGTTTCGACCGAACTGATTCCAGCAATTCAATGGAGACTCTTTATCATAAAATTTTCTGGGCA AACCAAAACAAAGATAACCAAGAGGTTGCCCCCAATGATGAAGCTGTGGTGACATTGTTATGTGAATGGG CTGTAAGCTGCAAAAGGTCTGGTAAGCACAGGGCCATGGCTGTGGCGAAACTCTTGGAGAAAAGGCAAGC GGAGATTGAGGCAGAGAGATGTGGGGAATCAGAAGTCCTAGATGAGAAAGAGTCAATTTCTTCAGCATCT CTTGCAGGATCTAGTTTGCCTGTTTTCCAGAATGTTTTGCTGAGGTTTTTAGATACACAGGCCCCAGCGC TGTCGGACCCCAACAGTGAATGCGAGAAGGTAGAGTTTGTGAACTTGGTGCTGCTCTTCTGCGAGTTTAT CCGCCACGATGTCTTCTCCCATGATGCATATATGTGCACCCTCATCTCTCGAGGAGACTTATCAGTTACT GCCTCCACGGGGCTGCGGTCGCCAGCAGGAGAGAATTCAGATGAACACTATTCCAAGGATCATGATATGA AGATGGAGGAGCACAGTGTCATGGCGCGCCTGGGTCTCGGCTCAGGAGCCACTCACATCTTTGATGATGT AGACAAGAGTGACTTTAAAAGTGACCCTGCTTCTGAGTTTCCAATTTTTTCTCCCATGCCTGGAGAATCC TGTGAGAACATCAACCCTTCCTTGAGCAGAAGACTGTCAGTCAATGGTGAGAAACTGCTAAAGAGAGAAA AACCAAGAGAATTAATTTTCCCATCAAATTACGACCTCCTCCGACACCTGCAGTATGCAACCCACTTTCC TATACCTCTGGATGAATCTTCAAGTCATGAATGTAACCAGCGCACAATCCTTCTCTATGGAGTCGGAAAA GAGCGTGATGAAGCCAGGCATCAGCTGAAGAAGATTACCAAAGATATCCTCAAAATCCTGAACAAGAAGG GCACCACAGAATCAGGGGTTGGGGACGAAGGACAGAAAGCCAGGAAGACCAAGCAGGAAGTTTTTCCAAC CCCGGAAAATGTGTTCACGAAACTCCAACTCCTCTCATACTTCGATCAGCATCAAGTAACATCTCAGATC TCTAACAATGTGCTGGAACAAATCACAAGCTTTGCGTCTGGAACATCCTATCACCTCCCGTTGGCTCACC ATATTCAGCTCATCTTTGATCTCATGGAGCCAGCGCTGAACATCAATGGGCTGATTGACTTTGCCATACA GCTGCTGAATGAACTGAGTGTGGTGGAAGCAGAGCTCCTCTTGAAGTCTTCTAGCCTGGCAGGAAGTTAC ACTACAGGACTATGTGTCTGCATTGTGGCCGTGCTCAGGCGGTACCATAGTTGCCTGATCCTAAATCCTG ATCAGACAGCCCAGGTGTTTGAAGGGTTGTGTGGTGTGGTGAAGCACGTTGTCAACCCCTCAGAATGTTC TTCCCCAGAGAGATGTATCTTGGCCTACCTCTATGACCTCTACGTATCCTGCAGCCACCTCAGAAGTAAA TTTGGAGACCTTTTTAGTAGTGCCTGTTCAAAAGTAAAGCAGACCATATACAATAATGTGATGCCTGCAA ATTCCAACTTACGCTGGGATCCAGACTTTATGATGGATTTTATTGAGAACCCCTCGGCAAGAAGCATCAA TTATTCAATGCTGGGCAAGATCCTCAATGACAATGCTGCCAACCGGTACAGCTTTGTGTGCAACACCCTC ATGAATGTGTGCATGGGACACCAGGATGCCGGCCGGATAAATGACATAGCCAATTTCTCCTCTGAGCTAA CTGCCTGCTGTACAGTTCTGAGTTCGGAGTGGCTGGGGGTTCTGAAGGCTCTTTGTTGTTCTTCAAATCA TGTGTGGGGATTTAATGATGTCCTCTGCACGGTGGATGTGAGTGACCTTTCATTCCATGATTCATTAGCT ACTTTCATTGCTATCCTGATAGCACGACAGTGTTTCTCCCTGGAAGATGTCGTGCAACACGTCGCACTTC CCTCTCTCCTAGCAGCAGCTTGTGGAGACGCGGATGCAGAGCCCGGGGCAAGGATGACGTGCCGCCTCCT GCTTCACCTCTTCAGAGCCCCCCAGGCCTGCTTTTTCCCTCAGGGAACAGGCAAACCTTTCCCCGGAATA AGATCATCTTGTGATAGACACCTCTTAGCTGCAGCTCACAACAGCATAGAAGTGGGAGCAGTTTTTGCTG TCTTAAAGGCAATTATGATGCTTGGCGATGCCAAAATTGGCAGTAACAATGTCAACACGATGAAGAATGA AGACTTCGGCATGAGGGGTTTACGACGTGATGGGAATGCTGAAGATGCCTGGGCCACCTCGCAGAACTCA AAATCGTATGGGAAAAGTATTTCCATAGAAACTGCCAATCTCAGAGAATATGCTAGATACGTACTGAGGA CTATCTGCCAGCAGGAATGGGTGGGAGAACACTGCTTAAAAGAACCAGAACGACTGTGTACAGACAAAGA ACTGATACTGGACCCCGTGCTTTCAAACATGCAAGCACAGAAATTGTTGCAGCTTATCTGCTATCCTCAT GGCATTAAGGAGTGTACAGAGGGGGACAACCTGCAAAGACAGCACATTAAGCGCATTCTTCAGAATCTTG AGCAGTGGACGTTGAGACAGTCCTGGCTGGAGCTCCAGCTCATGATCAAACAGTGCTTGAAGGACCCTAG CTCTGGTTCTGTGGCCGAAATGAATAATTTGCTGGACAATATCGCAAAGGCAACGATAGAAGTGTTCCAG CAATCTGCAGACCTAAATAATAATGCCTCCAATTCTGGCATGAGCCTCTTCAACCCAAACACTATTGGAA GTGTTGATCCAAGTAGCACAAGACAGAATGGAATAAAGACATTCTTAAGTTCCTCTGAACGCAGGGGTGT GTGGCTGGTGGCCCCCCTCATCGCCAGGCTGCCAACATCTGTGCAGGGAAGAGTGTTGAAAGCCGCTGGG GAAGAGCTGGAGAAGGGGCAGCACCTGGGTTCCTCTTCAAAGAAGGAAAGAGATAGGCAGAAGCAGAAAA GTATGTCTCTTTTGAGCCAGCAGCCTTTCCTCTCATTGGTCCTTACCTGCCTTAAGGGGCAAGATGAGCA ACGGGAAGGCCTTCTAACATCTCTGCAGAATCAAGTCAATCAGATTTTAAGTAACTGGAGGGAAGAAAGA TACCAAGATGATACGAAAGCCCGCCAGATGATGCATGAGGCCCTACAGCTCCGCCTGAATCTGGTAGGAG GAATGTTTGACACGGTACAGCGGAGCACGCAGGGGACCACCGACTGGGCCCTCCTACTCCTCCAGATAAT TACTTCGGGAACTGTTGACATGCACACTAACAATGAATTATTCACAACGGTTCTTGACATGCTGGGTGTT TTAATCAATGGAACCCTGGCTTCTGACCTATCAAGTGCTTCCCCCGGGGGATCAGAAGAGAACAAACGTG CATACATGAACTTAGTAAAGAAACTGAAAAAAGAACTAGGAGACAAGAGGTCAGAAAGCATTGACAAAGT TCGACAGTTGCTGCCTTTGCCAAAGCAGACCTGCGATGTCATCACGTGTGAACCCATGGGTTCTTTGATC GATACAAAGGGAAACAAAATTGCTGGATTTGACTCTATTGATAAAAAACAGGGTCTCCAGGTCTCTACGA AGCAGAAAGTGTCCCCATGGGACCTGTTTGAGGGTCAGAAGAACCCAGCCCCGCTGTCCTGGGCCTGGTT CGGCACTGTCCGTGTGGACCGGAAGGTGATCAAGTATGAGGAGCAGCAGCACTTCCTTCTGTACCACACG CACACCATGCCCAAGCCCCGAAGCTACTACCTCGAGCCGCTGCCCCTACCTCCTGAGGAGGAAGAGGAAG AACTCACGTCTCCAGTTTCTCAGGAACCAGAAAGGAAATCAGCTGAGCTGTCAGATCAAGGAAAAGCTAC AGCTGATGAAGAGAAGAAGACAAAGGGAAGGAAGCGTAAGACAAAATCGAGCTCACGCATCGATGAGTAT CAACAGACCAACTTATACCGAGTGCCTCCCAATTACTCCCCCATGTCCTCCCAAATGACGCATCATCCGC AACCTGCACTGTGGGGTTACAACCTGGTGAGCCAGCCTCAGCAGCCTAGCTTTTTCCTCCAGAATCCGTC TCTGAATCCAGGTGGCTCCCGATTGGACCCTGCGGGCTCCTTTGTCCCAACCAATACCAAGCAAGCTTTG TCCAACATGCTGCAGAGACGCTCAGGCGCCATGCTGCAGCCACCCTCCCTTCATGCAGTCACGTCTCAGC AACAGCTGCTACAGATGAAGCTTCTGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA GCGGCTTCTCAGGCAGGCCCAGACTCGACCTTTCCAACAGGGCCAGCCAGGGGACCAGGCTGCTCTCTTT ACTGCACAAGCACGGCCCTCCCCTCAGCTCCCTCAGTATCCAGGGCTGCAACAAGCACAGACCATGCCCC AGGGCTACACAATGTATGGAACACAGATGCCTTTGCAACAGGCAGCTCAGCAGCAGCCTGGTGGCGTGGT CCTTTCCCCCAGCTACAACTCCAGGGCCTATCCGGCTGCACATTCCAGCCCAGCGCTGATGGAAAGACTC CGGCAGCTTCAGCAGCAGCCAAGTGGCTACGTGCAGCAGCAGGCCTCCCCGTACCTGCAGCCCGTAGCTG GCTCTCAGAGACTGAACCACCAGGCTCTACAGCAGAGCCCTCTGGTGGGCGGGGGAATTGACGCTGTGCT GACTCCCGCACATCCCAACCTCCCCTCGGTGCCCCTGCCTCAGGACCCAATGAGGCCCAGACAGCAGCAA GTTCGACAGCAGCAGAGACTCCTCCAGATGCAGCAGCCCCAGCAGGCCCCCCAGCCCCAGCAGCCCTCAC AGACCCAGAGTCAGGCCCTTGGTCTCCAAGCAATGCAGCCCCAGCAGCCTTTGTTCCCCAGGCAAGGCTT GCAGCAGACCCAGCAACAGCAACAGACAGCCGCCCTGGTGCGACAGCTCCAAAAGCAGCTTTCTAGTAAC CAGCCACAGCAAGGAGTGACTCCCTGTGCACACCCTTCACACTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177855
Insert Size	6558 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177855.3, NP_808523.2
RefSeq Size	10275 bp
RefSeq ORF	6558 bp
Locus ID	329650
UniProt ID	Q8BQM9
Gene Summary	May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]

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