Cacna1d (NM_001083616) Mouse Untagged Clone

CAT#: MC225197

Cacna1d (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 2, (10ug)



  "NM_001083616" in other vectors (2)

CNY 34,490.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225197 representing NM_001083616
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGATGATGATGATGATGAAAAAAATGCAACATCAACGGCAGCACCAAGAGGACCATGCGAACGAGG
CAAACTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAA
GCAAACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGC
ACCTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC
AGGGTAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAG
AGCCTGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT
GTGGCCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG
TAGAATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCT
GCATCCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTT
AGTGTAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCT
TTGATGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTT
ACAAGTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTT
GTAATTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTG
CTGACTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCAC
CGCCAATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTT
GCCTTTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTA
ATGATGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT
CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGA
GATTTCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCC
AGGCAGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCC
CACCAGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGA
ACTCTCTGTCAGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCA
GGAGGTGCAGGGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACAC
TTTAACTATTTCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAA
GTCCTCTTGGCTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTG
TCTCCCTCTTCAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACT
GGAGCTCATGTCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACC
AGGCACTGGACCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGC
TGTTACTCCTCTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAA
TTTTGACGAGACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAG
ATCCTGACAGGTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTT
CCGGGATGATTGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTT
CTTGGCCATCGCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAA
GAAAAAGAAAGGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCA
ACCAGATAGCCAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCC
TTACCCGCCCTGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCT
GCTGGTCCCCGCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAA
GCGCCTTCTTCATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACAT
CTTCACCAACCTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGT
AGCCACTCATTCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAA
TCCTGTTAAAGATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTT
GCTGGACATGCTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTG
AAGATTCTGAGGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACG
TGGTCCAGTGTGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTT
CATGTTTGCATGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGT
AACCCTGAGGAGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTG
AGAGGATCTGGCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGT
CTCAACTTTTGAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCT
GTCTACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGA
TGAACATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGA
GCTGGACAAAAATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCC
AAAAACCCATACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCC
TCATCATGCTCAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCAT
GGACATTCTGAACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAG
CCCAAGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACG
TGGCCCTCAGCGAAGCCGACAACTCTGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCG
AGTGATGCGGTTGGTGAAACTTCTCAGCAGGGGGGAAGGCATCCGGACACTGCTGTGGACCTTCATCAAG
TCCTTTCAGGCGCTGCCGTATGTTGCCCTCCTCATTGCCATGCTCTTCTTCATCTACGCTGTCATAGGCA
TGCAGATGTTTGGGAAAGTTGCCATGAGAGATAACAACCAGATCAATAGGAACAACAACTTCCAGACCTT
TCCTCAGGCAGTGCTCCTACTCTTCAGGTGTGCAACAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGC
CTCCCAGGAAAGCTGTGCGACCCAGACTCCGATTACAACCCAGGAGAGGAGTACACTTGTGGGAGCAACT
TTGCCATTGTCTACTTCATCAGCTTTTACATGCTCTGTGCATTCCTGATCATCAACCTCTTTGTGGCTGT
CATCATGGACAATTTTGACTATCTGACTCGGGACTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTC
AAAAGAATATGGTCAGAATATGACCCTGAAGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGC
TCCGGCGGATCCAGCCTCCCCTGGGGTTTGGAAAATTATGCCCACACCGAGTAGCATGTAAGAGATTGGT
TGCCATGAACATGCCTCTCAACAGTGATGGGACAGTCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGG
ACAGCTCTCAAGATCAAGACTGAAGGGAACCTGGAGCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGA
AAATCTGGAAGAAGACAAGCATGAAGCTGCTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAAC
CGTGGGGAAGTTCTATGCCACTTTCCTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAA
GGACTGGTGGGGAAGTACCCTGCGAAGAACACCACGATTGCCCTACAGGCGGGATTAAGGACCCTGCATG
ACATTGGGCCAGAAATCCGACGGGCTATATCCTGTGACTTGCAAGATGACGAGCCAGAAGACTCCAAACC
AGAAGAAGAAGATGTATTCAAAAGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGAT
AGGAGAGATTCCCTTCAGCAGACCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCAC
CTGCAAGTGATACTGAGAAACCGCTGTTTCCTCCAGCAGGAAATTCGGGGTGTCATAACCATCATAACCA
TAATTCCATAGGGAAGCAAGCTCCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCT
GCCCACGGAAAGCCGCCCAGCATTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATTACTCCTGCAAGC
ATGATCGAGAGCTTCAAAGAAGGTCCAGTATTAAAAGAACCCGCTATTACGAGACTTACATTAGGTCTGA
GTCAGGAGATGAGCAATTTCCAACCATTTGCCGTGAAGATCCTGAGATACACGGCTACTTCCGAGACCCC
CGCTGCTTGGGGGAACAGGAGTATTTCAGTAGCGAGGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGA
GCAGGCAAAACTACAACTACTACAACAGGTACCCAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTA
CCACCACCCCCAAGGCTTCTTAGAAGATGATGACTCTCCCACTGGCTATGATTCACGGAGATCTCCGAGG
AGACGTCTTCTACCTCCTACCCCACCATCCCATCGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGC
AAAGCAGCCAAGACGATGTCCTTCCATCGCCTGCCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGAT
GCAGCAGCAGATCATGGCCGTTGCAGGCCTAGATTCCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCT
ACCCGGTCGTGGGCCACCCCTCCAGCAACCCCTCCATACCGAGACTGGAGCCCATGCTACACCCCTTTGA
TCCAGGTGGACCGCTCAGAATCTATGGACCAGGTCAATGGCAGCCTGCCATCCCTACACCGAAGCTCCTG
GTACACAGATGAACCCGACATCTCCTATAGGACTTTCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTC
CGGAACAAGAACAGCGACAAGCAGAGGAGTGCAGACAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCT
TGGGACGCTACGCGAGGGACCCCAAATTTGTCTCAGCGACAAAACATGAGATTGCCGATGCCTGTGACCT
AACCATAGACGAGATGGAAAGTGCTGCCAGCACCCTGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGG
GACATGGGCCCCATCTCCCACCGCCAGGACTATGAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGG
AGCCAGACCCTGGGCGAGAGGAAGAGGACCTGGCAGATGAGATGATTTGCATTACCACCTTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001083616
Insert Size 6435 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001083616.2, NP_001077085.1
RefSeq Size 9075 bp
RefSeq ORF 6435 bp
Locus ID 12289
UniProt ID Q99246
Gene Summary This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]
Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region, compared to variant 3. This results in a shorter protein (isoform 2), compared to isoform 3.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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