Cacna1c (NM_001159535) Mouse Untagged Clone

CAT#: MC225195

Cacna1c (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1C subunit (Cacna1c), transcript variant 4, (10ug)



  "NM_001159535" in other vectors (2)

CNY 34,390.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Cav1.2; Cchl1a1; D930026N18Rik; MBC; MELC-CC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225195 representing NM_001159535
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAATGAAAACACGAGGATGTACGTTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC
GCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCTCCCGAGCACATCCCTACTCCAGG
GGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGCTCATGGGCAGTGCTGGCAAC
GCAACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGGGAAACCCAAGAAGCAGGGGG
GCACAACCGCCACACGGCCGCCCCGGGCTCTGCTGTGTCTGACCCTGAAGAACCCTATCCGGAGGGCGTG
CATAAGCATTGTTGAATGGAAACCATTTGAAATCATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC
TTAGCAATCTATATTCCCTTTCCGGAAGACGACTCCAACGCCACCAACTCCAACCTGGAACGAGTGGAGT
ATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTAATTGCCTACGGACTTCTCTTCCACCC
CAACGCTTACCTCCGCAATGGTTGGAATTTACTGGATTTTATAATCGTGGTTGTAGGGCTTTTTAGTGCA
ATTTTAGAACAAGCAACCAAAGCTGATGGGGCCAATGCTCTAGGAGGGAAAGGAGCTGGATTCGACGTGA
AGGCACTGAGAGCGTTCCGCGTGCTCCGTCCACTGCGGCTAGTGTCCGGAGTCCCAAGTCTCCAGGTGGT
CCTGAACTCCATCATCAAGGCCATGGTGCCTCTGCTGCACATTGCCCTTCTTGTGCTCTTCGTCATCATC
ATTTATGCTATTATCGGCCTGGAGCTCTTCATGGGAAAGATGCACAAGACCTGCTACAACCAGGAGGGCA
TAATAGATGTTCCGGCAGAAGAGGATCCTTCCCCTTGTGCTTTGGAGACAGGCCATGGGCGACAGTGTCA
GAACGGGACCGTGTGCAAACCCGGGTGGGATGGGCCCAAGCACGGCATCACCAACTTCGACAACTTCGCC
TTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGACGTGCTGTACTGGATGCAAG
ACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATCTTTGGATCCTTTTTCGTTCT
AAATCTGGTTCTCGGTGTTTTGAGCGGGGAGTTTTCCAAAGAGAGGGAGAAAGCCAAAGCCCGAGGAGAT
TTCCAGAAGCTTCGAGAGAAGCAGCAACTAGAAGAAGATCTCAAAGGCTACCTGGACTGGATCACCCAGG
CAGAAGACATTGACCCCGAGAATGAGGACGAGGGCATGGATGAAGACAAGCCTCGAAACATGAGCATGCC
CACAAGTGAGACTGAGTCTGTCAACACTGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGTGGA
GCCAGGCTTGCCCATCGGATCTCCAAATCCAAATTCAGCCGCTACTGGCGCAGGTGGAATCGATTCTGCA
GAAGAAAATGCCGTGCAGCAGTTAAGTCCAACGTCTTCTACTGGCTCGTGATCTTCCTGGTGTTCCTCAA
CACCCTCACCATTGCCTCCGAACATTACAACCAGCCTCACTGGCTCACAGAAGTGCAAGACACAGCCAAT
AAAGCCCTCCTGGCCCTTTTCACTGCAGAAATGCTCCTGAAGATGTACAGCCTGGGTCTTCAGGCCTATT
TTGTGTCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGTGGGGGCATCCTGGAGACCATCCTGGTGGA
GACGAAGATCATGTCTCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGTTGCTCAGGATCTTCAAGATC
ACCAGGTACTGGAATTCCTTGAGCAACCTTGTGGCATCCTTGCTGAACTCAGTGCGCTCCATTGCCTCCC
TGCTGCTGCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGGAAGTT
CAATTTCGATGAGATGCAGACCCGTAGGAGCACGTTCGATAACTTCCCGCAGTCTCTCCTCACTGTGTTT
CAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTT
TTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAATTATATCCTACTGAATGT
GTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCTCAGCCCAAAAGGAGGAGGAA
GAAGAGAAGGAGAGGAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAAACAGGAGGTGATGGAGAAGC
CAGCCGTGGAGGAGAGCAAAGAGGAGAAAATTGAACTGAAATCCATTACAGCCGATGGAGAATCCCCACC
CACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAACGAGGATAAGAGTCCCCACTCCAACCCA
GACACTGCAGGGGAAGAGGATGAAGAGGAGCCAGAGATGCCTGTGGGGCCACGCCCCCGGCCCCTGTCTG
AGCTGCACCTTAAGGAAAAGGCAGTTCCCATGCCGGAAGCCAGTGCATTTTTCATCTTCAGCCCAAACAA
CAGGTTCCGCCTGCAGTGCCACCGTATTGTCAATGACACGATCTTCACCAACCTCATCCTCTTCTTCATT
CTGCTCAGCAGCATCTCTCTGGCTGCTGAGGACCCCGTCCAGCACACCTCCTTCAGGAACCATATCCTAG
GCAATGCAGACTATGTCTTCACTAGTATCTTTACATTAGAAATTATCCTTAAGATGACTGCTTACGGGGC
TTTCCTGCACAAGGGCTCTTTCTGCCGAAACTACTTCAATATCCTGGACCTGCTGGTGGTTAGCGTGTCC
CTCATCTCCTTTGGCATCCAGTCCAGCGCGATCAACGTTGTGAAGATTTTACGAGTGCTGCGAGTCCTCA
GACCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGCGTGTTTGTGGCCATCCG
GACCATCGGGAACATCGTAATTGTCACCACTCTGCTGCAGTTCATGTTCGCCTGCATTGGGGTCCAGCTC
TTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCTAAACAGACGGAGGCAGAATGCAAGGGTAACTATA
TCACATACAAAGATGGAGAGGTCGATCACCCCATTATCCAGCCTCGAAGCTGGGAGAACAGCAAGTTTGA
CTTTGACAATGTTTTGGCAGCCATGATGGCTCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTG
CTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCA
TCTTCTTCATCATCTATATCATCATCATTGCCTTCTTCATGATGAACATCTTCGTGGGTTTCGTCATTGT
CACCTTCCAGGAGCAGGGGGAACAAGAGTACAAGAACTGTGAGCTGGACAAGAACCAGAGACAATGTGTG
GAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGT
ACGTGGTCAACTCTACCTACTTCGAGTATCTGATGTTCGTTCTCATCCTGCTCAACACCATCTGCCTGGC
CATGCAGCACTATGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATACTCAACATGCTTTTCACCGGC
CTCTTCACAGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGCACTATTTCTGTGATGCATGGA
ATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAATCACCGAGCCAGCTGAACATAC
CCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCGCATCTCCATCACCTTCTTCCGCCTCTTCCGG
GTCATGCGCCTGGTGAAGCTGCTGAGCCGCGGGGAAGGCATCCGAACCCTGCTGTGGACCTTCATCAAGT
CCTTCCAGGCTCTGCCCTATGTGGCTCTTTTGATTGTGATGCTGTTCTTTATCTATGCAGTGATTGGGAT
GCAGGTGTTTGGGAAGATTGCCCTGAATGACACCACAGAGATCAATCGGAACAACAACTTCCAGACGTTC
CCCCAGGCTGTGTTACTGCTGTTCAGGTGTGCCACCGGAGAGGCCTGGCAGGACATCATGCTGGCCTGCA
TGCCAGGCAAGAAGTGTGCCCCAGAGTCTGAGCCCAGCAACAGCACGGAAGGGGAGACCCCCTGTGGCAG
CAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTCTTTGTA
GCTGTTATCATGGACAACTTTGACTACCTGACTAGGGATTGGTCTATCCTCGGTCCCCATCACCTGGATG
AATTCAAGAGAATCTGGGCCGAGTATGACCCTGAAGCCAAGGGTCGGATCAAACACTTGGATGTGGTGAC
CCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAAATTGTGTCCTCACCGTGTGGCCTGCAAACGC
CTGGTGTCCATGAACATGCCTCTGAACAGCGATGGCACAGTCATGTTCAATGCTACCCTGTTTGCCCTCG
TCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAGAGCAAGCCAATGAGGAGCTTCGGGCCATCAT
CAAGAAAATCTGGAAGAGGACTAGCATGAAGCTGTTGGACCAGGTGGTGCCCCCTGCAGGCGATGACGAG
GTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAAGAGTACTTCAGGAAATTCAAGAAGCGAAAAG
AGCAGGGGCTGGTGGGCAAGCCCTCACAAAGGAATGCACTGTCCCTCCAGGCTGGCTTGCGCACCTTGCA
TGACATTGGGCCTGAGATCCGGCGGGCCATCTCTGGGGATCTGACTGCTGAGGAGGAGTTGGACAAGGCT
ATGAAGGAGGCGGTGTCTGCTGCCTCCGAAGATGACATCTTCAGGAGGGCTGGAGGCCTGTTCGGCAACC
ATGTCACCTACTATCAGAGTGACAGCAGGGGCAACTTTCCTCAGACGTTCGCCACCCAGCGCCCACTGCA
CATCAACAAGACAGGGAACAACCAAGCTGACACTGAGTCACCGTCCCATGAGAAGCTGGTGGACTCCACG
TTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCCAACATCAACAATGCCAACAACACTGCCCTGG
GCCGCTTCCCCCATCCCGCTGGCTACTCCAGCACGGTCAGCACTGTGGAGGGCCATGGGCCTCCCTTGTC
CCCTGCTGTCCGAGTACAGGAGGCAGCATGGAAGCTCAGCTCTAAGAGGTGCCACTCCCGAGAGAGCCAG
GGAGCCACGGTGAATCAGGAGATATTTCCAGATGAGACCCGCAGCGTAAGGATGAGTGAAGAAGCCGAGT
ACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATGTTCTCCTACCAGGAAGATGAACACCGACAACTGAC
CTGCCCAGAGGAGGACAAGAGGGAGATCCAGCCATCTCCAAAGAGGAGTTTCCTTCGCTCTGCCTCTCTA
GGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGACAAAAGGATCAAGGGGGAGACATCTCTCAGA
AGACAGCCTTGCCCTTGCATCTGGTTCATCATCAGGCATTGGCAGTGGCAGGCTTGAGCCCCCTCCTGCA
GAGAAGCCATTCTCCTACCACATTCCCCAGGCCGTGCCCCACACCCCCTGTCACTCCAGGCAGCCGGGGC
AGACCCCTACGGCCCATCCCTACCCTACGGCTGGAGGGGGCAGAGTCCAGCGAGAAACTCAACAGCAGCT
TCCCATCCATCCACTGCAGCTCCTGGTCTGAGGAGACGACAGCCTGTAGTGGGAGCAGCAGCATGGCCCG
GAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGCTGGAGCTCCAGGGAGACAGTTCCATGGCAGT
GCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACTGGGACAGTTTGCTCAAGATCCCAAGTTCA
TCGAGGTCACCACCCAGGAGCTGGCTGACGCCTGCGACATGACAATAGAGGAGATGGAGAACGCCGCAGA
CAACATCCTCAGTGGGGGCGCCCAGCAGAGCCCCAACGGCACCCTCTTACCTTTTGTGAACTGCAGGGAC
CCGGGGCAGGACAGGGCTGTGGCCCCAGAGGACGAGAGCTGCGCATATGCCCTGGGGCGAGGCCGGAGCG
AGGAGGCGCTCGCGGACAGCAGGTCCTACGTCAGCAACCTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001159535
Insert Size 6414 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001159535.2, NP_001153007.1
RefSeq Size 13586 bp
RefSeq ORF 6414 bp
Locus ID 12288
Gene Summary Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:14609949, PubMed:18586882, PubMed:21216955, PubMed:25368181, PubMed:28119464). Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm (By similarity). Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm (PubMed:21216955). Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells (PubMed:14609949, PubMed:28119464). Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (4) has an alternate 5' exon and an alternate internal exon in the CDS, as compared to variant 1. The resulting isoform (4) has a shorter and different N-terminus and a shorter and different internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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