Espl1 (NM_001014976) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL024103; AU045071; Cerp; ESP1; PRCE; SSE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225179 representing NM_001014976 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAACTTCAAAGGAGTCAACTTCGCCACTCTGCTCTGCAGCAAGGAGGAGACCCAGCAGCTGCTGC CTGACTTGAAGGAGTTCCTGTCCAGATCCCGGACTGATTTCCCCAGCAGCCGAACAGATGCCGAAAGAAG ACAAATTTGCGATACCATCCTGAGGGCTTGCACCCAGCAGTTGACTGCCAAGCTCGATTGCCCTGGGCAC CTGAGGAGTATTTTGGACCTGGCAGAGCTGGCCTGTGATGGCTATTTGTTGTCTACTCCACAGCGCCCTC CCCTCTACCTGGAACGCATTCTCTTCATCCTACTTCGCAATGGTTCCACTCAGGGAAGCCCAGACACTGT GCTGCGCCTTGCCCAGCCTCTCCATGCCTGCTTGGTTCAGAACTCTGGAGAGGCTGCCCCTCAGGACTAT GAGGCCGTGACCCGGGGCAGCTTCTCTCTGTTTTGGAAGGGAGCAGAGGCACTGTTGGAGCGGCGAGCTG CGTTCTCAACTCGGCTGAATGCCTTGAGCTTCTTGGTGCTCTTGGAGGATGGCAGTGTCCCCTGTGAGGT CCCTCACTTTGCTTCTCCAACCGCCTGTCGTTTAGTTGCCGCCTATCAGCTGTACGATGCTACAGGACAA GGTCTAGATGAAGCTGATGCCGACTTCCTGTATGAAGTGCTTTCTAGGCACCTGATCAGAGTCTTAGTAG GTGAGGGTGGTAGCTCTCCCGGTCCTCTCTCGCCTCAGAGGGCCCTCTGCCTCTTGGAAATCACCCTGGA ACACTGCCGTCGGCTCTGCTGGAACCACCACCACCGCCAGGCTGCCAGAGCAGTGGAGAGGGCTCGTAAT CACCTAGAGAAGACCAGTGTAGCTCCTAGCCTCCAGTTATGCCAGATGGGGGTTGAGCTGTTGGAAGCTG TAGAAGAAAGACCAGGGGCAGTGGCCCAGCTTCTGAGGAAGGCAGCAGCCGTTCTTATCAACAGTATCGA GGCGCCGTCGCCCCCGCTCCGGGCATTGTACGACAGCTGCCAGTTCTTCCTTTCAGGCCTGGAGAGAGGC ATCAGGAGGCACTGTGGACTTGATGCCATATTGAGTCTCTTTGCTTTTCTTGGGGGATACAGCTCCCTTG TCCGGCACCTGAGAGAAGTCTCTGAAGCATCTTCCAAGCAGCAGCAGTGCTTGCTTCAGATGCACTTCCA GGGTTTCCACCTCTTCACCGGGATTGTGTATGACTTTGCTCAAGGCTGTCAGGCAACTGAGCTTGCTCAG CTAGTGGACGGTTGCAGATCTGCTGCTGTCTGGATGTTGGAGGCCTTAGAGGGCCTGTCGGGTGGAGAGC TGGCTGACTACCTGAGCATGACTGCCTCTTACACCAGCAACTTGGCCTACAGCTTCTTTAGTCAGAAGCT CTATGAGGAGGCCTGCGTCATCTCAGAGCCAGTCTGTCAGCACTTGGGCTCGGCAACGTCGGGTGCTTGT CCTGAAGTGCCTCCTGAGAAGCTACATAGGTGCTTCAGGCTGCATGTGGAGAGTTTGAAGAAACTGGGTA AACAGGCCCAGGGCTGCAAGATGGTGACCTTATGGCTGGCAGCTCTGAAACCCTACAGTCTGGAACACAT GGTCGAGCCAGTCACTTTCTGGGTTCGAGTCAAAATGGATGCATCCAGGGCAGGAGACAAGGAGCTACAA CTTCAGACTTTGCGAGACAGCCTAAGCTGCTGGGACCCAGAGACACAGTCTCTGCTTCTCAGGGAGGAGC TGCGGGCCTACAAGTCAGTACGGGCTGACACTGGGCAGGAGCGCTTCAACATCATCTGTGATCTGCTCGA GCTGAGCCCTGAAGAGACAGCAGCTGGAGCCTGGGCACGAGCTACCTACCTGGTGGAACTGGCCCAAGTG CTGTGCTACCACAACTTTACCCAGCAGACCAACTGCTCTGCCTTGGATGCTGTCCAGGAAGCCCTGCAGC TTCTGGAGTCCGTGAGTCCTGAGGCCCAGGAGCAGGATCGACTTCTGGATGATAAAGCACAGGCCTTGTT ATGGCTTTACATCTGTACCTTAGAGGCCAAGATGCAGGAAGGTATTGAGCGGGATCGGAGAGCCCAGGCC CCTAGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTGCAGGAAGATCGTTTTC TGTATAGTAGCATTGCCTTCAATCTGGCTGCTGATGCAGCTCAGTCCAAATGCCTGGACCAGGCCCTGAC TCTGTGGAAAGAAGTGCTCACCAAGGGGCGAGCCCCAGCTGTGCGGTGTCTCCAGCAGACTGCAGCCTCC CTGCAAATCCTAGCGGCCGTCTATCAGTTGGTGGCAAAGCCCTTGCAGGCTCTGGAGACCCTCCTGCTCC TGCAGATTGTATCTAAGAGACTGCAGGACCACGCAAAGGCAGCCAGCTCTTCCTGCCAGCTTACCCAGCT ACTCTTGAACCTTGGCTGTCCCAGCTATGCGCAGTTGTATCTGGAAGAGGCGGAGTCAAGCCTGAGGAGC CTGGACCAGACAAGTGACGCGTGCCAGCTGCTGTCCCTGACCTGTGCTCTGCTTGGAAGTCAGCTCTGCT GGGCTTGCCAGAAGGTAACTGCAGGCGTTTCTTTGTTGCTTTCTGTGCTTCGGGATCCTGCCCTCCAGAA GTCATCTAAGGCTTGGTACTTGCTACGTGTCCAGGCCCTGCAAGTTCTGGCTTTTTACCTCAGTCTCTCG TCCAACCTCCTCTCAAGTGCCCTGCGTGAGCAGCTCTGGGATCAAGGCTGGCAGACCCCGGAGACGGCGC TGATTGATGCCCACAAGCTCCTCAGAAGCATCATCATTCTGCTCATGGGAAGTGATGTGCTCTCCATTCA GAAAGCAGCCACCGAGTCGCCCTTTCTGGACTACGGTGAGAATCTGGTGCAAAAATGGCAGGTTCTCACA GAGGTGCTGACCTGCTCGGAGAGGCTGGTCGGCCGCCTGGGTCGCCTGGGGAACGTGAGTGAGGCCAAGG CCTTTTGCTTGGAGGCCCTAAAACTTACGACTAAGCTGCAGATACCTCGACAGTGTGCCCTGTTCCTTGT ACTGAAGGGTGAGCTGGAGCTGGCCCGGGGTGACATTGACCTCTGTCAGTCAGACTTGCAGCAGGTTCTC TTCTTGCTCGAGTCTTCCACAGAGTTTGGTGTTGTGACCCAGCATCCAGACTCAGTGAAGAAGGTGCACA CGCAGAAAGGGAAGCATAAGGCTCAAGGCCCATGTTTCCCACCCCTGTCAGAGGAGGAGCCCTTCCTGAA AGGTCCTGCCCTTGAGCTGGTGGACACTGTGCTCAACGAACCTGGTCCCATCCAGTCCTCTGTAAACTCC TCCCCAGTCCTGAAAACCAAGCCACCACCCAACCCTGGCTTCCTGTCTCACTTGCCCAGCTGTGACTGCT TACTGTGTGCCAGCCCTGCCCTCTCAGCAGTCTGTCTGCGCTGGGTGCTGGTCACTGCCGGAGTGAGGCT GGCCACCGGTCACAAAGCTCAGGGTCTGGATCTGCTGCAAGCTGTGCTGACGCGTTGCCCTGCAGCCACC AAGCGCTTCACACAGAGTCTCCAAGCTTCTCTGAATCACAGAACAACCCCCTCTTGTGTTCCCAGCCTCT TTGATGAGATCATGGCTCAAGTGTACACGCACTTGGCATTGGAGTTCCTGAATCAGACATCTGAAAAGAG CCTGGGGAAGGTCCTGGCGTCGGGGCTGAAGTTTGTGGCAACACGGATACAGAGCTTGGAGATCTGGAGA GCCCACCTGCTCTTGGTTCAGGCCCTTGCAAAGCTGGCCCACTTCAGCTGCTGTACTTCCGAACTCTTTG CAAGCTCCTGGGGCTGGCACCCACCATTAGTAAAAAGTCTGCCAGTCTTAGAGCCTGCTAAAATTCGGAG ACAAAAATGCTCTGGACGGGGCCGCCGGCGGATAGCCTCTGTTCCCCCACCCCTCCATAACAGCTCTCAG AAAGGTCTGGAAGAGGAAGGGCCACCTTGTACCCCTAAGCCTCCAGGTCGGGCTAGGCAAGCTGGCCCTC GTGTCCCTTTCACCATATTTGAAGAAGTCCACCCCACCAAGAGCAAGCTCCAGGTTCCCTTGGCTCCCAG GGTCCACAGGCGGGCCCAGACTCGCCTCAAGGTGATCTTCAGTGATGACAGTGACCTGGAAGACCTTGTC TCAGCTGACACACAACTTGTAGAGGAGCCTAAGAGGCGAGGGACTGCTTCCCGTACCCGGGGCCAAACTA GGAAGGGACGTAGCTTAAAGACAGATGCGGTGGTTGCTATAGAAAGTACCCCTGGACATTCCAGTGTCAG TGGGAGGACTCGGAGGGCCAGGAAGGTGGCTTCAAGAAATTGTGAAGAAGAGAGTCCCAAAGCACCCCTC TGTGTCTGGGCCAGCCAGGGACCCGAGATCATGAGAAGTATCCCTGAAGAGGAGCCAGTGGACAACCATC TGGAAAAAAGCTTCGAGATTCTCAGGGGTTCTGATGGGGAAGACTCGGCCTCAGGTGAGAAGGCGGCAGC TGCAGACACTGGTCTACCCGTAGGAGAGTGTGAGGTGTTGAGACGGGACTCCAGCAAGGCAGAGAGACCT GTCTTGTACTCAGACACGGAGGCCAATAGTGACCCTAGTCCTTGGCTCCCACCCTTCTCAGTTCCTGCAC CCATTGATCTTTCTACCCTGGATTCCATTTCTGACTCATTGAGCATTGCTTTCCGTGGTGTTAGTCACTG CCCTCCAAGTGGGCTCTATGCTCACCTCTGCCGCTTCTTGGCCCTGTGTCTGGGCCACCGAGATCCTTAT GCCACTGCGTTCCTTGTTGCTGAGTCTATCTCCATCACCTGCCGTCACCAGCTGCTCACCCACTTGCACC GACAGCTCAGCAAGGCCCAGAAGCAGCAAGAATCACCTGAACTGGCAGAGCATCTGCAGAGGCTGGACCT GAAGGAGAGGCCCGGAGGTGTCCCACTGGCCCGTATCCAGCGCCTCTTTTCCTTTAAGGCTTTGGGATCT GGCTGCTTCCCCCAGGCAGAGAAGGAGAGTTTCCAGGAGCGCCTGGCTCTGATCCCTAGTGGGGTGACTG TCTGTGTGTTGGCTCTGGCCACCCTCCAGCCTGGAACCTTGAGCAACACTCTTTTACTGACCCGCCTGGA AAAGGACAACCCCCCAATTACTGTGAAGATCCCCACTGCCCAAAATAAGCTCCCTCTAAGCGCAGTGCTG AAAGAGTTTGATGCCATCCAGAAGGACCAAAAAGAAAACAGCAGCTGTACTGAGAAACGAGTATGGTGGA CGGGCCGGCTGGCACTGGACCAAAGGATGGAGGCACTCATCACCGCCCTGGAGGAACAGGTGCTGGGCTG CTGGAGGGGGCTGCTGCTGCCATGCAGTGCTGACCCCAGCCTTGCCCAGGAGGCCTCCAAACTACAAGAG CTGCTGCGGGAATGTGGCTGGGAATATCCTGACTCCACTCTCCTGAAAGTCATCCTTAGTGGGGCCAGAA TCCTCACCAGCCAGGATGTTCAGGCCTTGGCGTGTGGGCTGTGCCCAGCCCAGCCTGACCGAGCCCAAGT GCTCCTGAGCGAGGCAGTAGGACAGGTTCAGAGCCAGGAGGCCCCTCGAAGTCAACATCTTGTATTGGTG TTAGACAAGGACCTGCAAAAGCTGCCTTGGGAAAGCACGCCCATCCTCCAAGCACAGCCGGTCACCCGGC TGCCCTCCTTCCGCTTCCTGCTCAGCTACACAGTCACTAAAGAGGCTGGAGCCTCATCAGTGCTGAGCCA AGGTGTTGATCCACAGAATACCTTCTATGTGCTAAACCCTCACAGTAACCTGTCGAGCACAGAGGAGAGA TTTCGAGCCAGTTTTAGCAGTGAAACTGGCTGGAAAGGAGTGATTGGGGAAGTGCCAAGCCTCGATCAGG TGCAGGCTGCCCTGACAGAGCGGGATTTATATATCTACGCAGGGCACGGAGCCGGTGCCCGCTTCCTTGA TGGGCAGGCTGTCCTGCGGCTGAGCTGCCGGGCGGTGGCCCTGCTGTTCGGCTGCAGCAGTGCAGCCCTT GCCGTGCATGGAAACCTGGAGGGAGCTGGTATCGTGCTCAAGTACATCATGGCTGGCTGCCCCTTGTTTC TAGGTAATCTCTGGGATGTGACTGACCGTGACATTGACCGTTACACAGAGGCTTTGCTGCAGGGCTGGCT GGGAGCAGGCCCAGGGGCTCCCTTTCTCTACTATGCCAGCCAAGCCCGCCAGGCCCCTCGACTCAAGTAC CTCATTGGTGCTGCACCTGTAGCCTACGGCCTACCTATCTCTCTGCAGACACCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001014976
Insert Size	6357 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001014976.2, NP_001014976.1
RefSeq Size	6630 bp
RefSeq ORF	6357 bp
Locus ID	105988
UniProt ID	P60330
Gene Summary	Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.