Numa1 (NM_133947) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6720401E04Rik; AA764025; AL022610; AU014979; NuMA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225164 representing NM_133947 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCATGCCACCCGGGCAGCTACGCTCCTTTCTTGGGTGAACAGTCTGCATGTTGCTGACCCCG TGGAGACAGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATTATTAACACCATCCATGACACTAA AGAGGGGCAACAAATCCTACAGCAGCCACTACCGGAAAGACTAGACTTTGTTTGCAGTTTTCTACAGAAA AATCGGAAACATCCTTCATCTACACAATGCCTGGTATCTGTGCAGAAAGTGATAGAGGGATCCGAGATGG AACTGGCCAAGATGATAATGCTGTTTTTATATCAGTCCACCATGAGCTCCAGAAACCTCCGGGACTGGGA GCAGTTTGAATATGGGGTTCAGGCTGAGTTAGCGGTCATCCTGAAGTTTATGCTGGATCATGAGGAGTCG CTAAACCTTACTGAGGACTTAGAGAGTTTCTTGGAGAAAGTTCCTTACACCCATGCCAGCACCCTCTCAG AAGAGCTTTCTCCACCCAGCCACCAGACCAAGAGGAAGATTCGCTTCCTAGAAATACAGAGGATTGCTTC ATCTTCCAGTGAGAACAACTTCCTCTCAGGCTCTCCATCCTCCCCTATGGGCGACATACTGCAGACCCCA CAGTTCCAGATGAGACGACTGAAGAAGCAGCTGGCAGACGAGAGGAGCAACAGAGATGACCTGGAGCTGG AGCTGTCTGAGAGCCTCAAGCTCCTCACTGAGAAGGACGCGCAGATCGCCATGATGCAGCAGCGCATTGA TCACCTGGCTCTGCTGAATGAGAAGCAGGCAGCCAGCTCCCAGGAGCCCAGTGAGCTTGAGGAGCTCCGA GGCAAGAATGAAAGCCTCACTGTGCGGCTGCATGAGACTTTGAAGCAGTGTCAGAACCTGAAGACCGAGA AGAGCCAGATGGATCGAAAGATTAGCCAGCTTTCTGAGGAGAATGGGGACCTTTCCTTTAAGGTTCGAGA GTTTGCAAACCACTTGCAACAACTTCAGGGTGCATTCAACGACCTGATAGAGGAGCACAGCAAGGCTTCT CAGGAGTGGGCAGAGAAGCAGGCTCGTCTGGAAAATGAGCTCAGCACAGCCCTCCAGGATAAGAAATGTC TTGAAGAGAAGAATGAAATTCTTCAGGGAAAGCTTTCCCAGCTGGAAGATCAAGCAACTCGGCTGCAGGA GAGCCCAGCCCCGGAGAAGGGTGAGGTGCTAGGAGACGCCTTGCAGCTGGACACTCTAAAGCAAGAGGCA GCCAAACTTGCCACAGACAACACCCAGCTCCAAACCCGCGTAGAGACACTGGAGTGTGAACGTGGCAAGC AGGAAGCCCAGCTGCTTGCTGAACGGAGCCGCTTTGAAGACGAAAAGCAGCAGCTGGCCAGCTTGATTGC TGACCTTCAGAGCTCCGTCTCTAACCTCAGCCAGGCCAAAGAGGAGTTAGAGCAAGCCTCCCAGGCCCAA GGGGCTCAGCTCACTGCCCAGTTGACCTCTATGACAGGGCTCAATGCCACCCTGCAGCAGCGAGATCAAG AGCTGGCCAGTCTGAAAGAACAGGCCAAAAAGGAACAGGCTCAGATGCTACAGACCATGCAAGAGCAAGA ACAGGCTGCCCAGGGCCTCCGCCAGCAGGTGGAGCAGCTGAGCAGCAGCCTGAAGCTGAAGGAGCAGCAA TTAGAAGAGGCAGCCAAGGAGCAGGAGGCAACCAGACAGGATCACGCCCAGCAACTGGCCATCGTTGCTG AGGCTCGAGAGGCCTCTCTAAGGGAACGGGATACTGCTCGCCAGCAGCTAGAAACAGTGGAGAAGGAGAA GGATGCCAAGCTAGAGAGTCTGCAGCAGCAACTTCAGGCTGCCAATGATGCTAGGGACAATGCGCAGACC TCGGTCACACAGGCTCAGCAGGAAAAGGCAGAGCTGAGCCAAAAGATAGGAGAGCTTCATGCCTGCATTG AAGCTTCCCACCAGGAACAGCGTCAGGTTCAAGCTCGTGTGACAGAGCTAGAAGCCCAGCTGAAAGCTGA GCAGCAAAAAACAACTGAGCGAGAAAAGGTGGTTCAGGAGAAGGCCCAGCTCCAGGAGCAGCTCCGGGCC CTTGAGGAGTCTTTGAAGATTACCAAAGGCAGCCTTGAAGAAGAGAAACGCAGGGCTGCAGATGCCCTGA AGGAGCAGCAGTGCCGTGCCACTGAGATGGAGGCAGAGTCTCGAAGCCTGATGGAGCAGCGGGAGCGGGA ACAGAAGGAGTTAGAGCAAGAGAAGGCTGGGCGAAAGGGGCTGGAGGCCCGGATACAGCAGCTTGAAGAG GCTCATCAGGCTGAGACTGAAGCCTTGCGGCATGAGCTGGCAGAGGCCACAGCTTCCCAGCACAGAGCTG AGAGTGAGTGTGAGCGGCTCATCAGAGAGGTTGAGAGCCGGCAGAAGCGGTTTGAAGCTAGACAGCAAGA GGAGGCAAGGTACGGTGCCATGTTCCAGGAACAGCTGATGGCTTTGAAAGGGGAGAAGACTGGCCAAGAG GTCCAGGAGGAGGCAGTAGAGATCCACAGTGAAGGCCAGCCAGGCCAGCAGCAGAGTCAGCTAGCCCAGC TTCATGCCAGTCTGGCCAAAGCCATCCAGCAGGTCCAGGAGAAGGAAGTGAGAGCACAGAAGCTGGTAGA TGATCTCTCAGCTCTGCAGGAGAAGATGGCTGCCACTAACAAGGAGGTGGCCTGTCTGAAGACCTTGGTG CTCAAGGCAGGTGAGCAGCAGGAAACAGCCTCACTTGAGCTGCTCAAAGAGCCCCCGAGAGCTGCAAACA GAGCGTCTGACCAGCTAGGAGAACAGCAGGGACGGCCATTCTCTAGTACACACGCAGCAGTAAAAGCTAT GGAGCGTGAAGCTGAGCAGATGGGCGGTGAACTGGAGAGGCTGCGGGCAGCACTGATCAAGAGCCAGGGC CAGCAGCAAGAGGAGCGTGGGCAACAGGAGAGGGAGGTAGCACGGCTGACCCAGGAGCGGGGCCAGGCCC AGGCTGACCTGGCTCAGGAGAAGGCAGCCAAGGCAGAGCTGGAGATGCGACTGCAGAACACCCTCAATGA GCAGCGTGTGGAGTTTGCTGCCCTACAGGAAGCACTGGCCCATGCCCTGACAGAAAAGGAAGGGACAGAC CAAGAGCTTGCCAAACTTCGAGGGCAGGAGGCAGCTCAAAGAACAGAGCTGAAGGAGCTTCAACAGACTT TGGAACAGCTGAAAATACAGTTAGTGAAGAAAGAAAAAGAGCACCCTGCAGGGGGTGCCAGTGGAGAAGA TGCTTCTGGCCCAGGAACCCAGTCGGAGACAGCTGGAAAGACAGATGCACCAGGCCCTGAGTTGCAGGCA CTTCGGGCAGAGATCAGTAAGCTAGAGCAGCAGTGTCAGCAGCAGCAGCAACAGGTGGAAGGCCTGACAC ACAGCCTCAAGTCTGAGCGGGCTTGCCGGGCCGAGCAAGACAAAGCCCTGGAGACACTGCAGGGCCAGTT AGAAGAGAAGGCTCGAGAGCTAGGGCATAATCAGGCTGCCTCAGCTTCAGCTCAGAGAGAGTTGCAAGCC CTCCGTGCTAAAGCCCAGGACCATAGCAAAGCTGAGGAAGAGTGGAAGGCCCAGGTAGCCCGGGGCCAGC AGGAGGCTGAAAGAAAAAGCAGTCTTATCAGCAGCTTGGAAGAGGAGGTATCCATCCTGAATCGCCAAGT CCTAGAGAAGGAAGGGGAGAGCAAGGAGTTGAAGCGCCTGGTTGTAGCTGAATCCGAGAAGAGCCAGAAG CTGGAAGAGAGGCTGCGCCTGCTTCAAGTAGAAACAGCCAGCAATAGTGCCAGGGCAGCAGAACGCAGCT CAGCTCTACGAGAGGAGGTCCAGAGCCTCCGGGAAGAGGTGGAGAAACAGCGTGTAGTTTCAGAGAACTC GCGGCAGGAGTTGGCCTCACAAGCAGAGCGAGCTGAGGAGTTGGGCCAAGAATTGAAGGCCTGGCAGGAG AAGTTTTTCCAGAAGGAGCAAGCTCTCTCTGCCCTGCAGCTGGAGCACACCAGCACACAGGCCCTGGTAA GTGAGCTGCTGCCTGCCAAGCACCTTTGTCAGCAGCTGCAAGCTGAGCAAGCGGCTGCCGAGAAGCGCTT CCGGGAGGAGCTCGAGCAGAGCAAGCAGGCAGCTGGTGGGCTCCAGGCAGAGCTGATGCGGGCCCAGCGG GAGCTCGGAGAGCTCGGATCTTTGCGGCAGAAGATAGTTGAGCAGGAGCGAGCAGCCCAGCAGCTGCGGG CAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCCCATGGCCTGCTGGCAGAGGAGAA CCGGGGGCTGGGAGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTAGAACTAGATCAAGCCCGGGAG AAATATGTCCAGGAGTTGGCTGCTGTGCGTACTGATGCTGAGACCCACTTGGCTGAAATGCGACAAGAAG CACAGAGTACTAGCCGGGAGCTGGAGGTGATGACTGCCAAGTACGAAGGTGCCAAGGTGAAGGTCCTAGA GGAAAGGCAGAGGTTCCAAGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTAAGTAAGAAGCTG ACAGAGCATGACCAAGCCAGCAAGGTGCAGCAACAGAAGCTAAAGGCTTTCCAGGCCCAGAGAGGTGAAA GCCAGCAAGAGGTCCAGCGCCTCCAGACCCAGCTGAACGAGCTGCAGGCCCAGCTGAGCCAGAAGGAGCA GGCAGCTGAGCATTACAAGCTCCAGATGGAGAAAGCCAAGACCCATTATGATGCCAAGAAGCAGCAGAAC CAAAAGCTGCAGGAACAGCTTCAAGACTTGGAGGAACTGCAGAAGGAGAACAAGGAGCTGCGGTCTGAAG CTGAACGTCTGGGCCGTGAGCTGCAGCAGGCAGGGCTGAAGACCAAGGAAGCTGAACAGACCTGCCGTCA CCTTACAGCCCAGGTGCGCAGCCTGGAGGCCCAGGTTGCCCATGCAGACCAGCAGCTTCGTGACTTGGGC AAATTCCAGGTGGCAACTGATGCCTTAAAGAGCCGTGAGCCTCAAGTGAAACCCCAGCTGGACTTAAGTA TTGACAGCCTGGATCTGAGTTTGGAGGAGGGAACCCCATGCAGTGTGGCTAGCAAGCTGCCTCGTACCCA ACCAGATGGTACCAGTGTCCCTGGAGAGCCAGCTTCACCTATCTCCCAGCGCCTGCCCCCCAAAGTGGAA TCCCTGGAGAGCCTCTACTTCACCCCCACTCCTGCTCGGGGTCAGGCCCCCTTGGAGACTAGCCTTGACT CTCTGGGGGATGCCTTCCCTGACTCCGGCCGTAAGACCCGCTCTGCTCGCCGCCGTACCACACAAATCAT CAACATCACTATGACCAAGAAATTAGAATTGGAAGAGCCAGACAGCGCCAACTCATCCTTCTACAGCACG CAGTCTGCTCCTGCTTCCCAGGCCAACTTGCGAGCCACTTCCTCCACTCAGTCTCTGGCCCGCTTGGGTT CTCCTGATGATGGCAACTCCGCTCTGCTTAGCCTGCCTGGCTACCGGCCTACCACACGAAGCTCTGCTCG TCGCTCCCAGGCCAGGATGTCCAGTGGGGCCCCCCAAGGGAGGAATAGCTTCTACATGGGCACTTGCCAG GATGAGCCCGAGCAGCTGGATGATTGGAACCGCATCGCAGAGTTGCAGCAGCGCAACCGAGTATGCCCCC CGCACCTGAAGACCTGCTACCCTCTGGAGTCCAGGCCTACCCTGAGCCTGGCCACCATCACTGATGAGGA GATGAAGACTGGAGACCCCAGGGAAACCTTGCGTAGAGCCAGCATGCAGCCAGCTCAGATTGCTGAGGGT GTTGGCATAACTACCAGGCAGCAGCGCAAACGAGTTTCCTCTGAGACCCACCAGGGCCCTGGCACTCCTG AGTCCAAGAAAGCTACCAGCTGCTTTCCACGCCCCATGACCCCCAGGGACCGGCATGAAGGACGTAAGCA GAGCAGCACTGCTGACACCCAGAAGAAAGCAGCTCCAGTTCTCAAACAGGCGGACCGGCGTCAGTCAATG GCTTTCAGCATCCTTAACACACCTAAGAAGCTGGGAAATAGTCTTCTAAGGCGAGGAGCTTCAAAGAAGA CCCCAGCAAAGGTCTCCCCCAATCCTCGCAGTGGAACCCGCCGTTCTCCACGCATTGCCACCACCACAAC TGGCACTGCTACTGTTGCCACCACTCCTCGGGCCAAGGGCAAGGTGAAGCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133947
Insert Size	6285 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133947.3, NP_598708.3
RefSeq Size	7214 bp
RefSeq ORF	6285 bp
Locus ID	101706
UniProt ID	E9Q7G0
Gene Summary	Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:19255246, PubMed:24109598, PubMed:26765568). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:26765568). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:24109598, PubMed:26765568). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (By similarity). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:26765568). Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (By similarity). Required for epidermal differentiation and hair follicle morphogenesis (PubMed:26765568).[UniProtKB/Swiss-Prot Function]

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