Myof (NM_001099634) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310004N10Rik; 2310051D19Rik; E030042N20Rik; Fer1; Fer1l3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225147 representing NM_001099634 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGAGTGATTGTGGAATCTGCCACTAACATCCCTAAAACAAAATTTGGGAAGCCGGATCCTATCG TTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAACTCAACCCTGTCTGGAA TGAGATTTTGGAATTTGACTTGAGGGGGATACCACTGGACTCTTCATCCTCCCTGGTGATTGTTGTCAAA GATTTTGAGACAATTGGACAAAATAAATTAATTGGCACAGCAACTGTCTCCCTGAAGGACCTGATTGGGG ACCAGAACAGGTCTTTGCCCTACAAACAGACCTCCCTGCTAAATGAAAAGGGACAAGACACTGGGGCCAC CATTGACCTGGTGATCGGCTATACCCCACCTTCAGCTCCACATCCAAATGATCCGAGCGGGACCAGTGTG CCAGGCATGGGAGAAGAAGAGGAAGAAGATCAAGGAGATGAAGACAGGGTAGATGGCATAGTCAGAGGCC CTGGGCCCAAGGGGCCATCTGGGACAGTGTCAGAAGCTCAGCTGGCTCGGAGGATCACCAAAGGGAAGAG CAGCCGAAGGATGCTGTCCAACAAGCCACAGGATTTCCAGATCCGTGTCAGAGTGATCGAGGGCCGTCAG TTATGCGGCAACAACATAAGGCCCGTGGTGAAAGTTCACATCTGTGGTCAGACGCACCGCACGAGAATCA AGAGAGGGAACAATCCGTTTTTTGACGAGTTATTTTTCTACAATGTGCACATCACACCCTCTGAGCTCAT GGATGAGATCATCAGTATCCGGGTTTATAATTCCCATTCTTTGAGGGCAGATTGTCTGATGGGGGAATTT AAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCACGCTGTCATGAGGAAATGGCTCCTTCTCAATG ACCCAGAAGACACCAGCTCAGGCGCAAAAGGTTACATGAAAGTCAGCATGTTTGTCCTGGGAACCGGAGA CGAACCCCCTCCTGAGAAACGAGACCGTGACAATGACAGTGATGACGTGGAGAGCAACTTGTTACTTCCG GCAGGCATTGCCCTCCGGTGGGTGACCTTCATGCTGAAGATCTACCGAGCCGAGGACATCCCCCAGATGG ATGATGCCTTCTCACAAACAGTGAAGGAAATATTTGGTGGCAACGCAGATAAAAAAAATCTTGTGGATCC TTTTGTAGAAGTCTCCTTTGCTGGGAAAAAGGTGTGCACAAATATAATTGAGCGGAATGCAAACCCTGAA TGGAATCAAGTTGTCAACCTTCAGATCAAGTTTCCCTCCATGTGTGAAAAAATAAAACTAACAGTATATG ACTGGGACCGGCTTACTAAAAATGATGTAGTTGGGACCACGTACCTGTACCTCTCTAAAATTGCTGCCTC TGGGGGAGAAGTGGAAGCAACTACAGGAGAGACAGAGGTGGGGTTTGTCCCAACATTCGGACCTTGTTAT TTGAACCTTTACGGAAGCCCCAGAGAGTACACAGGATTTCCAGATCCCTATGATGAGCTCAACTCAGGAA AGGGAGAAGGGGTGGCCTACAGAGGCAGGATCTTCGTGGAACTGAACACGTTCCTTGAAAAGAAACCACC AGAAAAGAAGCTGGAGCCCATTTCCAGTGATGACCTTCTGGTTGTTGAGAAGTACCAGCGAAGGCGGAAG TATAGCCTGTCCGCCGTGTTCCATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAG TCAGCATTGGGAACTATGGCAACAAGTTTGATGCAACCTGTAAACCTTTGGCATCAACCACTCAATACAG CCGTGCAGTATTTGACGGGAACTACTATTACTACTTACCTTGGGCCCACACGAAGCCCGTGGTCACCCTG ACTTCCTACTGGGAGGATATTAGTCACCGCCTGGATGCAGTGAACACTCTCCTAGTCATGGCAGAACGGC TGCAATCAAACATAGAGGCTGTAAAATCAGGGATCCAGGGTAAAATTCCTGCAAACCAGTTGGCGGAGGT GTGGCTGAAGCTGATAGATGAAGTTATAGAAGACACGAGATACACCTTGCCTGTCACAGAAGGAAAAGCC AACGTCACGGTTCTGGATACTCAGATCCGAAAGTTGCGGTCCAGGTTTCTCTCCCAGATACACGAGGCAG CTCTGAGGATGAGATCTGAAGCCACGGATGTGAAATCCACCTTGTTGGAAATCGAGGAGTGGCTTGATAA ACTGATGCAGCTTACTGAAGAGCCTCAGAACAGCATGCCTGACATCATCATCTGGATGATCCGTGGAGAG AAAAGACTGGCTTATGCACGAATCCCTGCCCACCAAGTCTTGTACTCCACCAGCGGTGGGAACGCCTCTG GAAAATACTGTGGGAAAACCCAAACCATCCTTCTGAAGTATCCACAAGAAAAAACCAATGGGCCCAAAGT GCCTGTGGAGCTGCGCGTGAACATCTGGCTGGGTTTAAGTGCTGTGGAAAAGAAGTTCAATAGCTTCGCG GAAGGCACCTTCACCGTCTTTGCTGAAATGTATGAAAACCAAGCCCTTGTGTTTGGGAAGTGGGGCACCT CTGGACTGGTGGGACGCCATAAATTCTCTGATGTCACAGGAAAAATAAAACTCAAGAGAGAATTCTTTTT ACCTCCCAAAGGCTGGGAGTGGGAAGGAGACTGGGTGGTTGACCCTGAACGAAGCCTCCTGACTGAGGCT GACGCAGGTCACACCGAGTTCACTGATGAAGTCTACCAGAATGAGAATCGCTACCCCGGAGGCGAGTGGA AGCAGGCAGAGGACACCTATACGGATGCGAATGGAGATAAGGCGGCATCACCCAGTGAGATGACCTGCCC TCCAGGTTGGGAGTGGGAGGATGACGCATGGATATATGACATAAATCGAGCAGTGGATGAGAAAGGCTGG GAGTATGGGATTACCATTCCTCCTGATAATAAGCCCAAATCCTGGGTTGCAGCAGAAAAGATGTATCACA CTCACAGGCGGCGGAGACTGGTCCGGAAACGCAAGAAAGATCTGACACAGACGGCTTCTAGTACCGCAAG GGCCATGGAAGAACTTGAAGACCGGGAGGGCTGGGAATATGCTTCGTTAATCGGCTGGAAATTCCACTGG AAACAGCGCAGTTCAGACACCTTTCGCCGCAGGCGCTGGAGGAGGAAAATGGCCCCTTCTGAGACACACG GTGCCGCTGCCATCTTTAAACTCGAAGGTGCCCTTGGGGCAGACACCACAGAGGATGGAGAAGAGAAGGG CCCGGAGAAGCAGAAGCACAGCGCCACCACTGTGTTTGGAGCAAACACGCCCATTGTTTCCTGTAATTTC GACAGAGTCTACATCTACCACCTGCGCTGCTACATTTATCAAGCCAGGAACCTCATGGCTTTGGACAAAG ACAGCTTTTCAGATCCATACGCCCATGTCTCCTTCCTCCATCGAAGCAAAACCACTGAGATCATCCACTC CACCCTGAATCCCACCTGGGACCAAACCATTATATTCGATGAGGTTGAAATCTTTGGGGAACCCCAAACA GTTCTACAGAACCCACCCAACGTTACCATAGAACTTTTTGACAATGACCAAGTGGGCAAAGATGAATTTT TAGGTCGAAGCATTTGCTCTCCCTTGGTGAAACTGAACTCAGAAACGGACATCACACCTAAGCTTCTCTG GCATCCTGTTATGAATGGAGACAAGGCCTGTGGGGATGTCCTCGTAACAGCAGAGTTGATTCTGAGGAAC AAGGATGGCTCCAACCTTCCCATCCTCCCCTCTCAAAGAGCACCAAACTTATACATGGTCCCTCAGGGGA TCAGGCCTGTGGTTCAGCTCACTGCCATTGAGATCCTAGCTTGGGGCTTAAGAAATATGAAAAACTACCA GATGGCTTCTGTCACATCACCCAGCCTGGTGGTGGAATGCGGAGGAGAGAGAGTAGAATCGGTGGTGATC AAAAGCCTTAAGAAGACACCCAACTTCCCAAGTTCTGTTCTCTTCATGAAAGTGTTCTTGCCCAAGGAAG AATTGTACATGCCTCCGCTGGTGATCAAGGTCATTGATCACAGGCAGTTTGGGCGGAAGCCTGTGGTTGG CCAATGCACCATTGACCACCTGGACCGCTTTCGGTGTGATCCATATGCAGGAAAGGAGGATATTGTACCA CAACTAAAAGCCTCTCTGATGTCTGCACCTCCATGCCGGGAAGTCGTTATTGAAATAGAAGACACCAAAC CATTACTGGCTTCTAAGCTGTCGGAAAAGGAGGAAGAAATCGTTGACTGGTGGAGTAAATTTTATGCTTC CTCTGGAGAGCATGAAAAATGTGGACAGTATATTCAGAAAGGCTATTCCAAACTCAAGATCTATGACTGT GAACTGGAGGATGTAGCCGACTTTGAAGGCCTGACAGATTTCTCAGATACCTTCAAGCTGTACAGAGGCA AGTCCGATGAGAATGAAGATCCATCTGTGGTAGGAGAGTTTAAAGGCTCCTTCCGAATCTACCCCTTGCC GGATGACCCCAGTGTGCCAGCCCCTCCCAGGCAGTTTCGGGAACTGCCTGACAGTGTCCCACAGGAATGC ACTGTTAGGATTTACATTGTTCAAGGCTTACAGCTCCAGCCTCAGGACAACAATGGCCTGTGTGATCCTT ACATAAAAATAACCTTGGGCAAGAAAGTAATTGAAGACCGAGACCACTACATTCCCAACACTCTCAACCC AGTGTTTGGCAGAATGTACGAACTGAGCTGCTACTTGCCTCAAGAGAAAGACCTGAAGATCTCTGTCTAT GACTACGATACCTTCACCCGAGATGAGAAGGTGGGAGAGACAACCATTGACCTGGAGAACCGCTTCCTTT CCCGGTTCGGGTCTCACTGCGGAATCCCTGAGCAGTACTGTGTTTCCGGAGTAAATACCTGGCGAGATCA GCTGAGACCAACGCAGTTGCTTCAAAACGTTGCAAGATTCAAAGGCTTCCCGCCACCAGTTCTTTCTGAA GACGGAAGCAGAATCCGATACGGAGGTCGAGACTACCATTTGGATGAGTTTGAGGCCAACAAAATCCTGC ACCAGCACCTTGGAGCCCCCGAAGAGCGTCTTGCCCTCCATATTCTCAGGACTCAGGGGCTGGTCCCTGA GCACGTGGAGACAAGGACTTTGCACAGCACCTTCCAGCCCAATATTTCCCAGGGGAAACTTCAGATGTGG GTGGATGTCTTCCCCAAGAGTTTAGGACCACCAGGCCCCCCTTTCAACATCACACCCAGGAAAGCCAAGA AGTACTACCTGCGTGTGATCATCTGGAATACCAAGGACGTTATCTTGGATGAGAAGAGCATCACTGGGGA GGACATGAGTGACATCTACGTCAAAGGCTGGATTTCCGGCAGCGAAGAAAACAAACAGAAAACAGATGTC CACTACAGGTCCTTGGATGGTGAAGGCAATTTTAACTGGAGATTTGTTTTCCCATTTGACTACCTCCCTG CTGAGCAGCTCTGTATAGTCGCTAAAAAAGAGCATTTCTGGAGTATTGATCAAACAGAATTTCGAGTCCC TCCTCGGCTGATCATTCAGATATGGGACAATGACAAGTTTTCCCTGGACGACTACTTGGGTTTTCTGGAA CTTGACTTACATCGTACGATCATCCCAGCCAAGACATCAGAGAAATGCAGTTTGGACATGATTCCAGACC TCAAAGCCATGGACCCTCTTAAAGCTAAGACTGCCTCACTCTTTGAACAGAGGTCCATGAAAGGATGGTG GCCGTGCTACGCAGACAAAGATGGCACCCGGGTGATGGCTGGGAAAGTGGAGATGACACTGGAGGTGCTC AACGAGAGGGAGGCCGACGAGAGGCCAGCCGGGAAGGGGCGGAGTGAACCCAACATGAACCCCAAGCTTG ACCCACCAAACCGACCGGAAACCTCCTTCCTCTGGTTCACGAACCCTTGCAAGACCATGCGCTTCATCGT GTGGCGCCGGTTCAAGTGGGTGATCATCGGCCTGCTTCTGCTGCTCATCCTGTTGCTGTTCGTGGCTGTG CTCCTGTACTCTCTTCCGAACTATTTATCAATGAAGATTGTGAGGCCGAATGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001099634
Insert Size	6147 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001099634.1, NP_001093104.1
RefSeq Size	7094 bp
RefSeq ORF	6147 bp
Locus ID	226101
UniProt ID	Q69ZN7
Gene Summary	The protein encoded by this gene is a member of the ferlin family of proteins, which have been implicated in fusion events in muscle tissue. Members of this family have a carboxy-terminal single pass transmembrane domain and multiple C2 domains, which bind negatively charged phospholipids in the presence of calcium ions. This gene is expressed at high levels in myoblasts and upregulated in damaged skeletal muscle. Mice deficient in this protein display defects in myoblast fusion, muscle regeneration, and angiogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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