Phf3 (NM_001081080) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310061N19Rik; mKIAA0244
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225133 representing NM_001081080 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATAGTTGATACATTTAATCATTTAATTCCTACTGAACACTTAGATGATGCCCTATTTCTAGGAT CCAACCTGGAGAATGAAGTCTGTGAGGATTTTAGTACAAGTCAAAATGTCTTAGAGGACTCGCTGAAGAA CATGCTCAGCGATAAGGATCCTATGCTAGGATCTGCAAGTAACCAGTTCTGTTTGCCTGTTTTGGATAGC AATGATCCCAATTTCCAGATGCCTTGTTCAACAGTTGTTGGTCTTGACGATATTATGGATGAAGGAGTTG TTAAAGAAAGTGGTAATGATACCATTGATGAAGAAGAATTGATTTTACCTAACAGGAGTTTGAGGGACAG AGTAGAAGACAATTCAGTAAGATCACCAAGAAAATCACCTCGTTTAATGGCACAAGAACAAGTAAGAAGT TTGCGACAAAGCACTATTGCCAAGCGTTCAAATGCAGCAACTCTAAGCACAAAAAAGCCATCTGGGAAGA CCTTATCTACCTCTAAAGTAGGGGTGAAGCCAGCAGAAAGATGTCAGGGTAAAGAAGAAGTTTATGCCTC ACTGAAATCTGAACACCCTAAGGAGAGTAGAAGGAGTGGCCGGCATGCTGAACAGATGGACGTGGCGCCA GAAGTCTCAGCATCTTCAGTTGATTCTTCAGTGTCGTCTTGTGCTGGAATGAAGGAGGAGGCTGAGTTTG ATCCCAAACATGCATGCAATAATCAGGGTGAAGTGAACGTGCCATCTCCTGAGTTAGATTGTCCACTTCT CTCAGAGACTAGTGCTAGTGTGGAAGAGAAGAACATTGAGGCTCTGATGGAATGTAAAGCTAAGACTAAC AGTAGCCCGTTATTTAAGTTTCCAGTTAGAGAAGATGAGCAGAGTGATCTTGTTTCGGGTGAACTGAATG ACACCATTGAAGGAAAGGATGCAGGGGGAAAACCTGATCAAGAATCAGAGGAGGTGAAGTTTCCCTGCGA AGGTGACCAGACGGCCGAGGAACCCGAATCTTCTGATGTGTCTAGTGACTCTGCTTGTGCCAATAAAAAT AAGGCAGAAAAGAATGAAGGGGCAGAATGTCATTTGGAATTGAAGAATACTGTGGATATTGTTGATAAAC CTGAGAACTCACCTCAGAGAAATGAGTTGGAAACACTGGGCTATGGTGAGGACACGGAGTCTAATGATGC TCGGTTACAGAGCACAGAGTTCAATAAGTCAGATTTGGAGGAAGTTGATGCTTGTGCTTTTGAACCGGAA GCTAGCACTTTAGAAAATACTATTTGTGATGTTCTTGACCAAAATTCAAAGCAATTGAATATTACTCAAA GTATTAAAATGGAAACAGCCAATCTTCAGGATGATAGAAGTGGTTTAGAGCCCAAAAACATAAAACCCAA ACACATAAAATCTGTAACTCATTCTAAACAAAGCATGACCACAGAGACACCCAGAAAAACTGTGGCAGCA AAGCATGAAGTAGGTCATAGCAAAACTAAATCTAATGTCAAGGCTGTGAAGCGAAATTCTGGTGAGCCGG AACCCCAGCCTGATTCTCAGAGGCCAGTCAAAGTGAGAAAAAAACAAGGTGATAAGGTTTGGAAGAGTCA GAGTTGTAATTCTGGAGTTAAATCTGTGAAAAGCCAAGCTCATTCTGTATTGAAAAGAATGCCCCAGGAT CAAAACACAATGCAGATTTCCAAACCTTTAACTCATCCTCATAGTGATAAGCTTCATGGTCACTCTGGCT TCTCTAAGGAGCCTCCCCATCCTGTACAAACTGGACACTTGGTGCATTCCAGTCAAAAACAGAGCCAGAA GCCTCAGCAGCAGGCAGCTGTGGGGAAGGGCAGTAGCCATGTGAAGGATGAGCATGACCATCCAGTCAGT GAGCATCTTAAGGAGGACGATAAACTGAAGCCAAGAAAACCTGATAGGAACCTGCAGCCTCGCCAAAGGA GAAGCAGCCGAAGTTTTTCTCTGGATGAGCCTCCATTGTTCATCCCAGACAACATAGCTACTGTAAAAAA AGAAGGGTCAGATCAGACCACCTCAATTGAAAGCAAATATATGTGGACTCCAAGCAAGCAATGTGGGTTT TGCAAAAAACCACATGGCAACAGGTTTATGGTCGGCTGTGGGAGATGTGATGACTGGTTTCATGGAGACT GTGTTGGATTAAGTCTTTCTCAAGCACAGCAAATGGGAGAGGAAGACAAAGAATATGTTTGTGTAAGGTG CTGTGCTGAAGAAGATAAAAAGACTGACATACTAGACACAGAGATTTTTGAAGCCCAAGCTCCCATCGAA GCCCACAGCGAAGATAAAAGGATGGAGTGTGGAAAGCTGACATCATCGAAGCACGCAGTCACTGACGATA AACACAGACACTCAGATGACCCTGGGAAGCACAAGGTCAAAATTTTAAAACGGGAGTCTGGTGAGGGAAA AACTTCCTCTGACAGTAGAGATAATGAAATTAAAAAATGGCAGCTAGCCCCTCTTCGCAAGTTGAGCCAA CCACATTTACCTCGGAGATCATCAGAAGAAAAAAGTGAAAAAATAGCAAAGGAATCTACAGCTCTTGCCT CTACAGGAGAGAGAGTGGCGAGATCAGGTACTCATGAGAAGCAAGAGACAAAAAAGAAGAAAATGGAAAA GGGTGGACCTAATGTGCACCCGCCTGCTGCTACTTCCAAGCCTTCTGCAGATCAGATCAGACAGAGTGTC CGACATTCTCTCAAAGATATTCTTATGAAAAGACTTACAGATTCAAACCTGAAGATCCCAGAGGAAAAGG CAGCAAAGGTTGCCACAAAAATTGAAAAAGAACTTTTCTCTTTTTTTCGGGACACGGATGCCAAGTATAA GAATAAATATAGAAGCTTGATGTTTAATCTAAAAGATCCTAAAAACAATATATTATTTAAGAAAGTTCTA AAAGGAGAAGTAACCCCTGATCATCTTATAAGAATGAGCCCAGAAGAACTAGCCTCCAAAGAGCTGGCTG CGTGGAGACGAAGGGAGAACAGACACACCATAGAGATGATTGAGAAGGAACAGAGAGAAGTGGAAAGACG ACCAATCACGAAAATAACTCACAAAGGTGAGATAGAAATCGAGAGTGACGCCCCGATGAAGGAGCAGGAA GCAGCCATCGAGATCCAGGAACCGTCAGCTAATAAGTCAATGGAGAAGCCAGATGTGTCTGAGAAACAAA AAGAAGAGGTTGACTCCACATCTAAAGACACCACTAGTCAACACAGACAGCATCTCTTTGACCTGAACTG CAAAATATGCATAGGTCGAATGGCACCACCTATAGATGATCTTTCTCCAAAAACTGTGAAAGTTGTTGTA GGAGGAGCCCGGAAACACTCAGACAACGAAGCAGAGAGTCTAGCAGATGCGTTGTCTTCAACTACGAATA TTCTGACCTCTGACCTCTTTGAGGAGGAAAAGCAAGAATCTCCCAAGTCAACGTTTTCCCCCACTCCACG GCCAGAGATGCCTGGAACTGTGGAAGTTGAGTCAACCTTTCTGGCTCGATTGAACTTCATCTGGAAAGGT TTTATCAATATGCCCTCTGTGGCAAAGTTTGTTACCAAAGCCTACCCCGTGTCTGGATCCCCTGAGTACT TGACAGAGGACCTACCAGACAGCATTCAAGTAGGTGGCAGGATATCGCCTCAGACAGTTTGGGATTATGT GGAAAAAATAAAAGCATCAGGCACCAAGGAAATCTGTGTGGTTCGCTTCACACCAGTAACTGAAGAAGAT CAGATTTCTTACACTTTGCTGTTTGCGTACTTCAGTAGCAGAAAGCGCTATGGTGTGGCTGCTAACAACA TGAAGCAGGTTAAAGACATGTACCTCATTCCATTGGGCGCTGCAGATAAAATCCCACACCCTCTTGTGCC TTTTGATGGACCTGGACTTGAGCTGCACAGACCTAATCTGTTGTTGGGCCTGATTATCCGTCAGAAGCTG AAGCGGCCGCACAGCGCTAGTGCAGGCCCCAGTCACACAGGCGAGACTCCTGAGAGCGCCCCAATAGTGT TGCCCCCCGACAAGAAAGGTAAAATGGAGTCATGCACAGAGGAAGCAGCAGAGGAAGAGAGTGACTTTTT CAATTCCTTTACAACTGTGTTACACAAGCAGAGAAATAAGCCCTCACAGCCTCTTCAGGAAGACCTGCCA ACAGCAGCTGAACCTCTGATGGAAGTCACCAAGCAGGAGCCACCAAAACCCTTAAGGTTCCTTCCTGGGG TCCTGATTGGCTGGGACAATCAACCTTCTACTCTGGAATTAGCAAATAAGCCTCTTCCTGTGGATGACAT ACTCCAAAGCCTTTTGGGCACCACTGGTCAAGTATATGAGCAGGCTCAGCCACTTGTAGAACAAAGCACT CTTAAAGAAATTCCTTTTATAAATGATCAAGCTAACCCAAAAGTAGAGAAAATAGATAAAGTGGAGGTGA CTGAAGGTGACGCCAAGGAGATAAAAGTTAAAGCAGAAAATATTTCAGTGTCTACAAGTAAAAACTCAGG AGAAGAGACTTCATCGGTAGGTTCATCTTCCATTTCTCCAGGGCCTTTGGCAAGTCTCAGTCTGAGAGGG AAACCACCAGATGTTTCTACAGAAGCATTCTTAACAAATTTATCAATTCCCTCAAAACAAGAGGAAAGTG TGGAAAACAAAGAGAGAACATTAAAAAGACTGCTGCTCCAAGATCAAGAGAATAGTTTGCAAGACAATAG GACTTCAAGTGACTCTCCCTGCTGGCCTGGCACTGGGAAGGGAGGCATGGATGGGGATGGGAGCGGGAGC GGGAGCGGGAGCGGGAGCGGCAGTGAGGGTCCAGTGGCTAACACAAGAGCCCCACAGTTTATCAACCTGA AAAGGGACCCTAGGCAAGCAGCAGGACGAAGTCAGCAGACAGCTTCTGAAAGCAAGGATGCAGAGAGCTG CAGAAATGGAGACAAGCATGCTGCGTCTGCTCCACCACACAACAAAGAGCCCTTAGCAGAGGCTGTCGGT GGAGAGGGAAAGCTGCCTTCGCAGGAGAAGAGCTCATGTGTAGAGCAGAACGACGATTCGGAAGCTGCAC CAAACTCATCCTCAGTAGAAAACTTAAATTCGTCACAAGCAGAACAAGCCAATCCTTCACAAGAGGATGT TTTAACACAAAATATTGAAACGGTCCACCCGTTTAGAAGAGGATCGGCTCCAACATCATCTCGTTTTGAA GGTGGAAATACCTGCCAATCAGAATTTCCGTCTAAAAGCGTTAGCTTTACTTGCAGGAGTAGCAGCCCTC GCGCGAGTACAAACTTTTCACCTATGAGGCCACAGCAGCCCAATCTCCAGCACCTCAAGTCCAGTCCTCC TGGATTCCCATTTCCAGGACCCCAAAACTTCCCTCCGCAAAATATGTTTGGATTCCCACCACATTTGTCA CCTCCGTTACTTCCCCCTCCAGGCTTCGGCTTCCCTCAAAATCCCCCCATGGTTCCTTGGCCACCTGTTC ATGTCCCAGGCCAACCACAACGCATGATGGGGCCCCTTTCACAAGCATCACGGTACATGGGCCCACAAAA TTTTTATCAGGTAAAAGATATTCGGAGACCAGAAAGGCGCCATAGTGACCCATGGGGTAGGCAAGACCAA CAACAACCAGATAGGCCATTTAATAGGGGTAAAGGGGACCGACAGAGATTTTACAGTGATTCGCACCACT TGAAAAGAGAGCGTCACGACAAGGATTGGGAGCAGGAATCCGAAAGGCACAGACACAGAGACAGAAGCCA AGAAAGGGACAGAGACAGAAAAAGCAAAGAGGAAGCAGCGGCACACAAAGACAAGGAGAGACCGCGGCTC TCACACGGCGACCGGGCACCAGATGGGAAAGCAAGCAGAGATGGTAAGAGTGCAGACAAGAAACCGGACA GGCCTAAAGGCGAAGACCACGAAAAGGAGAAAGAGAGAGACAAGAGCAAGCACAAAGAAGGGGAGAAGGA CAGAGAGCGGTACCACAAAGACAGGGACCACACCGACAGAGTGAAAAGCAAAAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081080
Insert Size	6078 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081080.1, NP_001074549.1
RefSeq Size	7561 bp
RefSeq ORF	6078 bp
Locus ID	213109

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