Trip12 (NM_133975) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110036I07Rik; 6720416K24Rik; AA410158; Gtl6; TRIP-12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225130 representing NM_133975 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTCATAGTTCCACAACCAGA GGATCCAGACAGAGCCAATACTTCAGAAAGGCAGAAAACGGGGCAGGTGCCTAAAAAAGACAATTCTCGA GGAGTGAAACGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCGAAAAAGCCCAGAG CATTTCAGCATATTGAGTCTTTCTCAGAAACCAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGA CCAGGAGCAACAACTGAAATCTGCACAATTGCCGTCAACAAGCAAGGCTCACACCAGAAAGAGTGTGGCT GCTGGTAGTTCACGGAATCAGAAAAGAAAGAGGACAGAGAGTTCTTGTGTTAAAAGTGGTTCTGGGTCCG AATCAACTGGTGCCGAAGAGAGATCTGCAAAACCCATCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAA AGCTGGGTGTAGCACCATCACCGATTCTTCTTCTGCTGCCTCTACTTCCTCATCATCTTCAGCTATAGCC TCGGCCTCTTCTACTGTACCAGCAGGTGCCAGGGTGAAACAAGGAAAAGACCAGAACAAGGCCAGGCGTT CCCGTTCAGCATCCAGCCCTAGTCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTC TTCAAAATTTGATTGGGCTGCTCGCTTCAGCCCCAAAGTTAGCCTACCTAAAACAAAACTGTCTCTTCCA GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA AGTCCACCAAGAAACGCAGTGAGTCCCCACCTGCTGAGCTCCCCAGTTTGAGGAGGAGCACACGCCAAAA GACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAGAGAGGAGCAGCTGAGGCC CGGCGGCAAGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGACAGTAAATTCCTCAGCTGCCCGGACAG ATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGA GAGTGAATCGGATGATTCTGAGATGGGACGATTACAAGCTCTGTTAGAGGCCAGGGGTCTTCCTCCTCAC CTATTTGGTCCTCTCGGGCCTCGGATGTCACAACTTTTCCATAGAACAATTGGAAGCGGAGCTAGTTCTA AGGCCCAGCAGCTTCTTCAAGGACTACAAGCCAGTGATGAAAGTCAACAGCTTCAGGCCGTCATTGAAAT GTGTCAGTTATTGGTCATGGGGAATGAGGAGACACTAGGAGGGTTTCCTGTGAAGAGTGTTGTTCCAGCT TTGATAACATTACTGCAGATGGAACATAATTTTGACATTATGAATCATGCTTGCCGAGCCTTAACATATA TGATGGAAGCTCTCCCCCGGTCATCTGCTGTTGTAGTGGATGCTATCCCTGTTTTCTTAGAAAAGTTGCA AGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGCGACACAGT AAAGCCATTTTACAAGCGGGTGGTTTGGCAGATTGCTTGTTGTATCTAGAATTCTTCAGCATCAATGCCC AAAGAAATGCACTAGCAATTGCAGCCAATTGCTGCCAGAGTATCACACCAGATGAGTTTCATTTTGTGGC AGATTCCCTACCATTGCTTACCCAAAGGCTAACTCATCAGGACAAAAAGTCAGTAGAAAGTACTTGCCTT TGTTTTGCACGTTTAGTGGACAACTTTCAACATGAGGAGAACTTACTGCAACAGGTTGCTTCCAAAGATC TACTTACAAATGTTCAACAATTGTTGGTAGTGACTCCACCGATTTTAAGTTCTGGGATGTTTATTATGGT GGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACATTAGCTGTTCAACTTATGAAACAAAACATT GCAGAAACACTTCACTTCCTGCTATGTGGTGCCTCCAATGGAAGTTGTCAAGAGCAGATTGACCTTGTTC CACGAAGTCCTCAAGAGCTGTATGAGCTGACATCTTTGATTTGTGAACTTATGCCATGTTTACCAAAAGA AGGCATCTTCGCAGTTGATACCATGTTGAAAAAGGGAAATGCACAGAACACAGATGGTGCAATATGGCAG TGGCGGGATGACCGAGGCCTCTGGCATCCCTATAACAGGATTGACAGCCGGATCATTGAGGCAGCCCATC AGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGCA GCAAATCAATGAAGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAATCCCTTAGCCAATTCTAAC ACTAGTGGATATTCAGAGTTAAAGAAGGATGATGCTCGAGCACAGCTAATGAAAGAAGATCCGGAACTGG CTAAGTCTTTCATTAAGACCTTGTTTGGTGTTCTCTATGAAGTGTACAGCTCCTCAGCAGGCCCTGCGGT CAGACACAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCTGATGCTGAACTTCTGAAGGATGTT CTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCAATGCTATCCAGCCAAGACCTGAAGATAGTAGTTG GAGCTCTTCAGATGGCAGAAATCCTAATGCAAAAGTTACCTGATATTTTTAGTGTTTACTTCAGGAGAGA AGGTGTGATGCATCAAGTCAAACATTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCCCCCAAGGCATGT ACAAATGGGTCAGGATCCCTGGGATCTACAACACCAGCCAGCAGTGGGACTGCAACGGCAGCAACCAACG CCTCTGCTGATCTGGGGTCACCTAGCTTGCAGCATAGCAGAGATGATTCTTTGGATCTGAGCCCTCAAGG TCGATTAAGTGATGTTCTCAAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAATATTCCCCT CCAAGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCCT TCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGCGCACAGTCCAACAGCAACAATATCGAACC AGCACGAACTGCAGGAGTTAGTGGTCTTGCCAGGGCAGCCTCAAAAGACACCATATCCAATAATAGAGAA AAAATTAAAGGCTGGATTAAGGAGCAGGCACATAAATTTGTTGAGCGTTATTTTAGTTCTGAGAATATGG ATGGAAGCAACCCTGCCTTGAATGTCCTTCAGAGACTTTGTGCTGCAACTGAACAACTCAACCTCCAGGT GGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCTTCATTTGAA ATCCAACATAGTGGGTTTGTGAAACAACTGTTGCTGTATTTGACATCTAAGAATGAAAAGGATGCTGTCG GCAGAGAGATCCGGTTAAAACGTTTCCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCTGT TGGAAGAGTAGAACCAGTGGGCCATGCACCTTTGCTGGCATTGGTTCACAAGATGAACAACTGTCTCAGC CAAATGGAGCAATTTCCAGTCAAAGTACATGATTTCCCTAGTGGGAACGGAGCTGGAGGAAGCTTTTCTC TCAACAGAGGATCACAAGCTTTAAAATTTTTCAACACACATCAGTTAAAATGTCAGTTACAAAGACACCC AGACTGTGCAAATGTGAAGCAGTGGAAAGGCGGGCCTGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCT ATTGAGAGATACCTTGTGGTTAGAGGATATGGAAGAGTAAGAGAAGATGATGAAGATAGTGATGATGATG GGTCAGATGAAGAAATAGATGAGTCTTTGGCTGCTCAATTCTTAAATTCGGGAAATGTAAGGCACAGGCT ACAGTTTTATATTGGAGAACATTTACTACCATATAACATGACCGTGTATCAGGCAGTGCGGCAATTCAGT GTACAGGCTGAAGATGAAAGAGAATCTACTGATGATGAGAGCAATCCTCTGGGAAGAGCTGGAATTTGGA CAAAGACTCATACAATATGGTACAAACCTGTGAGGGAGGATGAAGAAAGTACTAAAGATTGTGTTGGTGG CAAAAGGGGGAGAGCCCAAACAGCTCCAACAAAAACTTCCCCCAGAAATGCAAAGAAGCATGATGAGTTA TGGCATGATGGAGTGTGCCCGTCAGTAGCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAA ATATCACCTTTGAAGACCCATCCTTAGATGTAATACTTCTTTTAAGAGTTTTACATGCCATCAGTCGATA TTGGTATTACTTGTATGATAATGCGATGTGCAAGGAGATTATTCCAACTAGTGAATTTATTAACAGTAAA TTAACAGCAAAAGCAAATAGGCAGCTTCAAGACCCTCTAGTAATCATGACAGGAAACATCCCAACATGGC TCACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGACAAATGCTTTTTTATGTAAC TGCATTTGATCGGGACCGGGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCT CAAGACAGCAGAGTTGCGCCTAGATTGGATAGAAAAAAACGTACTGTGAACAGAGAGGAGCTACTGAAAC AAGCTGAGTCTGTGATGCAGGACCTTGGCAGCTCTCGGGCCATGTTAGAAATCCAATATGAAAATGAGGT TGGTACAGGTCTGGGGCCAACACTGGAGTTTTATGCACTTGTTTCTCAGGAACTACAGAGAGCTGACTTG TGTCTCTGGAGGGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGAAGCCAAGAAGGGACCAAGTATATTC AAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCTAAGCCAGCTCACATCGCAAAGGTTAA GATGAAGTTTCGCTTCTTAGGAAAATTAATGGCTAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCT CTTGGCCTGCCCTTTTATAAGTGGATGCTGCGGCAGGAAACTTCATTGACATCTCATGATTTATTTGACA TTGATCCTGTTGTAGCCAGATCAGTATATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTGGAGCA GGATAAATCCCAGACCAAAGAGAGTCTACAATATGCATTGGAAACTCTTACGATGAATGGCTGTTCAGTG GAAGATCTGGGATTGGATTTTACGCTGCCTGGGTTTCCCAACATTGAATTGAAGAAAGGAGGGAAGGATA TTCCAGTTACTATCCACAATTTGGAAGAGTACCTGAGATTGGTGATATTCTGGGCACTAAATGAAGGTGT TTGTCGACAGTTTGACTCATTCAGAGATGGATTTGAATCGGTCTTCCCGCTCTGCCATCTTCAGTACTTC TACCCAGAAGAGCTGGACCAGCTCCTGTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACATTGATGG AATGCTGCAGGCCTGATCATGGCTATACTCATGACAGTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAG TAGTTTTGATAATGAGCAGCAGAGGTTGTTTCTCCAGTTTGTGACTGGTAGCCCACGATTGCCAGTTGGA GGATTTCGGAGTTTAAATCCACCTCTGACAATTGTGCGGAAGACATTTGAATCAACAGAAAACCCAGATG ACTTCTTACCATCAGTAATGACTTGTGTGAACTATCTTAAATTGCCGGACTACTCGAGCATTGACATAAT GCGGGACAAACTCTTGATAGCAGCAAGAGAAGGCCAACAGTCGTTCCATCTTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133975
Insert Size	6078 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133975.4, NP_598736.4
RefSeq Size	9632 bp
RefSeq ORF	6078 bp
Locus ID	14897
UniProt ID	G5E870
Gene Summary	E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.[UniProtKB/Swiss-Prot Function]

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