Fancm (NM_178912) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI427100; C730036B14Rik; D12Ertd364e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225128 representing NM_178912 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGACGGCAGAGAACGCTCTTCCAGACGTGGGGCCCGAGCCTCGTGCGGGGCTCCGGAGACTCGG GATGCGGCCAGCCGCGCTCGCCCGCGATGGCGGAGGCGCTGCCGGAGGAGGACGACGAGGTGCTGCTGGT GGCGGCGTACGAGGCTGAGCGGCAGCTGGATCCCGGGGATGGCGGCTTCTGCGCGGCTGCGGGAGCCCTG TGGATCTACCCCACCAATTGCCCCGTGCGCGACTACCAGCTGGACATCTCCCGGAGCGCGCTGTTCTGCA ACACCCTGGTGTGCCTGCCCACCGGGCTGGGGAAGACCTTCATTGCCGCCGTGGTCATGTATAATTTCTA CCGCTGGTTCCCTTCTGGCAAGGTGGTTTTCATGGCACCCACGAAGCCCCTGGTAACACAGCAGATGGAA GCCTGCTTCCACGTGATGGGTATTCCACAGTCTCACATGGCTGAAATGACAGGTTCAACTCAAGCTGTTA ACAGGAAGGAGATATGGTCCAGCAGGAGGGTTCTTTTCCTGACACCGCAGGTCATGGTAAATGACCTTAC TAGAGGGGCTGTTCCTGCCACCCACGTAAAGTGTCTTGTGGTGGATGAAGCTCATAAGGCACTTGGGAAC TATGCTTACTGCCAGGTGGTAAGAGAATTGGTCAAATACACCACTCACTTTCGTATCTTGGCTCTTAGTG CCACGCCAGGTAGTGATATCAAGGCTGTGCAACAGGTGATCACTAACCTACTAATTGGGAAGATAGAGCT TCGTTCGGAAGAGTCTCCCGATATTTTGCCTTACTCTCATGAAAGAAGAGTTGAGAAGCTCGTTGTCCCC CTTGGGGAAGAACTAGGAGCCATTCAAAAGACGTATATCCAGATTTTGGAAACATTTGCCAGTTCTTTGA TTCATAGAAATGTTTTGATGAAAAGAGACATTCCCAATCTAACAAAATACCAGATAATTCTGGCAAGAGA TCAATTTAGGAAAAACCCATCCCCAAATATTGTGGGAATTCAGCAAGGCATAATTGAAGGGGAGTTTGCC CTCTGCATTAGTTTATATCATGGTTATGAGTTACTGCAGCAAATGGGAATGCGGTCATTGTATTTCTTTC TTTCTGGAATCATGGATGGAACTAAAGGAATGACACGGGCAAGAAATGAGCTCAGCCGAAATGAGGACTT CATGAAGCTGTACACTCACCTGCAGAGCGCGTTTGCACCAGCCAGCACCAGCGATGCTTCGGCTTTCCAG AGAGGAAATAAAGAGAAAAAATTTGTCTATAGTCATCCAAAGTTAAAGAAACTGGAAGAAGTTATACTGG AACACTTCAAGTCATGGAATGCTAAAGCCACCACTGAGAAGAAGTGCCACGAGAGCAGAGTCATGATCTT CTCGTCATTCCGAGATAGCGTTGAGGAGATTGCAGAAATGCTTTTACAGCACCGGCCAGTTATTAGAGTG ATGACCTTTGTTGGCCATGCTTCAGGGAAAAACACAAAAGGTTTTACCCAGAAGGAACAACTGCAGGTGG TGAGACAGTTCCGAGACGGTGGCTATAACACACTGGTTTCCACCTGTGTTGGAGAAGAAGGTTTGGATAT AGGGGAAGTGGATCTTATAATATGCTTTGATGCCCAGAAGAGCCCAATCCGCCTCATCCAGCGCATGGGT AGAACTGGCCGCAAACGGCAAGGCAGGATTGTCGTTATCCTCGCTGAAGGACGGGAAGAGCGTACATATA ATCAGAGTCAGTCCAACAAAAAAAATATTTATAAAGCCATTTCAGGTAACAGACAAGTGCTGCGTTTATA CCAAGGAAGTCCACGAATGGTTCCTGATAAAATCAATCCTGAATTACACAAAATGTATATCACACATGGT GTCTATGAACCAGAGAAGGCTCGGAGTGTACAGCGAAGGCCGTTTTCCTCAAGGGGTGGAATAAAAGCTA GTAAATCGAATAAAGATGGGCTGTTATCAGAAGAGGAATTTAACCTATGGAGCAGACTCTATAGGTTAGG AGACAGTGACCAGGTTAAAGGAGTGGCATTGCCTCAGTCTCACTTCCCATCTTTACAGGAGGACAGAGTG ATTCAAGACCCAACTACTAGAATTCACCAACTCTCTCTCTCTGAATGGAGTCTCTGGCAAGATCGTCCGC TTCCTACACATCAAGTTGACCATTCAGATCGTTGCCACCATTTTATAAGCATTATGAAAATGATAGAAGG AATGAGACACGAAGAGGGTGAATGCAGCTATGAGTTGAAAATTAGACCTTTTCTACAAATGGAAGATGTC TGCTCAAAATATCACGCTCCTAGGAATGGATATAATAATGTAGCCAGTGTCGCCAGTAGTGCTCACCAGA AGTCTTCATTTAGACCGAGTGTAGATGCAGGAGGCTCCTTGACGGTGATAGAGTCTGAGGAAGAGCATGC TGACACTGTTAAACAAAGGGATTCTAAATGGACCAAGATTACTTCTCTAAGGGAAAAACCCTGTAGAGCA GGAAGAAAAGGCCAGACCTGTGAGCACAGTGAGGGTGAGGGTGAGGATGGGGATGCAGGTTCTTCCGATG CTGATGGACAGTCTCCTGCCGAAGCTGATTCCCAGGTAGACCCACCCAGTGGTGAAAGGATGGCAGATGT AGGTGGGATCTCCATCTTGGGTGCTGTCACTGAAGAGGATAACCATCCAGGAACATTACAAATGGAGTGT CAGGTCACAAATAAATCATGTGCCAGGTACTCATTAGATTCTGGTTATAGTAGTTTCAGTGATGAGAAAT CTGTATCATCAAATTTGTTTCTTCCATTGGAAGAAGAGCTGTTCACCGATCGAGCTGCTGAGCAGTTTTA TAATTGTCGGCCCATGACAGAAGACGTACTAGCTAATGTGGAGAGATTTTTATCCCGTTCTCCCCCGTCT CTCAGTGGACTCTCAGACTTGGTATATGATGTTACTCAGGGTTGTGAATTTGATAATGCATCCTGTTCAC CCTACCCAGAGCATGAACACAGTCCTAGACCTGTTTCTCCAGCGTCACACTCTGCTGGGAATTCTCAACA AAACTTAGAATCGAATTCAGCTAAACGTATTAGTCATCCTACTGAAAAATACTGTCTCCCTGGCACCACT CATAATAAGGTTTCTGATCGGCCCTCCTTCTGTGAGTCTGATAGCGAAGGCCATAATATTAAATATCAAA ATTCAGGATCTAACAGTTGTGCTCAAATACAAGCAGACCTTGAAAATAACTTCGTTGACAAGAACAGCCA TGATGATAGTGAACCCCCAGTATTGTTCACTGATGAAGATGAAAGCTTACTATTATTTGAAGATGATTTT AAGAATATTGAAGATGGTCCTGAAGAGTTAAATGGGGCGAGCCTTCCTCCCTTCAACAGTATCAGTCAGC CCCTCCGTGTGTCAGGTAAAACTCTCACAAGTGAAATGCCTCCAGTCTCTCACTTCTTAATTTCTGATGA ACTGTTGTTAGATGATGATTCTGAACCTGAAGACCAAATCGTCTGTGGTGCCAAGAGTTGGAAATGTCAG GAAGGGGTGGAGGATGGACAAGAAGAGCTGAGGACTGACGGACAGACTTTTGATTGCTCTGTAGACTTAT TTTCTGTTACCTTTGACTTAGGATTTCGTTGTAGTTCGGGTTCAGATGATGAAATGCTAGCAGGTGCATC TGATAGGACTCGCACCCTGGGAGCGGCCGATGTGTCTGGAAGGCATTCTGATAAGGAGATAAAGGATGCC GGTGGCGCTTCAGGTCCACTGGGTAGAGCCATATCACCAATCCCAACTGAGACAGCGCAGTGGTCGCCTT GGGCACAGAACAAGGAATATGCTTCTTTCCACGTTGCCTCATCCTCGCCAGTGAAGCAGAGAGTCAGGAG CACACCGCTGTCTAAGTCACACGCGAGCTCCAAGACAGGAGCACACATGCTCAAGACACTAGACTCTACC AAGGAGAAAGCAGGTGGACAGGGCTTCAAAATGGCATTGAACCCAAGACTGGGCCATCTAGGATTTTCTG TAGAAGAGACCAAGAGCAGCGATCAGGTCTTTGTGCATCAGTCCCCTCGGCGCACTGAAGTTGAACACTT AACTAGTGAAAGCGAAGATGATGTTTTCCTGAGAAAAACTAAAAAACCAAAAAGAAATGTCCTAAAGTCC CCGGAGGATCAGAAAAACAACGAAGTTGACTCACCCATTCATGCTGTAAAAAAACCCAGAGTTCTCAGGT CAGAATTAGCATCTAGCGATGATGAGAGTGAGAATTTTGGCAGAACGTGTCCAAGATTGGAACACTTCAA GGGTCGTAACAGGAACATCAGAAAAGGCTCCGCAGCCCAAAAGAACCGGAGTCAAGTAAAGACTATGGCT CGGAGGTTTTTAGACGACGAGGCAGAGGTTTCTGGAGAGGATGTGGACTGTGTCTCAGCAGATGAGGAGG ATGAGTCTGAGAACGAGCAGGATTCCTCCCTGCTCGACTTTGTAAATGACCGGACCCAGCTTTCACAGGC TATAAATGATTCTGAAATGAGAGCTATTTACATGAAGTCTGTACGCAGTCCATTGATGAGCACTAAGTAC AGAATGGTCCGTGAGAAGCGTCCCAACATGAACATTTTCTCACAGATTCCTGAGCAAGATGAAAACTATT TAGAGGACAGTTTTTGTGTTGATGAAGAAGAGTCTTGCAAAAGCCAATCAAGTGAAGAAGAAATTTCTGT TGATTTTAACTTAACTAAAGATTCCTTTACAGATGAGGACATACGATATAAAACTCGGTATGCAGTAAAG ATCAAACAAATGAACAAGAAACAAAACTACACACGGCCAAGAAAGAAATTGTCTAGAATTATCTTACCCG ACGATGACTCTAGTGAGGAAGAAAACATCCCGAAGGACAGAGAACACTCCGTTGCTGGTGGCCACGCTGC TGCTGAGCACACACAGCAGGGCCAGCTCTGGGCTTCAGGGCCATCCGGGTCCTCAGTGCCTCCCCAGGTC CTTTCTGATCCATCGTGGAATCAGTCATCCAGGCAGAGACTACAGGTGCAACCCAGTATAACAGATGCCG TGCCTAGAACCTTGAACGTGAAGGCTCAGAGCCATAATAAAATCAAATCTGCTTCACCACCATGTACTGG TGTTGAATCAAGGAAAGAATATGGGAACCATCCAGTTCAGTTGAAGGCTGACAGTCAGGAGCACAGTGAC ACCTCAGCAGCACCCTGTTCTACTTCACTCCTGCATGTGGCTGAGGGACATACAGCCCCTAGACATCTGC AAGAAGGAAACAGGGCTTGCATTCTTGTAGACAGTCGTGAAATCACTACCGGTTTGGAAGTAATCTCTTC GCTAAGAACAGTTCATGGGTTACAAGTAGAGATTTGTCCTCTTAATGGCTGTGACTACATCGTAAGCAGC CGAATGGTCGTAGTAAGGAGGTCTCAGTCTGAAATGTTAAGTAATACCAGCAAGAACAAGTTCATCGAGC AGATGCAGCGCCTGCAGAGCATGTTTCAAAGGATATGTGTGATTGTGGAAAAAGACAGAGAAAAAGCAGG AGACACATCAAAAAAATTCAGAAGAACGAAGTGCTATGACAGTTTGCTGACTGCCTTAGTTGGCGCCGGA ATCCGAATCCTTTTCAGCTCTGGACAAGAAGAAACTGCAGATTTACTAAAGGAGCTGTCATTGGTGGAAC AAAGAAAGAATGCTGGTATTCATATCCCAGCAGTGCTAAACACTAGTAAACTTGAAGCACTTCCATTCTA TTTAAGTATTCCTGGTATAAGTTACATCACAGCCTTAAATATGTGTCACCAGTTTTCATCTGTGAAAAAG ATGGCTAACAGCTCCCCTGAAGAAATCTCCACATGTGCGCAAGTAAATCATCAAAAGGCTGAAGAGATCT ATAAATATATTCACTATATATTTGACATGCAAATGTTACCAAATGATCTTAACCAGGAAAGACAAAAACC TGATACATGCTTAACACTTGGAGTCGCTATGAAAGAACTCTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_178912
Insert Size	6066 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_178912.3, NP_849243.2
RefSeq Size	7775 bp
RefSeq ORF	6066 bp
Locus ID	104806
UniProt ID	Q8BGE5
Gene Summary	DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates. In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA.[UniProtKB/Swiss-Prot Function]

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