Dscam (NM_031174) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4932410A21Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225120 representing NM_031174 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGAGCCCCACTCCAGCC TCTACTTTGTCAATGCATCGCTGCAAGAGGTAGTGTTTGCAAGCACATCGGGGACGCTGGTGCCCTGCCC GGCTGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCAACGGGCGAGGAGATCTACGATGTCCCC GGGATCCGCCACGTCCATCCCAATGGCACTCTCCAAATTTTCCCCTTTCCTCCTTCAAGCTTCAGCACCT TAATCCATGATAATACTTACTATTGCACAGCTGAAAACCCTTCAGGGAAAATTAGAAGTCAGGATGTCCA CATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTC GCGGTGTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACGTCACTGTCGTCTCATGGGAGAAAGACA CGGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAAGATGTACA GAACGAAGATGGGCTGTACAACTACCGCTGCATCACGCGGCACAGATACACAGGGGAGACGAGACAAAGC AACAGCGCGAGACTGTTCGTGTCAGACCCAGCAAACTCAGCCCCATCCATCCTGGACGGGTTTGACCACC GCAAAGCCATGGCAGGGCAGCGCGTGGAGCTGCCTTGCAAAGCACTTGGGCACCCCGAGCCAGACTACCG CTGGCTGAAGGACAACATGCCCCTGGAACTTTCTGGAAGGTTCCAAAAGACAGTGACTGGGTTGCTCATT GAGAACAGCCGCCCCTCAGACTCAGGCAGCTATGTGTGTGAAGTATCCAACCGATATGGCACTGCCAAGG TGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGAAAGGTTAAAAGCAGCGT GGGCAGCCAGGTCTCCTTATCCTGCAGTGTGACAGGAAATGAAGACCAGGAACTCTCCTGGTACCGAAAT GGCGAAATCCTCAACCCTGGAAAAAACGTGAGGATCACAGGACTCAACCACGCAAACCTTATAATGGATC ACATGGTCAAGAGTGATGGGGGTGCCTACCAGTGCTTTGTGCGCAAGGACAAGCTATCTGCTCAAGACTA TGTCCAGGTGGTCCTTGAAGACGGAACTCCCAAAATCATTTCTGCCTTTAGCGAGAAAGTGGTGAGCCCG GCAGAGCCAGTGTCCCTCGTGTGCAATGTGAAGGGTACACCCTTGCCCACGGTCACCTGGACCCTGGACG ATGACCCCATCCTCAAGGGCAGCGGTCACCGCATCAGCCAAATGATCACGTCCGAGGGGAACGTGGTCAG CTACCTGAATATCTCCAGCTCCCAGGTCCGGGATGGGGGTGTCTACCGCTGCACTGCCAACAACTCGGCT GGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCTGCAAGCATCAGACCAATGAAAAACATCA CTGCGATAGCGGGGCGTGACACGTACATCCACTGCCGCGTAATTGGCTATCCGTATTACTCCATCAAGTG GTACAAGAACGCTAACCTGCTTCCTTTCAACCACCGCCAGGTGGCGTTTGAGAACAATGGGACTCTGAAA CTCTCTGATGTGCAGAAAGAAGTTGACGAGGGAGAGTACACGTGTAATGTGCTGGTACAGCCACAGCTCT CCACCAGCCAGAGTGTCCACGTGACAGTGAAAGTTCCACCTTTCATCCAACCTTTTGAGTTCCCAAGATT CTCCATCGGGCAGCGGGTTTTCATCCCATGTGTGGTGGTCTCAGGGGACTTACCCATCACCATCACCTGG CAGAAGGATGGCCGGCCAATTCCAGCAAGCCTCGGAGTAACCATTGACAACATTGACTTCACCAGCTCCC TGAGGATCTCCAACCTCTCCCTAATGCACAATGGGAATTACACCTGCATTGCGAGAAACGAGGCAGCAGC CGTGGAACACCAGAGTCAGCTGATTGTGAGAGTTCCCCCTAAGTTCGTGGTACAGCCCCGGGACCAGGAC GGGATCTATGGCAAAGCAGTGATTCTCAATTGCTCTGCAGAGGGTTATCCTGTGCCTACAATTGTGTGGA AATTCTCAAAAGGTGCTGGGGTCCCCCAGTTCCAGCCAATTGCCTTGAATGGCCGAATCCAGGTCCTGAG TAATGGCTCACTCTTGATCAAGCATGTTGTAGAAGAAGACAGTGGCTACTACCTCTGCAAGGTCAGCAAC GATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTCACAGTGAAAATTCCTGCCATGATAACCTCTTACC CCAACACCACCCTGGCCACTCAGGGTCAAAGGAAGGAAATGAGCTGCACAGCCCATGGGGAGAAGCCCAT CATTGTCCGCTGGGAGAAAGAGGACAGGATCATTAACCCTGAAATGGCCCGCTACCTGGTATCCACCAAG GAGGTGGGAGAGGAAGTGATATCTACGCTGCAGATTTTGCCAACAGTGAGAGAAGATTCCGGTTTCTTCT CCTGCCATGCTATCAATTCATACGGGGAGGACCGTGGAATAATTCAACTCACAGTGCAAGAACCCCCAGA TCCTCCCGAGATTGAGATCAAAGATGTCAAAGCTCGCACCATCACGCTCAGGTGGACCATGGGGTTTGAT GGCAACAGCCCCATCACAGGCTATGACATTGAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAAA GAACCAAAGATGTTTCCCCTCAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAG CATCCGCATGTACGCCAAGAACCGGATTGGCAAGAGTGAGCCCAGCAACGAGATCACCATCACGGCGGAT GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTTCACTTGGAACCCACCTCATCTCAGAGTATCAGGGTTA CCTGGAAGGCTCCCAAGAAACACTTACAAAACGGGATCATTCGCGGCTACCAAATAGGCTACCGGGAGTA CAGCACGGGGGGTAACTTCCAGTTCAACATCATCAGTATCGACACCACCGGGGACAGCGAAGTGTACACC CTGGACAACCTGAATAAGTTCACGCAGTATGGCCTGGTAGTACAGGCTTGCAACCGGGCCGGCACAGGAC CTTCTTCTCAGGAGATCATTACCACCACTCTGGAGGATGTACCCAGCTACCCTCCTGAAAATGTCCAAGC CATAGCAACCTCACCAGAAAGCATATCAATCTCCTGGTCCACACTGTCCAAGGAGGCCTTGAATGGGATT CTCCAGGGGTTCAGGGTCATCTACTGGGCCAACCTCATAGACGGAGAGCTGGGCGAGATTAAGAACGTCA CCACCACGCAGCCTTCCTTGGAGTTGGATGGACTAGAGAAATACACCAACTACAGTATCCAGGTCCTGGC CTTCACCCGTGCAGGGGATGGCGTCCGGAGCGAGCAGATCTTTACCCGTACCAAGGAGGACGTTCCAGGT CCTCCTGCCGGTGTCAAGGCGGCGGCAGCCTCGGCCTCCATGGTCTTCGTGTCCTGGCTGCCCCCGCTGA AGCTGAACGGCATCATTCGGAAGTACACAGTGTTCTGCTCCCATCCCTACCCTACCGTCATCAGTGAGTT TGAAGCCTCCCCTGACTCATTTTCCTACAGAATCCCTAATCTGAGTCGGAATCGGCAGTATAGCGTCTGG GTGGTGGCGGTGACTTCCGCCGGAAGAGGCAACAGCAGCGAGATCATCACTGTGGAGCCCCTAGCTAAAG CTCCTGCACGGATCCTCACGTTCAGCGGGACAGTGACTACTCCATGGATGAAGGACATTGTCTTGCCTTG TAAAGCTGTTGGAGACCCGTCACCTGCCGTAAAATGGATGAAGGATAGTAACGGGACACCCAGCCTGGTG ACGATTGATGGCCGCAGGAGCATCTTCAGCAATGGGAGTTTCATCATTCGGACGGTGAAAGCAGAGGACT CTGGCTATTACAGCTGTGTTGCCAATAACAACTGGGGATCAGATGAGATCATATTAAATTTGCAAGTACA AGTTCCACCAGATCAGCCTCGGCTCACCGTATCCAAGACGACATCCTCCTCCATCACCCTCTCCTGGCTC CCTGGAGATAATGGGGGTAGCTCCATTAGAGGCTACATCCTGCAATACTCCGAGGATAACAGTGAGCAGT GGGGCAGCTTTCCCATCAGCCCCAGCGAGCGTTCATACCGCTTGGAAAACCTAAAGTGTGGGACTTGGTA TAAGTTCACCCTTACTGCCCAAAATGGAGTAGGTCCCGGGCGCATAAGTGAAATCATAGAAGCCAAAACC CTGGGGAAAGAACCCCAGTTCTCCAAGGAGCAGGAGCTCTTCGCCAGCATCAATACCACCCGAGTGAGGC TGAATCTGATTGGCTGGAATGACGGCGGCTGTCCAATCACCTCATTCACTCTTGAATACAGACCCTTTGG GACCACGGTCTGGACCACAGCTCAGCGGACCTCCCTTTCCAAGTCCTACATTCTGTATGACCTGCAAGAA GCCACGTGGTATGAACTGCAGATGAGAGTGTGCAACAGCGCCGGCTGTGCGGAGAAGCAAGCCAACTTCG CCACGCTGAACTACGATGGCAGTACAATCCCTCCACTCATTAAGTCAGTTGTCCAAAGCGAAGAAGGGCT GACAACCAACGAAGGGCTCAAGATCCTCGTGACCATCTCCTGCATCCTGGTCGGGGTTCTACTGCTCTTT GTGCTTCTGCTGGTTGTGCGGAGGAGACGGCGAGAGCAGAGGCTGAAGAGGCTGAGAGATGCAAAGAGTT TAGCTGAAATGCTCATGAGCAAAAACACACGGACTTCAGATACCTTAAGCAAACAGCAGCAGACTTTGAG AATGCACATTGATATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACAATGGAGACCATAGATGAC CGCTCCACAGTCCTGTTGACGGATGCTGACTTCGGGGAGGCAGCCAAACAGAAGTCACTGACAGTGACTC ACACGGTGCATTACCAATCGGTGTCTCAGGCCACCGGGCCCCTCGTGGATGTCTCCGATGCTCGGCCAGG AACGAATCCCACCACCAGGAGGAATGCAAAGGCTGGACCCACAGCGAGAAACCGGTACGCCAGCCAGTGG ACGCTCAACAGACCCCATCCTACCATCTCTGCACACACCCTCACCACAGACTGGAGACTGCCTACACCCA GGGCTACAGGATCCGTGGACAAGGAGAGCGACAGCTACAGCGTCAGCCCATCACAAGACACAGACCGAGC AAGAAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCTACCTACGAAGAACTGGCCAGGGCCTATGAACAC GCCAAGATGGAAGAGCAGCTGAGGCATGCCAAGTTCACCATCACAGAGTGCTTCATATCCGATACGTCCT CCGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCCAGTACCCCTTCAGAATCGGG GATCTGCAGATTCACTGCATCTCCCCCCAAACCTCAGGATGGAGGACGAGTGGTGAACATGGCGGTTCCA AAGGCCCATCGGCCAGGCGACCTCATACACCTGCCTCCATACCTACGAATGGACTTCTTGTTAAACCGGG GCGCACCAGGCACCAGCAGGGACCTGAGTTTAGGACAAGCGTGCTTGGAACCCCAGAAAAGTCGGACCCT GAAACGCCCCACGGTCCTTGAGCCCACCCCTATGGAGGCCTCCTCCTCCACCTCTTCCACGCGAGAAGGA CAGCAGTCGTGGCAACAAGGGGCTGTGGCCACCTTACCTCAGCGAGAGGGTGCAGAGCTGGGACAGGCAG CTAAAATGAGCAGCTCCCAAGAGTCACTGCTGGACTCCCGGGGCCATTTGAAAGGAAACAATCCCTACGC AAAATCTTACACCTTGGTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031174
Insert Size	6042 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031174.4, NP_112451.1
RefSeq Size	7481 bp
RefSeq ORF	6042 bp
Locus ID	13508
UniProt ID	Q9ERC8
Gene Summary	Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed:18216855, PubMed:19196994, PubMed:19945391). Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC (PubMed:18585357). Might also collaborate with UNC5C in NTN1-mediated axon repulsion independently of DCC (PubMed:22685302). In spinal cord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).[UniProtKB/Swiss-Prot Function]

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