Myh14 (NM_028021) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2400004E04Rik; II-C; NHMCII; NMHC II-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225105 representing NM_028021 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGTGACCATGTCCGTGTCTGGGAGGAAGGTAGCCTCCAGGCCAGGCCCGGTGCCTGAGGCAG CCCAATCGTTCCTCTACGCGCCCCGGACGCCAAATGTAGGTGGCCCTGGAGGGCCACAGGTGGAGTGGAC AGCCCGGCGCATGGTGTGGGTGCCCTCGGAACTGCATGGGTTCGAGGCAGCAGCCCTGCGGGATGAAGGG GAGGAGGAGGCAGAAGTGGAGCTGGCGGAGAGTGGGCGCCGCCTGCGGCTGCCCAGGGACCAGATCCAGC GCATGAACCCACCCAAGTTCAGCAAGGCAGAAGATATGGCTGAGCTCACCTGCCTCAACGAGGCCTCGGT CCTGCACAACCTGCGAGAACGCTACTACTCCGGGCTCATTTATACCTACTCTGGCCTCTTCTGTGTGGTC ATTAACCCATACAAGCAGCTGCCCATCTACACGGAGGCCATTGTTGAAATGTACCGGGGCAAGAAGCGCC ATGAGGTGCCACCTCACGTGTATGCTGTGACGGAGGGCGCGTACCGCAGCATGCTTCAGGATCGTGAGGA TCAATCCATTCTCTGCACGGGAGAGTCTGGCGCTGGGAAGACGGAGAACACCAAGAAGGTCATCCAGTAC CTGGCCCATGTGGCATCATCTCCAAAGGGCAGGAAGGAGCCTGGTGTCCCTGGGGAGCTAGAGCGTCAGC TTCTTCAAGCCAACCCCATCCTAGAAGCCTTTGGCAATGCCAAGACGGTGAAGAACGACAACTCTTCCCG ATTTGGCAAATTCATCCGCATCAACTTTGATATTGCTGGCTACATCGTGGGAGCAAACATCGAGACCTAT CTGTTGGAGAAGTCCCGGGCCATCAGACAGGCCAAGGATGAATGCAGCTTCCATATCTTCTACCAGCTGC TAGGGGGCGCTGGGGAGCAGCTAAAAGCTGACCTCCTTCTGGAGCCCTGTTCCCATTATCGCTTCCTGAC CAATGGGCCCTCATCGTCCCCGGGCCAGGAGCGTGAGTTATTCCAGGAGACCCTGGAGTCCCTGCGTGTG CTGGGCCTCCTCCCAGAAGAGATCACTGCCATGCTGCGCACTGTCTCTGCTGTCCTCCAGTTTGGCAACA TTGTCCTGAAGAAAGAGCGCAATACGGACCAAGCCACCATGCCTGACAACACAGCTGCCCAGAAGCTTTG CCGCCTCTTGGGACTCGGAGTGACCGACTTCTCCAGAGCCCTTCTCACACCCCGCATCAAAGTGGGCCGA GATTATGTTCAGAAAGCACAAACCAAGGAGCAGGCTGACTTTGCGCTGGAGGCTCTGGCCAAAGCTACCT ATGAGCGCCTGTTCCGCTGGCTGGTTCTGCGGCTCAACCGTGCCCTGGACAGAAGCCCGCGGCAGGGTGC CTCCTTCCTGGGCATCCTGGACATCGCGGGCTTTGAGATCTTCCAGCTGAACTCCTTCGAGCAGCTGTGC ATCAACTACACCAACGAGAAGCTACAGCAGCTATTCAACCACACCATGTTCGTGCTGGAGCAGGAGGAGT ACCAGCGAGAGGGCATCCCCTGGACCTTCCTAGACTTCGGGTTGGACCTGCAACCTTGCATCGACCTCAT TGAGCGTCCGGCCAACCCTCCAGGTCTCCTGGCCCTGCTGGACGAGGAGTGCTGGTTCCCCAAGGCCACG GACAAGTCTTTTGTGGAGAAGGTTGCCCAGGAGCAGGGCAGCCACCCCAAGTTCCAGCGCCCCAGGAACC TGCGAGATCAGGCCGACTTCAGCGTCCTGCACTATGCCGGCAAGGTTGACTACAAAGCCAGTGAGTGGCT GATGAAGAACATGGACCCACTGAATGACAATGTGGCCGCCTTGCTTCACCAGAGCACGGATCGTCTCACA GCTGAGATCTGGAAGGATGTGGAGGGCATCGTGGGGCTGGAGCAAGTAAGCAGCCTTGGAGATGGCCCAC CGGGAGGCCGCCCCCGCCGTGGAATGTTCCGGACTGTGGGGCAGCTCTACAAAGAATCCCTGAGCCGCCT CATGGCCACGCTCAGCAACACCAACCCTAGTTTTGTCCGCTGCATCGTTCCCAATCATGAGAAGAGGGCT GGAAAGCTGGAGCCGCGCCTGGTGCTGGACCAACTCCGTTGTAACGGGGTCCTCGAGGGTATCCGCATCT GTCGCCAAGGCTTCCCCAACCGCATCCTCTTCCAGGAGTTCCGACAGCGCTATGAAATCCTCACCCCGAA CGCTATTCCCAAGGGCTTCATGGACGGCAAACAGGCCTGTGAGAAGATGATCCAGGCCCTGGAGCTAGAC CCCAACCTGTACCGTGTTGGCCAAAGCAAGATCTTCTTCCGGGCAGGGGTCCTGGCCCAGCTGGAGGAGG AGCGGGACCTGAAAGTCACCGACATCATAGTGTCTTTCCAGGCAGCGGCACGGGGCTACCTGGCCCGTAG GGCTTTCCAGAGACGGCAGCAGCAGCAGAGTGCTCTGAGGGTGATGCAGAGAAACTGTGCTGCCTACCTC AAGCTCAGGAACTGGCAGTGGTGGAGGCTGTTCATCAAGGTGAAGCCCCTGCTGCAGGTGACACGGCAGG ATGAGGTGCTGCAGGCGCGCGCCCAGGAGCTGCAGAAAGTTCAGGAGCTGCAGCAGCAGAGCGCTCGTGA AGTGGGGGAACTGCAGGGTCGAGTGGCACAGCTAGAGGAGGAGCGCACGCGCCTGGCTGAGCAGCTTCGA GCAGAAGCCGAGCTCTGCTCTGAGGCCGAGGAGACGCGGGCGCGACTGGCTGCCCGGAAGCAGGAGCTGG AGCTGGTGGTGACAGAGCTGGAGGCACGAGTGGGCGAGGAAGAAGAGTGCAGCCGGCAGCTGCAGAGTGA GAAGAAGAGGCTGCAGCAGCATATCCAGGAGCTAGAGAGCCACCTGGAAGCTGAGGAGGGTGCCCGGCAG AAGCTACAGCTGGAGAAGGTGACCACAGAGGCCAAGATGAAGAAATTTGAGGAGGACCTGCTGCTCCTGG AGGACCAGAATTCCAAGCTGAGCAAGGAGCGGAGGCTGCTGGAGGAGCGGCTGGCTGAGTTCTCCTCACA GGCAGCAGAAGAGGAAGAGAAAGTCAAAAGTCTCAACAAGCTGAGGCTCAAATATGAAGCCACAATCTCA GACATGGAAGACCGGCTGAAGAAGGAGGAGAAGGGACGCCAGGAACTAGAGAAGCTGAAGCGACGGCTGG ACGGGGAGAGCTCAGAGCTTCAGGAGCAGATGGTGGAGCAGAAGCAGAGGGCAGAGGAACTGCTCGCACA GCTGGGCCGCAAGGAGGATGAGCTGCAGGCCGCCCTGCTCAGGGCAGAGGAAGAGGGTGGTGCCCGTGCC CAGTTGCTCAAGTCCCTGCGAGAGGCACAGGCTGGCCTTGCTGAGGCTCAGGAGGACCTGGAAGCTGAGC GGGTAGCCAGGGCCAAGGCGGAGAAGCAGCGCCGGGACCTGGGCGAGGAGTTGGAGGCCCTACGTGGGGA GCTCGAGGACACTCTGGATTCCACCAACGCCCAGCAGGAGCTGCGGTCCAAGAGGGAGCAGGAGGTGACA GAGCTGAAGAAAGCATTGGAAGAGGAGTCCCGTGCCCATGAGGTGTCCATGCAGGAGCTGAGACAGAGGC ATAGCCAGGCACTGGTGGAGATGGCCGAGCAGTTGGAGCAAGCCCGGAGGGGCAAAGGTGTGTGGGAGAA GACTCGGCTATCCCTGGAGGCTGAGGTGTCCGAGCTGAAGGCCGAGCTGAGCAGCCTGCAGACCTCGAGA CAGGAGGGTGAGCAGAAGAGGCGCCGCCTGGAGTCCCAGCTACAGGAGGTCCAGGGCCGATCCAGTGATT CGGAGCGGGCTCGGTCTGAGGCTGCTGAGAAGCTGCAGAGAGCCCAGGCGGAACTTGAGAGCGTGTCCAC AGCCCTGAGTGAGGCGGAGTCCAAAGCCATCAGGCTGGGCAAGGAGCTGAGCAGTGCAGAGTCCCAGCTG CATGACACCCAGGAACTGCTTCAGGAGGAGACCAGGGCAAAGCTGGCCTTGGGGTCCCGTGTGCGTGCCC TAGAGGCCGAGGCGGCGGGGCTTCGGGAGCAGATGGAAGAGGAGGTGGTTGCCAGGGAACGGGCTGGCCG GGAGCTGCAGAGCACGCAGGCCCAGCTCTCTGAATGGCGGCGCCGCCAGGAAGAAGAGGCCGCGGTGCTG GAGGCTGGGGAGGAGGCTCGGCGCCGTGCAGCCCGGGAGGCAGAGACCCTGACCCAGCGCCTGGCAGAAA AGACTGAGGCTGTAGAACGACTGGAGCGAGCCCGGCGCCGACTGCAGCAGGAGTTGGACGATGCCACTGT GGATCTGGGGCAGCAGAAGCAGCTCCTGAGCACACTGGAGAAGAAGCAGCGGAAATTTGACCAGCTCCTG GCAGAGGAGAAGGCTGCAGTTCTACGGGCTGTGGAAGACCGTGAACGGATAGAGGCCGAAGGCCGGGAGC GAGAGGCCCGGGCCCTGTCGCTGACCCGGGCCCTGGAAGAGGAGCAGGAGGCCCGGGAGGAGCTGGAGAG GCAGAACCGTGCTCTGAGGGCTGAGCTGGAAGCACTGCTGAGCAGCAAGGATGACGTGGGCAAGAACGTG CACGAGCTGGAGCGAGCCCGTAAGGCGGCTGAACAGGCAGCCAGTGACCTGCGGACACAGGTGACAGAAT TGGAGGATGAGCTGACAGCCGCAGAGGATGCCAAGCTGCGCCTGGAGGTGACTGTGCAGGCTCTGAAGGC TCAACATGAACGCGACCTGCAGGGCCGCGATGATGCCGGTGAGGAGAGGCGGAGGCAGCTGGCCAAGCAG CTAAGAGACGCAGAGGTAGAGCGCGATGAGGAACGGAAGCAGAGGGCACTGGCTATGGCTGCCCGCAAGA AGCTGGAGCTGGAACTGGAGGAGTTGAAGGCGCAGACATCTGCTGCTGGGCAGGGCAAGGAAGAGGCAGT GAAGCAGCTGAAGAAGATGCAGGTCCAGATGAAGGAGCTGTGGCGGGAGGTAGAGGAGACGCGTAGCTCC CGCGACGAGATGTTTACCCTGAGCAGGGAAAATGAGAAGAAGCTCAAGGGGCTGGAAGCTGAGGTGCTGC GTCTGCAAGAGGAACTTGCTGCCTCAGACCGAGCCCGGAGGCAGGCCCAGCAAGACAGAGACGAGATGGC AGAGGAGGTGGCCAGTGGCAATCTTAGCAAGGCAGCCACCCTGGAGGAAAAACGGCAGCTGGAGGGGCGA CTGAGCCAGTTGGAAGAGGAGCTGGAGGAAGAACAGAACAACTCGGAGCTGCTCAAGGACCATTACCGAA AGCTAGTGCTACAGGTCGAGTCCCTCACCACAGAACTGTCTGCCGAACGAAGTTTCTCAGCCAAGGCCGA GAGTGGACGGCAGCAGCTGGAGCGGCAGATCCAGGAACTGCGGGCCCGCTTGGGTGAAGAGGATGCTGGA GCCCGAGCCAGGCAGAAAATGCTGATCGCTGCTCTGGAGTCTAAACTGGCCCAGGCAGAGGAGCAGCTGG AGCAGGAGAGCAGGGAGCGCATCCTCTCTGGCAAGCTGGTACGCAGAGCTGAGAAGCGGCTGAAGGAGGT AGTTCTTCAGGTGGATGAAGAGCGCAGGGTGGCTGACCAGGTCCGGGACCAGCTGGAGAAAAGCAACCTC CGGCTGAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGAGGAGGCATCTCGGGCACAGGCTGGTCGGA GGCGGCTGCAGCGGGAGCTGGAGGACGTCACTGAGTCTGCAGAATCCATGAACCGGGAGGTGACCACGCT GAGGAACAGGCTCCGGCGTGGCCCACTTACATTCACCACACGGACTGTGCGCCAGGTGTTCCGGCTGGAA GAGGGCGTGGCTTCTGACGAGGAAGAGGCTGAAGGAGCTGAACCTGGCTCTGCACCAGGCCAGGAGCCGG AGGCTCCGCCCCCTGCCACACCCCAATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_028021
Insert Size	5979 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_028021.3, NP_082297.1
RefSeq Size	6392 bp
RefSeq ORF	5979 bp
Locus ID	71960
UniProt ID	Q6URW6
Gene Summary	Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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