Dock4 (NM_172803) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5330406C03; 6330411N01Rik; AF263288; C030023J22; mKIAA0716
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225101 representing NM_172803 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACCTACGGAGCACGAGAAATACGGCGTGGTTATTGCCAGCTTTCGAGGCACCGTCCCGTACG GCCTGTCATTGGAGATCGGAGACACTGTTCAGATCCTGGAGAAGTGCGACGGCTGGTACAGAGGATTTGC CTTAAAAAATCCCAACATCAAGGGTATATTTCCTTCCAGCTATGTTCACTTGAAAAATGCCTGTGTAAAG AACAAAGGACAATTTGAAATGGTTATTCCCACTGAAGATTCTGTTATCACCGAGATGACATCAACTTTAA GGGACTGGGGAACCATGTGGAAACAGCTCTATGTCAGAAATGAAGGGGATCTCTTCCATCGCCTGTGGCA CATCATGAATGAAATCCTCGATCTGCGACGGCAGGTGCTAGTTGGCCATCTCACCCATGACAGGATGAAG GATGTGAAGCGCCACATTACTGCCCGACTGGATTGGGGAAATGAACAACTGGGACTAGACCTGGTGCCTA GGAAGGAGTACGCAATGGTAGACCCAGAGGACATCAGCATCACTGAGCTGTACCGACTGATGGAACATCG ACATCGGAAGAAAGACACCCCTGTGCAGGCCAGCAGTCACCACCTCTTTGTCCAGATGAAGAGCCTCATG TGTTCCAACCTGGGAGAGGAGCTAGAGGTCATCTTCTCACTCTTTGACAGCAAAGAGAATCGCCCAATCA GTGAGAGATTTTTCTTGAGACTGAATAGAAATGGGCTTCCCAAAGCCCCGGATAAGCCTGAAAGACACTG TTCTCTCTTTGTGGACTTGGGTAGCAGTGAACTCAGGAAGGACATCTACATTACCGTGCACATTATCCGA ATTGGTCGAATGGGAGCAGGAGAGAAAAAGAATGCCTGCAGTGTTCAGTACCGGCGACCTTTTGGCTGTG CAGTCCTTAGCATTGCAGATCTCCTCACCGGAGAGACAAAGGATGACCTGGTCCTGAAAGTGTACATGTG TAATACAGAGAGCGAGTGGTACCAGATCCACGAGAACATCATCAAGAAGCTGAATGCGCGCTATAATTTG ACTGGCTCCAATGCAGGATTAGCAGTTTCCCTCCAACTGTTACACGGAGACATTGAACAGATCAGAAGAG AATACTCCTCAGTGTTCTCTCACGGAGTGTCCATAACGCGGAAGCTGGGTTTTTCTGACATTATCATGCC TGGTGAAATGAGGAATGATTTATATATCACCGTAGAAAGGGGGGAATTTGAGAAAGGAGGCAAGAGTGTG GCCAGAAATGTGGAAGTTACAATGTTCATTGTAGACAGTAATGGTCAGCCCTTAAAGGATTTTATCTCCT TTGGCTCTGGAGAGCCACCGGCTAGTGAGTACCACTCCTTTGTGCTCTATCATAACAACAGTCCCAGGTG GTCAGAGCTGCTGAAACTTCCTATTCCTGTGGACAAATTCCGGGGCTCCCACATCCGCTTTGAGTTCCGG CATTGTTCCACAAAGGAGAAAGGAGAAAAGAAGTTGTTCGGTTTCTCTTTCGTGCCCCTGATGCAAGAAG ACGGAAGGACTCTCCCAGATGGCACACACGAGCTCATCGTGCATAAGTGTGAAGAAAACACAAACCTTCA GGATACTACCCGCTACCTCAAGCTCCCATTTTCCAAGGTCATCTTCCTTGGGAACAATAATCAAACCATG AAGGCCACAAAGGAATCCTTTTGGATAACATCATTTCTCTGTTCCACAAAACTCACTCAAAATGGTGATA TGCTTGATCTTTTGAAATGGAGAACCCACCCAGACAAGATCACAGGCTGTCTCTCTAAGTTAAAAGAAAT CGATGGCTCAGAGATAGTAAAGTTTCTCCAAGACACACTGGATACCTTGTTTGGAATATTAGATGAAAAT TCCCAAAAATATGGGTCTAAAGTGTTTGATTCTTTGGTCCACATAATAAATTTGCTACAAGATAGCAAGT TCCATCATTTTAAGCCTGTCATGGACACTTACATTGAGAGTCACTTTGCTGGGGCACTTGCATACAGAGA TCTCATCAAAGTGCTCAAGTGGTACGTGGACAGGATAACGGAAGCAGAGCGCCAGGAGCATATCCAGGAA GTGTTGAAGGCACAAGAGTACATTTTTAAGTATATAGTTCAATCCCGAAGGCTGTTTTCCCTTGCCACCG GAGGGCAGAATGAAGAGGAATTCCGCTGCTGCATCCAGGAGCTCCTTATGTCCGTCCGCTTCTTCCTCTC ACAAGAGAGCAAAGGGACTGGAGCTTTATCTCAGTCGCAGGCTGTGTTCTTGAGCTCCTTTCCAGCTGTG TACTCAGAACTGTTGAAGCTCTTTGATGTCCGGGAAGTGGCCAACTTGGTCCAGGACACCCTGGGCAGCC TACCTACCATCATGCATGTGGACGATTCTCTGCAGGCAATTAAGCTTCAGTGCATTGGCAAAACTGTCGA GAGCCAGCTCTATACCAACCCAGACTCGCGGTACATTCTCCTGCCTGTCGTACTGCATCACCTCCACATC CACTTGCAAGAACAGAAGGACTTGATCATGTGTGCACGTATCCTTAGCAACGTGTTCTGTCTCATCAAGA AAAATAGCTCAGAAAAGTCTGTGCTGGAGGAAATAGACGTGATAGTGGCCAGCTTGCTGGATATCCTGCT CAGGACCATATTGGAGATCACTAGTCGGCCTCAAGCATCCAGCTCCGCCATGAGGCTGCAGTTCCAGGAT GTCACTGGGGAATTTGTTGCCTGTCTCCTTTCCCTATTACGACAAATGACAGATAGACATTATCAGCAAC TTCTGAATAGTTTCAGTACTAAAGAGGAGCTGAGGGATTTCTTGCTGCAGATTTTTACCGTTTTCCGAAT ATTGATACGCCCAGAGATGTTTCCCAAGGACTGGACTGTCATGCGCCTGGTTGCTAACAATGTTATCATC ACAACAGTTCTGTACCTCTCCGATGCACTCCGTAAGAACTTCTTAAATGAAAACTTTGATTATAAGATCT GGGATTCCTACTTTTACCTCGCTGTCATTTTTATAAACCAGTTGTGTCTGCAGCTGGAGATGTTCACACC TTCTAAAAAGAAAAAGGTGCTAGAAAAGTATGGTGATATGCGCGTCACCATGGGTTGTGAAATTTTTAGC ATGTGGCAAAACCTAGGAGAACACAAACTTCATTTCATCCCTGCCCTGATTGGGCCCTTTCTGGAAGTAA CCTTGATACCCCAGCCAGATCTCCGGAATGTCATGATTCCTATTTTTCACGATATGATGGACTGGGAGCA AAGAAGGAGTGGCAACTTTAAACAGGTGGAGGCCAAGCTAATTGACAAATTGGACAGCCTGATGTCGGAG GGCAAAGGTGATGAAACGTACCGTGAGCTCTTCAACAGTATAATTCCACTATTTGGTCCCTACCCTAGTC TACTAAAGAAAATTGAGCGGGAAACATGGAGGGAAAGTGGCGTTTCATTAATTGCCACTGTCACTCGGCT GATGGAGAGGTTGTTAGACTACAGGGACTGCATGAAGATAGGAGAGGTGGATGGCAAGAAGATTGGCTGC ACAGTTAGCCTTTTGAACTTCTATAAGACGGAGCTGAACAAGGAGGAGATGTATATCCGCTACATCCACA AACTCTACGATCTGCATCTCAAGGCGCAGAACTTCACAGAGGCTGCCTACACTCTCCTGTTGTATGACGA GCTGCTGGAGTGGTCTGATCGGCCCCTCAGGGAGTTCCTGACCTACCCCATGCAGACCGAATGGCAGCGC AAGGAGCACCTGCACCTCACCATCATCCAGAACTTCGACCGAGGCAAATGTTGGGAGAATGGCATTATCT TGTGCCGGAAGATTGCAGAGCAATATGAGAGTTACTATGACTACAGGAACCTGAGCAAGATGAGGATGAT GGAAGCCTCCTTGTATGACAAAATCATGGACCAGCAGCGCCTGGAACCTGAATTCTTCAGAGTTGGATTT TATGGGAAAAAGTTTCCATTTTTCTTAAGAAATAAGGAGTTTGTATGTCGAGGGCATGACTATGAAAGAC TGGAGGCCTTCCAACAGCGAATGCTGAACGAGTTCCCCCATGCCATCGCCATGCAGCATGCCAACCAGCC CGACGAGACCATCTTCCAGGCAGAGGCTCAGTATTTACAGATATATGCTGTGACTCCCATTCCAGAGAGC CAGGAGGTACTGCAGAGAGAGGGGGTCCCAGACAACATCAAAAGCTTCTATAAAGTGAACCACATTTGGA AGTTCCGCTACGACAGGCCATTCCACAAAGGCGCAAAAGACAAGGAGAACGAATTCAAGAGTCTCTGGGT GGAAAGGACATCGCTGTACTTGGTCCAGAGCTTACCCGGCATCTCCCGCTGGTTCGAAGTGGAAAAGCGA GAAGTGGTAGAGATGAGTCCTCTGGAAAACGCCATCGAAGTGTTAGAAAACAAGAACCAGCAGCTGAAGA CCCTGATCAGCCAGTGTCAGACGAGGCAGATGCAGAATATCAACCCCTTGACCATGTGCCTCAATGGAGT CATAGATGCTGCAGTGAACGGTGGGGTCTCCAGGTATCAAGAGGCATTCTTTGTCAAAGACTATATTTTA AGTCATCCTGAAGATGGGGAGAAGATTGCACGGTTAAGAGAACTGATGCTTGAGCAGGCACAGATTCTGG AATTTGGGCTGGCCGTGCACGAGAAGTTTGTACCTCAAGATATGAGACCCCTTCACAAAAAACTGGTGGA CCAGTTCTTTGTGATGAAATCGAGCTTTGGGATACAGGAGTTCCCTGCTTGTATCCAGGCCAGCCCTGTC CATTTTCCCAATGGAAGCCCTCGTGTGTGTAGAAACTCAGCCCCTGCTTCCATGAGTCCAGATGGTACCA GGGTAATCCCTAGACGCAGCCCATTAAGTTACCCAGCTGTCAACCGGTATTCCTCGTCCTCGCTGTCCTC ACAGGCCTCTGCTGAAGTAAGCAATATTACAGGGCAATCAGAAAGCTCTGATGAAGTCTTTAACATGCAG CCCAGTCCGTCTACCTCAAGCCTGAGTTCCACTCACTCTGCTTCACCTAATGTGACCAGTTCTGCTCCGT CGAGTGCCAGAGCTTCGCCTTTGTTGTCTGACAAACACAAACATTCCAGAGAAAACTCTTGCCTGTCCCC AAGAGATAGACCGTGCAGTGCCATTTACCCAACACCCGTGGAGCCTTCTCAGAGGATGCTGTTTAATCAT ATTGGAGATGGGGCCTTGCCTCGAAGTGACCCCAACCTTTCTGCACCTGAGAAAGCTGTGAATCCCACCC CTAGCAGCTGGAGCCTGGACAGTGGGAAAGAAGCCAAGAACATGTCGGACAGTGGGAAACTTATCTCTCC CCCTGTCCCTCCAAGGCCCACACAGACTGCTTCGCCAGCAAGACACACCACATCAGTGTCCCCCTCGCCT GCTGGACGTTCTCCATTGAAGGGCTCCGTACAGTCCTTCACCCCATCTCCAGTGGAGTACAACTCCCCAG GACTGAGTTCAAATTCCCCAGTCTTGTCCGGCAGCTACAGCAGTGGGATTTCCTCGCTTAGCCGGTGTAG CACGTCGGAAACCTCGGGCTTTGAAAACCAAGCCAATGAACAGTCAGTGCCTGTGCCGGTGCCGGTGCCG GTGCCGGTGCCCGTGCCCAGCTTCAGCGGGTCGGAGGAACCTGTACGCAAGGAGAGCAAAACCCCACCCC CCTACAGTGTCTACGAGCGGACTCTGCGGCGCCCTGTCCCGCTACCTCACAGCCTCTCCATCCCTGTCAC CTCTGAGCCACCTGCTCTGCCCCCCAAGCCCTTGGCTGCGCGGTCCAGCCACCTGGAGAATGGGACCCGT AGGACTGAGCCTGGCCCTAGGCCCAGGCCCCTGCCCCGTAAGGTGTCTCAGCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172803
Insert Size	5937 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_172803.2, NP_766391.2
RefSeq Size	8072 bp
RefSeq ORF	5937 bp
Locus ID	238130
UniProt ID	P59764
Gene Summary	Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.