Scn10a (NM_009134) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Nav1.8; PN3; SNS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225081 representing NM_009134 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCTTTGGGTCCGTGGGAACTACCAACTTCAGACGGTTCACTCCAGAGTCGCTGGCAGAGA TCGAGAAGCAGATCGCTGCCCACCGCGCCGCCAAGAAGGGCAGACCTAAGCAAAGAGGACAGAAGGACAA GAGTGAGAAGCCCAGGCCTCAGTTGGACTTGAAGGCCTGTAACCAGCTGCCCAGGTTCTATGGCGAGCTC CCAGCAGAGCTGGTCGGGGAGCCCCTGGAGGACCTGGATCCTTTCTACAGCACACACCGGACATTCATAG TGTTGGATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCTCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCCGTCCACTCCTGGTTCTCCATATTTATCACTGTCACTATT TTGGTCAACTGTGTGTGCATGACCCGAACTGATCTTCCAGAGAAACTCGAGTATGCCTTCACTGTTGTTT ACACCTTCGAGGCTCTGATAAAGATACTGGCAAGAGGGTTTTGTCTAAATGAATTCACTTATCTTCGAGA TCCCTGGAACTGGCTGGACTTCAGTGTCATTACCCTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGGGCCCTGAAGACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACCATCCTCACAGTCTTCTGCCT GAGTGTCTTTGCCTTGGTGGGCCTGCAGCTCTTCAAGGGGAACCTCAAGAATAAATGCATTAAGAACGGC ACAGATCCGCACAAGGCTGACAATCTCTCATCTGAAATGGCAGGAGACATCTTCATCAAGCCCGGTACTA CGGATCCCTTGTTGTGTGGCAATGGATCTGATGCTGGCCACTGCCCTAATGATTATGTCTGCCGGAAAAC TTCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTCGCGTGGGCGTTCCTCTCACTGTTCCGT CTCATGACGCAGGACTCCTGGGAACGGCTGTACCAGCAGACACTCCGGGCTTCCGGGAAAATGTACATGG TCTTTTTTGTGCTGGTCATCTTCCTTGGATCATTCTACCTGGTCAATTTGATCTTGGCTGTGGTCACCAT GGCATATGAGGAACAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAGAAGAAGTTCAAGGAAGCC CTCGAGGTGCTGCAGAAAGAACAGGAGGTGCTGGCAGCGCTGGGCATTGACACAACCTCGCTCTATTCCC ACAACGGATCACCCTTAGCCCCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGATGTCAGA AGGCTCGACAGATGACAACAGATCACTACAATCCGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTTTCTTCTGGAAGACGCAGGGCTAGCCACAGCAGTGTGTTCCACTTCCGAGCACCCAGCCAAGACGTCT CATTTCCTGATGGGATCTTGGATGACGGGGTCTTTCATGGAGATCAGGAAAGCCGTCGAAGTTCCATATT GCTGGGCAGGGGTGCCGGGCAGGCAGGTCCTCTCCCCAGGAGTCCACTGCCTCAGTCCCCCAACCCTGGC CCTAGACGTGGAGAAGAGGGACAGCGTGGAGTGCCCACTGGTGAGCTTGCCACTGGAGCGCCTGAAGGCC CGGCACTCGATGCTGCAGGACAGAAGAACTTCCTGTCTGCAGACTACTTGAATGAACCTTTCCGAGCACA GAGGGCAATGAGTGTTGTCAGTATTATGACTTCTGTCATTGAGGAGCTGGAAGAGTCTAAGCTGAAGTGC CCACCCTGCTTGATCAGCTTAGCCCAGAAGTACCTGATATGGGAGTGCTGCCCCAAGTGGAAGAAATTCA AGATGGTGCTCTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTCACCATCACCCTGTGCATTGTGGTGAA TACCGTCTTCATGGCCATGGAACACTACCCCATGACTGATGCCTTCGATGCCATGCTCCAAGCCGGCAAC ATTGTCTTTACTGTGTTTTTTACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTACTACT TCCAGAAGAAGTGGAACATCTTCGACTGTGTCATCGTCACCGTGAGCCTGCTGGAGCTGAGCACATCCAA GAAGGGCAGCTTGTCTGTGCTCCGCACCTTCCGCTTGCTTCGGGTCTTCAAGCTGGCCAAGTCCTGGCCC ACCCTGAACATGCTCATCAAGATCATCGGGAACTCTGTGGGGGCCCTGGGCAACCTGACCTTCATCCTGG CCATCATCGTCTTTATCTTCGCCCTGGTGGGAAAGCAGCTCCTCTCAGAGAACTATGGGTGCCGCAGGGA TGGCATCTCCGTGTGGAATGGTGAGAGGCTGCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTCGTC GTCTTCCGGATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGGACTACA TCTGCCTCACCCTCTTCTTGACAGTGATGGTGCTAGGCAACCTGGTGGTGCTCAACCTATTCATCGCTTT ACTGCTGAACTCCTTCAGTGCGGACAACCTCACAGCCCCAGAGGATGACGGGGAGGTGAACAACTTGCAG GTAGCACTGGCCCGGATTCAGGTATTTGGCCATCGGGCCAGTCGGGCCATTACCAGTTACATCAGAAGCC ACTGCCGGCTCCGCTGGCCCAAGGTGGAGACCCAGCTGGGGATGAAACCCCCACTCACCAGCTGCAAAGC TGAGAACCACATTGCTACTGATGCTGTCAATGCTGCAGTGGGGAACCTGGCAAAGCCAGCTCTTGGTGGC CCCAAGGAGAACCACGGGGACTTCATCACTGATCCTAACGTGTGGGTCTCTGTGCCCATTGCTGAGGGGG AGTCCGACCTTGATGAGCTCGAGGAAGATGTGGAGCATGCTTCTCAGAGCTCCTGGCAGGAAGAGAGCCC CAAAGGGCAGGAGCTGCTGCAGCAAGTCCAAAAGTGTGAAGATCACCAGGCAGCCCGAAGCCCACCCTCC GGGATGTCCTCTGAGGACCTGGCTCCATACCTGGGGGAGAGATGGCAGAGGGAGGAGAGCCCTCGGGTCC CTGCCGAGGGAGTGGATGACACAAGCTCCTCCGAGGGCAGCACGGTGGACTGCCCGGACCCAGAGGAGAT CCTGAGGAAGATCCCTGAGCTGGCGGAGGAGCTGGACGAGCCCGATGACTGTTTCCCAGAAGGCTGCACT CGCCGCTGTCCCTGCTGCAAAGTGAACACCAGTAAGTTTCCTTGGGCCACGGGCTGGCAGGTGCGCAAAA CCTGTTACCGCATTGTGGAGCACAGCTGGTTTGAGAGTTTCATTATCTTCATGATCCTGCTCAGCAGTGG AGCGCTGGCCTTTGAGGATAACTACCTGGAAGAAAAGCCCCGAGTGAAGTCTGTGCTGGAGTACACTGAC AGAGTGTTCACCTTCATCTTTGTATTCGAGATGTTGCTCAAGTGGGTAGCTTATGGCTTCAAAAAATATT TCACCAATGCCTGGTGCTGGCTGGACTTCCTCATCGTGAATATCTCCCTCACAAGCCTCATAGCCAAGAT CCTCGAGTATTCAGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGGCCGCTGCGGGCT CTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCTTGGTGGGCGCCATTCCCTCCATCATGAACG TCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGTGTGAACCTCTTCGCCGGGAAATT TTCGAGATGTGTCGACACCAGAAGCAACCCATTTTCCGTCGTGAATTCGACATTCGTGACTAACAAGTCT GACTGTTACAATCAAAACAATACTGGCCACTTCTTCTGGGTTAACGTCAAAGTCAACTTCGACAACGTTG CTATGGGCTACCTCGCGCTTCTCCAGGTGGCAACCTTCAAAGGCTGGATGGACATTATGTATGCAGCTGT CGATTCTCGAGATATCAACAGTCAGCCCAATTGGGAGGAGAGCCTGTACATGTACCTATACTTCGTCGTC TTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTCGGGGTCATCATTGACAACTTCAATCAAC AGAAAAAAAAGCTAGGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCATGAA GAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTTTGAATAAGTACCAGGGCTTCGTGTTT GACATTGTGACCAGGCAAGCATTTGACATCATCATCATGGCTCTCATCTGCCTCAACATGATCACCATGA TGGTGGAGACCGACAATCAGAGCGAGGAGAAGACGAAGGTCCTGGGCAGAATCAACCAGTTCTTCGTGGC CGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTTCGGCAGTATTACTTCACCAACGGCTGGAAT GTGTTCGACTTCATTGTGGTGATTCTGTCCATTTCTAGTCTGTTGTTTTCTGCGATCCTTAGCTCACTAG AAAGTTACTTCTCCCCCACGCTCTTACGCGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCTGAT TCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAACATC GGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAATGTCATAG ATGAGGCTGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTTTTCCAGAT CACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGGACCCCCCTACTGCGACCCC AACCGGCCCAACAGCAATGGCTCCAAGGGGAATTGTGGAAGCCCAGCGGTGGGCATCCTCTTCTTCACCA CCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCAGTGATTCTGGAGAACTTCAATGT GGCCACAGAAGAGAGCACGGAGCCCCTGAGCGAGGACGACTTTGACATGTTCTATGAGACCTGGGAGAAG TTTGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTTGCAGACACACTCTCTGGCC CTCTTAGAATCCCAAAACCTAATCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGGAGATAA GATCCACTGTTTGGACATCCTCTTTGCCTTCACAAAGAATGTCTTGGGAGAATCTGGGGAGTTGGATTCT CTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGCATCCTATGAACCAATAGCAA CCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAGGCCTATCGGAACTACATGTT GCAACGCTCCTTGATGCTCTCCAACCCCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCACTCCCC AGGGAAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGACAAATCGGAAACTGCTTCTG CTACGTCTTTCCCACCATCCTATGACAGCGTCACCAGGGGCCTGAGTGACAGGGCCAACATTAGCACATC TAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAAGAGCCCTGGACCTCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_009134
Insert Size	5874 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009134.3, NP_033160.2
RefSeq Size	6413 bp
RefSeq ORF	5874 bp
Locus ID	20264
UniProt ID	Q6QIY3
Gene Summary	Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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