Myh13 (NM_001081250) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MyHC-eo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225068 representing NM_001081250 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCAGACGCAGAGATGGCCATATTTGGAGAAGCCGCTCCCTACCTCCGAAAACCAGAGAAGGAGA GGATCGAGGCTCAGAACCGCCCATTCGACTCGAAGAAAGCCTGCTTTGCTGTGGATGATAAAGAAATGTA CGTGAAAGGCATGATCCAAAGCAGGGAAAATGACAAAGTCATTGTCAAGACCCTAGACGACCGGGAGCTC ACTCTGAACAGTGACCAAGTCTTCCCCATGAACCCCCCTAAGTTTGACAAGATCGAGGACATGGCCATGA TGACCCACCTGCACGAGCCCGCTGTGCTGTACAATCTCAAAGAGCGTTATGCAGCCTGGATGATCTATAC CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG GCGGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTACC AGTTCATGCTAACAGATCGAGATAACCAATCCATCTTGATCACTGGAGAATCTGGGGCTGGAAAGACAGT GAACACGAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTCACCGGGGACAAGAAGAAAGAGCAGCAG CCGGGCAAAATGCAGGGAACGCTGGAGGATCAGATCATCCAAGCCAACCCCCTGCTGGAGGCCTTTGGAA ACGCCAAGACCGTGAGGAACGACAACTCCTCCAGATTTGGGAAGTTCATTCGGATCCACTTTGGAGCCAC AGGGAAGCTAGCATCAGCGGACATCGAAACCTATCTCCTGGAAAAATCCAGAGTGACATTCCAGTTATCC AGTGAAAGAAGCTATCACATTTTTTATCAAATCATGTCAAATAAGAAGCCAGAACTAATTGACTTGCTTC TGATTTCAACCAACCCCTTCGACTTCCCTTTCGTGAGCCAAGGAGAGGTCACAGTAGCTAGCATCGATGA CAGCGAGGAATTGTTGGCCACTGATAATGCCATTGACATCCTGGGCTTTAGCCCGGAGGAGAAAGTTGGG ATCTACAAGCTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAACAGAAGCAGAGAGAGGAGC AGGCTGAGCCAGATGGCACGGAAGTGGCTGACAAAGCTGGATACCTGATGGGACTGAATTCTGCAGAGAT GCTGAAAGGCCTGTGCTGTCCAAGAGTGAAGGTTGGAAATGAGTATGTCACCAAAGGACAGAATGTCCAG CAGGTGACCAACTCAGTGGGTGCCCTGGCCAAGGCTGTCTATGAGAAGATGTTCCTATGGATGGTCACCC GCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGGCTT TGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAGTTT TTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTG ACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATTGAGAAGCCAATGGGCATCTTCTCCATCCTGGA AGAGGAGTGCATGTTCCCTAAGGCCACAGACACCTCCTTCAAGAACAAGTTGTATGACCAGCACCTGGGA AAGTCCAACAACTTCCAGAAGCCTAAACCCACCAAAGGCAAGGCCGAAGCCCACTTCTCCCTGGTGCACT ATGCGGGCACTGTGGATTACAACATCGCTGGCTGGCTGGATAAGAACAAGGACCCACTGAATGAGACCGT GGTGGGGCTGTACCAGAAGTCCTCCCTGAAGTTGTTGTCCTTCCTCTTTTCCAACTACGCAGGAGCAGAA GCAGGTGACTCCGCAGGAGGCAAGAAGGGAGGAAAGAAGAAGGGCTCCTCCTTCCAGACTGTGTCTGCTG TGTTCAGGGAAAACTTAAACAAATTGATGACCAACCTAAGGAGCACCCATCCTCACTTTGTGCGATGCCT GATTCCCAATGAGACCAAGACTCCTGGTGTGATGGACCACTACTTGGTCATGCATCAGCTGCGCTGTAAT GGGGTCCTGGAGGGCATCCGGATCTGCAGGAAAGGGTTCCCCAGTCGAATCCTCTATGCTGACTTCAAAC AACGGTACCGGATCCTTAACGCCAGTGCCATCCCGGAAGGGCAGTTCATCGACAGCAAGAACGCCTCGGA GAAGCTCCTGAACTCCATCGATGTGGACCGAGAGCAGTTCCGATTCGGCCATACCAAGGTGTTTTTCAAA GCTGGGCTCCTGGGGCTTCTGGAAGAGATGAGAGACGAGAAGCTGGTGACCCTAATGACACGCACACAGG CAGTGTGCAGGGGCTACCTGATGAGGGTGGAGTTCAAGAAAATGATGGAGAGGAGGGAGTCCATCTTCTG CATCCAGTACAATGTTCGTTCCTTCATGAACGTCAAGCACTGGCCCTGGATGAACCTGTTCTTCAAGATC AAGCCCCTGCTGAAGAGCGCGGAGGCGGAGAAGGAGATGGCCACCATGAAGGAGGACTTCGAACGAGCCA AGGAAGACCTGGCTAGATCTGAGGCTCGTCGGAAGGAGCTGGAGGAGAAAATGGTCTCCCTGCTGCAGGA GAAAAATGACCTTCAGTTGCAAGTCCAGTCTGAAACAGAGAATCTAATGGATGCGGAGGAACGATGCGAG GGTCTCATCAAAAGCAAGATCCAACTGGAGGCAAAGGTCAAGGAACTGAATGAGAGGCTGGAGGAGGAGG AAGAGATGAATTCTGAGCTGGTTGCCAAGAAGAGGAATCTGGAAGACAAGTGTTCTTCTCTCAAAAGAGA CATTGACGACCTGGAGTTGACCCTGACTAAGGTCGAGAAGGAAAAGCACGCCACGGAGAACAAGGTGAAG AACCTCTCTGAAGAAATGACAGCGCTTGAGGAAACCATTTCCAAACTGACGAAAGAAAAGAAGTCTCTGC AGGAAGCCCATCAGCAGACCCTGGATGACCTGCAGGTTGAAGAAGATAAAGTCAACGGTCTCATCAAAAT CAATGTCAAGCTGGAACAGCAGACGGATGACCTCGAGGGCAGCTTGGAGCAGGAGAAGAAACTGCGGGCT GACCTGGAGAGAGTGAAGAGGAAGCTGGAAGGAGACTTGAAGATGTCCCAGGAATCCATCATGGATCTAG AGAATGACACGCAACAGTTGGAGGAGAAACTGAAGAAGAAGGAGTTTGAAATGAGTCAGTTACAAACCAG AATCGATGATGAGCAAGTCCTCAGTCTACAGCTGCAGAAGAAGATCAAAGAATTACAGGCCCGCACAGAA GAGCTAGAGGAAGAAATTGAAGCAGAGCACACAGTCAGAGCCAAAATTGAGAAGCAGCGCTCAGACCTAG CCAGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCAGTGGGGCTACTTCAGCCCAGATCGAGAT GAACAAGAAGAGAGAATCCGAGTTCCAGAAACTTCGAAGGGACCTGGAGGAGGCCACACTGCAGCACGAA GCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACACCGTGGCGGAGCTCGGGGAGCAGATCGACAACC TGCAGAGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGATGACATGGCCAG CAACATTGAAACAGTCTCCAAGTCAAAGAGTAACATGGAAAGAATGTGCCGGTCTGTGGAAGACCAATTT AACGAAATCAAAGCCAAGGATGACCAACAGACCCAGTTAATCCACGATCTGAACATGCAGAAGGCAAGGC TGCAGACCCAGAATGGCGAGCTGAGCCACCAAGTCGAAGAGAAGGAGTCTCTGGTTTCACAGCTCACCAA AAGCAAGCAGGCCCTCACCCAGCAGCTGGAGGAGCTTAAGAGGCAACTGGAGGAAGAAACCAAGGCCAAG AACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGGCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGAC AAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGCTGGCTCAGAGG CTCCAGGAGGCAGAGGAAAACACCGAGGCATCCAACTCCAAGTGTGCCTCCCTGGAGAAAACCAAGCAGA GGCTTCAGGGAGAAGTGGATGACCTGATGCTGGACTTGGAGAGGGCCAACACAGCCTGCGCCACCCTGGA CAAGAAACAAAGAAACTTCGACAAGGTCCTTGCAGAGTGGAAACAAAAACTGGATGAGAGCCAAGCTGAG CTGGAAGCTGCCCAGAAAGAGTCCAGGTCTCTCAGTACTGAAATCTTCAAGATGAGGAATGCCTATGAGG AGGTGGTGGATCAGTTAGAGACACTGAGACGGGAGAATAAAAACCTGCAAGAGGAGATTTCTGACTTAAC AGAGCAGATTGCAGAAACGGGCAAAAACCTCCAGGAAGTGGAAAAGACAAAGAAGCAAGTGGAGCAGGAG AAATCTGACCTCCAGGCTGCCTTGGAAGAGGTGGAGGGTTCCCTGGAACACGAGGAGAGCAAGATCTTGC GAGTCCAGCTAGAGTTAAGCCAGGTAAAATCTGAGCTTGATCGCAAGGTCACAGAAAAGGATGAGGAAAT CGAACAAATAAAAAGAAACAGCCAGCGTGCTGTGGAGGCCATGCAAAGTGTGTTAGATGCAGAAATCCGA AGCCGCAATGACGCCCTGAGACTGAAGAAGAAGATGGAGGGTGATCTCAACGAGATGGAGATTCAGCTGA GCCATGCCAACCGCCAGGTGGCAGAGACCCAGAAACACTTGCGCACGGTCCAAGGCCAGCTTAAGGATTC CCAGCTGCACCTGGACGATGCCCAGAGAAGTAACGAGGACCTCAAGGAGCAGCTAGCCATTGTGGAGCGC AGGAATGGCCTCCTGCAGGAGGAGCTGGAGGAAATGAAGGTGGCCCTGGAGCAGACAGAGCGGACCCGCA GGCTGTCAGAGCAGGAGCTGCTGGACTCCAGTGACCGTGTCCAGCTGCTCCATTCCCAGAACACGAGCCT GATAAATACCAAGAAAAAACTAGAGGCCGACTTGGCTCAATGCCAGGCAGAAGTGGAGAACTCCATCCAG GAGTCCAGAAACGCAGAAGAGAAGGCCAAGAAGGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGA AGAAGGAGCAGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCT GCAGCACCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAG GCCAGGGTGCGGGAGTTGGAAAGCGAGCTGGATGCTGAGCAGAAGAGAGGAGCTGAAGCTCTGAAGGGAG CCCACAAATATGAGCGCAAAGTTAAAGAGATGACTTACCAGGCCGAGGAGGACCGCAAGAACATCCTCCG GCTCCAGGACCTGGTGGACAAGCTGCAGGCCAAAGTGAAGTCCTACAAGAGGCAGGCAGAGGAGGCTGAG GAGCAAGCCAACACACAGCTATCCAGGTGCCGGAGGGTCCAGCACGAGCTAGAGGAGGCTGAGGAGAGGG CAGACATCGCTGAGTCTCAAGTCAACAAGCTAAGGGCCAAGAGCCGTGATGTGGGAGGCCAGAAGATGGA AGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081250
Insert Size	5817 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081250.1, NP_001074719.1
RefSeq Size	5817 bp
RefSeq ORF	5817 bp
Locus ID	544791

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