Myh6 (NM_010856) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A830009F23Rik; AA517445; alpha-MHC; Myhc-a; Myhca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225064 representing NM_010856 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACGCCCAGATGGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCC TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT TAAGGCCAAGGTCGTGTCCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAGACGGTGACC ATAAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCACGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTATACCTA CTCAGGCCTCTTCTGCGTCACCGTCAACCCCTATAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCC GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCGGGGAAGACTGTGAA CACAAAACGTGTCATCCAGTACTTTGCCAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGAAAATCCT AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCCGCTCTGGAGGCCTTCGGCAACG CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAATTCATCAGGATCCACTTTGGAGCTACTGG AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCTGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT GAGAGGAACTACCACATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG TCACCAACAACCCATACGACTACGCCTTCGTCTCTCAGGGAGAGGTGTCCGTGGCCTCCATTGATGACTC TGAGGAGCTCTTGGCCACTGATAGTGCCTTTGATGTGCTGAGCTTCACGGCAGAGGAGAAGGCTGGTGTC TACAAGCTGACAGGGGCCATCATGCACTACGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CGGAGCCTGATGGCACAGAAGATGCTGACAAATCTGCCTACCTTATGGGGCTGAACTCAGCTGACCTGCT CAAGGGCCTGTGTCACCCTCGGGTGAAGGTGGGGAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAA GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA TCAACGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGA GATCTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGGAGTTTATCGACT TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTCGAGGA GGAGTGCATGTTCCCCAAGGCCTCAGACATGACCTTCAAGGCCAAGCTGTATGACAACCACCTGGGCAAA TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCCTTGGTCCACTATG CTGGCACCGTGGACTACAACATTATGGGCTGGCTGGAAAAGAACAAGGACCCACTCAATGAGACGGTGGT GGGTTTGTACCAGAAGTCCTCCCTCAAGCTCATGGCTACACTCTTCTCTACCTATGCTTCTGCTGATACC GGTGACAGTGGTAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAAACAGTGTCTGCTCTCCACC GGGAAAATCTGAACAAGCTGATGACAAACCTGAAGACCACCCACCCTCACTTTGTGCGCTGCATCATTCC CAACGAGCGAAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTG CTGGAGGGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGT ATCGCATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACT GCTGGGCTCCCTGGACATTGACCACAACCAATACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGC CTGCTGGGGCTGCTCGAGGAGATGCGAGATGAGAGGCTGAGCCGTATCATCACCAGAATCCAGGCCCAGG CCCGAGGGCAGCTCATGCGCATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTTATCCA GTGGAACATTCGGGCCTTCATGGGGGTCAAGAATTGGCCATGGATGAAGCTCTACTTCAAGATCAAACCG CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCAACATGAAGGAGGAGTTTGGGCGAGTCAAAGATG CACTGGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA TGACCTACAGCTCCAAGTGCAGGCGGAACAAGACAACCTCAATGATGCAGAGGAGCGCTGTGACCAGCTG ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA TGAACGCCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGAAGGATATTGA TGACCTGGAGCTGACGCTGGCCAAGGTGGAAAAGGAAAAGCATGCAACAGAGAACAAGGTTAAAAACCTA ACAGAGGAGATGGCTGGGCTGGATGAAATCATTGCCAAGCTGACCAAAGAGAAGAAAGCTCTGCAAGAAG CCCACCAGCAAGCCCTCGATGACCTGCAGGCTGAAGAAGACAAGGTCAACACGCTGACCAAGTCCAAAGT CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAAGTGCGCATGGACCTA GAGCGAGCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAATG ACAAGCTTCAGCTGGAAGAAAAGCTCAAGAAGAAAGAGTTCGACATCAGTCAGCAGAACAGTAAAATTGA GGACGAGCAGGCCCTGGCTCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG GAGGAGGAGCTGGAGGCAGAGCGCACAGCCCGGGCTAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGG AGCTGGAGGAGATCAGTGAGAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAATAA GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACG GCGGCGGCCCTGCGCAAGAAGCATGCTGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGC GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACAT GGAGCAGATCATCAAGGCCAAGGCCAACCTGGAGAAAGTGTCCCGGACACTGGAGGACCAGGCCAATGAG TACCGCGTGAAGCTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA CAGAGAACGGGGAGTTGGCTAGGCAACTGGAAGAAAAGGAGGCATTGATTTCCCAGCTGACCCGAGGCAA GCTCTCCTACACCCAGCAGATGGAGGACCTCAAGAGGCAACTGGAGGAGGAAGGCAAGGCCAAGAACGCC CTGGCCCACGCACTGCAATCATCCCGGCATGACTGTGACCTGCTGAGGGAACAGTATGAAGAAGAAATGG AGGCCAAGGCTGAGCTACAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA TGAGACGGATGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAG GATGCAGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGTTCCTCCCTGGAGAAGACCAAGCACAGGCTGC AGAATGAGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAA GCAGAGGAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAG TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTC TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA GCTGGGAGAAGGGGGGAAAAACGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG GAGCTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGTGCCC AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCA GGCCAAGCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGC AATGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCAGG CCAATAGAATAGCCTCAGAGGCACAGAAACACCTGAAGAATTCTCAAGCTCACTTGAAGGACACCCAGCT CCAGCTGGATGATGCTGTCCATGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAACGGCGCAAC AACCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGG CAGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAA CCAGAAGAAGAAGATGGAGTCAGACCTGACCCAACTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGT AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCAATGATGGCTGAGGAGCTGAAGAAGG AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCA CCGTCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCAGAGTCGGTGAAGGGCATGAGGA AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTAATGCGGCTGCA GGACCTGGTGGACAAGCTACAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAG GCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACA TCGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGAAGATGCACGACGA GGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010856
Insert Size	5817 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010856.4, NP_034986.1
RefSeq Size	6015 bp
RefSeq ORF	5817 bp
Locus ID	17888
UniProt ID	Q02566
Gene Summary	Muscle contraction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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