Myh7 (NM_080728) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B-MHC; beta-MHC; Myhc-b; MyHC-I; Myhcb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225051 representing NM_080728 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGCAGAGATGGCTGCATTTGGGGCTGCAGCCCCCTTCCTGCGGAAGTCTGAGAAGGAGAGGC TGGAGGCACAGACCAGGCCCTTTGACCTCAAGAAAGATGTTTTTGTGCCCGATGACAAAGAAGAGTTTGT CAAGGCCAAGATCGTGTCCCGAGAGGGTGGCAAAGTCACTGCTGAGACGGAGAATGGCAAGACGGTGACT GTGAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCATGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCTTCCTGGATGATCTATACCTA CTCGGGCCTCTTCTGCGTCACCGTCAACCCCTACAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCTGGGAAGACTGTCAA CACTAAGAGGGTCATCCAATATTTTGCTGTTATTGCCGCCATTGGGGACCGCAGCAAGAAGGACCAGACC CCAGGCAAGGGTACCCTGGAAGATCAAATCATCCAAGCCAACCCTGCTCTGGAGGCCTTTGGCAATGCCA AGACAGTTCGGAATGACAACTCCTCTCGATTTGGGAAATTCATCCGAATCCATTTTGGGGCAACAGGAAA GTTGGCATCTGCAGACATAGAGACCTACCTTCTGGAAAAATCCAGGGTTATTTTCCAGCTGAAAGCAGAA AGAGATTATCACATTTTCTACCAAATCCTGTCTAATAAAAAGCCTGAGCTTCTAGACATGCTGCTGATCA CCAACAACCCCTACGATTATGCGTTCATCTCCCAAGGAGAGACGACTGTGGCCTCCATTGATGACTCTGA AGAGCTCATGGCCACGGATAGCGCCTTTGACGTGCTGGGCTTCACTCCAGAAGAGAAGAACTCCATTTAC AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGG AACCAGACGGCACTGAAGAGGCTGACAAATCAGCCTACCTCATGGGGCTGAACTCAGCCGACCTGCTTAA GGGGCTGTGCCATCCTAGAGTCAAAGTGGGCAACGAGTACGTCACCAAGGGGCAGAATGTCCAGCAGGTG TCATACGCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCA ATGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGAGAT CTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAAC CACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGACCTTCATAGACTTCG GCATGGACTTGCAGGCCTGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTTGAGGAGGA GTGCATGTTCCCCAAGGCCACAGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAGTCC AACAACTTCCAGAAGCCTCGAAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCTTTGGTCCACTATGCTG GCACTGTGGACTACAATATCCTGGGCTGGCTACAGAAGAACAAGGACCCACTCAATGAGACGGTGGTGGG TTTGTACCAGAAGTCTTCCCTCAAGCTGCTCAGCAATCTATTTGCCAACTATGCTGGAGCTGATGCCCCG GCGGACAAAGGCAAAGGCAAGGCAAAGAAAGGCTCATCCTTTCAGACCGTGTCTGCTCTGCACAGAGAAA ATCTGAACAAACTTATGACAAACTTGCGCTCCACGCACCCTCACTTTGTACGCTGCATCATCCCCAATGA GACAAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTGCTGGAG GGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGTATCGCA TCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACTGCTGGG CTCCCTGGACATTGACCACAACCAATACAAGTTTGGTCACACCAAGGTGTTCTTCAAGGCGGGCCTGCTG GGGCTGCTGGAGGAGATGCGTGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCCCAGTCCCGAG GTGTGCTCTCCAGAATGGAGTTCAAGAAGCTGCTGGAGCGCAGAGACTCCCTGCTGATTATCCAGTGGAA CATTAGGGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG AAGAGCGCGGAGACGGAGAAGGAGATGGCCACCATGAAGGAGGAGTTTGGGCGAGTCAAAGATGCACTAG AGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT GCAGCTCCAAGTGCAGGCGGAACAAGACAACTTGGCGGATGCAGAGGAGCGCTGTGACCAGCTGATCAAG AACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGATGAATG CCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGCGGGATATCGATGACCT GGAGCTGACGCTGGCCAAGGTGGAGAAGGAAAAGCATGCAACAGAGAACAAGGTGAAAAACCTGACAGAG GAGATGGCTGGTTTGGATGAGATCATTGTCAAGCTGACAAAGGAGAAGAAAGCTCTGCAAGAGGCCCACC AGCAGGCTCTGGATGACCTGCAGGCTGAGGAAGACAAGGTCAATACTCTGACCAAGGCCAAGGTCAAGCT GGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAGGTGCGCATGGACCTAGAGCGA GCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACGCAGGAGAGCATCATGGACCTGGAGAATGACAAGC AGCAGTTGGATGAGCGACTCAAAAAGAAGGACTTTGAGTTAAATGCACTCAATGCCAGGATTGAGGATGA GCAAGCCCTGGGCAGTCAGCTGCAGAAGAAGCTCAAGGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG GAGCTGGAGGCCGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGGAGCTGG AGGAGATCAGTGAAAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAACAAGAAGCG CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACGGCGGCG GCCCTGCGCAAGAAGCATGCCGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGCGGGTGA AGCAGAAGCTGGAGAAAGAGAAAAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCA GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCTTGGAAGACCAGATGAATGAGCACCGG AGCAAGGCCGAGGAGACGCAGCGTTCTGTCAATGACCTCACCAGCCAGCGGGCCAAGCTGCAGACAGAGA ATGGGGAGCTGTCCCGGCAGCTGGACGAGAAGGAGGCTCTGATCTCTCAGCTAACCCGAGGCAAGCTCAC ATATACACAGCAGCTGGAGGACCTCAAGAGGCAACTGGAGGAGGAGGTCAAGGCCAAGAACGCGCTGGCC CACGCACTGCAGTCAGCACGGCATGATTGTGACCTGCTGAGGGAACAGTATGAGGAGGAGACAGAGGCCA AGGCTGAGCTACAGCGAGTCCTGTCCAAGGCCAATTCAGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC GGATGCCATACAGAGGACAGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAGGATGCA GAGGAGGCAGTGGAGGCTGTCAATGCCAAGTGTTCCTCTCTGGAGAAGACCAAGCACAGGCTGCAGAATG AGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAAGCAGAG GAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAGTCTTCC CAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTCTGGAGC ACCTAGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAGCAGCTGGG CTCCACGGGGAAGAGCATCCATGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCCGAGAAGCTGGAGCTG CAGTCGGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGCGCCCAGCTAG AGTTCAACCAGATCAAGGCAGAGATTGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCAGGCCAA GCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGCAATGAG GCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCATGCCAACC GTATGGCTGCTGAGGCCCAGAAACAAGTGAAGAGCCTCCAGAGTCTGCTGAAGGACACTCAAATCCAGCT GGATGATGCTGTCCGTGCCAATGACGACCTGAAAGAGAACATCGCCATCGTGGAACGGCGCAACAACCTG CTGCAGGCGGAGCTGGAGGAGCTTCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGGCAGAGC AGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAACCAGAA GAAGAAGATGGATGCAGACCTATCCCAGCTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGTAGGAAC GCAGAGGAGAAGGCCAAGAAGGCTATCACAGATGCCGCCATGATGGCTGAGGAGCTGAAGAAGGAGCAGG ACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCACCGTCT GGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGGGTCCGG GAGCTGGAGAATGAGCTGGAGGCTGAGCAAAAGCGCAATGCAGAGTCAGTGAAGGGCATGAGGAAGAGTG AGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAGGAAGAACCTACTGCGGCTGCAGGACCT GGTGGACAAGCTGCAGCTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAGGCCAAC ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACATCGCCG AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGGGCCTGAATGAGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_080728
Insert Size	5808 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080728.2, NP_542766.1
RefSeq Size	6054 bp
RefSeq ORF	5808 bp
Locus ID	140781
UniProt ID	Q91Z83
Gene Summary	Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.[UniProtKB/Swiss-Prot Function]

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