Ank3 (NM_170729) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900054D09Rik; AI314020; An; Ank; Ank-3; AnkG; Anky; Ankyrin-3; Ankyrin-G
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225043 representing NM_170729 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAGCCAAAGGAGAAGCCCGCCAAGCCTGCTCATAGGAAGAGGAAAGGAAAAAAGTCTGATG CCAACGCAAGTTACTTAAGAGCAGCTCGGGCAGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG AGTGGACGTCAACATCTGTAACCAGAATGGATTGAATGCACTCCATCTTGCTTCCAAAGAAGGCCATGTG GAAGTGGTCTCTGAGCTGCTGCAGAGGGAAGCCAATGTTGATGCCGCCACAAAGAAAGGAAACACGGCCT TACACATCGCATCTTTGGCTGGGCAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAATGTCAA CGCACAATCTCAGAATGGCTTCACACCATTGTATATGGCAGCCCAGGAGAACCACCTGGAAGTCGTCAGG TTTCTTCTGGACAATGGCGCCAGCCAAAGCCTGGCCACAGAGGACGGCTTCACGCCATTGGCCGTGGCTC TGCAACAAGGTCATGACCAAGTCGTGTCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC AGCCCTCCACATCGCAGCCCGGAAAGACGACACCAAGGCAGCAGCTCTGCTCCTGCAGAATGACACAAAC GCGGACGTGGAGTCAAAGAGTGGCTTCACCCCGCTCCACATAGCTGCCCACTATGGGAACATCAATGTGG CCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGACTTCACCGCACGGAATGACATCACTCCCTTACACGT TGCCTCGAAGCGAGGAAATGCAAATATGGTGAAGCTATTGCTGGACCGGGGTGCGAAGATCGATGCCAAG ACCAGGGACGGTCTGACTCCGTTGCACTGTGGGGCGAGAAGTGGCCATGAGCAGGTGGTAGAGATGTTGC TTGACAGATCCGCCCCCATCCTTTCAAAAACCAAGAATGGATTGTCGCCACTGCACATGGCCACACAAGG AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGACGACGTCACCAACGACTAC CTGACTGCCCTCCATGTGGCTGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG CTAGCCCCAATGCCAAAGCCCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGCATCCG AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC CATGTTGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA CCACCAATGTGAGAGGAGAGACGGCATTGCATATGGCGGCTCGGTCCGGACAAGCAGAAGTGGTGCGGTA TCTGGTCCAAGATGGGGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACTCCACTCCACATCTCAGCC CGACTTGGGAAAGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCATCCCCCAATGCAGCAACAACTT CTGGGTACACCCCCCTTCACCTTGCGGCCAGAGAGGGGCATGAGGATGTAGCTGCGTTCCTCCTGGATCA TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACCCCTCTGCACGTGGCAGCCAAATACGGAAAG CTTGAAGTCGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCCGATGCCGCAGGGAAGAGCGGGCTAACTC CACTGCATGTAGCAGCGCATTACGATAATCAGAAAGTGGCCCTTCTGCTCTTGGACCAGGGAGCCTCACC CCACGCAGCCGCAAAGAATGGCTATACACCACTGCACATCGCGGCCAAGAAGAACCAGATGGACATAGCC ACGTCCCTGCTGGAGTACGGTGCTGATGCAAACGCGGTTACCCGGCAAGGGATTGCGTCCGTCCATCTTG CGGCACAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTGAGTAGAAACGCGAATGTCAACCTGAGCAA TAAGAGCGGTCTCACCCCACTCCACCTGGCTGCTCAAGAAGACCGAGTGAATGTGGCCGAGGTCCTTGTC AACCAGGGGGCCCATGTGGATGCTCAGACAAAGATGGGCTACACCCCGCTCCATGTGGGCTGTCACTATG GAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATA CACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCC TCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGG TGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT GAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGGTGAAGATGCCATCACAGGG GACACTGACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTT ACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTT GAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCAC CTGACGTTCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATA ATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGG CTCCATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACC CGCATCACGTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGAT TAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCC TCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAACTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGG AAGGAACATCAGTTTGACAGTAAAAACGAAGACCTCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCG ACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTGCAGAATTATCACAAAGGATTTCCCCCAGTATTTTGC CGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACC GTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCGCCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTC AGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCTTGGGAACAAAGCAACATTTAGCCCAATTGTCACGGT AGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATCACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAA GGCGTGTCCAATGGGTACAAGGGGGATGCCACGCCCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCA CCTCACCAGCTCAATGGGAAGACATCACAGGAACAACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTT CACAACCAACGTTTCAGCCAGATTCTGGCTGGCGGACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCC TCCCAGCTGTACAGAGAGCTGATATGCGTTCCCTACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACG ACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTATGACAGACGACAGGGTGGACAAAACCCTGGAGCAGCA GGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGACATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGAT TGCTATGGAAACCTGGCCCCTCTGACCAAAGGAGGACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAG AAAACAGACTGCCATTTTCCATCAAGATCAGAGACACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCT GAAGGAGCCAAAGACAACAAAGGGATTACCCCAAACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCA CATAAAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCT ACTTGACTGAACCCAGCATGAGTCCGCAGAGTCCTTGTGAGCGGACGGATATCAGGATGGCGATAGTAGC CGATCACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAACCAA ATACGTGTGGAAAATCCCAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAG ACGGAAAGAATGCCACAACTGATGCCTTAACTTCGGTCTTAACGAAGATTAACCGGATAGACATTGTAAC TCTGCTGGAAGGACCAATATTTGATTATGGGAATATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAAT GTTTTCCATGACCCAGTTGATGGTCACCCTTCCTTTCAAGTGGAGCTGGAGACCCCCATGGGGTTGTACT GCACACCACCCAACCCTTTCCAGCAAGATGACCATTTTAGTGATATCTCTAGCATAGAGTCTCCCTTTAG GACCCCCAGTAGACTGAGTGACGGGCTGGTGCCTTCCCAGGGAAACATAGAGCATCCAACAGGTGGACCT CCAGTGGTAACCGCAGAGGACACTTCTTTAGAAGACAGCAAAATGGACGATTCTGTAACTGTAACAGACC CGGCCGACCCACTGGACGTAGATGAGAGCCAGTTGAAGGACCTGTGTCAGAGCGAGTGTGCTCAGTGCTG GGCGAGTGTGCCCGGGATCCCAAACGACGGTCGGCAGGCAGAGCCACTGAGACCGCAGACTAGAAAAGTA GGCATGAGCTCTGAACAGCAGGAAAAAGGAAAATCTGGTCCTGATGAGGAAGTGACAGAAGACAAGGTCA AATCTCTGTTTGAGGACATTCAACTTGAAGAAGTAGAGGCTGAGGAGATGACAGAAGACCAGGGGCAGGC TATGCTTAACCGTGTTCAGCGAGCAGAACTGGCAATGTCTTCACTTGCAGGTTGGCAGAACGAGACGCCA AGTGGAAGCCTAGAGTCCCCAGCGCAAGCTCGAAGACTAACTGGTGGGTTACTGGACCGTCTGGATGACA GCTCTGACCAGGCTCGGGATTCTATTACCTCATACCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGG AAACCACACAGCGGAAGTCATTCCAGAAGCAAAGGCAAAACCCTACTTCCCGGAATCCCAAAACGATATA GGGAAACAGAGCATCAAGGAGAACCTGAAACCAAAAACACACGGATGTGGTCGCACTGAGGAACCAGTGT CGCCCCTCACAGCCTACCAGAAATCTCTGGAAGAAACCAGCAAGCTTGTCATAGAAGACGCACCTAAACC CTGTGTGCCTGTCGGCATGAAAAAGATGACCAGGACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAG GAAGAAGAGGGGTCCACCAGGTCAGAGCCTAAGCAGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAA TCCGGAACGTGGAGAAGAAAACCCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_170729
Insert Size	5769 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_170729.2, NP_733925.2
RefSeq Size	9908 bp
RefSeq ORF	5769 bp
Locus ID	11735
UniProt ID	G5E8K5
Gene Summary	This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (8) has multiple differences in the coding region but maintains the reading frame, compared to variant 2. This results in a shorter isoform (h), compared to isoform b.

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