Chd4 (NM_145979) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9530019N15Rik; AA617397; BC005710; D6Ertd380e; Mi-2beta; mKIAA4075
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225032 representing NM_145979 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGCTCAGCGGGCAGTGAGGAGGAGGATATGGACGCACTTC TGAACAACAGCCTGCCCCCACCCCATCCAGAAAATGAGGAAGACCCAGACGAGGATTTATCCGAAGCAGA GACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAAGAAACCTCGAGACCCTAAAATCCCTAAGAGCAAGCGC CAAAAAAAGGAGCTGGGGGACAGCTCTGGGGAGGGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCTC TGCGCTCAGACAGTGAGGGCAGCGACTATACTCCTGGCAAGAAGAAGAAAAAGAAGCTCGGACCTAAGAA AGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGAGGAGGAAGAGGAGGACGAGGATGATGACTCGAAGGAA CCTAAGTCATCAGCCCAGCTTTTAGAAGACTGGGGTATGGAAGACATCGACCATGTGTTCTCAGAGGAGG ATTATCGGACCCTCACCAACTACAAGGCCTTCAGCCAATTTGTTAGACCCCTGATTGCTGCTAAAAACCC CAAGATTGCTGTCTCCAAGATGATGATGGTCTTGGGTGCAAAGTGGAGGGAATTCAGTACCAACAACCCC TTCAAGGGCAGCTCCGGAGCATCAGTGGCAGCTGCGGCGGCGGCGGCTGTGGCTGTGGTTGAGAGCATGG TGACAGCTACTGAAGTCGCCCCTCCCCCTCCCCCTGTGGAGGTGCCGATCCGCAAGGCCAAGACCAAGGA GGGCAAAGGTCCCAATGCTCGGAGGAAGCCGAAAGGCAGCCCGCGAGTGCCTGACGCCAAGAAGCCCAAA CCCAAGAAAGTAGCTCCTCTGAAGATCAAGCTGGGAGGGTTCGGCTCCAAGCGGAAGAGATCCTCGAGTG AGGACGACGATTTAGATGTAGAGTCTGACTTCGACGATGCCAGTATCAACAGCTATTCTGTTTCGGATGG TTCCACCAGCCGCAGTAGTCGGAGCCGTAAGAAACTCCGGACCGCTAAAAAGAAAAAGAAAGGCGAGGAG GAGGTGACTGCTGTGGATGGTTATGAGACAGACCACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGCG AGATCATCCTGTGTGATACCTGTCCCCGAGCCTACCATATGGTGTGCCTGGACCCAGACATGGAGAAGGC CCCGGAGGGCAAGTGGAGCTGTCCGCACTGTGAGAAGGAGGGGATCCAGTGGGAAGCTAAGGAGGACAAT TCTGAGGGTGAGGAGATTCTGGAAGAAGTCGGGGGGGACCCAGAAGAGGAGGATGACCATCACATGGAAT TCTGTCGCGTCTGCAAGGACGGCGGGGAGCTCCTGTGCTGTGACACATGCCCTTCTTCCTACCACATCCA CTGCCTGAACCCCCCGCTGCCAGAGATCCCAAACGGCGAATGGCTCTGTCCCCGCTGTACTTGTCCAGCT CTTAAGGGCAAAGTGCAGAAGATCCTAATTTGGAAGTGGGGCCAGCCACCGTCTCCCACACCAGTGCCTC GGCCTCCAGATGCTGACCCTAATACCCCGTCGCCCAAGCCCTTGGAGGGGCGGCCAGAGAGGCAGTTCTT TGTGAAATGGCAAGGCATGTCTTACTGGCACTGCTCCTGGGTGTCAGAACTGCAGTTGGAGCTGCACTGT CAGGTGATGTTCAGAAACTACCAGCGGAAGAATGATATGGACGAGCCGCCTTCTGGGGACTTTGGTGGAG ATGAAGAGAAGAGCCGGAAGCGAAAGAACAAGGACCCTAAGTTTGCAGAGATGGAAGAGCGCTTCTACCG CTATGGAATAAAACCGGAGTGGATGATGATCCACCGCATCCTCAACCACAGTGTGGACAAGAAGGGCCAC GTTCACTATTTAATCAAGTGGCGAGACCTGCCCTATGACCAGGCGTCCTGGGAGAGCGAGGACGTGGAGA TTCAGGACTACGACCTGTTCAAGCAGAGCTACTGGAACCACAGGGAGTTAATGAGGGGTGAAGAAGGACG ACCCGGCAAGAAGCTTAAGAAGGTGAAGCTACGGAAACTGGAAAGGCCTCCGGAGACCCCAACTGTTGAT CCCACCGTGAAGTATGAGCGACAGCCGGAGTATCTGGATGCGACGGGTGGAACCCTGCATCCCTACCAAA TGGAAGGCTTAAACTGGCTCCGCTTCTCCTGGGCTCAGGGAACCGATACTATCTTGGCTGATGAGATGGG CCTTGGGAAAACTGTGCAGACAGCAGTCTTCCTCTACTCCCTCTATAAGGAGGGTCACTCCAAAGGCCCC TTCTTAGTGAGCGCTCCTCTGTCCACCATCATCAACTGGGAGAGAGAGTTTGAGATGTGGGCTCCTGATA TGTATGTGGTAACCTATGTGGGTGACAAGGACAGCCGTGCCATCATCCGGGAAAATGAGTTCTCCTTTGA AGACAATGCCATTCGTGGTGGCAAGAAGGCCTCGAGGATGAAGAAAGAAGCATCGGTGAAATTCCATGTT CTGCTGACATCCTATGAGTTGATCACCATTGATATGGCCATCTTGGGTTCTATTGACTGGGCCTGCCTCA TTGTGGATGAAGCCCATCGGCTGAAGAACAACCAGTCTAAGTTCTTTCGAGTTTTGAATGGTTACTCACT CCAGCACAAGCTGTTGCTAACTGGAACTCCGTTACAGAACAATCTAGAGGAACTGTTTCATCTGCTCAAC TTTCTCACCCCTGAGAGGTTCCACAACTTAGAAGGCTTCTTGGAGGAGTTTGCAGACATTGCCAAAGAGG ACCAGATTAAAAAACTCCACGACATGCTGGGGCCTCATATGTTGCGGCGGCTCAAAGCTGACGTCTTCAA GAATATGCCATCCAAGACAGAGCTGATTGTGCGCGTGGAGTTGAGTCCTATGCAGAAGAAGTACTACAAG TATATTCTCACGCGGAATTTCGAAGCACTTAATGCTCGAGGTGGTGGCAACCAGGTTTCTCTGCTGAACG TGGTGATGGATCTTAAGAAATGCTGCAACCACCCTTATCTCTTCCCTGTGGCAGCAATGGAAGCCCCTAA GATGCCTAATGGTATGTATGATGGCAGTGCCCTAATCAGAGCATCTGGGAAGTTGTTGCTGCTGCAGAAG ATGCTTAAGAACCTGAAAGAGGGAGGGCATCGTGTGCTCATCTTCTCCCAGATGACCAAGATGTTGGACT TGCTAGAGGATTTTTTAGAACATGAAGGTTATAAATATGAACGTATTGATGGTGGAATCACTGGGAACAT GCGTCAGGAGGCCATTGATCGCTTCAATGCACCGGGTGCTCAACAGTTCTGCTTCTTGCTTTCCACTCGA GCTGGGGGCCTTGGGATCAATCTGGCCACTGCGGACACAGTTATTATATATGACTCTGACTGGAACCCCC ATAATGACATCCAGGCCTTTAGCAGAGCCCACCGTATTGGGCAAAATAAGAAGGTGATGATCTACCGGTT TGTGACCCGTGCATCAGTGGAGGAGCGCATCACGCAGGTGGCAAAGAAGAAGATGATGCTGACACATTTA GTGGTACGGCCTGGCCTGGGCTCCAAGACAGGCTCAATGTCTAAACAGGAGCTTGATGATATCCTCAAAT TTGGCACTGAGGAGCTATTCAAGGATGAAGCCACGGATGGAGGAGGAGACAACAAGGAGGGAGAAGACAG CAGTGTTATCCACTATGACGATAAGGCCATTGAACGACTGCTGGATCGAAACCAGGATGAGACTGAAGAC ACAGAATTGCAGGGCATGAATGAATATTTGAGCTCATTCAAAGTGGCTCAGTATGTGGTACGGGAAGAAG AGATGGGGGAGGAAGAGGAGGTAGAACGGGAAATCATAAAACAGGAAGAAAGTGTGGATCCTGACTACTG GGAGAAATTGCTGCGGCACCATTATGAGCAGCAGCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAA AGAATCCGTAAACAGGTCAACTACAATGATGGCTCACAGGAGGACCGAGATTGGCAGGACGACCAGTCCG ACAACCAGTCCGATTACTCAGTGGCCTCAGAGGAAGGTGATGAAGACTTTGACGAACGGTCAGAAGCTCC CCGCAGGCCCAGTCGCAAGGGCCTGCGGAATGATAAAGATAAGCCATTACCTCCTCTGTTGGCCCGTGTT GGTGGGAATATTGAAGTACTTGGTTTTAATGCTCGTCAGCGAAAAGCATTTCTTAATGCAATTATGCGAT ATGGCATGCCACCTCAGGATGCTTTTACCACTCAGTGGCTTGTGAGAGATCTTCGAGGCAAGTCAGAGAA AGAATTTAAGGCTTATGTGTCACTCTTCATGCGACATTTGTGTGAGCCTGGGGCAGATGGGGCTGAGACC TTTGCTGATGGCGTCCCACGAGAAGGCCTGTCTCGTCAACATGTTCTCACTAGGATCGGTGTCATGTCTC TGATTCGAAAGAAGGTTCAGGAGTTTGAACATGTTAATGGACGCTGGAGCATGCCCGAACTGGCTGAAGT AGAGGAAAACAAAAAAATGTCTCAGCCAGGTTCACCTTCTCCAAAGACTCCTACACCTTCCACACCAGGG GACACACAACCCAATACTCCTGCGCCTGTGCCACCTGCTGAGGATGGGATAAAAATAGAGGAAAATAGCC TTAAAGAAGAAGAGAGCACAGAAGGAGAGAAGGAAGTTAAATCTACAGCCCCTGAGGCGACTGTGGAGTG TGCACAGCCCCCTGCCCCTGCCCCTGCCACTGCCCCTGCCACTGCCACTGCCCCTGAGGATGACAAGGCT CCTGCTGAGCCTCCTGAGGGGGAGGAAAAAGTGGAAAAGGCAGAGGTGAAGGAGAGAACAGAGGAGCCTA TGGAGACAGAGGCCAAAGGTACTACTGAGGTGGAGAAAGTGGAGGAGAAGTCTGCGGTAGACCTGACCCC GATCGTGGTGGAAGACAAAGAAGAGAAGAAGGAGGAGGAGGAGAAAAAGGACGTGATGCTTCAGAATGGA GAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAAACAGCAAACAGCGTTTCATGTTCAACATTG CAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGAAGAGCGGGCAGCCACAGTCACTAAGAA GACTTACGAGATCTGGCACCGTCGCCATGATTACTGGCTGCTGGCTGGCATTATAAACCACGGCTATGCC CGGTGGCAGGACATCCAGAATGACCCACGGTATGCCATCCTCAATGAGCCTTTCAAGGGTGAAATGAATC GTGGCAATTTTCTAGAGATCAAGAATAAATTTTTAGCTCGAAGATTCAAGCTCTTAGAACAAGCCCTGGT GATCGAGGAGCAGCTTCGCAGGGCAGCTTACCTGAACATGTCCGAGGACCCCTCTCACCCGTCTATGGCC CTTAACACCCGCTTCGCTGAGGTGGAGTGTTTGGCAGAAAGTCACCAGCACCTGTCCAAGGAGTCGATGG CAGGGAATAAGCCGGCCAATGCAGTCCTACACAAAGTCCTGAAACAGCTGGAGGAACTGTTGAGTGACAT GAAAGCTGATGTGACGCGGCTCCCAGCCACCATTGCCCGCATCCCCCCAGTCGCTGTGAGGTTACAGATG TCAGAGCGGAACATTCTCAGCCGCCTGGCAAACAGGGCGCCAGAACCTCCCCCACAGCAGGTAGCCCAGC AGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_145979
Insert Size	5748 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_145979.2, NP_666091.1
RefSeq Size	6438 bp
RefSeq ORF	5748 bp
Locus ID	107932
UniProt ID	Q6PDQ2
Gene Summary	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.[UniProtKB/Swiss-Prot Function]

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