Ticrr (NM_029835) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730590G19Rik; BC028450; Lrrk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225013 representing NM_029835 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCTGTCACAAAGTGATGCTGCTGGTGGACACAGCGGGCGTCTCTGCTCCTCATAGCCCGGCCC GACGCGCCGCCCTCCGGCTCCTCACTTACCTGAGCTGCCGCTTCGGCCTTGCCAGGGTCCACTGGACCTT CAAGTTCTTCGACTCTCAGGGGGCACGGAGCCGGCCCTCCCGCGTGTCCGACTTCCGCGAGCTGGGGAGC CGCTCCTGGGAGGACTTCGAGGAGGAGCTGGAGGCCAGGCTGGGGGATCGCCCTCCCGGAGCTCACCTGC CGGGCCCGACGCCCAGAGCCACCCACACGCACGGTGCCCTGATGGAGACGCTGCTCGACTACCAGTGGGA CCGGCCGGAGATCACGTCGCCCACCAAGCCGATCCTGCGGAGTAGCGGCAGGAGGCTGCTGGACGCCGAC GGCGAGGCCAGGGAGGCCCAGGCCGCGCTCGGGGGCTTTGGGAACGCCGTCTTCCTCCTGGCGCCCTGTC CTCACTCGCAGAGGGAGCTGCTTCAGTTCGTGTCCGGGTGCGAGGCACAGGCTCAACGCGTGCCGCTCAC CCCCAAGCAGGTGATGGAGAAGGTGTTGCCCAAGAGAGTCCAAGAGGTTATGATCGCCCGAAATATCACC TTGTACTGGGTGGACACCACCGAGCGATCTAAGTTGTGGGCTTCCCCAGACCATGTTGGATACTGGACAG TGTGTGAGCTGCTACACCATGGAGGAGGTACCATCTTGCCAGCTGAAACTTGGAGCCTGGGATTTACTAA AGCCAGGGAAACAGTGCTGCCATGTGGTGGCGAGCTGTCACACAAACCTCACCCCTCCCCGTGGATCTCA GCTCTGCCAATAGATGCCACTGTAAACTGTTTGCTGTATAATTCTGAGTATGAAGCCTCATTTCCACGAA TTGAAGGAACATTATTTCTCCCTGTTCAAGGCAAAGAAATTGAAGAAACATGGGCAATCTCCCTGGAGCC TTTAGCCATGCATCAGAGACATTTTCAAAAGCCAGTCAGAATTGTCCTAAGAGGCTCAGTGGCACAGTGG TCTCTCCCAGTGAGTAGTGCTTTAGGCACTGACAGTTGGATGCTACAAAGTCCAGAGGAACACAGGTCAA CTCAAAGGCTGTTGTTTCAGGAGCTGGTAAGCAGGCTGACTGCTGAAGAATTCCATCTGGTTGCCAGTGT GGATCCTGGTGAAGGCTGGCCCCCCATAACGGGAATTATTTCCCCATTCTCTGCCAATGCCATGATTCTC ACAGTGTTCCGAGCCAAAGAAGCTGAATTTCAAAGCCATTTTCTCCAAACAGCTGCGACTGAAGGTTCCC AGGATACAGCTTCCCTTTTCTCAGATGTTGTAGATAGTGTCCTGAATCAGAGTCATAATTTATTTGAAGA CCCTGCGTCTTCTGCCCCCTGTGTTCCAGAGTGGGTCCAGCAGGAGCTTAGTCATACCAGCTCCTGGAGT CCAGCTCTTGTCGAAAAGTGGTTTCCTTTCTCCAACGCCAGTGGTGCTACTTCAGACTTGATGGAATCAT TTTGGTTGTTACACGCTGCCTCACCTGATAATGATGAATCCTCCAAAACCGAAAGTGAACTAACACGCTG CCTCTCTGAGCTCTACCAGAGATCTCACGAAGAATCTACTGTAGTCAATCAAGAACGCAGCAGAAAGAAA CGTGGTATCCCTCGAACTCCAGTGAGACAGAAGATGAATACCATGTCCCGCTCCTTGAAGATGTTGAATG TGGCAAGGCTAAATGTAAAGGCTCAGAAATTACATCCAGATGGTAGTCCAGACACGGCTGTAGAGAAAGG GCTCCAGAAAGCTGTCATTGGCAGAACAGCAGACAAATTGGAAGATAGAGGAAGGATATTAAGAAGTTCT AAACTTAAAGAATTTAAAACCGAGGAGGAGCTTCTGGCTTACATACATGACAACTACCAAAAGGCTGTGG CTACGGAAGAAATCACATTGTATTCATGTGCACAAAATATGGTCTCAACCATTAAAATGTTCCTAAAATC AAAGGACATCAAGGAATTAGAAGTGGCCTGCCTGAGTCATGTGAACAGTAACCTCTTAAAAACCAGCAAG ACCCTTAGACAGAACCTAGCCGGAAAGATGGATACAGAGGACAAAGTCGGAGAGTGCCAGCTTCAGGTAT TTCTTCGTCTGGAGATGTGTGAGCAGTGCCCCTCAGTCCTGGACCGCCCAGATGAGGTGGAGCGCATAGT GGAGGAGGTGACAGATTTGCTGCGTCTGGTGTGTCTCACTAAGGACTCAGCGTACCTGTCAGAGTTTCTG GAGGAGATCTTGAGATTGTATATTGGCTCCATCCCCGGGACAATTGGACAACTCTACCACAGCCTGGGGT TAAAAATTCCTCAGAAGCTGGCTGGAGTCCTTCCTACAGGGTTTTTCAGTGATGATTCTATGTCTCAAGA GAGCATGTCACCACCTCCCTCATCAAGTACTCACAGATCAGTGTCGGCCATTACTGAGTCTGAGCAGCTG GAGGAGCTTCGGACCAGATCAGCCAAGAAGAGAAGGAAAAATGCACTAATAAGACATAAAAGTATTGCAG AGATTTCCCAGACTCTTCGACAAATTGAAGTTCCCAAAGTGTCCAAGAGAGCTACAAGAAATGAGAACTC CCACTCTGCTTCCATACAGCTGCCAGTCCCGAGGAAAGACACAATACAGGAAGTAACAAAAGTTCGAAGA AACCTCTTCAACCAGGAAATGCTTTCTCCTTCAAAGAGGGGGCTAAAGAAGGGGCTGCCTCGCAGCCATT CTGTATCTGCTTTGGAATGTCTCCACCATAAACAGGACAAGTTCAAGAAGACCAAAAGCAGCACATTTCA GGGTTATTGCAAACTGCTGACTAAGAGTGTGGCTGAGACCCCAGTCCACAAGCAAATCTCCAGGAGGCTG CTCCATAGACAAATCAAGGGCAGGTCTTCTGATCCTGGTCCTGATATTCATGTAGTTGAAGAGTCTCCTG AAAAAGAGGATGAAATGACTTTGAGAAGAAGTCCTCGAATCAAGCAGCTGTCATTTAGCAGAACGAACTC TGGCTCCTTCTATTCTGTATCTCAGCCGAAATCTCGAAGTGTGCAAAGAATCCACTCGTCCCAGCAGGAG TCAGAACAAAGAGAAAATTTTCCAGTTCAAAGTATTCAGTCTCCTAAAACACTTCTTTTTGGGGCATTGT CTGAGATTCCCAGCTCCTCTAAGAAGGGTTCAGCCCAAATTAAGAGATCCTTAAGAAGCATGTTGGATTC TGAGATATCTACATCTTACGAGACTCCCAAAAAGAGTAACCAGAAATCTCCAAGCTTTTCTAAAACTACA CCAAGAAGATTCCCTCGTACAGCACAGACCTTACTATACACTCCAGAAAGACTACAAAACTCCCCTACAG AAATGACATCAGCTGAAGGGACCATTTCTGAAGCAACCATAAAAACCCCCTCCTCACACGGTTACAACTC ACCATTTGCATCAAAAGTCACTTCTCAAAAGACAGTCTCCCCAGCAAAAGAAGAGACCTCTCCTCCCCTC ACAAAGCTACCCAGCACACCCAGAGAGTCTGATGTCCAGCCACCTCAATGTTCGTCAGACTGCACATGGC CACACTCAGTGAATTCCAGTCCAGAAGGTCCTTATTACCCAGCGTCCCCACCTCCTATGGCTGGCCAGGC CAGGAGTCAGTGTCTGACCCCTATAAGATACTCCTTCAGAACCCCTCCACGTACAGCCCTTGCTGGCACG TCTAAGCAGCAGGAGCACCAAGAGCTCCCTCTCCCCAGGGCCTCTCAGACACAAGAACCTCCACAGGGAC TGGAGAAGAAAGCTCTCAAAATCCCAAAGAAACCAGCACACACAAGTACTTCACCTCTTTCTCCAGAGGA ACATTATTCTGGATGTGATGTCTCCCCACATCAACCTAGGAACTCTTTGTCAGCCTCCCCTCCTCCTGGA GAACTGAATTGGAAAGAACACCAGACATCACCCAGTGTAACTTCATCTGTCTCTTGTCCTGTTCCTTCAA CTCCTCCTAGAACCCCACAGAGAATGACCTGCCCCATACCACCTTCTCCACCATCCAAGCTTCGGAGATC ATGTAGAAAGAAGTCCTGTCCTCCTCAGGACTTCCCAGAGTGCCATCCTGGACCCTCTGCTGCTCCCGTG CTTAGCTCAGCCACCAGCCCAGGAGCTGTCACAGGCTCTAGAGAGGAGCAGTCTCAGTTTTCTGAAGGAC AGAGTTACCTAGGCACTGGTTTCAGAAGTGACTGTCATGTGTCCTCTCCTGTGCTCACTGCAAGTGACAC AAAGTGCTTGCCACTGATTGATGAAGCCCAACTCCATGGGCTTAAAAACCAAGAGGTAAAAAGTGGCATC TTGCCAGGGGAAGAAGGTGAGGAACCAGAGAGCACTATTGCCGACGAGCTTCCCTCTGTGTCTGACCCTG GGATTCTTGTACCTGCGCCTTCCTCTGTGTCTAGCTCCTCTGAGCTGCTGCCCTACCCCCTGTGCTGTAC AGCAGACGGAAAGCAGAGGCAAGATGCTGCTCAGCAGGGGAGTCCACGAGCTTCCGAGGCCACCAGCAGC CCTCAGACCTACGAAGTTGAGCTGGAGATGCAAGCTTCTGGCCTTCCCAAGCTCCGGATTAAAAAAATAG ACCCTGGAGTTCTTTTGGAAGCTGAGGCCCTAGGAAAGGAAGCCCCCCTGGGAGAGGAGGGTGCCCTCCC TGCTCTCTGTATGCCCAAGGCCAGCAAGTCCTCCGGGAGAACTGAGCACCCTTACTTGTCACCCCCTTGT CTCCGCCCCTCACACAGCACACCTGGCAAGAATGGAGGTCAAACCTTCATCTGCCAATCTTGTACTCCCT CTCGCTGTCCCCCCAGTACCCCCTCTCCATTTCAAGCAGATGCTGGGGTTTCTTGGACACCATCCCCTAA GCAAAGTGGGAAGACCACTCCTGAAATTATCAAAGACTGGCCCCGCAGGAAGAGGGCAGTGGATTGCAGT GCTGGACCCTCTGCTGGGAGAGGCGAGGCCAGTATGGACCTTCCTGGGTCCTTGTCACTGCTTGAGCCTG AGCCTGAGGGAAAGGAGCGAAGCCTTGAACAAGACCTCTCTAAGGTTCTCATCTCAGAGGAGTTTGAACT AGAGGGGGTGTGTCAGTTGCCAGACCAGTCACCTCCCAAAGACAGTGCCTCTGTGACAGAGGAAACCTCC TGGGGACAGTTTGGATTAGGCAGAAAGAGATTCCTGTCCGCCAAGGAAGAATCTGAATACAAAGTCAAAA GGGTCTGTGATTCCCTGAGTGAGGATCCCCAAGCTAGCAAGCAGAAAGAGTGCTCTCCACGCTGGAGTGC ACTACCACTCCACTCAGTAGGGGACGACGAGGTGTTTGTTTCTGGCTCCACCCCACCCTCTGGTTGCATG GTTCGAAGCTGTCTCTCTGCCAGTGGTCTGCAGGCCTTGACCCAGTCTCCACTGCTGTTCCAAGGAAGAA CGCCCTCCTCTCATAGCACGGACACCAGAGATGAGGAAGTGGATGTGTTCCCCTCTACTGCTGAAGAGTC TCCGTTCAGCCACACCCTCTCCCGGAAACGGCCTTTCAGAACCTACACGAGAAAGAAGCTTATTAGCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_029835
Insert Size	5670 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029835.1, NP_084111.1
RefSeq Size	7213 bp
RefSeq ORF	5670 bp
Locus ID	77011
UniProt ID	Q8BQ33
Gene Summary	Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating in CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation (By similarity).[UniProtKB/Swiss-Prot Function]

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