Ubr3 (NM_001081548) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110059H15Rik; 4833421P10Rik; A130030D10Rik; AA409735; AA414972; AA422631; AI646861
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225012 representing NM_001081548 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCCGCGGCGGCCGTCGGGGACCCGCAGCCGCCCCAGCCGGAGGCGCCGGCGCAGG GGCTGGCTTTAGACAAAGCGGCCACCGCCGCTCATCTGAAGGCCGCTCTCAGCCGGCCGGATAACCGTGC GGGCGCCGAGGAGCTGCAGGCGCTGCTGGAGAGGGTGCTGAACGCCGAGCGGCCGCTGGCCGGGGCTGCG GGCGGCGAGGAGGCGGCGGGCGGCGGCGGCGGCGGCCCGGGCGAGGCGGAGGAGGATGCCCTGGAGTGGT GTAAGTGCCTTCTGGCCGGCGGCGGCGGCTACGAGGAGTTCTGCGCGGCCGTGCGGGCCTACGACCCCGC GGCGCTCTGCGGCCTGGTCTGGACGGCCAACTTCGTGGCCTACCGCTGCCGGACGTGCGGCATCTCCCCC TGCATGTCGCTGTGCGCGGAGTGCTTCCACCAGGGCGACCACACTGGACACGACTTCAACATGTTCCGCA GCCAGGCCGGGGGCGCCTGCGACTGCGGGGACAGCAACGTAATGCGGGAGAGCGGGTTTTGTAGAAGACA TCAGATTAAATCAAGTTCAAATATTCCCTGTGTCCCCAAAGACTTACTGATGATGTCTGAATTTGTTCTT CCAAGATTCATTTTTTGTCTTATTCAGTACTTAAGAGAAGGCTATAATGAACCAGCTGATGCACCATCAG AAAAAGATCTGAACAAAGTCCTTCAGCTTTTGGAGCCTCAGATTTCCTTTTTAGAAGACCTGACTAAAAT GGGAGGAGCCATGAGGTCTGTTCTTACTCAGGTGTTAACCAACCAGCAGAACTACAAAGACCTGACTGCG GGTCTTGGAGAAAATGCTTGTGCAAAGAAAAGTCACGAGAAGTACCTTATAGCTTTGAAGAGCTCCGGAC TGACATATCCCGAGGATAAGCTGGTGTATGGGGTCCAGGAACCAGCTGCTGGTACTAGCACTCTGGCAGC TCAAGGTTTTGCAGGTGCTACTGGAACACTGGGGCAGATAGACTCTTCAGATGAGGAGGACCAGGATGGG AGTCAAGGCTTGGGCAAGAGAAAGAGGGTGAAGCTAAGCAGTGGCACCAAAGATCAATCCATAATGGATG TTTTGAAACATAAAAGTTTTTTAGAAGAACTATTATTTTGGACAATAAAGTATGAGTTCCCACAGAAGAT GGTAACTTTCTTGCTCAATATGCTCCCAGATCAAGAGTATAAGGTTGCTTTTACAAAAACATTTGTTCAG CATTATGCTTTTATTATGAAAACACTGAAGAAAAGTCACGAATCAGATACAATGTCCAACAGAATTGTGC ATATTAGTGTTCAGTTGTTCAGCAATGAGGAGCTAGCCAGACAGGTGACAGAAGAGTGTCAGCTCCTGGA CATCATGGTCACTGTGTTGTTGTACATGATGGAGAGCTGCCTCATCAAGAGTGAGCTGCAAGATGAAGAG AACAGTTTACATGTAGTGGTGAACTGTGGAGAAGCCTTACTGAAGAATAACACCTACTGGCCCCTTGTCA GTGATTTTATTAATATTCTTTCTCATCAAAGTGTGGCTCAGAGGTTTCTGGAGGATCACGGCCTGTTAGT TACATGGATGAACTTTGTGTCTTTCTTTCAAGGTATGAACTTGAACAAGCGAGAGCTCAATGAGCACGTG GAATTTGAGTCTCAGACCTACTATGCTGCCTTTGCTGCTGAGCTGGAAGCCTGTGCACAACCCATGTGGG GGCTTTTATCACATTGTAAAGTTAGGGAAACTCAAGAGTATACCAGGAATGTTGTAAGGTACTGCCTTGA AGCTCTTCAAGATTGGTTTGATGCTATTAACTTTGTAGACGAGCCAGCTCCTAACCAAGTCACTTTCCAC CTACCACTGCACCGCTACTATGCTATGTTTTTGAGTAAGGCTGTGAAATGTCAAGAACTAGATTTGGATT CACTTTTACCGGATCAGGAAATGTTAATGAAACTAATGATTCACCCACTCCAAATTCAAGCTAGTCTTGC TGAAATTCACAGTAACATGTGGGTAAGAAATGGTCTGCAAATCAAAGGACAAGCCATGACCTATGTCCAG TCTCATTTCTGTAACTCCATGATTGACCCAGACATTTACTTACTACAGGTCTGTGCTTCTAGACTTGACC CAGACTACTTTATATCATCTGTCTTTGAAAGATTTAAGGTGGTGGACTTACTGACCATGGCTTCACAGCA CCACAACATGGTGCTCGATGTGGAGCACGAGCGGTCGATGCTAGAGGGTGCTCTCACGTTCCTCGTGATT CTTTTGAGTCTTCGTTTACACCTAGGGATGTCTGACGATGACATTCTCAGGGCAGAGATGGTGGCCCAGC TGTGTATGAACGACAGGACACATAGCTCTTTGCTTGACCTCATCCCAGAAAATCCCAACCCCAAAAGTGG CATTATTCCTGGAAGTTATAGCTTTGAATCAGTTTTATCTGCTGTAGCGGACTTTAGGGCTCCCATTTTT GAACCTGGAGGCTCTATGCAACAAGGCATGTACACTCCCAAAGCTGAAGTCTGGGATCAGGAGTTTGATC CTGTCATGGTCATTCTTCGAACCGTTTACCGCCGAGACGTGCAGTCTGCAATGGACAGATACACAGCATT TTTAAAACAGAGTGGAAAATTCCCTGGCAATCCCTGGCCTCCGTATAAGAAAAGAACATCACTCCATCCC AGCTATAAAGGTCTTATGAGACTTCTGCACTGTAAAACTTTACACATCGTGCTCTTCACTCTGCTTTACA AGATTCTGATGGATCATCAAAATCTGTCTGAGCATGTGCTGTGCATGGTCTTGTACCTGATTGAGTTAGG CCTGGAGAACTCTGCTGACGATGACTCCGAGGAAGAGGTATCAATGGGTGGACCAGAGCGTTGTCATGAC AGTTGGTTCCCTGGCAGTAACTTGGTATCAAACATGAGGCACTTTATAAATTATGTTAGAGTGCGAGTCC CAGAGACGGCTCCTGAGTTGAAAAGGGATCCCCTCGCCAGCACGAGCTCTGACGCTTTGGACTCTTTACA AAATTCTGGTACAGCTCAAGTTTTCAGCTTAGTAGCAGAGCGTAGAAAGAAATTTCAGGAGATCATCAAT CGCAGCAACAGTGAAGCAAATCAGGTGGTTCGGCCCAAGATTCCAAGTAAATGGTCAGCTCCTGGTTCCT CTCCCCAGTTAACCACAGCCATTTTGGAAATTAAGGAAAGCATATTGTCTTTGCTAATAAAACTTCACCA CAAACTCTCAGGAAAACAAAACTCTTACTATCCTCCATGGCTTGATGACATAGAAGTTTTAATACAACCA GAAATTCCCAAATACAATCATGGAGATGGTATAACTGCTGTAGAAAGGATTTTGCTAAAAGCTGCAGTGC AAAGCAGAATGAACAAGCGCATCATTGAGGAGATATGCAGAAAAGTGACCCCTCCTGTGCCACCCAAAAA AATCACCGCAGCGGAGAAGAAAACTCTGGACAAGGAGGAAAGGCGACAAAAGGCTAGAGAAAGGCAGCAG AAATTGCTCGCAGAGTTTGCCTCACGACAGAAAAGCTTTATGGAAACTGCAATGGATGTTGATTCTCCTG AGAACGATATTCCTATGGAAATTACCACAGCAGAACCACAAGTTTCTGAGGCCGTGTATGACTGTGTTAT TTGTGGACAGAGTGGTCCTTCTTCTGAAGACAGACCTACAGGACTGGTTGTACTGTTACAAGCATCATCA GTTTTAGGCCAGTGCCGGGACAATGCTGAGCCAAAAAAGTTGCCGATCGCTGAAGAGGAGCAGATTTACC CTTGGGACACATGTGCAGCAGTTCATGATGTGAGGCTTTCATTACTGCAGCGTTATTTTAAAGATAGTTC TTGCCTTTTGGCAGTATCAATTGGCTGGGAAGGAGGTGTTTATGTACAAACTTGTGGCCACACGTTGCAC ATAGATTGTCATAAATCCTATATGGAGTCGTTACGGAATGACCAGGTTCTTCAGGGCTTCTCAGTGGACA AAGGAGAATTCACGTGTCCCCTCTGTAGACAGTTTGCCAACAGTGTTCTTCCATGTTATCCTGGAAGCAA TGTGGAAAATAACCTTTGGCAACGCCCTTGTAACAAAAGCACGCAAGATCTCATAAAGGAAGTGGAAGAG CTGCAGGGACGGCCGGGAGCTTTCCCAGTAAGCATCAGTTCAGAAACCAACTTAAGTAAAGAAATGGAAT CTGTAATGAAAGATATAAAAAATACCACTCAGAAGAAATACAGAGACTATAGCAAGACCCCAGGCTCACC AGACAACGAGTTTCTCTTTATGTACTCTGTTGCTAGAACCAATCTGGAGCTGGAACTGATTCATAGAGGA GGCAGTCTCTGCTCCGGTGGTCCAAGCACAGCGGGGAAAAGATCTTGTCTGAATCAGCTGTTCCATGTAT TAGCCTTGCACATGCGGCTTTATACCATTGATTCTGAATATAATCCATGGAAAAAACTCACCCAGTTAGT GGAAGATATGAATTCACAGGTGGGGAATGAAGACCAGCAGCCAGAAGTTCCAATTCTTTATCATGATGTG ACATCCCTTCTGCTCATCCAGATCTTAATGATGCCACAACCCTTACGTAAAGAGCACTTCACCTGCATTG TGAAAGTACTCTTCACCCTGCTGTACACACAGGCCCTGGCGGCGCTCTCAGTTAAGGGCACTGAAGAGGA CCGGTCAGCCTGGAAACACGCCGGGGCTCTTAGAAAGGATACATGTGATGCAGAAAAGTGTTACGAAGTG CTGTTGAGCTTTGTTATAAGTGAGCTGTCTAAAGGGAAGTTATATTATGAAGAAGGCGCTCAGGAGTGTG CGATGGTCAGTCCTATTGCTTGGTCTCCTGAGTCCATGGAAAGATACATACAGGATTTCTGCTTGCCTTT CCTCAGGGTCAGCAGCCTTCTTCAGCACCACCTCTTTGGGGAAGACTTACCCAGTTGCCAGGAAGAAGAA GAATTTTCAGTCCTTGCCAGCTGCCTGGGACTTCTGCCAACTTTTTACCAAACAGATCACCCGTTCATCA GTGCCTCGTGTCTGGACTGGCCAGTTGCAGCGTTTGACATTATAACTCAGTGGTGTTTTGAGATAACATC ATTTACGGAGAGACACGCAGAGCAAGGAAAGGCCTTGCTTATCCAAGAATCAAGATGGAAGTTACCACAC CTGTTACAGTTGCCTGAGAATTACAACACTATTTTTCAGTACTATCATAGAAAAACCTGCAGTGTCTGCA CCAAGGTTCCTAAAGACCCAGCTGTTTGCCTCGTCTGCGGCACTTTCGTATGCCTGAAAGGCCTGTGCTG CAAGCAGCAAAGTTACTGTGAATGTGTGCTGCATTCTCAGAATTGTGGTGCAGGAACAGGAATTTTCCTT TTAATCAACGCGTCAGTGATCATCATCATCCGAGGCCACCGCTTTTGCCTCTGGGGTTCCGTCTACTTGG ATGCTCATGGAGAAGAGGACCGGGACCTCAGGCGAGGCAAGCCTCTCTACATCTGTGAGGAGCGGTACAG AGTTCTCGAGCAGCAGTGGGTTTCCCACACGTTTGACCACATCAATAAAAGATGGGGTCCGCACTACAAT GGGCTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001081548
Insert Size	5679 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081548.2, NP_001075017.2
RefSeq Size	8081 bp
RefSeq ORF	5679 bp
Locus ID	68795
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (PubMed:17462990). Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (PubMed:17462990). May play a role in Shh signaling by mediating the ubiquitination of Kif7 (PubMed:27195754). May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A (PubMed:27331610).[UniProtKB/Swiss-Prot Function]

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