Ubr3 (NM_177783) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110059H15Rik; 4833421P10Rik; A130030D10Rik; AA409735; AA414972; AA422631; AI646861
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225011 representing NM_177783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCCGCGGCGGCCGTCGGGGACCCGCAGCCGCCCCAGCCGGAGGCGCCGGCGCAGG GGCTGGCTTTAGACAAAGCGGCCACCGCCGCTCATCTGAAGGCCGCTCTCAGCCGGCCGGATAACCGTGC GGGCGCCGAGGAGCTGCAGGCGCTGCTGGAGAGGGTGCTGAACGCCGAGCGGCCGCTGGCCGGGGCTGCG GGCGGCGAGGAGGCGGCGGGCGGCGGCGGCGGCGGCCCGGGCGAGGCGGAGGAGGATGCCCTGGAGTGGT GTAAGTGCCTTCTGGCCGGCGGCGGCGGCTACGAGGAGTTCTGCGCGGCCGTGCGGGCCTACGACCCCGC GGCGCTCTGCGGCCTGGTCTGGACGGCCAACTTCGTGGCCTACCGCTGCCGGACGTGCGGCATCTCCCCC TGCATGTCGCTGTGCGCGGAGTGCTTCCACCAGGGCGACCACACTGGACACGACTTCAACATGTTCCGCA GCCAGGCCGGGGGCGCCTGCGACTGCGGGGACAGCAACGTAATGCGGGAGAGCGGGTTTTGTAGAAGACA TCAGATTAAATCAAGTTCAAATATTCCCTGTGTCCCCAAAGACTTACTGATGATGTCTGAATTTGTTCTT CCAAGATTCATTTTTTGTCTTATTCAGTACTTAAGAGAAGGCTATAATGAACCAGCAGCTGATGCACCAT CAGAAAAAGATCTGAACAAAGTCCTTCAGCTTTTGGAGCCTCAGATTTCCTTTTTAGAAGACCTGACTAA AATGGGAGGAGCCATGAGGTCTGTTCTTACTCAGGTGTTAACCAACCAGCAGAACTACAAAGACCTGACT GCGGGTCTTGGAGAAAATGCTTGTGCAAAGAAAAGTCACGAGAAGTACCTTATAGCTTTGAAGAGCTCCG GACTGACATATCCCGAGGATAAGCTGGTGTATGGGGTCCAGGAACCAGCTGCTGGTACTAGCACTCTGGC AGCTCAAGGTTTTGCAGGTGCTACTGGAACACTGGGGCAGATAGACTCTTCAGATGAGGAGGACCAGGAT GGGAGTCAAGGCTTGGGCAAGAGAAAGAGGGTGAAGCTAAGCAGTGGCACCAAAGATCAATCCATAATGG ATGTTTTGAAACATAAAAGTTTTTTAGAAGAACTATTATTTTGGACAATAAAGTATGAGTTCCCACAGAA GATGGTAACTTTCTTGCTCAATATGCTCCCAGATCAAGAGTATAAGGTTGCTTTTACAAAAACATTTGTT CAGCATTATGCTTTTATTATGAAAACACTGAAGAAAAGTCACGAATCAGATACAATGTCCAACAGAATTG TGCATATTAGTGTTCAGTTGTTCAGCAATGAGGAGCTAGCCAGACAGGTGACAGAAGAGTGTCAGCTCCT GGACATCATGGTCACTGTGTTGTTGTACATGATGGAGAGCTGCCTCATCAAGAGTGAGCTGCAAGATGAA GAGAACAGTTTACATGTAGTGGTGAACTGTGGAGAAGCCTTACTGAAGAATAACACCTACTGGCCCCTTG TCAGTGATTTTATTAATATTCTTTCTCATCAAAGTGTGGCTCAGAGGTTTCTGGAGGATCACGGCCTGTT AGTTACATGGATGAACTTTGTGTCTTTCTTTCAAGGTATGAACTTGAACAAGCGAGAGCTCAATGAGCAC GTGGAATTTGAGTCTCAGACCTACTATGCTGCCTTTGCTGCTGAGCTGGAAGCCTGTGCACAACCCATGT GGGGGCTTTTATCACATTGTAAAGTTAGGGAAACTCAAGAGTATACCAGGAATGTTGTAAGGTACTGCCT TGAAGCTCTTCAAGATTGGTTTGATGCTATTAACTTTGTAGACGAGCCAGCTCCTAACCAAGTCACTTTC CACCTACCACTGCACCGCTACTATGCTATGTTTTTGAGTAAGGCTGTGAAATGTCAAGAACTAGATTTGG ATTCACTTTTACCGGATCAGGAAATGTTAATGAAACTAATGATTCACCCACTCCAAATTCAAGCTAGTCT TGCTGAAATTCACAGTAACATGTGGGTAAGAAATGGTCTGCAAATCAAAGGACAAGCCATGACCTATGTC CAGTCTCATTTCTGTAACTCCATGATTGACCCAGACATTTACTTACTACAGGTCTGTGCTTCTAGACTTG ACCCAGACTACTTTATATCATCTGTCTTTGAAAGATTTAAGGTGGTGGACTTACTGACCATGGCTTCACA GCACCACAACATGGTGCTCGATGTGGAGCACGAGCGGTCGATGCTAGAGGGTGCTCTCACGTTCCTCGTG ATTCTTTTGAGTCTTCGTTTACACCTAGGGATGTCTGACGATGACATTCTCAGGGCAGAGATGGTGGCCC AGCTGTGTATGAACGACAGGACACATAGCTCTTTGCTTGACCTCATCCCAGAAAATCCCAACCCCAAAAG TGGCATTATTCCTGGAAGTTATAGCTTTGAATCAGTTTTATCTGCTGTAGCGGACTTTAGGGCTCCCATT TTTGAACCTGGAGGCTCTATGCAACAAGGCATGTACACTCCCAAAGCTGAAGTCTGGGATCAGGAGTTTG ATCCTGTCATGGTCATTCTTCGAACCGTTTACCGCCGAGACGTGCAGTCTGCAATGGACAGATACACAGC ATTTTTAAAACAGAGTGGAAAATTCCCTGGCAATCCCTGGCCTCCGTATAAGAAAAGAACATCACTCCAT CCCAGCTATAAAGGTCTTATGAGACTTCTGCACTGTAAAACTTTACACATCGTGCTCTTCACTCTGCTTT ACAAGATTCTGATGGATCATCAAAATCTGTCTGAGCATGTGCTGTGCATGGTCTTGTACCTGATTGAGTT AGGCCTGGAGAACTCTGCTGACGATGACTCCGAGGAAGAGGTATCAATGGGTGGACCAGAGCGTTGTCAT GACAGTTGGTTCCCTGGCAGTAACTTGGTATCAAACATGAGGCACTTTATAAATTATGTTAGAGTGCGAG TCCCAGAGACGGCTCCTGAGTTGAAAAGGGATCCCCTCGCCAGCACGAGCTCTGACGCTTTGGACTCTTT ACAAAATTCTGGTACAGCTCAAGTTTTCAGCTTAGTAGCAGAGCGTAGAAAGAAATTTCAGGAGATCATC AATCGCAGCAACAGTGAAGCAAATCAGGTGGTTCGGCCCAAGATTCCAAGTAAATGGTCAGCTCCTGGTT CCTCTCCCCAGTTAACCACAGCCATTTTGGAAATTAAGGAAAGCATATTGTCTTTGCTAATAAAACTTCA CCACAAACTCTCAGGAAAACAAAACTCTTACTATCCTCCATGGCTTGATGACATAGAAGTTTTAATACAA CCAGAAATTCCCAAATACAATCATGGAGATGGTATAACTGCTGTAGAAAGGATTTTGCTAAAAGCTGCAG TGCAAAGCAGAATGAACAAGCGCATCATTGAGGAGATATGCAGAAAAGTGACCCCTCCTGTGCCACCCAA AAAAATCACCGCAGCGGAGAAGAAAACTCTGGACAAGGAGGAAAGGCGACAAAAGGCTAGAGAAAGGCAG CAGAAATTGCTCGCAGAGTTTGCCTCACGACAGAAAAGCTTTATGGAAACTGCAATGGATGTTGATTCTC CTGAGAACGATATTCCTATGGAAATTACCACAGCAGAACCACAAGTTTCTGAGGCCGTGTATGACTGTGT TATTTGTGGACAGAGTGGTCCTTCTTCTGAAGACAGACCTACAGGACTGGTTGTACTGTTACAAGCATCA TCAGTTTTAGGCCAGTGCCGGGACAATGCTGAGCCAAAAAAGTTGCCGATCGCTGAAGAGGAGCAGATTT ACCCTTGGGACACATGTGCAGCAGTTCATGATGTGAGGCTTTCATTACTGCAGCGTTATTTTAAAGATAG TTCTTGCCTTTTGGCAGTATCAATTGGCTGGGAAGGAGGTGTTTATGTACAAACTTGTGGCCACACGTTG CACATAGATTGTCATAAATCCTATATGGAGTCGTTACGGAATGACCAGGTTCTTCAGGGCTTCTCAGTGG ACAAAGGAGAATTCACGTGTCCCCTCTGTAGACAGTTTGCCAACAGTGTTCTTCCATGTTATCCTGGAAG CAATGTGGAAAATAACCTTTGGCAACGCCCTTGTAACAAAAGCACGCAAGATCTCATAAAGGAAGTGGAA GAGCTGCAGGGACGGCCGGGAGCTTTCCCATCAGAAACCAACTTAAGTAAAGAAATGGAATCTGTAATGA AAGATATAAAAAATACCACTCAGAAGAAATACAGAGACTATAGCAAGACCCCAGGCTCACCAGACAACGA GTTTCTCTTTATGTACTCTGTTGCTAGAACCAATCTGGAGCTGGAACTGATTCATAGAGGAGGCAGTCTC TGCTCCGGTGGTCCAAGCACAGCGGGGAAAAGATCTTGTCTGAATCAGCTGTTCCATGTATTAGCCTTGC ACATGCGGCTTTATACCATTGATTCTGAATATAATCCATGGAAAAAACTCACCCAGTTAGTGGAAGATAT GAATTCACAGGTGGGGAATGAAGACCAGCAGCCAGAAGTTCCAATTCTTTATCATGATGTGACATCCCTT CTGCTCATCCAGATCTTAATGATGCCACAACCCTTACGTAAAGAGCACTTCACCTGCATTGTGAAAGTAC TCTTCACCCTGCTGTACACACAGGCCCTGGCGGCGCTCTCAGTTAAGGGCACTGAAGAGGACCGGTCAGC CTGGAAACACGCCGGGGCTCTTAGAAAGGATACATGTGATGCAGAAAAGTGTTACGAAGTGCTGTTGAGC TTTGTTATAAGTGAGCTGTCTAAAGGGAAGTTATATTATGAAGAAGGCGCTCAGGAGTGTGCGATGGTCA GTCCTATTGCTTGGTCTCCTGAGTCCATGGAAAGATACATACAGGATTTCTGCTTGCCTTTCCTCAGGGT CAGCAGCCTTCTTCAGCACCACCTCTTTGGGGAAGACTTACCCAGTTGCCAGGAAGAAGAAGAATTTTCA GTCCTTGCCAGCTGCCTGGGACTTCTGCCAACTTTTTACCAAACAGATCACCCGTTCATCAGTGCCTCGT GTCTGGACTGGCCAGTTGCAGCGTTTGACATTATAACTCAGTGGTGTTTTGAGATAACATCATTTACGGA GAGACACGCAGAGCAAGGAAAGGCCTTGCTTATCCAAGAATCAAGATGGAAGTTACCACACCTGTTACAG TTGCCTGAGAATTACAACACTATTTTTCAGTACTATCATAGAAAAACCTGCAGTGTCTGCACCAAGGTTC CTAAAGACCCAGCTGTTTGCCTCGTCTGCGGCACTTTCGTATGCCTGAAAGGCCTGTGCTGCAAGCAGCA AAGTTACTGTGAATGTGTGCTGCATTCTCAGAATTGTGGTGCAGGAACAGGAATTTTCCTTTTAATCAAC GCGTCAGTGATCATCATCATCCGAGGCCACCGCTTTTGCCTCTGGGGTTCCGTCTACTTGGATGCTCATG GAGAAGAGGACCGGGACCTCAGGCGAGGCAAGCCTCTCTACATCTGTGAGGAGCGGTACAGAGTTCTCGA GCAGCAGTGGGTTTCCCACACGTTTGACCACATCAATAAAAGATGGGGTCCGCACTACAATGGGCTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_177783
Insert Size	5670 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177783.6, NP_808451.2
RefSeq Size	8086 bp
RefSeq ORF	5670 bp
Locus ID	68795
UniProt ID	Q5U430
Gene Summary	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (PubMed:17462990). Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (PubMed:17462990). May play a role in Shh signaling by mediating the ubiquitination of Kif7 (PubMed:27195754). May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A (PubMed:27331610).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 3. This results in isoform (2) which is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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