Myo16 (NM_001081397) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BM140241; C230040D10Rik; Gm1105; mKIAA0865; NYAP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225001 representing NM_001081397 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATTGACCAGTGCTTGCTGGAGTCCCTCCCCCTGGGCCAGCGGCAGCGTCTGGTGAAGCGCATGC GCTGTGAGCAAATCAAAGCTTACTATGAGCGAGAGAAAGTGTTCCAGAAGCAGGAAGGGCCCCTGAAGAG AAGCAAGCCTGGGAAGAGGCAGAAGGTTCGCTTCGGCCTGGCTGACATGATTCAGGATGCTGTCATCCAC CATCATGACAAAGAGGTGCTTCAACTCTTGAAGGAGGGGGCAGATCCACACACTCTCGTGTCCTCAGGAG GGTCTTTGCTACACCTGTGTGCTAGATACGACAACGTCTTCATTGCAGAAGTCCTCATTGACAGGGGCGT CAATGTCAACCATCAGGATGAGGACTTTTGGACCCCAATGCACATTGCCTGTGCCTGTGACAACCCTGAC ATTGTCCTGTTGCTCATATTAGCTGGAGCCAACGTCTTTCTGCAAGATGTGAATGGAAACATCCCTTTAG ATTATGCCGTTGAGGGGACAGAATCTAGTGCTATCCTGCTGGCCTATCTGGATGAGAAAGGGGTGGACCT CAGCTCGCTGCGCCAGATAAAGCTGCAGCGACCCCTGAGTATGCTAACGGACGTCAGGCACTTCCTGTCT TCAGGCGGAGATGTCAACGAGAAAAATGATGATGGAGTGACCCTGCTGCACATGGCGTGTGCCAGTGGGT ATAAAGAGGTGGTATTGCTTCTCCTGGAACACGGTGGAGACCTCAATGGGACAGACGACAGGTACTGGAC ACCCCTGCACTTAGCAGCCAAGTATGGCCAGACAACCCTGGTGAAGCTCCTTCTGGCACATCAGGCAAAC CCCCACCTTGTGAACTGCAATGGGGAGAAACCATCAGACATCGCTGCCTCCGAGTCGATCGAGGAGATGT TGCTGAAAGCCGAAATCGCCTGGGAAGAGAAAATGAAAGAGTCTCCGTCAGCCCCCAGCTTAGCACAGGA AGAGCTGTACGAGATCCTTCATGACCTCCCAGATCTCTCCAGTAAGCTAAGTCCCCTGGTGCTACCAATC GCCAAGCAGGACAGCTTACTGGAAAAAGACATTATGTTCAAAGACACAACGAAAGGTCTGTGCAAGCAGG AGTCCCAGGATGGCCCTCCTGAGACCAGCATGACAAGCAACTGCGGCAAGCCAGAGCAGGTCCAGGTCAT GCCTCCAGCTCCCAGCGATGACCTAGCCACACTCAGTGAGCTTAATGACAGCAGCCTGCTGTATGAAATT CAGAAGCGCTTTGGAAATGACCAGATACACACCTTTATCGGAGACATCTTTTTGCTTGTTAACCCATTCA AAGAACTCCCAATTTACTCCACTATGGTGTCCCAGATGTATCTGAGCCCCACAGGCCAGCGCAGCCCCTC ACTGCCACCCCACCTCTTCTCCTGCGCAGAGAGAGCCTTCCACAGACTGTTCCAGGAGCGAAAGCCACAG AACATCATCCTCAGTGGAGAACGAGGGTCAGGGAAGACCCAGGCCAGCAAGCAGATCATGAAGTACCTGA CCAGCAGGGCCAGCTCCAGCTGTACCATGTTTGACTCCAGACTCAGGCATGCCATATACATCGTGGAGGC CTTCGGACATGCCAAGACCACACTCAACAATGTGTCCAGTTGCCTCATACAGTACTGGGAACTGCAGTGC TGTCAGAGGAGAAAGCACATAACTGGAGCCAGAATTTCCACTTACATGCTGGAGAAGTCCAGGGTTGTGG CTCAGCCTCCCGGCCAGGGCACCTTTCTGATTTTCTCCTGGTTGATGGACGGGTTATCTTCGGAAGAAAA ATGTGGACTTCACCTTAATAACTTCTGTGCACACCGGTATGTGAGCCAAGGCATGCGGGAAGATGTGTCT ACAGCAGAGCATTCTCTGAACAAGGAGAGACTTGCAGCTCTGAAACATGCTCTGAATGTAATTGGCTTCA GCACCTTGGAGGTGGAAAACCTATTCGTTATCCTATCGGCGATACTACACATCGGAGACATTCAGTTCAC TGCACTAACAGAAGCTGACTCGGCCTTTGTCTCTGACCTCCAGCTTCTGGAACAAGTGGCAGGTATGTTG CAAGTGTCGACCGACGAGCTGGCATCCGCCCTAACAACTGATATTCAGTATTTTAAAGGAGATGTGATAA TACGTCGACACACTATCCAGATGGCTGCGTTTTACCGCGACCTCCTGGCCAAGTCACTGTACAGTCGTTT GTTTGGCTTTCTGATAAACACTGTGAATTGCTGCCTCCAGAATCAAGATGAATATAAAAGCCTACAGACA TTGGATATTGGAATACTGGACATTTTTGGTTTTGAGGAATTTCAAAAGAATGAATTTGAACAACTCTGTG TCAACCTGACCAACGAAAAGATGCACCACTATATCCAAGAGGTGCTTTTCCTACAAGAGCAAACAGAGTG TGTACAAGAGGGAGTTGCCATGGAAACTGCCTGTTCTCCTGGTAACCAGGCTGGAGTTTTGGATTTCTTC TTCCAGAAACCATCAGGATTCTTCTCCTTACTGGATGAGGAGAGTCAAGTGATTTGGTCAGGGGAGCCAA ACCTCCCCAGAAAGCTGCAGGGTCTCCTGGAATCCTCAAACACAAATGCAGTCTACTCCCCTGTGAAGGA CGGCAATGGAAATGTTGCTTTCAAAGGTCAAGGTGCGGCCTTCACTGTCATGCACTACGCAGGAAGGGTC ATGTATGAAATGGGAGGAGCAGTTGAAAGAAACAAAGACTCTCTTTCACAGAACCTCTTATTTGTAATGA AAACAAGTGAAAATGTCGTGATCAGTCATTTGTTCCAGTCAAAGCTGTCACAAACAGGATCGCTCATATC TTCCTACCCATCCTTTAAATTTGGAGGACACAAATCCACCCTGCTTAGCAAGAGAACCGCCTCTTCCATG GTTGGAGTAAATAAGAATTACCTGGAACTTAGCAAGTTATTAAAAAAGAAGGGAACTTCCACATTCCTAC AAAGACTGGAACGAGGAGAGCCTGCCACCGCTGCATCACAACTCACGAAATCTCTGGCAGACATTACTGC GAAGCTGCAAAGGGGCAGCCCACACTTCATCCTTTGCATCAAGCCCAATACCTCGCAGCTGCCTGGTGTG TTTGATCACTTCTACGTGTCTGCCCAACTGCAGTACCTCGGTGTCCTGGGGCTGGTGAGGCTATTCCGCT CTGGGTATCCAGTGCGCCCTTCCTTCGAGGACTTCCTGTCCAGATACGAGCCACTGGCTTCTGTTCTTCT GGGCGAGACGAAGGGTCAGGCTGCTGAGGAGAGGTGTCGACTTGTTCTCCAGCGGTGTAAACTGCAAGGT TGGCAGATTGGAGTACACAAAGTGTTCCTGAAGTACTGGCACGTGGACCAGCTCAGCGACCTGTGGCTCC AGCTGCAAAGGAAGATTGTAACTTGCCAGAAAGTTATAAGAGGATTTCTGGCACGCCAGCACTTACTTCA GAGAATGAGCATCAAGCAACAAGAGGTTACATCCATCAAGAGCTTCTTACAGAGCACAGAGGACATGGCA CTGAAAACCTATGATGCCCTGGTCATTCAGAACGCTTCCGATATCGCCCGGGAACACGACAGGCTCCGGA AGGAGGTCCACACTGCCTACCACAGGAACAGACAAGAGGAAGGAACCAAGAGAGCTGAAGACCAAGGAGG ATGCAGGCACGTCCACTCCAACTCCGTGCCGGTGCCCATGGTAGTAGACAGCCTCGCCCAGGCTCTCACC GGACCATCTACCCGGCCCCCATCATTGCACTCTGTGTTCAGCATGGATGACAGCACTGGCCTCCCATCAC CACGGAAACAGCCTCCACCCAAGCCAAAGAGGGACCCCAACACCCGGCTGAGTGCCTCCTATGAGGCTGT GAGCGCCTGCCTTTCAGCTGCCAAGGATGCAGCCGGTGAAGCTCTGACACGGCCCAGACCCCACAGTGAT GACTATAGCACCATGAAGAAGATTCCCCCTCGGAAGCCAAAGAGAAGTCCCCACACGAAGCTCAGTGGCT CTTATGAGGAGATCTGGGGACCACCACGACCTTCTGGCACCATGGGCCAGGGGGGCAGGCATCAGGCCCC AGGGACACTGAGTGTGCAGTGGGCCAGGCCTGACTCAGTGCCGCAGTGCACACCACAGCTGCCTCTGCAC CTGCCATTGCCACAGGGCGACTATGACGATGATGCTGAGCCTGTGTACATCGAGATGGTGGGGAATGCAG CCAGGGCAGGTGGATCTGAGACCGACAGCCCTGACCAGGGCGAGTCTGTGTATGAGGAGATGAAGTACAT CTTGCCAGAGGAGGGCTGTGGCCTAGGGATGCTCACCTTCCTGCCTGCCTCACCTCCTTTGTTTTTAGAG ACTCGAAAAGCCATCATCTTGGAGGCTGCTGAGGGGAACTGCCAGCCCTCAAAGGATACATGTGACATCC CCCCACCCTTCCCCAATCTGCTTCCCCACAGGCCACCACTCCTAGTCTTTCCCCCCACCCCAGTGACCCG CTCCCCAGCTTCTGACGAGTCTCCACTGACACCCTTGGAGGTGAAGAAACTGCCAGTCTTAGAGACCAAC CTCAAGTACCCGGTGCAGTCTGAAGGCTCCAGTCCCTTGTCACCACAGTACTCAAAAGCACAGAAGGGAG ATAATGACCAGCTGGCATCCCCTGGCTTTCCTGTGTTTAATGGGCCCAGCCGAATCTCACCACCTGCCAC ACCACCGCCACCCCCAGGGCCACCTCCTGCACCCTGTGGGCCACCTCCTGCACCCTGTGGGCCACCCACC CACTTCGCCTTCCCACCAGAGTCTGTCCTGGTCACTGCAGCTAAAGCGCTGACTAACTCAGACCTGCCCA GAACACAGCCAAAGCCCAGTTCGGCCCCAGTACCGGGTCCCTGCAGCTCCTTTGTCAAGGCTCCATATTC ACCGGGAAAGACAGCCAGAGCAGACCTCAGGAAGACCTCATCCACCTTCTCTCCACCAAGCCCTTACAGC CCACCCAACAGCAGACCGCTGTCCAGTCCTCTGGACGAGCTGGCCAGCCTCTTCAACTCGGGAAGGAGTG TGCTACGCAGGTCTGCAGTGGGACGAAGGATCAGGGAGGCTGAAGGTTTTGAAACTAACATGAACCTGAG TAGCCGGGATGAACCCAGTTCATCAGAAATGGCTTCAGAGACTCAAGACAGAAACGCCAACAACCATGGG ACTCAACTCTCCAGTTCCCTGTCTAGCGATGTGACGGCTGAAAATGGAAATCCTGTCACCAATGGTTTAG CTGAGGACGATGGGTGCTCAAGGCTGTGTCTGAGTGGCATGGGGACTTCATCATTTCAGAGAAATCGGGA GAGCCACACCACCCAGGTTATCCATCAGCTGAGGCTCTCTGAGAATGAAAGCGTAGCTCTGCAGGAGCTG CTGGACTGGAGGAGGAAGCTCTGTGAGGCCAGAGAAGGCTGGCAGGAAGCCCTGCAGCACCCGGAGCCCC GTGCACCTCCGCCGCCACCCTGCAAGAAGCCGACACTCCTAAAGAAGCCTGAGGGGGGCTCCTGCACCAG GCTGCCTTCCCAACTCTGGGACAGCAGTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081397
Insert Size	5634 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081397.1, NP_001074866.1
RefSeq Size	6296 bp
RefSeq ORF	5634 bp
Locus ID	244281
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. May be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development (By similarity). Activates PI3K and concomitantly recruits the WAVE1 complex to the close vicinity of PI3K and regulates neuronal morphogenesis.[UniProtKB/Swiss-Prot Function]

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